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精液液化异常与精浆脂蛋白a的检测和分析 总被引:1,自引:0,他引:1
目的:通过对男性不育症患者精液液化异常的精浆进行脂蛋白a检测和分析,探讨精浆脂蛋白a对精液液化异常的影响及临床意义。方法:按照WHO《人类精液检查与处理实验室手册》第5版标准,对101例精液液化异常者、26例精液液化正常对照者的精液进行常规分析,再加糜蛋白酶于精液中置于37℃孵育30 min,待挑起完全无丝状物后离心取上层精浆检测脂蛋白a。结果:在101例精液液化异常者中精浆脂蛋白a水平[(526.2±243.5)mg/L]明显高于26例精液液化正常对照组精浆脂蛋白a水平[(296.9±105.2)mg/L](P<0.01)。结论:机体脂蛋白a具有抑制纤维蛋白溶解功能,精液液化异常中纤维蛋白的溶解延迟可能与精浆脂蛋白a水平增高有一定相关性。临床诊断精液液化异常引起的男性不育症时,应考虑精浆脂蛋白a水平的影响。 相似文献
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目的 观察常见风湿性疾病血液中多项炎症指标,分析比较其在疾病过程中的临床意义及相关性,探讨选择敏感简便快捷的检测指标作为临床对病程的监测和疗效的观察.方法 检测132例常见风湿性疾病活动期的免疫球蛋白IgG、IgA、IgM,补体C3、C4,C-反应蛋白、红细胞沉降率、白细胞、血小板参数,并与经治疗后的风湿性疾病稳定期和健康体检者进行比较和统计学处理分析.结果 风湿性疾病活动期C-反应蛋白、血小板分布宽度、大血小板比率明显高于稳定期和恢复期(P<0.01).结论 血小板分布宽度、大血小板比率与C-反应蛋白和风湿性疾病活动期有显著相关性,可作为风湿性疾病活动期的疗效观察、病程监测的一个敏感检验指标. 相似文献
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目的 探讨应激系统对缺血预适应中心肌细胞凋亡的影响.方法 以家兔在体心经典缺血预适应(IPC)实验为基础,增设结扎肾上腺组(Lig组),米非司酮组(皮质醇受体水平阻滞剂)(MF组),甲基强的松龙组(MP组),以TUNEL法,DNA ladder及流式细胞仪(FASCan)检测凋亡的存在及凋亡率,同时检测血清皮质醇动态变化,考察应激启动的皮质醇分泌对缺血预适应心肌细胞凋亡的影响.结果 TUNEl染色显示MP组和IPC组比较凋亡率差异没有显著性意义(5.05±2.25 % vs 3.46±0.82 %,P>0.05),MF组和Lig组与IPC组比较差异有显著性意义(7.73±3.08 %,16.62±12.35 % vs 3.46±0.82 %,P<0.05);DNA ladder半定量显示MP组和IPC组电泳条带明显减少;流式细胞仪检测显示Lig组,MF组心肌细胞凋亡较IPC组差异有显著性意义(3.82±1.38 %,3.22±0.67 % vs 1.72±0.2 9%,P<0.05),MP组与IPC组比较差异没有显著性意义(2.04±0.74 % vs 1.72±0.29 %).结论 从细胞凋亡角度观察IPC的心肌保护作用,结扎肾上腺,受体水平阻滞皮质醇作用可明显削弱其保护作用,MP可一定程度模拟其保护作用,说明应激系统的部分组分通过影响IPC的心肌细胞凋亡起到其保护作用. 相似文献
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Objective To investigate the antibacterial activity of antibacterial substance in Lucilia seri-cata induced by pricking the skin of maggots(fly s larvae)at different pH conditions. Methods The hemo-lymph from the third instar maggots was collected 48 h after pricking,and the antibacterial effect was tested a-gainst Micrococcus lysodeikticus and Staphylococcus aureus on plates at different pH conditions.The antibacterial effect against M.1ysodeikticus was also detected after the hemolymph、was diluted by different pH buffer solu-tions.The bacteriostatic effects were observed after 24 h and the diameters of clear inhibitory ring were recorded to express antibacterial activity. Results The antibacterial activity of the maggot hemolymph against M.lyso-deiktics and S.aureus was stronger on the plates at pH 6.0 and pH 6.5 than that on the other plates.In the group diluted by pH 6.0 buffer solutions.the antibacterial ring of the maggot hemolymph against M lysodeiktic-us was larger than that in others. Conclusion The results implied that the induced antibacterial substance in Lucilia sericata exhibited stronger antibacterial activity under acidic condition. 相似文献
