针灸与缺血预适应“第二保护窗”

缺血预适应作为诱发细胞自身保护反应的重要机制,对包括心肌细胞在内的各种组织器官均有明显的保护作用,尤其是缺血预适应"第二窗口"保护或延迟性保护的作用较强,时间窗较宽,并有可能通过针灸预处理等手段模拟诱导其保护作用。文章从缺血预适应的信号转导途径的"触发物质-中介物质-效应子"3个环节,总结了针灸与缺血预适应触发物质、针灸与缺血预适应中介物质以及针灸与缺血预适应延迟保护效应的研究进展。     

邯郸市中小学生贫血调查分析

为了解我市中小学生贫血患病状况，于1991年4～5月间，对我市4所城市中小学校7～18岁1313名学生进行了贫血状况的调查．1对象、方法和标准选择我市辖区内的中小学校各2所，每年级一个班的全体学生为检测对象．采用WHO推荐的氰化高铁血红蛋白法．每天上午采血，均取措血．用721分光光度计进行测定．低血红蛋白诊断标准：7～13岁Hb值低于120g／L；14岁以上男生低于130g／L；女生低于120g／L.2结果与分析2．1Hb均值：调查结果显示，男生Hb均值为131．3g／L（119.9～142.8g／L）；女生Hb均值为123．8g／L，（119.0～126．8g／L），男生高…     

艾灸温补作用的理论探源

从温补学说的形成;温补乃治虚之本;温补之法,莫过于灸法;温补中阳、脾胃之要;温、通、疏三法的灵活应用等5个方面,对艾灸温补作用的理论进行了论述和探源。希望对今后能更好的研究和探讨艾灸温补理论的临床应用提供帮助。     

隔药饼灸对功能性胃肠病肝郁脾虚模型大鼠MTL、GAS、VIP的影响

[目的]观察隔药饼灸对功能性胃肠病(Functional Gastrointestinal Disorders,FGIDs)肝郁脾虚模型大鼠胃肠激素的影响,探讨隔药饼灸治疗FGIDs的可能作用机制。[方法]将60只SD大鼠随机分为空白组、模型组、隔药饼灸组、逍遥散组和多潘立酮组5组,每组12只,除空白组外其余4组采用复合病因造模法(慢性束缚应激+过度疲劳+饮食失节)造模,连续21d;再按被试因素施加方法每天进行隔药饼灸和逍遥散、多潘立酮灌胃14d。实验结束后取血清用ELISA法检测胃动素(Motilin,MTL)、胃泌素(Gastrin,GAS)和血管活性相关肠肽(Vasoactive Intestinal Peptide,VIP)的水平。[结果]隔药饼灸组、逍遥散组与多潘立酮组大鼠MTL、GAS含量明显高于模型组,VIP含量明显低于模型组,差异均有高度统计学意义(P<0.05或P<0.01),但三组间比较差异无统计学意义(P>0.05)。[结论]隔药饼灸组能和逍遥散组、多潘立酮组一样通过升高血清中MTL和GAS水平,降低VIP的水平从而达到促进胃肠运动的作用,这可能是其作用机制之一。     

鼻敏片对变态反应性鼻炎豚鼠黏附分子的影响

目的：观察鼻敏片干预变态反应性鼻炎豚鼠黏附分子的表达及黏附分子之间的相关性,以探讨其作用机制。方法：将豚鼠按卵清蛋白致敏法制备变态反应性鼻炎模型,随机分为空白组、模型组、鼻敏片低剂量组、鼻敏片高剂量组、开瑞坦组,每组12只,给药15天．．检测豚鼠血清中细胞黏附分子-1（IcAM-1）及其配体淋巴细胞相关抗原-1（LFA-1）,以及血管细胞间黏附分子-1（VCAM-1）及其配体很晚抗原-4（VLA-4）。结果：血清ICAM-1、LFA-1、VCAM-1、VLA-4在各组中的表达比空白组高,差异均有显著性意义（P〈0．05）,血清ICAM-1、LFA—1、VCAM-1、VLA-4在各组中的表达比模型组低,差异均有显著性意义（P〈0．05）,血清ICAM-1、LFA—1、VCAM-1、VLA-4在鼻敏片低剂量组、鼻敏片高剂量组的表达与开瑞坦组比较,差异无显著性意义（P〉0．05）。血清中ICAM-1与LFA-1的含量经分析后,证实呈线性回归关系（r=0．812,F=93．078,P=0．000）。血清中VCAM-1与VLA-4的含量经分析后证实呈线性回归关系（r=0．733,F=71．491,P=0．000）。结论：鼻敏片能降低变应性鼻炎豚鼠血清中[CAM-1、LFA—l、VCAM-1、VLA-4的含量。     

自拟通腹降浊汤治疗慢性肾衰竭128例临床观察

目的：观察通腹降浊汤治疗慢性肾衰竭的疗效。方法：128例患者随机分为治疗组64例、对照组64例,均治疗2个月,判定两组的临床效果并测定治疗前后生化指标的改变。结果：治疗组64例,显效24例（37.5%）,有效19例（29.7%）,稳定13例（20.3%）,无效8例（12.5%）,总有效67.2%;对照组64例,显效12例（18.8%）,有效16例（25.0%）,稳定17例（26.6%）,无效19例（29.7%）,总有效率43.8%,两组比较有显著性差异（P〈0.01）,治疗组能明显改善尿素氮（Bun）、肌酐（Scr）、尿酸（UA）、二氧化碳（CO2）、血红蛋白（Hb）等各项指标（P〈0.05或 P〈0.01）并且优于对照组（P〈0.05或 P〈0.01）。结论：通腹降浊汤治疗慢性肾衰竭的效果明显优于对照组的治疗效果。     

