脑红蛋白在内毒素脑损伤大鼠模型中的表达变化

Objective To investigate the expression of neuroglobin(Ngb) in frontal cortex, cere-brospinal fluid (CSF) and serum in rats with brain injury induced by LPS and to elucidate its significance. Methods Seventy adult Sprague-Dawley rats were randomly divided into LPS (n = 60, with injection of 0.1 mg/kg LPS into cistern) and control (C, n =10, with injection of equal volume of isotonic saline into cistern) groups, with 10 rats in each group. The plasma, CSF as well as frontal cortex from sacrificed rats were collected in LPS group at 3, 6, 12, 24, 48, 72 post injection hour (PIH), with 10 rats at each time point. The protein expression of Ngb was measured by enzyme-linked immunosorbent assay(ELISA) and Western blot. Expression of Ngb in frontal cortex was determined by SP. Water content of frontal cortex was assessed by drying method. The correlation among Ngb contents in serum, CSF, frontal cortex and water con-tent of frontal cortex was analyzed with multiple comparison. Results Ngb expression ( by ELISA) : Ngb expression of frontal cortex, CSF, and serum in LPS group was higher than that in C group at each time point, and it peaked at 48 PBH (35.4±3.9, 22.7±3.1, 14.4±2.8 ng/mL, respectively, P <0.01). Ngb ex-pression ( by Western blot) : Ngb protein with relative molecular mass of 17×103 was observed in each group. Ngb expression detected by Western blot was similar to that detected by ELISA. Brain water content was significantly greater in LPS group than that of C group at 6-72 PIH ( P<0.01) , and it peaked at 48 PBH ( 83.3±1.9) %. Ngb contents in serum, CSF, frontal cortex, and water content of frontal cortex were positively correlated with muhipie comparison (γ=0.631-0.719, P<0.01). Conclusions Up-regu-lation of Ngb expression in brain injury induced by LPS is correlated with duration after challenge of LPS.     

结合专业特色,提高护理解剖学教学效果

护理本科教育是培养护理人才的重要教育方式,护理解剖学教学是培养高级护理人才的重要课程之一,结合近年来护理解剖学教学过程中存在的问题,从师资、教材、课程建设、实验室建设和教学方法等方面,提出提高护理解剖学教学质量的几点初步设想。     

NGF及NGFR对雄性生殖系统的作用

１９５９年Ｌｅｖｉ -Ｍｏｎｔａｌｃｉｎｉ在蛇毒中首先发现神经生长因子 (ｎｅｒｖｅｇｒｏｗｔｈｆａｃｔｏｒ，ＮＧＦ) ,ＮＧＦ的发现被认为是神经科学发展史上的重要里程碑。通常认为 ,ＮＧＦ在特定阶段对特定的交感、感觉神经元和中枢神经系统某些胆碱能神经元的发育、分化、行使正常生理功能有重要的作用［１］。近年来发现ＮＧＦ不仅对神经系统有作用 ,而且对生殖系统、免疫系统、癌前病变及肿瘤组织等也具有十分重要的作用。雄性生殖器官中广泛存在ＮＧＦ及其受体 (ＮＧＦＲ) ,它们可能在雄性生殖系统的发育、形态、功能以及病理过…     

胰淀素在痴呆小鼠和正常小鼠脑内的分布和表达变化

目的 观察胰淀素在正常小鼠、阿尔茨海默病(Alzheimer's disease,AD)模型小鼠和肥胖模型小鼠脑内的分布和表达情况,分析其与小鼠脑内老年斑的关系,探讨胰淀素是否参与老年性痴呆的发病.方法 取5月龄雄性APP/PS1双转基因小鼠(AD模型小鼠)、ob/ob肥胖模型小鼠及野生型小鼠(正常小鼠),采用免疫荧光染色方法检测胰淀素和老年斑在各组小鼠脑内的分布和表达.结果 免疫荧光双标检测结果显示:胰淀素免疫阳性神经元广泛分布于APP/PS1双转基因小鼠脑内,包括皮质、海马、丘脑等区域,强阳性表达于岛叶和梨形皮质区域(P＜0.01),而老年斑则选择性表达于新皮质、海马等区域,胰淀素的分布范围较老年斑的分布广泛,在部分老年斑的周围可见胰淀素免疫阳性物质分布.5月龄AD模型小鼠、ob/ob肥胖模型小鼠新皮质内胰淀素阳性神经元数目明显高于正常小鼠(P＜0.05,P ＜0.01).结论 胰淀素在AD模型小鼠、ob/ob肥胖模型小鼠及正常小鼠脑内的分布和表达存在差异,这种差异有可能促进老年斑的形成以及参与AD疾病的发生.     

