缺血性脑卒中患者凝血因子Ⅰ浓度与血小板平均体积的动态变化

目的 探讨缺血性脑卒中凝血因子Ⅰ（Fg）的浓度与血小板平均体积（MPV）的动态变化规律，以及在早期预防和临床治疗中的理论根据。方法 回顾性对照分析45例缺血性脑卒中患者不同时期凝血因子Ⅰ（Fg）浓度及MPV指标变化及与临床进展的相关性。结果 缺血性脑卒中患者不同时期MPV与Fg水平随着病情恢复，由急性期的高水平逐渐减低，且显著高于对照组，呈明显正相关。结论 缺血性脑卒中时MPV升高与体内纤溶活性增强密切相关，也反映缺血性脑卒中危险性的大小，因此临床应对上述指标较高患者加强监测及临床干预治疗，以防止缺血性脑卒中的发生及复发。     

二维功率正态变换模型在血压日节律研究中的统计学方法:在中医处方临床第一阶段研究中的应用

在临床降压药物的研究中,生物日节律变化经常是作为一种评价药物效果的指标,24小时的血压测量比零散的血压测量更能体现患者的真实情况。对于测定生理节律的数值模型,有基于观察噪声为正态分布的周期回归模型和基于功率正态变换的Box-Cox变换。此次提出了一种二维功率正态变换模型以测定血压的日节律,其克服了以上模型     

胸水中多胺的高效液相色谱测定

本文采用丹酰氯试剂作为柱前衍生剂，用反相高效液相色谱法检测胸水中多胺的浓度。实验条件：荧光激发波长３４０ｎｍ，发射波长５１５ｎｍ。液动相为甲醇－水，本法测得多胺的回收率为：腐胺９３％￣１０７％，精脒８９％￣１０３％，精胺９８％￣１０５％。变异系数分别为：腐胺７．４％￣９．５％，精脒４．３％￣６．１％，精胺３．３％￣４．２％，方法稳定，可靠。     

烧伤后大鼠肌组织唾液酸含量的变化

利用大鼠３０％Ⅲ度烧伤模型，探讨了烧伤大鼠心肌组织唾液酸含量的变化。同时还观察了心肌组织三磷酸腺苷酶活性和Ｃａ＾２＋含量改变。结果表明：烧伤后大鼠心肌组织唾液酸显著降低，伤后３小时仅为对照组的５８．８％，同时心肌组织Ｎａ＾＋，Ｋ＾＋－ＡＴＰａｓｅ，Ｍｇ＾２＋－ＡＴＰａｓｅ和Ｃａ＾２＋－ＡＴＰａｓｅ活性降低，而Ｃａ＾２＋含量明显升高。     

经颅磁刺激联合度洛西汀对脊髓损伤后神经病理性疼痛及情绪障碍的影响

目的：探讨重复经颅磁刺激(rTMS)联合度洛西汀对脊髓损伤后神经病理性疼痛及情绪障碍的影响。方法：选取在我院住院的脊髓损伤后神经病理性疼痛患者40例,随机分成刺激组和对照组,每组20例。两组患者均给予常规康复治疗药物治疗,对照组选用度洛西汀治疗配合伪rTMS治疗,刺激组在给予度洛西汀同时配合rTMS治疗。两组患者均于治疗前及治疗4周后采用视觉模拟量表(VAS)、汉密尔顿焦虑/抑郁量表(HAMA/HAMD)进行评估,并对比两组不良反应发生情况。结果：治疗前两组患者的VAS评分、HAMA评分、HAMD评分无统计学差异(P>0.05);治疗4周后,两组VAS评分、HAMA评分、HAMD评分均较治疗前改善,且刺激组优于对照组(P<0.05)。两组不良反应发生情况比较差异无统计学意义(P>0.05)。结论：rTMS联合度洛西汀治疗可进一步改善脊髓损伤患者神经病理性疼痛及焦虑抑郁情绪。     

Ionotropic glutamate receptor subunit distribution on hypoglossal motoneuronal pools in the rat

