全文获取类型
收费全文 | 76篇 |
免费 | 5篇 |
国内免费 | 2篇 |
专业分类
基础医学 | 16篇 |
临床医学 | 5篇 |
内科学 | 4篇 |
特种医学 | 10篇 |
外科学 | 18篇 |
综合类 | 13篇 |
预防医学 | 1篇 |
药学 | 10篇 |
中国医学 | 6篇 |
出版年
2023年 | 1篇 |
2022年 | 2篇 |
2021年 | 3篇 |
2020年 | 1篇 |
2018年 | 1篇 |
2017年 | 1篇 |
2016年 | 2篇 |
2015年 | 4篇 |
2014年 | 4篇 |
2013年 | 7篇 |
2012年 | 4篇 |
2011年 | 5篇 |
2010年 | 2篇 |
2009年 | 9篇 |
2008年 | 3篇 |
2007年 | 9篇 |
2006年 | 7篇 |
2005年 | 4篇 |
2004年 | 1篇 |
2003年 | 1篇 |
2002年 | 4篇 |
2001年 | 4篇 |
2000年 | 4篇 |
排序方式: 共有83条查询结果,搜索用时 0 毫秒
21.
Objective To examine the role of domain Ⅱ of hepatitis C virus(HCV) 5'noncoding region (5'NCR) in its translation initiation activity. Methods The fragment of HCV 5' NCR with deletions of 5'-proximal 118 nucleotides were amplified with PCR, then was used to substitute for the full length HCV 5'NCR of plasmid pCMVNCRIuc, a firefly luciferase(Flue) eukaryotic expression plasmid regulated by HCV 5' NCR, to generate recombinant plasmid pCN1-d3, pCMVNCRIuc, pCN1-d2 (modified pCMVNCRluc with deletions of HCV 5' NCR nt1-43) and pCN1-d3 were transfected into HepG2 cells with liposome transfection protocol, respectively, and the relative luciferase activity was measured to analyze the regulatory effect of the truncated 5'NCR on Fluc gene expression. Meanwhile the Fluc mRNA levels were detected by RT-PCR. Results The recombinant plasmid was successfully constructed. The Fluc mRNA levels of the 3 plasmids were not significantly different(P 0.05), and there were also no significant difference between the relative luciferase activity of plasmids pCN1-d2 and pCMVNCRluc(P 0.05). However, that of pCN1-d3 was decreased significantly(P < 0.01, compared with pCN1-d2 or pCMVNCRluc). Conclusion Structural domain Ⅱ of HCV 5' NCR plays an important role in itstranslation initiation activity. 相似文献
22.
23.
目的 对中国重庆青少年特发性脊柱侧凸(AIS)进行遗传流行病学调查,以确定遗传因素在发病中的作用及其遗传模式.方法 采用传统的家系研究方案设计遗传流行病学调查表,调查73例先证者家系,分析年龄发病率、家族聚集性和遗传度,确定遗传因素在发病中的作用,依据各种遗传病的特点分析其遗传模式.结果 73例先证者中男25例,女48例,共有家系成员1 550人,其中76人发病.年龄发病率分析显示75%的患者在11~14岁发病,平均发病年龄10.8岁;调查家系总发病率为4.90%,而参照群体平均发病率为2.25%(P<0.01),提示本病有家族聚集性;先证者一级亲属遗传度84%,二级亲属遗传度88%,三级亲属遗传度85%.综合三项结果提示本病为一种遗传性疾病.对有家族史的家系进行分析显示,本病不具有各种单基因遗传病的特点;先证者一级亲属发病率16.93%,二级亲属发病率7.30%,三级亲属发病率4.36%,均高于参照人群发病率(2.25%);同胞发病率0.0%,远比1/2或1低;与先证者亲缘关系越近,发病率越高;有家族性聚集倾向;家族史发生率4.11%.结论 AIS是一种多基因遗传病. 相似文献
24.
