颅脑疾病患者血清及脑脊液中心肌酶谱检测的意义

目的观察各种心肌酶在颅脑疾病患者血清及脑脊液中的变化,为临床颅脑疾病的鉴别诊断提供依据。方法采集结核性脑膜炎、病毒性脑膜炎、脑血管疾病、急性颅脑损伤和脑肿瘤患者的血清及脑脊液,检测其乳酸脱氢酶(LDH)、谷丙转氨酶(ALT)、谷草转氨酶(AST)、羟丁酸脱氢酶(HBDH)、肌酸激酶(CK)和谷氨酰转移酶(GGT)值。结果 1)与健康对照组比较,各组脑脊液及血清中的心肌酶差异有统计学意义,均有不同程度升高(P0.05),其中以急性颅脑损伤组最高;2)结核性脑膜炎患者与病毒性脑膜炎患者比较,脑脊液中的GGT、LDH、HBDH、CK差异有统计学意义(P0.05)。结论同时检测脑脊液及血清中的心肌酶可用于鉴别诊断病毒性脑膜炎和结核性脑膜炎;脑脊液中心肌酶的测定可作为急性颅脑损伤的性质及预后的辅助诊断指标。     

颅脑疾病患者血清及脑脊液中心肌酶谱检测的意义

Objective To investigate the Clinical Significance of myocardium enzymatic activity levels in serum and Cerebrospinal Fluid(CSF)from patients with brain disease.Methods Serum and CSF were collected from patients with Tuberculous Men-ingitis(TM)、 virus meningitis( VM)、Cerebrovascular Disease(CD)、 Acute Brain's Hurt ( ABH)and cerebroma(CER),then myocardium enzymatic were measured by chemistry colorimetry.Results 1) Serum and CSF of enzymatic activity were higher in every group patients with brain diseases comparing with normal group,significantly higher in ABH group;2)GGT、LDH、HBDH、CK of CSF in TM group was significantly higher than that of VM group.Conclusion Both myocardium enzymatic activity levels of serum and CSF can be used to distinguish TM and VM;myocardium enzymatic activity level in CSF are helpful to the prognostic diagnosis of ABH.     

荧光定量PCR检测乙型肝炎患者血清中HBV DNA的临床意义

目的检测乙型肝炎患者血清中乙型肝炎病毒(HBV)DNA的含量,了解其与免疫学指标的关系。方法采用荧光定量PCR技术和ELISA法,分别检测287例乙型肝炎患者血清中HBVDNA含量和免疫学指标。结果287例乙型肝炎患者血清中检出HBVDNA199例,阳性率为69.34%(199/287);144例HBsAg、HBeAg、抗HBc三项阳性的血清,其血清中HBVDNA也全部阳性;而HBsAg、抗HBe、抗HBc三项阳性和HBsAg、抗HBc二项阳性的标本,HBVDNA的检出率分别38.9%(42/108)和37.1%(13/35)。结论乙型肝炎患者血清中HBVDNA的拷贝数与HBeAg密切相关,但系统转换不能说明HBV停止复制,而只是复制水平的降低而已。     

RA患者抗天然Ⅱ型胶原抗体与ESR、CRP、AAG之间的关系

为探讨抗天然Ⅱ型胶原抗体(抗Nat Ⅱ)在类风湿性关节炎(RA)疾病活动中的意义.采用ELISA检测抗Nat Ⅱ IgG,用BN prospec特定蛋白分析仪检测C-反应蛋白(CRP)和α1-酸性糖蛋白(AAG),Electa血沉仪测定红细胞沉降率(ESR).结果60例RA患者血清抗Nat Ⅱ IgG水平与ESR和血清CRP、AAG含量呈正相关(相关系数r分别为0.402、0.268和0.382),其中23例抗NatⅡIgG阳性患者的ESR、CRP、AAG均显著高于抗Nat Ⅱ IgG阴性者(P＜0.01).抗Nat Ⅱ IgG可作为RA疾病活动性指标之一.