The expression of ionotropic glutamate receptor subunits in the motoneuronal pools of the hypoglossal nucleus was studied using specific antibodies against subunits of the -amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA), kainate and N-methyl-D-aspartate (NMDA) subtypes. The highest numbers of intensely immunolabelled motoneurons were found in the dorsal tier and caudoventromedial part of the hypoglossal nucleus with all antibodies except that against the GluR1 AMPA subunit. Labelling for the GluR1 subunit was weak except for caudally located groups of motoneurons which innervate tongue muscles related to respiratory activity. By contrast, most motoneurons were intensely immunostained with antibodies against GluR2/3 and GluR4 subunits of the AMPA subtype. The low staining observed using an antibody specific for the GluR2 subunit (which prevents Ca²⁺-entry through AMPA channels) strongly suggests that AMPA receptors in hypoglossal motoneurons are Ca²⁺-permeable. Immunolabelling for the GluR5/6/7 kainate receptor subunits was found in many motoneuronal somata as well as in thin axon-like profiles and puncta that resembled synaptic boutons. Most motoneurons were intensely immunostained for the NMDA receptor subunit NR1. These results show that the hypoglossal nucleus contains five heterogeneous pools of motoneurons which innervate functionally defined groups of tongue muscles. The uneven expression of the different receptor subunits analysed here could reflect diverse phenotypic properties of hypoglossal motoneurons which might be expected to generate different patterns of motor responses under different physiological or pathological conditions.     

Lymphoepithelioma-like carcinoma of the bladder: three cases with clinicopathological and p53 protein expression study

Lymphoepithelioma-like carcinoma of the bladder is an uncommon neoplasm, of which 49 cases have been described in the English literature, none of which has been studied for p53 protein expression. We studied three muscle-infiltrating cases of this tumor using immunohistochemical, in situ hybridization and polymerase chain reaction (PCR) methods. The three cases were positive for epithelial markers and negative for lymphoid antigens in the tumoral syncytial areas. The intensive infiltrate of small cells was negative for epithelial and positive for lymphoid markers. This population was mainly made up of cytotoxic T-lymphocytes, positive for TIA-1. p53 protein was intensely positive in more than 90% of the epithelial component nuclei, being negative in the lymphoid cells. PCR study did not show mutations on p53. Both lymphocytes and epithelium were negative for Epstein–Barr virus markers, such as the latent membrane protein and EBER (Epstein–Barr-encoded RNA). The prognosis was very good after radiotherapy and chemotherapy treatment, preserving the bladder despite the muscle infiltration. The presence of an intense cytotoxic T-lymphocyte population may be related to this good prognosis. Both aspects, p53 protein status and T-lymphoid population, had never been studied before in bladder lymphoepithelioma-like carcinoma.     

器官移植供体器官摘取术中肾及相关结构损伤防护问题

目的:探讨有关供体多器官摘取过程中肾及相关结构的损伤防护问题。方法:回顾性分析2003年11月~2004年12月间进行的30例供体器官摘取过程,分析发生肾及相关组织损伤的原因。结果:其中单侧取肾法4例,肝肾联合摘取法26例。发生双侧肾包膜(纤维膜)撕裂2例,肾动、静脉横断(左侧)1例,肠秽污染1例。双肾完整切取26例。单侧取肾法热缺血时间(8.2±3.0)min,肝肾联合摘取方法双肾热缺血时间(1.8±0.9)min。结论:通过操作方法及设备的改进,供体多器官摘取过程中的意外肾损伤是可以避免的。     

Prosthetic mitral valve endocarditis due to Ochrobactrum anthropi: case report

We describe a case of infective endocarditis in a prosthetic mitral valve due to Ochrobactrum anthropi. Although O. anthropi is an emerging pathogen in immunocompromised patients, infections with the bacterium have very rarely been documented in healthy hosts, and endocarditis is rare. To our knowledge, only two cases of O. anthropi endocarditis have been reported in the medical literature.