某工厂105名炊事员乙型肝炎三个抗原抗体系统的调查

乙型肝炎三个抗原抗体在不同人群中的阳性率国内外均有报告。但至今国内在同一健康人群中同时检测乙型肝炎表面抗原抗体(HBsAg、抗-HBs)、e抗原(HBeAg)、e抗体(抗-HBe)和核心抗体(抗-HBc)的报告尚较少见。为探讨乙型肝炎三个抗原抗体在同一人群中的阳性率及其相互关系,并了解炊事员中乙型肝炎病毒的携带和感染情况,我们于1981年7～8月进行了此项调查,现报告如下。     

荧光定量PCR法检测呼吸道感染患儿支原体、衣原体感染

目的:了解呼吸道感染患儿肺炎支原体、解脲脲原体、沙眼衣原体的感染情况。方法:应用荧光定量聚合链式反应法对571例呼吸道感染患儿同时进行肺炎支原体、解脲脲原体、沙眼衣原体检测。结果:肺炎支原体、解脲脲原体、沙眼衣原体总检出率为56.0％,其中肺炎支原体为29.1％,解脲脲原体为23.3％,沙眼衣原体为8.2％,肺炎支原体和解脲脲原体为3.2％,解脲脲原体和沙眼衣原体为1.4％。在年龄分布上,<28 d组患儿解脲脲原体感染率高达30.3％,且与其他组的差异有显著性,1个月-1岁组、>1-3岁组和>3岁组的患儿肺炎支原体感染率明显高于<28 d组,差异有显著性。肺炎支原体感染率四季差异无显著性,解脲脲原体冬春感染率高,沙眼衣原体春季感染率低。血和痰两种类型标本,肺炎支原体与解脲脲原体阳性率之间差异有显著性,与沙眼衣原体阳性率之间差异无显著性。结论:新生儿较其他年龄段患儿易感解脲脲原体、沙眼衣原体;肺炎支原体随着年龄的增长感染率逐渐增高。痰标本的阳性率明显高于血液。荧光定量聚合链式反应法诊断支原体、衣原体感染快速、敏感,特异性高。     

危重症新生儿胃黏膜及血液相关指标检测的临床意义探讨

2003年1月至2004年7月,我们对27例危重症新生儿的胃黏膜及血液相关指标进行了分析,旨在了解各参数在新生儿危重症疾病中的变化规律,探讨各指标评价病情危重程度及预测预后的特异性、敏感性,并提出导致疾病恶化的预警范围。     

南京地区急性呼吸道感染患儿人博卡病毒的检出与分析

目的 了解南京地区急性呼吸道感染患儿HBoV的检出情况,并探讨HBoV与临床特征的关系.方法 收集2009年7月至2010年6月南京医科大学附属南京儿童医院呼吸科收治的397例急性呼吸道感染住院患儿为病例组,对照组为50例无呼吸道感染症状的儿童.应用实时荧光定量PCR法检测鼻咽分泌物标本HBoV.对HBoV阳性标本,应用实时荧光定量PCR法检测MP和CT,直接免疫荧光法检测RSV、ADV、IVA、IVB、PIV-1、PIV-2、PIV-3和hMPV.随机选取5份HBoV阳性标本扩增HBoV NP-1片段后进行核苷酸序列测定,结果与GenBank中已知序列进行比对,绘制系统进化树.结合HBoV阳性患儿临床资料,分析HBoV的流行病学特点、临床表现和最终临床诊断.结果 实时荧光定量PCR法检出HBoV DNA阳性率为8.3%(33/397),其中有57.6%(19/33)混合其他病原体感染.与HBoV混合感染的前3位病原体依次为MP(27.3%,9/33)、RSV(24.2%,8/33)和PIV.3(12.1%,4/33).7～36个月龄感染HBoV患儿有25例,占HBoV DNA阳性患儿的75.8%(25/33).5份标本HBoV NP-1基因序列均一致,与st1、st2和WHL-1等序列同源性为99%～100%.结论 HBoV是南京地区急性呼吸道感染患儿的病原之一.HBoV NP-1基因高度保守,在不同地区和不同时期的流行株间变异较小,可作为实时荧光定量PCR等方法的检测标记.

Abstract：

Objective To investigate the possible existence of HBoV in children with acute respiratory infections in Nanjing area and explore its relationship with clinical characteristics.Methods A total of 397 nasopharyngeal secretion samples were collected from children with acute respiratory infection,admitted from July 2009 to June 2010 in Nanjing Children'S Hospital affiliated to Nanjing Medical University,and 50 cases of children without symptoms of respiratory infection were recruited as control group,whose nasopharyngeal secretion samples were also collected.HBoV was determined by real-time fluorescence quantitative PCR.MP and CT were detected by real-time fluorescence quantitative PCR in those HBoV-positive samples.RSV,ADV,IVA,IVB,PIV-1,PIV-2,PIV-3 and hMPV were detected by direct antigen-specific immunofluorescence assays.HBoV NP-1 fragments were amplified and sequenced in 5 HBoV positive samples randomly selected.The results were compared with the known GenBank sequence,and thereby the phylogenetic tree was established.The epidemiological characteristics,clinical presentation and the final clinical diagnosis of HBoV were analyzed according to the clinical data of the HBoV-positive patients.Results Thirty-three HBoV-positive cases were detected by real-time fluorescence quantitative PCR method with a positivity rate of 8. 3% ( 33/397 ). Among the 33 HBoV-positive cases, 19 cases (57.6%) were multiple infections with HBoV and other pathogens, the top three of which were MP (27.3% ,9/33 ),RSV (24.2% , 8/33 ) and PIV-3 ( 12. 1% ,4/33 ). Affected children aged from 7 to 36 months old accounted for 75.8% of the total ( 25/33 ). The measured HBoV NP-1 gene sequences of 5 specimens were consistent,indicating a high homology (99% to 100% ) with the stl, st2 and WHL-1. Conclusions HBoV is one of the pathogens of children's acute respiratory infections in Nanjing. HBoV NP-1 gene is highly conserved,with little variation in different seasons and in different regions and therefore can be used as a marker for real-time fluorescence quantitative PCR and other methods.