BMP-2对人退变椎间盘髓核细胞影响的实验研究

[目的]探讨BMP -2对人退变髓核细胞合成细胞外基质的影响.[方法]分离、培养人退变椎间盘髓核细胞,取第2代髓核细胞,随机将退变推间盘髓核细胞分为2组.A组:加入100 ng/ml BMP -2,B组:加入200 ng/mlBMP -2,C组:对照组,不加干扰因素.通过对试验组和对照组髓核细胞采用光镜、电镜等形态学方法进行大体形态和超微结构观察,细胞Ⅱ型胶原和糖胺多糖的mRNA表达.ELISA检测细胞培养上清中人Ⅱ型胶原含量,DMMB比色法检测细胞培养上清中糖胺多糖含量.[结果]髓核细胞中Ⅱ型胶原、糖胺多糖表达水平实验组均高于对照组.[结论] BMP-2蛋白可促进退变腰椎间盘细胞分泌蛋白多糖和Ⅱ型胶原,增加细胞活性,恢复椎间盘的功能和活性,因此运用BMP-2椎间盘内注射有望成为椎间盘退变疾病生物治疗的方法之一.     

伽马状交锁髓内钉治疗转子间粉碎性骨折

延伸至转子下的转子间粉碎性骨折是严重的转子骨折类型，约占转子骨折的１／３。如何处理好此型骨折的复位内固定，早期活动，仍是一个难题。作者自１９９３年６月～１９９６年６月应用伽马状交锁髓内钉治疗延伸至转子下的转子间粉碎性骨折５２例，获得较好的治疗效果，现...     

成人肱骨远瑞关节内骨折的手术治疗

成人脱骨远端关节内骨折的最佳治疗方法一直是众多学者讨论的焦点。作者通过对12例成人肱骨远端关节内骨折的治疗，体会到：早期手术、关节内解剖复位、坚强内固定及早期功能锻炼是获得好的治疗效果的关键。1临床资料自1996年5月～1998年10月对12例成人肱骨远瑞关节内骨折行手术治疗。其中男4例，女8例；年龄50～85岁，平均年龄6岁。按AO／ASIF分类法[1]分类；CI型3例，CZ型7例，C3型2例。合并肘关节脱位4例，开放性骨折3例。治疗方法：2例患者因同时合并高血压、糖尿病，须术前行内科治疗，遂于伤后3天手术。其余10例均于伤后队小时内…     

导航下与传统方法应用动力髋螺钉治疗老年股骨转子间骨折的疗效比较

Objective To compare the navigational and traditional dynamic hip screw (DHS) fix-ations in the treatment of intertrochanteric fractures in elderly patients. Methods A retrospective study was conducted to analyze the elderly patients with intertrochanterie fracture who had been treated in our de-partment from April 2005 to April 2007 and whose clinical data were complete. Enrolled in the present study were 25 cases that had received navigational DHS fixation and 31 cases who had received traditional DHS fixation. In navigational group, there were 10 males and 15 females, with an average age of 82. 1 years. They were 14 eases of Evans type Ⅰ and 11 Evans type Ⅱ. In traditional group, there were 12 males and 19 fe-males, with an average age of 82. 8 years. They were 16 cases of Evans type Ⅰ and 15 Evans type Ⅱ. The 2 groups were compared in incision length, operation time, bleeding volume, X-ray exposure time, bed-ridden time, medical and implant complications, and one-year functional recovery. Results In navigational group, 23 patients were followed for 11 to 20 (average, 14) months and 2 were lost. In traditional group, 30 patients were followed up for 14 to 23 (average, 18) months and 1 died from cardiovascular disease within 1 year. Compared with traditional group, navigational group had a smaller incision, less blood loss, shorter bed-ridden time and less X-ray exposure time, a higher rate of fracture healing at 3 months, with statistically significant differences (P <0.05). The Harris hip function scores at postoperative 1 year were of no significant difference between the 2 groups (P > 0.05) . In navigational group there were 3 medical and 1 implant complications. In traditional group there were 9 medical and 5 implant complications. There was no significant difference between the 2 groups (P > 0.05). Conclusion In the treatment of intertrochanteric fractures for the elderly patients, the navigational DHS fixation is superior to traditional DHS fixation due to its ad-vantages of smaller incision, less blood loss, shorter bed-ridden time, faster fracture healing and betterfunctional recovery.     