A new, convenient, and rapid method for kinetic measurement of human fibrinolysis was established. The alteration of absorbance (A) in the process of blood coagulation and lyses was auto-matically scanned and recorded using a UV2000 spectrophotometer connected to a computer. The parameters of human fibrinolysis kinetics were established. Urokinase at 20 U/mL was the optimal concentration used. There was significant difference in fibrinolysis kinetics and plasma plasminogen concentration between 22 normal subjects and 27 patients with acute myeloblastic leukemia (P<0.05 and <0.01 respectively). The coefficience of variation was (5.24±1.51)%. This method could also be used to measure the plasma fibrinogen concentration at the same time. It was concluded that this method was stable and was capable of providing dynamic, direct experimental data and multipareme-ters for clinicians. It was also valuable in evaluating the anti- and pro-fibrinolytic capcity of patients' plasmas, allowing for monitoring of therapy, choice of drugs and adjustment of drug concentrations. 相似文献
25.
Objective To examine the role of domain Ⅱ of hepatitis C virus(HCV) 5'noncoding region (5'NCR) in its translation initiation activity. Methods The fragment of HCV 5' NCR with deletions of 5'-proximal 118 nucleotides were amplified with PCR, then was used to substitute for the full length HCV 5'NCR of plasmid pCMVNCRIuc, a firefly luciferase(Flue) eukaryotic expression plasmid regulated by HCV 5' NCR, to generate recombinant plasmid pCN1-d3, pCMVNCRIuc, pCN1-d2 (modified pCMVNCRluc with deletions of HCV 5' NCR nt1-43) and pCN1-d3 were transfected into HepG2 cells with liposome transfection protocol, respectively, and the relative luciferase activity was measured to analyze the regulatory effect of the truncated 5'NCR on Fluc gene expression. Meanwhile the Fluc mRNA levels were detected by RT-PCR. Results The recombinant plasmid was successfully constructed. The Fluc mRNA levels of the 3 plasmids were not significantly different(P 0.05), and there were also no significant difference between the relative luciferase activity of plasmids pCN1-d2 and pCMVNCRluc(P 0.05). However, that of pCN1-d3 was decreased significantly(P < 0.01, compared with pCN1-d2 or pCMVNCRluc). Conclusion Structural domain Ⅱ of HCV 5' NCR plays an important role in itstranslation initiation activity. 相似文献
26.
27.
内毒素(LPS)主要激活PMNs和内皮细胞,通过复杂的信号转导通路,引起微血管内皮细胞屏障功能和形态的损伤,使血管通透性增加,血容量下降,是导致内毒素性休克和多器官功能损害的重要原因. 相似文献
28.
Objective To examine the role of domain Ⅱ of hepatitis C virus(HCV) 5'noncoding region (5'NCR) in its translation initiation activity. Methods The fragment of HCV 5' NCR with deletions of 5'-proximal 118 nucleotides were amplified with PCR, then was used to substitute for the full length HCV 5'NCR of plasmid pCMVNCRIuc, a firefly luciferase(Flue) eukaryotic expression plasmid regulated by HCV 5' NCR, to generate recombinant plasmid pCN1-d3, pCMVNCRIuc, pCN1-d2 (modified pCMVNCRluc with deletions of HCV 5' NCR nt1-43) and pCN1-d3 were transfected into HepG2 cells with liposome transfection protocol, respectively, and the relative luciferase activity was measured to analyze the regulatory effect of the truncated 5'NCR on Fluc gene expression. Meanwhile the Fluc mRNA levels were detected by RT-PCR. Results The recombinant plasmid was successfully constructed. The Fluc mRNA levels of the 3 plasmids were not significantly different(P 0.05), and there were also no significant difference between the relative luciferase activity of plasmids pCN1-d2 and pCMVNCRluc(P 0.05). However, that of pCN1-d3 was decreased significantly(P < 0.01, compared with pCN1-d2 or pCMVNCRluc). Conclusion Structural domain Ⅱ of HCV 5' NCR plays an important role in itstranslation initiation activity. 相似文献
29.