Over-expression of VEGF in marrow stromal cells promotes angiogenesis in rats with cerebral infarction via the synergistic effects of VEGF and Ang-2

This study explored whether the transplantation of modified marrow stromal cells (MSCs) has angiogenic effects in a left middle cerebral artery occlusion infarction/reperfusion (MCAO I/R) rat model and preliminarily examined the mechanism of angiogenesis following cerebral infarction.MSCs were isolated by using a direct adherent method and cultured.Vascular endothelial growth factor (VEGF) was transfected into MSCs by employing the liposome transfection.The transfection efficiency was measured by the optical density method.The protein expression of VEGF gene before and after transfection was measured by Western blotting.SD rat model of transient occlusion of the left middle cerebral artery was established by using an approach of intra-luminal occlusion.Tetrazolium (TTC) and HE staining were performed to observe the cerebral infarction.ELISAs were used to measure the levels of VEGF in the rat cerebral tissues.The expression patterns of angiopoietin-2 (Ang-2) and CD34 in cells surrounding the area of infarction were immunohistochemistrically oserved.Ang-2 protein expression in the tissue surrounding the area of infarction was measured by Western blotting.VEGF expression in the MSCs increased after transfection at a rate of approximately 28%±3.4%.ELISA showed that the expression of VEGF in the cerebral tissue was significantly increased after induction of infarction,peaking on the 4th day and decreasing to the levels of the sham surgery group (normal) within 7 to 10 days.The VEGF level was significantly higher at each time point in the VEGF-MSC and MSC groups compared to the model group.Moreover,the VEGF level was higher in the VEGF-MSC group than in the MSC group and stayed relatively high until the 10th day.The immunohistochemical results showed that 10 days after the infarction,the number of Ang-2 and CD34-expressing cells in the area surrounding the infarction was significantly higher in the VEGF-MSC group and the MSC group compared to the model group.Moreover,the VEGF level was higher in the VEGF-MSC group th     

舒适护理在剖宫产手术室护理中的应用分析

目的 分析在剖宫产手术室护理中采取舒适护理的临床护理效果和价值。方法 选取 2020 年 8 月至 2021 年 6 月我院收治 的 100 例剖宫产分娩的产妇作为研究对象。随机将产妇分为及对照组和实验组，每组各 50 例。对照组实施常规手术室护理， 实验组实施舒适护理。分析对比两组的护理满意度、舒适程度、心理状态及疼痛情况。结果 实验组的护理满意度显著高于对 照组（P<0.05）。实验组的舒适程度显著高于对照组（P<0.05）。观察组的疼痛程度显著低于对照组（P<0.05）。实验组心理状态 显著优于对照组（P<0.05）。结论 在剖宫产手术室护理中采取舒适护理，能提高护理满意度和舒适度，改善疼痛程度和心理状 态。