脑红蛋白在内毒素脑损伤大鼠模型中的表达变化

Objective To investigate the expression of neuroglobin(Ngb) in frontal cortex, cere-brospinal fluid (CSF) and serum in rats with brain injury induced by LPS and to elucidate its significance. Methods Seventy adult Sprague-Dawley rats were randomly divided into LPS (n = 60, with injection of 0.1 mg/kg LPS into cistern) and control (C, n =10, with injection of equal volume of isotonic saline into cistern) groups, with 10 rats in each group. The plasma, CSF as well as frontal cortex from sacrificed rats were collected in LPS group at 3, 6, 12, 24, 48, 72 post injection hour (PIH), with 10 rats at each time point. The protein expression of Ngb was measured by enzyme-linked immunosorbent assay(ELISA) and Western blot. Expression of Ngb in frontal cortex was determined by SP. Water content of frontal cortex was assessed by drying method. The correlation among Ngb contents in serum, CSF, frontal cortex and water con-tent of frontal cortex was analyzed with multiple comparison. Results Ngb expression ( by ELISA) : Ngb expression of frontal cortex, CSF, and serum in LPS group was higher than that in C group at each time point, and it peaked at 48 PBH (35.4±3.9, 22.7±3.1, 14.4±2.8 ng/mL, respectively, P <0.01). Ngb ex-pression ( by Western blot) : Ngb protein with relative molecular mass of 17×103 was observed in each group. Ngb expression detected by Western blot was similar to that detected by ELISA. Brain water content was significantly greater in LPS group than that of C group at 6-72 PIH ( P<0.01) , and it peaked at 48 PBH ( 83.3±1.9) %. Ngb contents in serum, CSF, frontal cortex, and water content of frontal cortex were positively correlated with muhipie comparison (γ=0.631-0.719, P<0.01). Conclusions Up-regu-lation of Ngb expression in brain injury induced by LPS is correlated with duration after challenge of LPS.     

局部解剖学实验教学改革初探

局部解剖学是培养医学生动手能力的一门重要课程。在面临扩招、尸源不足的情况下,局部解剖学课程究竟如何设置才能达到教与学的双赢,为配合局部解剖学精品课程建设工作的实施,对教学的各个环节进行认真审视,不断总结并改革局解实验教学方法和手段,充分利用各种教学资源,提高学生的综合素质,从而全面提升教学质量。     

脑红蛋白在脊髓缺血再灌注损伤中的表达及其意义

目的:观察大鼠脊髓缺血再灌注损伤(SCI)过程中脑红蛋白(Ngb)的表达变化及其意义。方法:健康成年Wistar大鼠随机分为脊髓压迫缺血再灌注组和假手术对照组。运用原位杂交组织化学和免疫印迹法,检测压迫段脊髓组织中Ngb的表达变化。结果:再灌注2h后,Ngb在压迫段脊髓开始表达上调,24h较为明显,48h达峰值,72h表达减少,与假手术组相比较,具有显著性差异。免疫印迹法的结果和上述结果一致。结论:在脊髓缺血再灌注损伤中Ngb的表达变化与再灌注时间相关,其表达上调是机体内源性神经保护之一。     

多媒体在电镜实习课教学改革中的应用

结合教学的实际情况,对本科生电镜课程实习教学方法进行了改革,除取材部分仍要求学生亲自动手外,主要采用图片阅读的多媒体实习方式。实践表明,这种实习教学方式有助于缓解实习课教学过程中的具体矛盾,同时也扩大了学生的知识面,取得了较好的教学效果,并对以后的教学方法改革提出了设想。     

红景天苷对大鼠缺血性脑水肿的影响

目的研究红景天苷(salidroside)对水通道蛋白4(aquaporin 4,AQP4)在大鼠缺血性脑组织中表达的影响。方法采用线拴法制作大鼠局灶性脑缺血模型,实验分为模型组、假手术组、红景天苷组。通过光镜技术观察各组别脑水肿区的病理变化,干湿质量法检测脑含水量,采用原位杂交、免疫组化方法观察AQP4及其mRNA在脑组织中的表达,硫代巴比妥酸法和定磷法测定缺血脑组织中丙二醛(MDA)、Na ,K -ATPase活性。结果缺血后模型组的细胞及血管周围间隙明显扩大,部分细胞坏死,神经胶质细胞增生;大鼠穹隆下器官、脉络丛、大脑皮质、视上核、海马等部位AQP4及其mRNA的表达上调,48～72 h达峰值;脑组织含水量亦在72 h达高峰。红景天苷组脑组织形态结构无明显改变,脑组织含水量、MDA水平、AQP4及其mRNA的表达明显低于模型组;而Na ,K -ATPase活力明显高于模型组。结论红景天苷可抑制缺血性脑水肿组织中AQP4及其mRNA的过表达,防止脑水肿的发生。