GX－Ⅱ型多功能电离子手术治疗机治疗32例化脓性肉芽肿临床疗效观察

ＧＸ－Ⅱ型多功能电离子手术治疗机治疗３２例化脓性肉芽肿临床疗效观察山东平度市皮肤病防治站张亚利，郭洪春，刘瑞英治疗方法：采用ＧＸ－Ⅱ型多功能电离子手术治疗机，首先将预先选好的针头套入针杆内，将开关拨向短头档，调节旋纽，使输出指示在１２～１３Ｖ之间。皮...     

大学新生的自杀意念与抑郁情绪、希望特质

目的:探讨大学新生自杀意念的检出率,以及与抑郁情绪、希望特质之间的关系。方法:选取某大学一年级全部新生4372名,采用症状自评量表(SCL-90)第15项评估自杀意念,采用抑郁自评量表(SDS)、希望量表(DHS)评估抑郁症状和希望特质。收回有效问卷4211份。结果:大学新生自杀意念发生率为4.5%(189/4211),女生自杀意念率高于男生(6.7%vs.3.7%,P0.05)。有自杀意念学生的SDS得分高于无自杀意念者[(42.0±7.3)vs.(34.2±6.3),P0.05],DHS得分低于无自杀意念者[(43.6±8.8)vs.(49.8±8.1),P0.05]。SDS得分越高,越容易产生自杀意念(OR=1.15,P0.01);而DHS得分越高,自杀意念产生的可能性越小(OR=0.95,P0.05)。结论:本研究发现提示大学新生自杀意念的产生可能与抑郁情绪相关,而希望特质可能是保护因素,对有自杀意念的大学生应重视抑郁情绪的评估。     

延庆 联合公安、城管取缔一家非法收售药品黑窝点

本刊讯 7月28日,北京市药监局延庆分局与公安、城管部门联合执法,共同查获一起非法收售药品的黑窝点。现场查封非法收购的药品25种,共计48盒,案值金额184元。     

Clinical Presentation and Treatment Strategies for Ulcerative Colitis: A Retrospective Study of 247 Inpatients

Objective

Complementary and alternative medicine, particularly herbal therapy, is widely used by patients with ulcerative colitis (UC), but controlled data are limited. To describe the clinical presentation and treatment strategies for UC in inpatients from Shanghai, China and to improve the therapeutic outcomes for patients with UC.

Methods

Medical records from 247 patients with UC who were admitted to Longhua Hospital Affifiliated to Shanghai University of Traditional Chinese Medicine between January 2008 and June 2013 were analyzed for gender, age, course of the disease, clinical type, extent and severity of the disease, treatment strategies, and therapeutic outcomes.

Results

Gender ratios and disease onset of inpatients with UC in the Shanghai area were consistent with other reports in the literature. In contrast to previous studies, most patients exhibited disease of the left colon, over half of the patients had problems of the rectum or sigmoid colon, and most patients had either mild or moderate UC. Comparison of Sutherland Disease Actirity Index scores for patients treated with Chinese medicine (CM) and those treated with integrated CM and Western medicine revealed signifificant reductions in scores for both groups after treatment (P<0.01), with no signifificant difference in therapeutic effects between groups (P=0.938).

Conclusions

Herbal medicine has been widely used in patients with mild to moderate disease and as adjunct therapy in patients with moderate to severe disease. Therefore, the strategy was proposed for the treatment of UC with CM therapy based on 2 steps according to the stage of the disease, even in the clinical setting.

     

miR-21靶向Atg5对非小细胞肺癌A549细胞自噬调控促进细胞增殖、迁移和侵袭的实验研究

目的  探讨miR-21通过靶向作用自噬相关靶基因5（Atg5）调控非小细胞肺癌（NSCLC）自噬的作用机制及其在A549细胞增殖、迁移及侵袭中的作用。方法  无义核酸序列NC（NC组）、miR-21 模拟物（miR-21 mimics组）、miR-21抑制物（miR-21 抑制组）分别转染A549细胞, CCK-8检测细胞增殖情况;划痕实验检测细胞迁移能力; Transwell侵袭实验检测细胞侵袭能力。双荧光素酶报告实验验证miR-21和Atg5之间的靶向关系。Western blotting检测LC3B-II、p62和Atg5蛋白的表达。结果  与NC组比较,miR-21 mimics组细胞增殖、迁移、侵袭能力均上调,miR-21 抑制组细胞增殖、迁移、侵袭能力均下调（P＜0.05）。双荧光素酶报告实验结果显示,miR-21显著抑制野生型Atg5 3’-UTR质粒转染细胞的荧光素酶活性（P＜0.05）,但对突变型Atg5 3’-UTR的基因报告质粒与miR-21 mimics共转染之后,并未对荧光素酶活性产生影响。NC组LC3B-II蛋白表达量为1.24±0.059,低于miR-21 mimics组的1.98±0.077,高于miR-21抑制组的0.52±0.021（P＜0.05）;NC组p62蛋白表达量为0.62±0.021,高于miR-21 mimics组的0.45±0.020,低于miR-21抑制组的0.79±0.031（P＜0.05）;NC组Atg5蛋白表达量为1.17±0.025,高于miR-21 mimics组的0.38±0.014,低于miR-21抑制组的1.40±0.039（P＜0.05）。与NC组比较, 3-MA处理降低miR-21 mimics转染诱导的A549细胞增殖能力（P＜0.05）;划痕实验和Transwell实验表明,3-MA处理抑制了miR-21mimics转染诱导的A549细胞的迁移和侵袭,差异有统计学意义（P＜0.05）。结论  miR-21靶向Atg5调控NSCLC自噬促进细胞增殖、迁移和侵袭。     

公众对自杀的态度量表的编制及在社区和大学学生中的应用

目的:编制公众对自杀的态度量表(the Scale of Public Attitudes about Suicide,SPAS),并在社区和大学学生中检验其信效度。方法:采用多阶段分层随机取样,抽取山东农村居民604例、天津城市居民548例及重庆的大学学生626例,用SPAS进行调查。首次调查后5～8天,顺序选择1063例被试进行重测。将受教育程度≥9年(n=580)的社区居民随机分为自评(n=243)和由调查员提问并填写问卷(n=337),将自评的大学学生随机分为署名(n=321)和匿名(n=305)。结果:(1)SPAS包含47个条目,分为7个分量表。(2)自杀问题的社会重要性分量表的内部一致性和重测一致性偏低(Cronbachα=0.48,ICC=0.59);其他6个分量表的内部一致性α在0.62～0.87之间,重测一致性ICC在0.62～0.82之间。(3)农村居民、城市居民和学生之间,除对自杀的正性态度分量表得分外,其余6个分量表得分的差异均有统计学意义(均P<0.05),农村组得分最高而学生组得分最低。自评组和由调查员提问并填写组相比,除自杀行为的自我不可控制性和对自杀的正性态度2个分量表外,其余5个分量表得分均是后者高于前者(均P<0.05)。署名和匿名组各分量表得分差异均无统计学意义(均P>0.05)。结论:公众对自杀的态度量表是一个可靠的评估国内社会对自杀的态度的工具。     

溃疡性结肠炎的中医药治疗

中医认为,溃疡性结肠炎的发生与六淫邪气、饮食不节、情志内伤及先天禀赋不足有关.中医药治疗常用的给药途径是中药口服、中药保留灌肠、中药栓剂及各种方法的联合应用,治疗上具有多途径、多方法、多靶点等特点和毒副作用小,费用低等优势.