心肌梗死后室间隔穿孔的手术时机探讨

目的:分析心肌梗死后室间隔穿孔手术围术期死亡的危险因素,寻找室间隔穿孔手术的最佳手术时机.方法:回顾性分析中国医学科学院阜外医院2006年1月至2020年12月间连续收治的112例行手术治疗的室间隔穿孔患者的临床及随访资料.根据术后30 d死亡情况及心肌梗死位置进行分组,分别分析生存组与死亡组、前壁心肌梗死组与下壁或后...     

窄带成像技术下NICE分型对直乙结肠微小息肉的诊断效果分析

目的:分析窄带成像技术(NBI)下NICE分型对直乙结肠微小息肉的诊断效果.方法:选取2019年1月—2020年4月本院接收的83例疑似直乙结肠微小息肉患者,先给予83例患者窄带成像技术检查,再行手术病理检查,对窄带成像技术下NICE分型诊断效果进行分析.结果:经窄带成像技术检查,发现83例患者存在168处直乙结肠息肉...     

蛋白激酶C在调节血管内皮细胞通透性中的作用

蛋白激酶C(protein kinase C,PKC)是一种钙或/和磷脂依赖性蛋白磷酸化酶,广泛存在于人体的各种组织细胞中.它能被细胞外生物活性因素(生长因素、神经递质、细胞因子等)激活,完成靶细胞蛋白的磷酸化,通过蛋白磷酸化后生物活性的改变而完成细胞外源性信号的应答,构成细胞内重要的信号转导系统.许多实验证明蛋白激酶C的激活在血管内皮细胞屏障功能和血管通透性的变化调节中起着重要的作用.本文将就PKC在血管通透性变化中的效应、激活方式、信号的通路以及作用底物等方面做一综述.     

Effect of structural domain Ⅱ of HCV 5' noncoding region on its translation initiation activity

Objective To examine the role of domain Ⅱ of hepatitis C virus(HCV) 5'noncoding region (5'NCR) in its translation initiation activity. Methods The fragment of HCV 5' NCR with deletions of 5'-proximal 118 nucleotides were amplified with PCR, then was used to substitute for the full length HCV 5'NCR of plasmid pCMVNCRIuc, a firefly luciferase(Flue) eukaryotic expression plasmid regulated by HCV 5' NCR, to generate recombinant plasmid pCN1-d3, pCMVNCRIuc, pCN1-d2 (modified pCMVNCRluc with deletions of HCV 5' NCR nt1-43) and pCN1-d3 were transfected into HepG2 cells with liposome transfection protocol, respectively, and the relative luciferase activity was measured to analyze the regulatory effect of the truncated 5'NCR on Fluc gene expression. Meanwhile the Fluc mRNA levels were detected by RT-PCR. Results The recombinant plasmid was successfully constructed. The Fluc mRNA levels of the 3 plasmids were not significantly different(P 0.05), and there were also no significant difference between the relative luciferase activity of plasmids pCN1-d2 and pCMVNCRluc(P 0.05). However, that of pCN1-d3 was decreased significantly(P < 0.01, compared with pCN1-d2 or pCMVNCRluc). Conclusion Structural domain Ⅱ of HCV 5' NCR plays an important role in itstranslation initiation activity.     

Effect of structural domain Ⅱ of HCV 5' noncoding region on its translation initiation activity

Objective To examine the role of domain Ⅱ of hepatitis C virus(HCV) 5'noncoding region (5'NCR) in its translation initiation activity. Methods The fragment of HCV 5' NCR with deletions of 5'-proximal 118 nucleotides were amplified with PCR, then was used to substitute for the full length HCV 5'NCR of plasmid pCMVNCRIuc, a firefly luciferase(Flue) eukaryotic expression plasmid regulated by HCV 5' NCR, to generate recombinant plasmid pCN1-d3, pCMVNCRIuc, pCN1-d2 (modified pCMVNCRluc with deletions of HCV 5' NCR nt1-43) and pCN1-d3 were transfected into HepG2 cells with liposome transfection protocol, respectively, and the relative luciferase activity was measured to analyze the regulatory effect of the truncated 5'NCR on Fluc gene expression. Meanwhile the Fluc mRNA levels were detected by RT-PCR. Results The recombinant plasmid was successfully constructed. The Fluc mRNA levels of the 3 plasmids were not significantly different(P 0.05), and there were also no significant difference between the relative luciferase activity of plasmids pCN1-d2 and pCMVNCRluc(P 0.05). However, that of pCN1-d3 was decreased significantly(P < 0.01, compared with pCN1-d2 or pCMVNCRluc). Conclusion Structural domain Ⅱ of HCV 5' NCR plays an important role in itstranslation initiation activity.     