正常与退变椎间盘纤维环细胞相关基因的差异表达

目的: 研究人正常和退变椎间盘纤维环细胞椎间盘退变相关基因表达量的差异,探讨这些相关基因与椎间盘退变的关系,筛选明显差异基因作为退变纤维环细胞体外刺激因子,逆转椎间盘退变。方法: 实时荧光定量PCR(real-time qRT-PCR,SYBR Green)技术检测人正常和退变纤维环细胞TGF-β、IGF-1、bFGF、IL-1、TIMP-1、COL1A1、BMP-14、PDGF、aggrecan、COL2A1、SOX 9、BMP-2、iNOS、 EGF、 MMP3、BMP-4和BMP-7 mRNA表达水平。结果: 与正常纤维环细胞相比,退变纤维环细胞TGF-β、IL-1、PDGF和IGF-1 mRNA表达量降低(P<0.01,P<0.05);bFGF、TIMP-1、BMP-14和COL1A1 mRNA表达升量高(P<0.01);正常纤维环细胞aggrecan和BMP-2 mRNA高表达,退变纤维环细胞两者表达阴性;COL2A1、iNOS和MMP3在正常纤维环细胞mRNA有表达,在退变纤维环细胞表达阴性;EGF在正常纤维环细胞mRNA 表达阴性,而在退变纤维环细胞有表达。Sox-9、BMP-4和BMP-7在正常和退变纤维环细胞mRNA表达均为阴性。结论: 椎间盘纤维环退变与TGF-β、IL-1、PDGF、IGF-1、aggrecan和BMP-2 mRNA低表达,bFGF、TIMP-1、BMP-14和COL1A1 mRNA高表达相关;BMP-2、TGF-β、PDGF和IGF-1可作为退变纤维环细胞体外刺激因子,用于逆转椎间盘退变的研究;bFGF、TIMP-1和BMP-14 可能是椎间盘纤维环细胞退变的标志物。     

人端粒酶反转录酶重组绿色荧光表达载体转染椎间盘正常髓核细胞

背景：椎间盘髓核细胞分离培养困难,老化较快,迫切需要一种标准细胞株用于实验研究。目的：探讨人端粒酶反转录酶重组绿色荧光表达载体的构建及其转染正常髓核细胞构建永生化细胞的可行性研究。方法：通过目的基因克隆、真核表达质粒中目的基因序列测定、目的基因真核表达质粒的构建、转染人端粒酶反转录酶表达检测等步骤进行实验。结果与结论：构建出人端粒酶反转录酶重组绿色荧光表达载体,成功转染正常髓核细胞并在细胞中稳定表达。结果表明运用人端粒酶反转录酶转染椎间盘髓核细胞构建永生化细胞是一种可行的方法。     

不典型房室结折返性心动过速并左侧旁观者旁道一例

患者男性,48岁.因反复发作性心悸行电生理检查,心动过速发作时腔内A波激动顺序呈现由"偏心"转为"向心"的动态变化,变化过程中心动过速周长恒定,且希氏束电极上VA间期、HA间期及体表Q RS波形态均恒定不变,提示存在左侧旁道,且旁路传导与否并未影响折返环.诊断为不典型房室结折返性心动过速合并左侧游离壁旁观者旁道,实施旁...     

微创小切口治疗胫骨下段闭合性骨折

目的探讨微创小切口解剖型胫骨远端支持钢板治疗胫骨下段闭合性骨折手术治疗的应用。方法采用微创小切口解剖型支持钢板治疗43例胫骨下段闭合性骨折的病人,术后早期功能锻炼。结果全部病例获得随诊,平均愈合时间16周。术后X线检查显示复位和内固定位置满意,骨折全部愈合。按Klemm功能恢复分级标准,优23例,良15例,可5例。结论应用微创外科技术治疗闭合性胫骨粉碎骨折,可以达到医源性创伤小,骨折固定牢靠和早期功能锻炼的目的,具有一定的临床应用价值。     

关节腔冲洗和点穴舒筋法治疗膝骨性关节病102例

膝关节骨性关节病是中老年人的一种常见病、多发病,以膝关节疼痛、活动不利及膝关节畸形为主要临床表现.临床对该病的治疗,以理疗、按摩、中草药外洗及局部封闭为主.笔者自1993年3月～1996年应用关节腔冲洗与点穴舒筋法结合治疗本病,取得了较好的疗效,总结报告如下.     

闭合复位髓内钉治疗股骨转子间骨折的临床分析

目的：探讨髓内钉治疗转子间骨折的疗效及并发症的分析。方法：回顾性分析我院2006年5月～2010年3月使用股骨近端髓内钉治疗转子间骨折163例。股骨颈基底骨折14例,转子下骨折10例,转子间骨折139例;按E-van＇s分型,Ⅰ～Ⅳ型分别为15、54、63、31例。其中应用Gamma钉37例、股骨近端重建钉74例、股骨近端钉52例。结果：术后随访1～4年,根据Harris髋关节功能判定标准,优良率为81.4%。结论：股骨近端髓内钉在治疗股骨转子间骨折效果满意,具有固定牢固、创伤小的特点。