Rapid effect of stress concentration corticosterone on glutamate receptor and its subtype NMDA receptor activity in cultured hippocampal neurons

Objective: To study the rapid effect of glucocorticoids (GCs) on NMDA receptor activity in hippocampal neurons in stress and to elucidate its underlying probable membrane mechanisms. Methods: Whole-cell patch-clamp recording was used to assess the effect of stress concentration corticosterone (B) on the responses of cultured hippocampal neurons to glutamate and NMDA (N-methy-D-asparatic acid). To make clear the target of B, intracellular dialysis of B(10μmol/L)through patch pipette and extracellular application of bovine serum albumin-conjugated corticosterone (B-BSA, 10μmol/L)were carried out to observe their influence on peak amplitude of NMDA-evoked current. Results: B had a rapid, reversible and inhibitory effect on peak amplitude of GLU-or NMDA-evoked current in cultured hippoeampal neurons. Furthermore, B-BSA had the inhibitory effect on INMDA as that of B, but intraeeUularly dialyzed B had no significant effect on INMDA. Conclusion: These results suggest that under the condition of stress, GCs may rapidly, negatively regulate excitatory synaptic receptors-glutamate receptors (GluRs), especially NMDA receptor (NMDAR) in central nervous system, which is mediated by rapid membrane mechanisms, but not by classical, genomic mechanisms.     

蜱传播病原体感染所致眼部神经损害的临床分析及探讨

目的：探讨森林脑炎病毒致眼部病变的临床特征及治疗原则。方法：将19例森林脑炎病人眼部表现者，经眼部专科检查、肌电图检测及免疫荧光技术血清学测定明确诊断，给予大剂量抗病毒、抗生素药物及免疫血清治疗，血管扩张剂及神经营养剂辅助治疗。结果：19例病人在1～6个月恢复与好转，肌电图检测波值正常。结论：森林脑炎病毒主要侵害眼神经及肌肉，经上述方法治疗，效果可靠。     

成功抢救重度异烟肼中毒致心脏停跳1例体会

1临床资料 患者,男,17岁,入院31小时前自服异烟肼约100片(10g).服药后约20分钟出现意识障碍,全身抽搐.急送当地医院急救.治疗期间发生3次心跳骤停,每次持续时间约2分钟.     

简易鼻塞CPAP治疗新生儿呼吸衰竭（附25例报告）

新生儿呼吸衰竭是新生儿疾病的重要死亡原因之一 ,缺氧和二氧化碳潴留可造成多脏器损伤 ,因此临床上必须采取快速而有效的方法缓解缺氧 ,我们对 2 5例新生儿呼吸衰竭给予经鼻呼吸道持续正压通气 ( CPAP)治疗 ,取得了良好效果 ,现分析如下 :1　临床资料1 .1　一般资料　 2 5例病人均为住院的危重新生儿 ,新生儿呼吸衰竭的诊断标准根据 1 986年 9月全国新生儿学术会议 (杭州 )拟定新生儿呼吸衰竭诊断标准的初步方案[1] ,其中 1 0例经血气分析符合 型呼吸衰竭 ,Pa CO2 <70 mm Hg,且呼吸节律规则 ,呼吸频率在 40～ 80次 /min,除外中枢性呼…     

Differentiation of human epidermal stem cells into fibroblasts induced by TGF-β1 in vitro

Objective To investigate the correlation between human epidermal stem cell (hESCs) and hypertrophic scar or keloid. Methods Improved collagen Ⅳ-coated adhesion methods was used to isolate and culture the epidermal stem cells after neutral protease selectively digested the dermo-epidermal junctions. After the cells were cultured and expanded in vitro, and passage 3 hESCs were induced by different concentrations of TGF-β1 (0.1, 5.0, and 10.0 ng/ml). Morphological fea-tures and identification of these cells were meseasured by HE, Masson, immunohistochemical staining on the days 3 and 7, respectively. Results After induced by TGF-β1 for 3 and 7 days, the morpholo-gy of the epidermal stem cell (hESCs) was changed into fusiform shape, similar to fibroblasts. 70 % ofthe cell which was induced by TGF-β1 were blue stained in the cytoplasm by Masson stain, which is the distinctive method for collagen, suggesting collagen appeared or increased in the cells. The collagen concentrations in supernatants of hESCs were 0.4150±0.0014, 0.3380±0. 0020, and 0.3870±0.0020, much higher than that in control group (0.0780±0.0025) and normal skin fibro-blast group (0.15004±0.0051) (P<0.05). Immunohistochemical staining revealed that positive rates of these cells for anti-vimentin staining were more than (95.00±1.20)% in experiments and (5.70±0.20)% in control group. Conclusion The differentiantion of hESCs induced by TGF-β1 into fibro-blasts indicates that hESCs may play a role in the pathogenesis of hypetrophic scar and keloid.     

牛角角蛋白与人角质层抗原检测抗角质层抗体的比较研究(摘要)

本文以牛角角蛋白和人角质层为抗原,用生物素——亲和素酶联免疫吸附试验(BA—ELISA)检测了50名银屑病患者和32名正常人血清中抗角质层抗体(ACAS 的滴度,虽然以牛角角蛋白作抗原     

人工全髋关节置换术围手术期护理体会

目的:总结全髋关节置换术围手术期的护理体会.方法:对18例人工全髋关节置换术患者采用整体化护理模式,制定切实可行的护理计划,认真做好术前、术后护理工作及功能锻炼.结果:本组18例术后恢复快,效果满意,无1例因护理不当而引起并发症,均在2～3周出院.结论:全髋关节置换术围手术期高质量护理与手术成功密不可分.     

改良加藤厚涂片法查肠道蠕虫感染

改良加藤厚涂片法查肠道蠕虫感染刘玲刘进蔡萍以往检查肠道蠕虫卵，多采用直接涂片和饱和盐水法。前者方法简单，但由于取样量少，容易造成检出率低；后者虽可提高检出率，但由于操作复杂、繁琐，在大面积防治调查中，运用难度较大。改良加藤厚涂片法是世界卫生组织推荐的...