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Objective To investigate the antibacterial activity of antibacterial substance in Lucilia seri-cata induced by pricking the skin of maggots(fly s larvae)at different pH conditions. Methods The hemo-lymph from the third instar maggots was collected 48 h after pricking,and the antibacterial effect was tested a-gainst Micrococcus lysodeikticus and Staphylococcus aureus on plates at different pH conditions.The antibacterial effect against M.1ysodeikticus was also detected after the hemolymph、was diluted by different pH buffer solu-tions.The bacteriostatic effects were observed after 24 h and the diameters of clear inhibitory ring were recorded to express antibacterial activity. Results The antibacterial activity of the maggot hemolymph against M.lyso-deiktics and S.aureus was stronger on the plates at pH 6.0 and pH 6.5 than that on the other plates.In the group diluted by pH 6.0 buffer solutions.the antibacterial ring of the maggot hemolymph against M lysodeiktic-us was larger than that in others. Conclusion The results implied that the induced antibacterial substance in Lucilia sericata exhibited stronger antibacterial activity under acidic condition. 相似文献
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Objective To investigate the antibacterial activity of antibacterial substance in Lucilia seri-cata induced by pricking the skin of maggots(fly s larvae)at different pH conditions. Methods The hemo-lymph from the third instar maggots was collected 48 h after pricking,and the antibacterial effect was tested a-gainst Micrococcus lysodeikticus and Staphylococcus aureus on plates at different pH conditions.The antibacterial effect against M.1ysodeikticus was also detected after the hemolymph、was diluted by different pH buffer solu-tions.The bacteriostatic effects were observed after 24 h and the diameters of clear inhibitory ring were recorded to express antibacterial activity. Results The antibacterial activity of the maggot hemolymph against M.lyso-deiktics and S.aureus was stronger on the plates at pH 6.0 and pH 6.5 than that on the other plates.In the group diluted by pH 6.0 buffer solutions.the antibacterial ring of the maggot hemolymph against M lysodeiktic-us was larger than that in others. Conclusion The results implied that the induced antibacterial substance in Lucilia sericata exhibited stronger antibacterial activity under acidic condition. 相似文献
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目的了解乙型肝炎(简称乙肝)肝癌患者的流行病学特征并观察其血清学转换和乙型肝炎病毒DNA(HBV-DNA)变化趋势,探索乙肝病毒血清学转换与肝癌预后的关系。方法收集襄阳地区两家三甲医院乙肝肝癌患者病历,统计其吸烟史、饮酒史、家族史、感染乙肝时间,HBV-DNA定量检测结果和HBV血清学标志物检测结果,对仍在院的乙肝肝癌患者,用荧光定量PCR检测HBV-DNA水平和酶联免疫吸附测定法检测HBV血清学标志物。结果1475例乙肝肝癌患者中男性明显多于女性,51~60岁的人数最多;乙肝肝癌患者感染乙肝时间大多分布于10~<20年;HBV-DNA阳性率为61.47%,且HBV-DNA定量检测结果多位于103~106 copies/mL;最常见的乙肝“两对半”模式是“小三阳”;抗-HBs阳性的患者也不少,占12.17%;2013-2016年,HBV-DNA定量检测结果不同区间变化趋势不同。结论性别、年龄、家族史、感染时间是乙肝肝癌的高危因素。肝癌患者HBV-DNA检测结果多为阳性,需定期监测HBV-DNA载量并综合乙肝“两对半”及肝功能结果来指导抗病毒治疗。 相似文献
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