丹参和β—七叶皂苷钠对烧伤后外周血白细胞粘附聚集的调节

目的：探讨丹参和β-七叶皂苷钠对烧伤后外周血白细胞粘附聚集（LAA）的影响及LAA与肺组织损伤的关系。方法：采用大鼠30%TBSAⅢ度烫伤模型。45只大鼠随机分为假烫组，盐水组、丹参组、β七叶皂苷钠组及联合用药组，烫伤后24h取静脉血检测外周血白细胞粘附聚集（LAA）。取肺组织检测髓过氧化物酶（MPO）含量。结果：盐水组，丹参组、β-七叶皂苷钠组及联合用药组外周血LAA、肺组织MPO均升高，盐水组升高最明显，联合用药组最低，外周血LAA与肺组织MPO含量呈正相关。结论：LAA可反映中性粒细胞（PMN）在肺内的聚集程度，丹参及β-七叶皂苷钠均可降低外周血LAA的粘附，聚集，减轻肺损伤，联合用药效果更佳。     

肝复康对PDGF介导的肝星状细胞信号转导的分子机制

目的：通过观察中药肝复康含药血清对肝星状细胞（HSC-T6）细胞株Ⅰ、Ⅲ型胶原、α-SMA基因表达的影响。探讨其抗纤维化的作用机制。方法: 常规方法制备肝复康含药血清，并用不同浓度肝复康含药血清干预HSC-T6细胞株，以逆转录聚合酶链反应（RT-PCR）观察分析Ⅰ、Ⅲ型胶原、α-SMA mRNA表达的变化。以Western blotting检测ERK蛋白在不同干预情况下的表达。结果: 经肝复康含药血清处理，可下调HSC-T6细胞株Ⅰ、Ⅲ型胶原、α-SMA mRNA表达以及ERK蛋白表达水平，不同浓度含药血清作用强度也有所不同。结论: 中药肝复康对肝纤维化具有明显的抑制作用，其作用机制可能是通过非特异性干扰PDGF功能，抑制Ⅰ、Ⅲ型胶原、α-SMA mRNA的转录，以达到抗纤维化的作用。     

RNA干扰对小鼠腹腔活化巨噬细胞分泌TNF-α的影响

[目的]探讨RNA干扰对小鼠腹腔活化巨噬细胞表达TNF-α的抑制作用。[方法]原代培养小鼠腹腔巨噬细胞,构建针对小鼠TNF-α的siRNA表达框架,转染细胞,用RT-PCR方法检测TNF-αmRNA的水平,用ELISA方法检测TNF-α分泌情况。[结果]从凝胶电泳图像TNF-α/β-actin灰度值比可以看出干扰组TNF-αmRNA的水平0.18±0.03较阳性对照组0.91±0.05及阴性对照组0.85±0.10明显降低（P〈0.05）。干扰组TNF-α分泌量（21.87±1.20）pg/mL较阳性对照组（28.02±1.03）pg/mL及阴性对照组（27.64±1.92）pg/mL均明显降低（P〈0.05）。[结论]RNA干扰可以有效地抑制原代培养小鼠腹腔巨噬细胞TNF-αmRNA水平及其蛋白分泌。     

中药肝复康对实验性肝纤维化大鼠肝功能及血清标志物的影响

目的 :研究肝复康对实验性肝纤维化大鼠肝功能及血清标志物的影响。方法 :采用四氯化碳致大鼠肝纤维化模型 ,经肝复康干预后分别以赖氏法、溴甲酚绿比色法测定血清转氨酶及白蛋白、球蛋白活性 ,放免法测定血清透明质酸 (HA)、层粘连蛋白 (LN)、Ⅳ型胶原 (C -Ⅳ )、Ⅲ型前胶原 (PC -Ⅲ )。结果 :肝复康治疗后血清转氨酶、球蛋白活性降低 ,白蛋白活性升高 ,血清HA、LN、C -Ⅳ、PC -Ⅲ均有明显降低 ,与模型组比较有显著差异 (P <0 .0 1 )。结论 :肝复康对实验性肝纤维化有明确的治疗作用 ,降低肝纤维化程度 ,同时有效减轻肝细胞损伤 ,改善肝功能。     

薄膜封包对慢性皮炎小鼠模型表皮增生的影响

目的探讨薄膜封包对慢性皮炎小鼠模型表皮增生的影响。方法以0.01%佛波酯(TPA)涂搽小鼠皮肤,造慢性皮炎表皮增生模型,应用非通透性膜封包,采用组织病理及免疫组化法观察表皮增生改变及增殖细胞核抗原(PCNA)的表达。结果 TPA保鲜膜组和TPA基质组,表皮厚度(μm)分别为89.94±14.34,84.28±20.64,t=2.51;PCNA阳性标记率(%)分别为20.12±1.04,19.85±1.68,t=0.52,两者比较差异无统计学意义(P>0.05)。结论利用0.01%佛波酯(TPA)反复涂擦小鼠背部可以复制慢性皮炎模型;增殖细胞核抗原在慢性皮炎动物模型中表达明显增高;薄膜封包对慢性皮炎小鼠模型表皮增生没有明显调节作用。     

