Hyperoxia-induced apoptosis of AEC Ⅱ in premature rats by inhibiting expression of focal adhesion kinase

Objective To explore the role of focal adhesion kinase (FAK) in hyperoxia-apopto- sis of type Ⅱ alveolar epithelial cells (AEC Ⅱ s) of premature rats. Methods AEC Ⅱ from prema- ture rat lungs were cultured and randomly assigned to air group and hyperoxia group. After exposed to hyperoxia for 6, 12, 24 and 48 h, apoptosis rate of AEC Ⅱ were analyzed by flow cytometry with an- nexin-Ⅴ/propidium iodine (PI) double staining. FAK mRNA and FAK and fAK-Tyr397 peptide were detected by RT-PCR and Western blot, respectively. Results Positive cells of Annexin-Ⅴ+ / PI- in AEC Ⅱ after 6,12,24 and 48 h of hyperoxia exposure were significantly decreased, and the maximal apoptosis rate of AEC Ⅱ (stained of Annexin-Ⅴ+ / PI- ) was found in the hyperoxia group at 12 h (23.83%±4.43%). Compared to the air group, the expression of FAK mRNA and of FAK de- creased markedly and progressively in hyperoxia groups at 12, 24 and 48 h(P<0. 05). Conclusions Decreased expression of FAK induced by hyperoxia is likely to contribute to the apoptosis and neco-z sis of AEC Ⅱ.     

条件致病菌在骨伤科感染中的作用--附2947份细菌培养及药敏分析

为临床了解条件致病菌在骨伤科感染的作用及耐药情况。对住院治疗的 416 9例骨伤科患者的分泌物、脓液、抽出液进行了细菌培养、分离鉴定 ,并将得到的条件致病菌对常用抗生素的耐药情况进行统计总结。结果 2 947份有细菌生长 ,分离出细菌30 6 0株 ,其中条件致病菌 2 137株 ,占 6 9.84%,明显高于化脓性球菌和其他细菌的感染。在 2 137株条件致病菌中 ,以铜绿假单胞菌、变形杆菌、克雷伯氏菌属、肠杆菌属几种最为常见 ,通过对其药敏试验情况看 ,条件致病耐药性较强 ,以铜绿假单胞菌耐药性最强 ,在对常用 16种抗生素试验中耐药率 5 0 %以上有 11种 ,耐药率 90 %以上有 4种。认为条件致病菌在骨伤科感染中不但感染率高 ,且耐药性强 ,应引起临床的足够重视。     

先天性心脏阻滞与结缔组织病

先天性心脏阻滞不常见,约为活产新生儿的1/20,000。其中30％合并心血管畸形,70％为单一病变。本病患儿的母亲常有结缔组织病,系统性红斑狼疮占1/3。1954年Mecuistin首次报道系统性红斑狼疮患者分娩的新生儿有红斑狼疮症状,此后有不少类似报道。Hardy等,总结新生儿红斑狼疮54例,其中31例有心脏阻滞。新生儿红斑狼疮的临床表现常于生后2～3月消退,而心脏阻滞则多为永久性损伤。结缔组织的患者的后代易患先天性心脏阻滞的机理尚不明确。一股认为与母亲的自身抗体通过胎盘引起胎儿心脏传导系统的免     

川崎病的冠状动脉损伤

川崎病（ＫＤ）由日本川崎富作医师１９６１年首次发现,并于１９６７年发表５０例报告。起初认为本病是一种婴幼儿热性病,仅少数死亡病例经尸检证实有冠状动脉瘤（ＣＡＡ）。随着临床研究的深入,发现不少无症状的康复患儿冠状动脉造影有动脉瘤,这种致命性的心血管并发...     

