臀部局限性骨化性肌炎一例

局限性骨化性肌炎是发生在软组织的一种肿瘤样病变。常有局部外伤史,故又称外伤性骨化性肌炎,临床较少见,我们最近遇到一例报告如下: 患者女,26岁,工人。主诉左臀部肿块一月余入院。患者于一月前骑车跌倒左臀部碰撞外伤后,局部疼痛,次日臀部出现肿块     

使用缝合器处理残胃方法与胃十二指肠吻合术式的改进

我院自1989年以来，对182例BI式胃大部切除术改进了胃缝合器的使用和胃十二指肠吻合术式，未发现与残胃及吻合口有关的并发症。临床资料182例中，男137例，女45例。年龄最小为18岁，最大的68岁，平均年龄41．5岁。术前诊断胃窦癌26例（无远处转移），其他为胃窦部及十二指肠球部质高。平均手术操作时间1小时20分钟。182例均痊愈出院，无一例并发症。改进方法：在胃体于定切除线处放缝合器（保留因大弯侧4cm做为与十二指肠吻合的吻合口），拧紧，压榨缝合后，紧靠缝合器用刀切断远端胃体，移出缝合器。小弯侧残胃瑞缝合不满意或出血处全层加…     

火焰原子吸收法测定生活饮用水中铁的实验探讨

火焰原子吸收法测定生活饮用水中铁的实验探讨李永香葛少林孙莉梅周丽娟郑州市卫生防疫站（河南４５０００３）对生活饮用水中低含量（＜１．５ｍｇ／Ｌ）铁的火焰原子吸收法测定，作了研究探讨，提出了水样先经盐酸—盐酸羟胺煮沸处理定容后，再进行直接测定的方法。本法...     

122例大肠腺瘤、腺瘤癌变的粘液组化观察

大肠腺瘤是一类癌前病变,文献报道管状腺瘤、绒毛状腺瘤的癌变率分别为5％、29～70％) 本文通过对1(?)例大肠腺瘤、腺瘤癌变的粘液组化观察,探讨其在病理外检诊断中的意义,现将结果报道如下。     

中医治疗膜性肾病1例

病例患者,女性,39岁。2002年7月4日就诊。双下肢浮肿,尿中泡沫多,腰部酸痛2个月,无发热关节痛,无皮疹、脱发、光敏等,尿常规：PRO＋＋＋＋。诊断为＂肾病综合征＂,短暂服用＂泼尼松、雷公藤片＂,无缓解,自行停服。南京军区总医院解放军肾脏病研究所检查。尿检：24h尿蛋白量7.64g,     

18例宫腹腔镜联合子宫中隔切除术的护理体会

目的:总结腹腔镜监视下通过宫腔镜电切中隔子宫的手术护理经验。方法:对18例腹腔镜联合宫腔镜行子宫中隔电切手术治疗的患者给予围手术期护理。结果:本组手术均顺利,嘱患者术后工具避孕6个月,术后连用3个人工周期后取环及行宫腔镜检查,16例宫腔形态正常,1例宫底残留隔样组织1 cm,行第2次手术,1例子宫前后壁轻度粘连,宫腔镜下剪开粘连并置换一枚节育器,3个周期后复查宫腔镜,宫腔形态正常。7例术后自然妊娠,2例行体外受精联合胚胎移植术后成功妊娠,避孕3例,6例未妊娠(包括1例失诊)。结论:通过腹腔镜联合宫腔镜电切中膈子宫手术的护理认为,充分的术前准备、心理护理及术中、术后细致的观察护理,能够有效预防并发症的发生,提高临床治愈率。     