十全大补汤对荷膀胱癌小鼠大剂量化疗的减毒增效作用

Objective To investigate the toxicity attenuation and efficacy potentiation effects of Shiquan Dabu Tang (SDT) on high dose chemotherapy in T739 mice with bladder carcinoma. Methods Mouse bladder carcinoma tissue was inoculated subcutaneously into T739 mice to establish tumor-beating mice model. The tumor-bearing mice were randomly divided into a CTX group (100, 200, 400 mg/Kg respectively), a SDT group (high or low dose respectively), a high-dose SDT combined with 200 mg/Kg CTX group and a control group. The body weight, diameter of tumor nodules and complete blood count were observed subsequently. Results Different doses of SDT could effectively inhibit tumor growth in mice. SDT + CTX treatment significantly prolonged the survival time of mice by 49.4±23.3 days (P<0.01, 0.05, 0.01), compared with high dose SDT treatment (17.4±5.77) days, 200 mg/kg CTX treatment (23±14.02) days and control group (11.75±2.06) days respectively. The peripheral platelet count increased more significantly in mice treated with SDT within a week as compared to mice without SDT treatment (P<0.05). The peripheral RBC count and liB concentration increased more significantly in mice treated with SDT for 2 weeks as compared to mice without SDT treatment (P<0.05). Conclusions SDT could enhance the anti-tumor effects of high dose CTX on tumor-bearing mice and reduce toxicity in its peripheral red blood cells. The results showed that SDT combined with high dose of CTX chemotherapy had toxicity attenuation and efficacy potentiation effects in tumor-beating T739 mice.     

肝细胞琥珀酸脱氢酶活力检测条件的探讨

细胞生命活动与线粒体氧化能力的高低息息相关。线粒体的氧化能力,可通过测定其耗O_2量,或与氧化过程有关的某些酶的活性来了解。前者由于操作繁杂,容易影响测定的结果;酶活性的测定则可以选择某些与氧化还原反应有关的酶的活性来代表,琥珀酸脱氢酶(SDH)即为其一,被称为线粒体的标志酶。线粒体     

急性胰腺炎诱发当时胰、胆、胃、肠功能的变化及厚朴茵陈蒿汤的影响

急性胰腺炎极早期时胰、胆、胃、肠功能的变化尚少研究。本实验向大鼠胰管内注入2.5％去氧胆酸钠造成急性胰腺炎模型,注入后立即观察胰液分泌量及胰液淀粉酶含量,胆汁分泌量及胆汁中胆汁酸和NCO_3~-含量,胃酸分泌及胃窦峰电等。实验分三组进行:胰腺炎喂盐水组、胰腺炎喂厚朴茵陈蒿汤组及对照组。结果表明:急性胰腺炎极早期胰液、胆汁分泌及胃肠运动即迅速转向抑制,但胃酸分泌增加,这种变化可能就是临床出现胃肠内容停滞,消化功能障碍的病理基础之一,而厚朴茵陈蒿汤似有改善的作用。     

教育部本科教学评估对病理生理学教学的促进作用

教育部的本科教学评估是一项旨在提高我国高等院校教育质量,促进高等院校教学建设、教学改革和教学管理的重要举措。病理生理学教研室以本科教学评估为契机,通过大量卓有成效的工作,以期对教学的发展起到促进作用。     

十全大补汤对荷膀胱癌小鼠大剂量化疗的减毒增效作用

Objective To investigate the toxicity attenuation and efficacy potentiation effects of Shiquan Dabu Tang (SDT) on high dose chemotherapy in T739 mice with bladder carcinoma. Methods Mouse bladder carcinoma tissue was inoculated subcutaneously into T739 mice to establish tumor-beating mice model. The tumor-bearing mice were randomly divided into a CTX group (100, 200, 400 mg/Kg respectively), a SDT group (high or low dose respectively), a high-dose SDT combined with 200 mg/Kg CTX group and a control group. The body weight, diameter of tumor nodules and complete blood count were observed subsequently. Results Different doses of SDT could effectively inhibit tumor growth in mice. SDT + CTX treatment significantly prolonged the survival time of mice by 49.4±23.3 days (P<0.01, 0.05, 0.01), compared with high dose SDT treatment (17.4±5.77) days, 200 mg/kg CTX treatment (23±14.02) days and control group (11.75±2.06) days respectively. The peripheral platelet count increased more significantly in mice treated with SDT within a week as compared to mice without SDT treatment (P<0.05). The peripheral RBC count and liB concentration increased more significantly in mice treated with SDT for 2 weeks as compared to mice without SDT treatment (P<0.05). Conclusions SDT could enhance the anti-tumor effects of high dose CTX on tumor-bearing mice and reduce toxicity in its peripheral red blood cells. The results showed that SDT combined with high dose of CTX chemotherapy had toxicity attenuation and efficacy potentiation effects in tumor-beating T739 mice.