严洁运用对应点针刺治疗急性扭挫伤验案举隅

急性扭挫伤是临床常见的一种疾患,可发生于任何年龄,多由剧烈运动或持重过度、跌仆等引起,无骨折、脱臼、且无皮肉破损,但症状明显,患处局部肿胀疼痛,压痛明显,或可出现红青紫等色,活动功能障碍,如不及时处置及治疗不当,会给患者造成痛苦,影响其正常生活及工作。急性扭挫伤属于中医学＂伤筋＂范畴,系因跌扑损伤导致患处经络受损,气血阻滞,壅滞局部,不通则痛。     

综合疗法治疗常年性变态反应性鼻炎30例总结

目的：探讨综合疗法治疗常年性变态反应性鼻炎的作用机理及临床疗效。方法：将90例常年性鼻炎患者随机分为3组,分别采用中药＋局部治疗＋灸疗（1组）、西药＋局部治疗＋灸疗（2组）、局部治疗＋灸疗（3组）,观察3组治疗前后症状与实验室指标变化。结果：近期疗效（治疗结束后1周内）1组总有效率为70％,2组为73％,3组为40％;中期疗效（疗程结束后4～5周内）1组总有效率为60％,2组为30％,3组为23．3％,差异显著（P〈0．05）。结论：中药配合局部治疗加灸疗的综合疗法对常年性变应性鼻炎具有较好疗效。     

谈谈一指禅推法练习技巧

一指禅推法是推拿疗法中主要手法之一。常用于骨伤科、内科、外科、妇科病症。本法的要求,最终要达到持久、有力、均匀、柔和、深透。这一要求对初学者来说非一日之功,但初学者在练习本法中普遍存在对一指禅推法的操作方法和动作要领理解、掌握困难。学生在练习中较集中的问题有两个方面:     

Effect of electroacupuncture on electrogastrogram and gastric antrum ghrelin in rats with diabetic gastroparesis

Objective

To observe the effect of electroacupuncture (EA) on the electrogastrogram and gastric antrum ghrelin in rats with diabetic gastroparesis (DGP).

Methods

Fifty Sprague-Dawley (SD) rats were randomly divided into group A, group B, group C, group D and group E, 10 rats in each group. Group A was the blank control group without intervention. Group B, Group C, Group D and Group E were treated with single dose intraperitoneal injection of 2% streptozotocin (STZ), combined with 8-week high glucose and high fat diet to establish DGP rat models. Group B was the model group without treatment. Group C was the EA at acupoint group, was treated with EA at Zusanli (ST 36), Liangmen (ST 21) and Sanyinjiao (SP 6). Group D was the EA at non-acupoint group, was treated by EA at the control points of Zusanli (ST 36), Liangmen (ST 21) and Sanyinjiao (SP 6). Rats in the metoclopramide control group received 1.7% metoclopramide solution [10 mL/(kg·bw)] by gavage. Rat’s blood glucose level was measured by blood glucose meter; gastric emptying rate was detected using phenol red as a marker; the electrogastrogram was detected by BL-420F biological function system; the protein level of ghrelin was detected by enzyme-linked immunosorbent assay (ELISA); the expression of ghrelin mRNA was detected by real-time polymerase chain reaction (RT-PCR).

Results

Compared with group A, the blood glucose of group B, C, D and E were significantly increased before and after the treatment (all P<0.01); after treatment, the gastric emptying rate of group B was significantly decreased (P<0.01), the migration rates of small intestine in group B, C, D and E were significantly lower (all P<0.01), and the protein content of ghrelin in group C was significantly decreased (P<0.01); the expressions of ghrelin mRNA were significantly increased in group B, C, D and E (all P<0.01), the mean amplitudes of electrogastrogram in group B and D were significantly decreased (both P<0.01). After treatment, compared with group B, the blood glucose of group C was significantly decreased (P<0.05), the gastric emptying rate and small intestine migration rate were significantly increased in group C and E (P<0.05, P<0.01), the small intestinal migration rate was significantly increased in group D (P<0.05), the expression of ghrelin in protein and mRNA in group C was significantly lower (P<0.01), the expression of ghrelin mRNA in group E was significantly lower (P<0.05), and the mean amplitude of electrogastrogram in group C was significantly increased (P<0.05). After treatment, compared with group D, the protein and mRNA expressions of ghrelin in group C were significantly decreased (P<0.01). After treatment, compared with group E, the protein expression of ghrelin in group C was significantly decreased (P<0.01).

Conclusion

EA at Zusanli (ST 36), Liangmen (ST 21) and Sanyinjiao (SP 6) could regulate the blood glucose level of DGP model rats, enhance electrogastrogram activity, promote gastric emptying, and regulate ghrelin expression in protein and mRNA.