蛋白激酶C在调节血管内皮细胞通透性中的作用

蛋白激酶Ｃ(ｐｒｏｔｅｉｎｋｉｎａｓｅＣ ,ＰＫＣ)是一种钙或 /通过激活ＰＫＣ来增加内皮细胞的通透性 ,同时伴有细胞间连接缝隙增宽[2 ] 。而ＰＫＣ活性的抑制剂如隙形成 ,导致通透性的升高[9] 。2　参与调节内皮细胞通透功能的ＰＫＣ亚型和激活通路 ,引起蛋白质如细胞骨架蛋白的磷酸化 ,最终导ＤＡＧ再分别激活以Ｃａ2 +和ＰＫＣ为代表的两条信号ＰＫＣ活化和Ｃａ2 +升高 ;再由Ｃａ2 +与钙调蛋白结合形成Ｃａ2 +-ＣａＭ ,激活ＭＬＣＫ ;最后由ＭＬＣＫ介导ＭＬＣ蛋白激酶C在调节血管内皮细胞通透性中的作用@杨滔$第一军医大学病理生理…     

Effect of structural domain Ⅱ of HCV 5' noncoding region on its translation initiation activity

Objective To examine the role of domain Ⅱ of hepatitis C virus(HCV) 5'noncoding region (5'NCR) in its translation initiation activity. Methods The fragment of HCV 5' NCR with deletions of 5'-proximal 118 nucleotides were amplified with PCR, then was used to substitute for the full length HCV 5'NCR of plasmid pCMVNCRIuc, a firefly luciferase(Flue) eukaryotic expression plasmid regulated by HCV 5' NCR, to generate recombinant plasmid pCN1-d3, pCMVNCRIuc, pCN1-d2 (modified pCMVNCRluc with deletions of HCV 5' NCR nt1-43) and pCN1-d3 were transfected into HepG2 cells with liposome transfection protocol, respectively, and the relative luciferase activity was measured to analyze the regulatory effect of the truncated 5'NCR on Fluc gene expression. Meanwhile the Fluc mRNA levels were detected by RT-PCR. Results The recombinant plasmid was successfully constructed. The Fluc mRNA levels of the 3 plasmids were not significantly different(P 0.05), and there were also no significant difference between the relative luciferase activity of plasmids pCN1-d2 and pCMVNCRluc(P 0.05). However, that of pCN1-d3 was decreased significantly(P < 0.01, compared with pCN1-d2 or pCMVNCRluc). Conclusion Structural domain Ⅱ of HCV 5' NCR plays an important role in itstranslation initiation activity.     

蛋白激酶C在中毒性休克中调节内皮细胞通透性的机制研究

目的：蛋白激酶Ｃ（ＰＫＣ）通道是一条重要的细胞内信号传导通道，许多实验证明蛋白激酶Ｃ的激活在调节内皮屏幕功能中起者重要的作用。静息情况下，ＰＫＣ绝大多数位于细胞的胞浆部分，胞膜部分含量很少，当细胞受到某些刺激（如抗原或某些炎性介质）之后，胞膜后的磷脂崩解产生二酰基甘油（ＤＡＧ），ＤＡＧ的增加可激活胞浆中的ＰＫＣ，使其从胞浆转位至胞膜，这种转位是ＰＫＣ活化的重要标志。有研究表明ＰＫＣ只有在活化状态才     

老年病人髋关节置换术后的并发症及预防

目的:探讨老年病人髋关节置换术后常见并发症及其预防方法.方法:回顾性分析老年全髋关节置换术患者71例的临床资料,总结其术后常见并发症及其预防方法.结果:71例均顺利完成手术,无死亡病例,术后无患者发生感染、关节脱位等并发症,切口甲级愈合.结论:老年病人髋关节置换术后常见并发症,包括假体脱位、感染、下肢深静脉血栓形成、神经血管损伤、压疮等,是可以通过合理的方法预防的.