Induced differentiation of adipose-derived stromal cells into myoblasts

This study aimed to induce the differentiation of isolated and purified adipose-derived stromal cells(ADSCs) into myoblasts,which may provide a new strategy for tissue engineering in patients with stress urinary incontinence(SUI).ADSCs,isolated and cultured ex vivo,were identified by flow cytometry and induced to differentiate into myoblasts in the presence of an induction solution consisting of DMEM supplemented with 5-azacytidine(5-aza),5% FBS,and 5% horse serum.Cellular morphology was observed under an i...     

血管内皮生长因子C表达与宫颈鳞状细胞癌局部淋巴管血管生成和浸润转移及预后的关系

Objective To examine the relation among the expression of vascular endothelial growth factor C (VEGF-C) and regional lymphangiogenesis, invasion, metastasis and prognosis in squamous cell carcinoma of cervix.Methods The expression of VEGF-C, lymphatic microvessel density(LMVD) labeled by VEGFR-3, proliferation index (PI) of cancer cells labeled by Ki-67 and microvessel density (MVD) labeled by CD34 in 28 cases of normal cervical epithelium( NCE), 36 cases of cervical intraepithelial neoplasm( CIN ) and 68 cases of squamous carcinoma of cervix (SCC)were detected by immunohistochemistry method. Results The positive rates of expressions of VEGF-C in NCE, CIN and SCC group were 21.43% , 61.11% and 72.06%, respectively. The positive expression of VEGF-C increased remarkably from NCE to CIN and SCC accordingly ( P ＜ 0. 01 ). The expression of VEGF-C in CIN significantly related to MVD ( r =0. 199, P =0. 048). The MVD in the cases with positively expressed VEGF-C in CIN was significantly higher than that with negatively VEGF-C expressed (F =4.112, P = 0. 049 ). The expression of VEGF-C in SCC significantly correlated with LMVD(r=0.288,P=0.017), but not with PI(r=0.085, P=0.489) and MVD(r=0. 181, P=0. 139). The LMVD in the cases with positively expressed VEGF-C in SCC was remarkably higher than that with negatively VEGF-C expressed ( F =6.488, P =0. 013 ). The expression of VEGF-C in SCC significantly related to pelvic lymph node metastasis, intravascular infiltration and survival rate (P ＜0.05 ), but not to patients' age, FIGO staging, histological grading, and depth of stroma infiltration (P ＞0.05 ). In the cases with VEGF-C positive expression in SCC group, the rate of pelvic lymph node metastasis and intravascular infiltration was significantly higher than that in those without the conditions mentioned above ( P ＜0.05 ). The patients' survival rate in the VEGF-C positively expressed cases in SCC group was significantly lower compared with the negative cases ( log rank test, P = 0.0357 ). Conclusion The overexpression of VEGF-C in SCC may result in rapid regional lymphangiogenesis and tumor metastasis, showing a bad prognosis.     

妊娠期肾病综合征的临床分析

目的：分析妊娠期肾病综合征（NSP）的临床特点，诊断及治疗。方法：回顾分析429例妊高征病例临床资料，将重度妊高征76例中合并肾病综合征19例（肾病组）与无合并肾病综合征57例（对照组）进行对比分析，结果：肾病组血清总蛋白、白蛋白比对照组低（P＜0．001）。肾病组24h尿蛋白，肌酐比对照组高（P＜0．001），两组尿酸水平高于正常值，肾病组腹水，胸水，脑水肿，SGA发生率高于对照组，围产儿死亡率高于对照组，结论：妊娠期肾病综合征是妊高征另一特殊类型，应在治疗妊高征基础上，综合治疗，否则将影响疗效，导致母婴并发症增加。     

基质金属蛋白酶组织抑制物-1在宫颈癌的表达与临床意义

目的 :探讨基质金属蛋白酶组织抑制物 - 1(TIMP- 1)在早期宫颈癌的表达和临床意义。方法 :采用免疫组织化学 SP法检测 75例早期宫颈浸润癌 (宫颈癌组 )、18例宫颈上皮内瘤样病变 (CIN组 )和 15例癌旁正常宫颈上皮 (对照组 )中 TIMP- 1的表达情况 ,并检测其中微血管密度 (CD34 标记 )和癌细胞增殖标记指数 (Ki- 6 7标记 )。结果 :TIMP- 1主要表达于宫颈癌细胞膜和 (或 )细胞浆 ,Ki- 6 7主要表达于癌细胞核 ,CD34 主要表达于癌巢间质血管内皮细胞。从对照组到 CIN组再到宫颈癌组 ,TIMP- 1的阳性表达率未见显著升高 (P>0 .0 5 )。 TIMP- 1在宫颈癌中表达与 FIGO分期、组织学分级、盆腔淋巴结转移和间质浸润深度有关 (P<0 .0 5 ) ;而与年龄、组织学类型、脉管浸润、Ki- 6 7表达及微血管密度无关 (P>0 .0 5 )。 FIGO分期为 期、组织学分级为 级、有盆腔淋巴结转移及突破深肌层间质浸润者 ,其 TIMP- 1阳性表达率分别显著低于 FIGO分期为 期、组织学分级在 级以内、无盆腔淋巴结转移和浸润深度在浅肌层间质以内者 (P<0 .0 5 )。结论 :宫颈癌 TIMP- 1表达降低可能在侵袭转移中起一定作用。检测宫颈癌 TIMP- 1表达对进一步了解宫颈癌生物学行为和判断预后有一定价值。     

