六味地黄颗粒对哮喘大鼠炎症介质白三烯β4、白三烯C4及5-脂氧合酶影响的研究

目的 探讨六味地黄颗粒对哮喘大鼠炎症介质白三烯β4(LTB4)、白三烯C4(LTC4)及5-脂氧合酶(5-LO)含量的影响.方法 将SFP级SD大鼠160只随机分为8组,每组20只.除空白对照组和生理盐水组外,其余各组均以卵蛋白(OVA)辅以氢氧化铝凝胶为佐剂注射致敏,2周后雾化吸入2%OVA激发哮喘,建立哮喘模型,从实验第8天,各组分别用药干预,生理盐水组用生理盐水腹腔注射及吸入代替,空白对照组不予处理,造模时间共28d,取材后,高倍镜下观察各组大鼠肺及支气管的病理学改变;采用ELISA法检测肺组织匀浆中LTB4、LTC4的浓度.对大鼠肺组织中5-LO含量进行免疫组化评估.结果 六味地黄颗粒组、六味地黄颗粒辅助地塞米松组、六味地黄颗粒辅助布地奈德组肺组织中LTB4、LTC4的浓度明显低于哮喘模型组(P＜0.05).各用药组肺组织中5-LO含量与哮喘模型组比较并无明显差异(P＞0.05).结论 六味地黄颗粒在治疗哮喘过程中可能抑制肺组织中LTB4、LTC4 分泌,这可能是其治疗哮喘的作用靶点之一.     

小儿咳嗽变异性哮喘的中西医研究近况

从中西医发病机制,中医、西医及中西医结合治疗等几个方面总结了近年来对小儿咳嗽变异性哮喘的相关研究,展望了中西医结合治疗本病的优势和发展前景.     

含抗生素可活动关节骨水泥对全膝关节置换过程中血沉、C-反应蛋白及关节功能的影响

BACKGROUND: Antibiotic-loaded bone cement for artificial joint fixation can locally play the anti-inflammatory effects of antibiotics, thereby preventing the occurrence of infection. OBJECTIVE: To investigate the effect of antibiotic-loaded bone cement on erythrocyte sedimentation rate, C-reactive protein and joint function of patients treated by total knee arthroplasty. METHODS: Totally 86 patients, including 43 males and 43 females, aged 45-75 years, were enrolled and divided into observation and control groups (n=43/group). The artificial knee joints of patients in the observation group were fixed with antibiotic-loaded bone cement, while the artificial knee joints of patients in the in the control group were fixed with conventional bone cement. The surgical condition, blood indexes, knee function and therapeutic efficacy were compared between these two groups. RESULTS ADN CONCLUSION: The bed time and incidence of infection after operation in the observation group were significantly lower than those in the control group (P < 0.05); the knee bending angle and total efficiency of treatment after 2 weeks of treatment were significantly higher than those in the control group (P < 0.05). The C-reactive protein level and erythrocyte sedimentation rate at 3 and 7 days after treatment in these two groups were significantly decreased compared with those before treatment (P < 0.05), especially in the observation group (P < 0.05). The knee joint function scores after 6 months of treatment in these two groups were significantly increased compared with those before treatment (P < 0.05), especially in the observation group (P < 0.05). These results demonstrate that antibiotic-loaded bone cement is conductive to prevent the occurrence of infections, improve blood indexes and promote the knee joint function recovery.      

CRISPR/Cas9技术敲除斑马鱼rpl15基因对斑马鱼红系造血发育的影响

目的 利用CRISPR/Cas9技术敲除斑马鱼rpl15基因,探讨rpl15基因对斑马鱼红系造血发育的影响.方法 针对斑马鱼rpl15基因设计并制备gRNA(guide RNA),将gRNA与Cas9蛋白的混合物显微注射到单细胞期斑马鱼受精卵中,提取72 h胚胎基因组DNA,PCR扩增出目的片段,T7E1限制性酶切法检测基因打靶情况,随后进行测序分析确定编辑效果.通过O-dianisidine染色分析血红蛋白合成情况,中性粒细胞染色分析中性粒细胞生成情况,利用Real-time PCR检测p53、mdm2、hsp70和造血相关转录因子基因的表达变化.结果 成功制备了rpl15 gRNA,并筛选得到rpl15突变体.rpl15基因敲除的幼鱼出现体长缩短、尾部弯曲、头部和眼睛较小及心包水肿的表型;造血相关谱系染色结果显示rpl15突变体血红蛋白合成明显减少,但中性粒细胞生成未受影响;rpl15突变体p53和mdm2的mRNA表达均显著升高(P＜0.01,P＜0.05,),α-globin、gatal和hsp70的mRNA表达均显著下调(P＜0.01,P＜0.05,P＜0.05),除红系外的造血相关转录因子的mRNA表达与对照组相比均无明显差异.结论 利用CRISPR/Cas9技术成功编辑了斑马鱼rpl15基因,突变体产生了类似先天性纯红细胞再生障碍性贫血疾病的表型.同时表明p53与hsp70可能参与调控突变体表型的形成.     

特发性肺纤维化中上皮间质转化的生物力学特性EI北大核心

特发性肺纤维化(IPF)是一种进行性瘢痕形成的疾病,死亡率高,已引起广泛关注。上皮间质转化(EMT)是肺纤维化进程中重要的一环,而肺组织生物力学特性的变化对其有重要影响。本文总结了近年IPF-EMT中肺组织生物力学微环境的变化规律,并对肺纤维化中力学微环境的改变对EMT进程的影响、力学因素对EMT肺泡上皮细胞行为的影响和EMT中生物力学信号的传导进行了系统综述,以期为IPF的防治研究提供新的参考。