侧脑室体外引流加脑脊液置换治疗脑室铸型出血16例报告

我院自 1992年 ,应用锥颅双侧脑室体外引流配合腰椎穿刺及脑脊液置换术治疗 16例全脑室铸型出血患者 ,取得满意疗效。1　资料和方法1 1　资料　 16例患者 ,男 11例 ,女 5例。年龄 45～ 75岁。平均63岁。既往有高血压病史者 9例。按 1995年全国脑血管疾病会议修订标准 ,所有患者神经功能缺损积分为 2 9～ 40。均经颅脑ＣＴ证实为全脑室出血形成铸型或全脑室并蛛网膜下腔出血。1 2　方法　所有患者入院后除采用脱水、利尿、控制血压等常规治疗外 ,均行锥颅穿刺侧脑室外引流术及腰穿脑脊液置换。具体操作方法 :常规备皮、消毒、铺无菌洞巾。选…     

原发性甲状腺功能减退症15例误诊分析

原发性甲状腺功能减退症（甲减）的误诊多见报道,多由于临床症状的非特异性及临床医生缺乏经验而误诊。为再次提高对本病的认识,进一步探讨本病的发病情况及临床特点,现将我院自１９９２年４月～１９９８年１０月收治的甲减２８例,其中误诊１５例的情况分析如下。１临床资料１．１一般情况：共１５例中,男３例,女１２例,年龄１８～６２岁,平均 ３８．４岁,病程半年至８年,其中半年～２年４例,～４年８例,～８年３例,平均５．６年。１．２临床表现：具典型粘液性水肿面容５例,凹陷性水肿１２例,重度贫血４例,轻中度贫血１１…     

书讯

据美国《针剌法》杂志报道,澳大利亚医学针灸及资源协会将于一九八三年十一月二十五日至二十九日在新加坡召开"第二届澳大利亚国际现代医学针灸专题讨论会".该专题讨论会将对古老的传统针刺法进行审查并将其与电生理学、神经生理学及生物化学方面的当代科学概念联系起来.     

肺透明膜病早产儿尸检肺组织肺表面活性蛋白-C及Ki67的表达

Objective To determine the expression of surfactant protein-c (SP-C) and Ki67 in autopsy lung tissues of premature infants died of hyaline membrane disease (HMD) who were exposed to mechanical ventilation and high oxygen concentrations/pulmonary surfactant (PS). The possible influence of surfactant on the expression of SP-C and Ki67 was also investigated. Methods Thirty preterm infants diagnosed as HMD by clinical data and pathology were selected. Mechanical ventilation and supplemental oxygen (FiO2 0.6-1.0) were given to these infants within 6 hours after birth. According to the duration of ventilation at high oxygen concentrations, all subjects were diveded into four groups: ventilation for 1-3 days, 4-8 days, 9-16 days and >16 days. Five premature infants died within 1 day after delivery for none pulmonary reasons served as controls. The expression of SP-C and Ki67 in lungs were detected by immunobistochemistry. Results The pulmonary pathology in these HMD infants at different phases was consisitent with the main features of different stages during the progress from HMD towards bronchopulmonary dysplasia. The positive staining of SP-C was restricted to type Ⅱ alveolar epithelial cells, and Ki67 positive were preferentially localized in nuclei of alveolar and bronchiolar epithelial cells and fihroblasts. Compared with the control group, the expression of SP-C and Ki67 in HMD infants decreased significantly after 1-3 days of ventialation, but increased after 4 days and reached the peak value after 9-16 days. The expression of SP-C and Ki67 in control group, 1-3 days and 9-16 days of ventialation were 0. 1891±0. 0253, 0. 1576±0. 0327 and 0. 2271±0. 0238 for SP-C and 0. 2297±0. 0380, 0.1929±0. 0403 and 0. 2849±0. 0368 for Ki67, respectively. No significant difference in the expression of SP-C and Ki67 was found between infants treated with PS and those without (P>0.05). Conclusions SP-C and Ki67 may have participated in the pulmonary pathological process in ventilated/oxygen treated preterm infants with HMD, and exogenous surfactant had no effect on the expression of SP-C and Ki67.