普罗布考预防对比剂肾损害的随机化临床研究

Objective Contrast induced acute kidney injury (CIAKI) is a significant clinical problem. We, therefore, performed a prospective, randomized trial to investigate the role of probueol in the prevention of CIAKI in patients with unstable angina pectoris (UAP) undergoing percutaneous coronary angiography (CAG) and interventions (PCI). Methods We studied 205 patients with UAP, who underwent CAG or PCI prospectively. Patients were randomly assigned to probucol group (n = 102) and control group (n= 103). In the probucol group, the patients received probucol tablets 500 mg b. i. d for 3 days before and after intervcntion. All the patients, after intervention, underwent hydration with intravenous saline at a rate of 1 ml per kilogram of body weight per hour for 12 hours. Results Patients were well-matched with no significant differenced at baseline in majority measured parameters between two groups. CIAKI occurred in 23 of the 205 (11.22%) patients. Multivariate logistic regression was used to identify correlates of CIAKI and clinical data. CIAKI was most strongly associated with Scr≥132.6 μmol/L (OR =21.11,95% CI 1.95-56. 06, P＜0.001), Ccr ＜60 ml/min (OR =4.19, 95%(2/1.94-9.05, P ＜0.001), heart function ＞ class Ⅱ (OR = 6.23, 95% CI 2.73-14.21, P ＜ 0.001), Diabetes (OR = 2.049, 95% CI1.19-5.25, P ＜ 0.001), age ≥ 70 yrs (OR = 3.52, 95% CI 1.66-7.43, P ＜ 0.001), coronary artery calcification shown by CAG (OR =4.29, 95% CI 1.99-9.24, P ＜ 0.001). The rate of CIAKI in probueol groups was slightly lower compared with control group (7.84% vs. 14.56%), without significant difference. The post-procedure mean peak of Scr [(101.62±42. 98) μmol/L vs. (117.67 ～68.77) μmol/L, P =0.047] and the post-procedure increasing Ser from baseline (ASer) [(13.49 ± 19. 61) μmoL/L vs.(22.50 ± 18.31) μmol/L, P =0.001] in the probucol group decreased significantly compared with that of control group. Conclusion Prophylactic treatment with probueol 500 mg b. i. d during periproeedural stage in patients with UAP has preventing role against CIAK1 after cardiac catheterization.     

围手术期患者600例抗菌药应用调查

目的：调查我院围手术期患者抗菌药物预防性应用现状,为临床合理用药提供参考。方法：采用回顾性调查分析,随机抽取我院2008年6～12月10个科室围手期住院患者病历600份,对抗茵药使用情况进行统计分析。结果：抗菌药防应用率94．0％;平均用药疗程5．3d;抗菌药合理使用率为66．8％;联合用药中二联占17．0％,三联占3．0％,四联占0．5％。结论：我院围手术期患者抗茵药使用率高,用药档次偏高,用药时间选择不当,用药疗程长,联合用药不合理。应进一步加强抗菌药的合理应用。     

增强型绿色荧光蛋白基因与hTIMP 1基因融合表达载体的构建及鉴定

目的:构建增强型绿色荧光蛋白(EGFP)基因与人组织型金属蛋白酶抑制剂1(hTIMP1)基因融合表达载体.方法:Trizol法提取总RNA,经逆转录合成cDNA.通过套式PCR获得hTIMP1开放读码框(ORF),将扩增产物与pEASY-Blunt Simple载体连接,进行序列分析.继而将该重组质粒和载体经XhoⅠ和HindⅢ双酶切,回收hTIMP1 ORF目的片段和pEGFP-C1载体大片段,将两者相连、扩增,筛选重组子进行PCR、双酶切鉴定及接头部分序列分析.结果:扩增得到hTIMP1 ORF约为624 bp,与预期长度相符.hTIMP1 ORF与pEASY-Blunt Simple载体相连,测序结果与GenBank相应序列比较,核苷酸序列无改变.hTIMP1 ORF与pEGFP-C1连接所得融合表达载体经PCR及双酶切鉴定(XhoⅠ和HindⅢ),证实hTIMP1 ORF插入pEGFP-C1.结论:成功构建EGFP基因与hTIMP1基因融合表达载体,为进一步研究hTIMP1蛋白的生物学功能和开展以hTIMP1为靶点的急性冠脉综合征的基因治疗奠定了基础.