黄褐斑临证思路

黄褐斑属中医学黧黑斑、肝斑、面尘等范畴,是一种常见的获得性色素沉着性皮肤病。其好发于面部,呈蝶翅状,轻者呈淡黄色或浅褐色,点片状散布于面颊两侧,以眼部下外侧多见;重者呈深褐色或浅黑色,似面罩般遍布于面部。近年本病发病率越来越高。现代医学对黄褐斑的发病机制目前尚不十分清楚,多认为与内分泌障碍有关。多数患者妊娠后发病或伴有月经不调、盆腔炎、或卵巢、子宫的疾患,     

妊高征患者血脂七项参数变化的探讨

目的：探讨妊高征患者血脂代谢变化的特征。方法;采用全自动生化分析仪检测９０例妊高征,３０例正常晚期妊娠妇女血清总胆固醇,甘油三酯,高密度脂蛋白胆固醇,低密度脂蛋白胆固醇,极低密度脂蛋白胆固醇,载脂蛋白Ａ１及载脂蛋白,并计算ＨＤＬ－Ｃ显著降低,ＬＤＬ－Ｃ显著升高,差异有极显著意义,ａｐｏＡｌ,ＬＤＬ－Ｃ／ＨＤＬ－Ｃ比值在中重度重高征中也有显著升高;     

TORCH感染与不良妊娠结局的关系

TORCH一词最早由Nahmias等学者。于1971年提出,它是一组病原微生物英文首字母的缩写,T指刚地弓形虫（toxoplasma,TOX）,O指其他微生物（others,如柯萨奇病毒、梅毒螺旋体、微小病毒、乙肝病毒等）,     

肿瘤的浸润、转移与uPA系统的关系

恶性肿瘤浸润、转移和基质降解有关,纤溶酶原激活系统参与基质降解,在肿瘤细胞的浸润和转移中发挥作用。本文就uPA系统与肿瘤的侵袭和转移的关系作一综述,旨在为肿瘤的防治寻找新的靶点和途径。     

基质金属蛋白酶-9及基质金属蛋白酶组织抑制物-1表达失衡与宫颈癌局部肿瘤血管生成的关系

目的 :探讨基质金属蛋白酶 - 9(MMP 9)和基质金属蛋白酶组织抑制物 - 1(TIMP 1)在宫颈癌局部肿瘤血管生成中的作用。方法 :采用免疫组织化学SP法检测 75例早期宫颈癌 (ICC)、18例宫颈上皮内瘤样病变 (CIN)和 15例癌旁正常宫颈上皮 (NCE)中MMP 9和TIMP 1的表达情况 ,并检测其中微血管密度 (MVD ,CD3 4 标记 )。结果 :从NCE组到CIN组再到ICC组 ,MMP 9的阳性表达率显著升高 (P <0 0 5 )而TIMP 1未见升高 (P >0 0 5 ) ,并且TIMP 1在ICC组的阳性表达率显著低于MMP 9(P <0 0 1)。MMP 9在ICC组中表达与MVD显著正相关 (r =0 2 87,P <0 0 5 ) ,而TIMP 1与MVD无显著相关性 (r=0 195 ,P >0 0 5 )。宫颈癌中MMP 9表达高于TIMP 1者 ,其MVD显著高于MMP 9表达低于TIMP 1者 (P <0 0 5 )。结论 :MMP 9和TIMP 1表达失衡可能在宫颈癌局部肿瘤血管生成中起重要作用 ,MMP 9表达增强而TIMP 1表达降低 ,其血管生成能力可能显著增强 ,但并非唯一决定因素。检测宫颈癌中MMP 9和TIMP 1表达对进一步了解宫颈癌局部血管生成情况有一定的应用价值。     

子宫肌瘤2029例临床病理特征分析

子宫肌瘤是女性生殖器最常见的良性肿瘤．好发于30～50岁妇女，其中40-50岁妇女发病率高达51．2％～60％，多年来大宗相关报道较少见，为探讨子宫肌瘤发病趋势和临床病理特征．本文就我院收治子宫肌瘤患者作一临床分析。