新生儿窒息后血糖,血气,心肌酶活性测定及其相关因素分析

目的：探讨新生儿窒息后血糖，血气，心肌酶（谷草转氨酶，磷酸肌酸激酶及乳酸氢酶）的变化及其相关性。方法；血糖采用微量血糖仪测定，血气用足跟动脉化毛细血管法，心肌酶采用酶偶联连续监测，相关分析采用多元线性回归法。结果：低血糖８例，高血糖３例；３６例均存在着不同程度的低氧血症和混合性酸中毒；２２例心肌酶活性增高。血糖值与日龄呈负相关，与体温呈正相关，心肌酶活性男性高于女性，各酶之间有一定的相关性，但并非     

北宋官刻雕板医书浅谈

在中国古代医学文献史上，北宋雕板医书是个极其重要而特殊的阶段，其成就首推官刻。北宋10次中央官刻医书以其丰富的内容使宋以前古代医籍第一次得到真正的保存、传播和发展，并创造使用了新的雕板标识方法和技术，统一或规范了部分经典医籍的刊刻形式与版本体例。已成为今天了解古医籍成书源流、判别版本优劣、辨析文字真伪和流派承传、审定史料学术价值的重要手段和依据。     

谷氨酸诱导体外培养的鸡胚脊髓神经细胞释放NO

用鸡胚脊髓神经细胞的原代培养,测定细胞中亚硝酸盐的含量,研究了谷氨酸(Glu)对原代培养神经细胞中NO的影响。结果表明,谷氨酸作用于原代培养的鸡胚脊髓神经细胞,在诱导神经细胞释放乳酸脱氢酶(LDH)的同时,还可诱导细胞释放一氧化氮(NO)。如先用NO合成酶抑制剂L-NOARG作用细胞,再加入Glu,则发现L-NOARG能降低Glu导致的培养液中LDH活性升高。提示NO可能参与介导Glu的神经毒性作用。     

血管内支架模型及离体血管经腔内超声三维重建的实验研究

我们自制了16个不同形状和大小的金属支架模型，置入人造血管腔中，并采用高体正常血管8条（犬腹主动脉4条，人冠状动脉4条）进行经血管腔内超声显像（IVUS）的体元模型三维重建（3DR）研究。结果显示，该技术能真实地再现支架在管腔内的形态、支架与管壁之间的间隙及离体正常血管的管腔及管壁形态，经提取后重建的支架模型与实物非常相似。经3DR测得的支架及血管腔内径与实测值均高度相关（r分别为0.96和0.99，p＜0．001）。支架与管壁之间空隙的3DR测值与实测值也高度相关（r=0.97，p＜r.0.01）。     

美沙酮联用丁丙诺啡对海洛因依赖重度药瘾戒毒治疗临床研究

目的　探索对海洛因依赖重度药瘾较理想的戒毒治疗方法。　　方法　采用美沙酮与丁丙诺啡联合用药方案 ,对海洛因依赖重度药瘾 41例行戒毒治疗 ,1 2天为一疗程 ,并与单用美沙酮组 2 0例进行比较。　　结果　联合用药组控制症状较彻底 ,鸦片类药物戒断症状量表 (OWS)总分平稳下降 ,症状波动小 ,减药顺利 ,两药替换平稳 ,戒毒成功率 73 2 %。　　结论　我们认为美沙酮联用丁丙诺啡是一种值得推荐的戒毒治疗方法。     

β—内啡肽增强人外周血单个核细胞IL—2和IFN—γmRNA的表达

应用逆转录-多聚酶链反应及Sonthern杂交技术，研究卜内啡肽（β-END）对PHA诱导的人外周血单个核细胞IL－2和IFN－ΥmRNA表达的调节作用。结果发现β-END（10-8～10-14mol／L）可显著增强IL－2和IFN－ΥmRNA的表达并呈剂量依赖性关系，此外发现β-END对IL－2mRNA的增强作用可被阿片肽受体桔抗剂——纳络酮逆转。本研究从基因水平上证明了神经递质可通过影响免疫细胞细胞因子调节免疫功能。     

生物活性材料义眼台

由于病变和外伤等原因，使一些人眼球摘除，留下外现缺陷。医学上使用义眼台植入眼眶，在上面按装义眼以矫治外形。从前使用的义眼台用玻璃球或硅橡胶制作，使用中均有种类不同的缺点，即无生物活性、密度大等。医学界希望有新型医用材料临床应用。采用液态化学方法合成羟基磷灰石，使羟基磷灰石和微晶玻璃混匀，制成生物活性材料。生物材料和粘结剂、造孔剂，辅助剂一同制成生坯，以适当的温度焙烧成多孔结构义眼台。对材料进行了动物实验与临床应用。动物实验表明，材料具有优异的生物相容性和一定的生物活性。临床应用效果良好。本材料是制备义眼台的新材料。本文就材料研究、动物实验、义眼台的生产工艺进行了探讨。     

Induction of HSP72 in Rat Liver by Chronic Ethanol Consumption Combined with Exercise: Association with the Prevention of Ethanol-Induced Fatty Liver by Exercise

Ethanol-induced fatty liver in rats was attenuated by repeated running exercise, and the protective effect of exercise was associated with the synergistic expression of heat shock proteins (HSP72). Rats were placed in four groups of six. The two ethanol-fed groups of rats received a liquid diet (Lieber-DeCarli formulation) in which 36% of the calories were derived from ethanol. One group remained sedentary (S/E), whereas the other was trained to run on a rodent treadmill at a speed of 27 m/min, 1 hr/day, 5 days/week, for 7 weeks (R/E). Two other groups–one exercised as previously mentioned (R/C) and one sedentary (S/C)–received control-liquid diets in which the ethanol was isocalorically substituted with a dextran/maltose mixture. The degree of fatty infiltration in liver sections stained with hematoxylin and eosin was graded on a 0–4 scale and the data analyzed by ANOVA on ranks. Ethanol significantly induced fatty infiltration in the S/E group, whereas fatty infiltration in the livers of the R/E group was not different from the S/C group. Electrophoresis and Western blotting of liver homogenates demonstrated that HSP72 was not expressed in either the S/C or S/E groups and was only slightly expressed in the R/C group. The combination of exercise and ethanol, however, resulted in an elevated expression of HSP72 in the R/E group. The content of HSP73 was unaffected by any treatment.     

Heat probe thermocoagulation as a substitute for surgical intervention to arrest massive peptic ulcer hemorrhage: an experience in 153 cases

In a period of 2 years 7 months, we performed heat probe (HP) thermocoagulation in 153 cases of massive peptic ulcer hemorrhage. The male/female sex ratio was 125/28. The average age was 57.6 +/- 1.3 years (mean +/- SEM; range, 17 to 88). There were 69 cases (45.1%) of spurting hemorrhage, 50 cases (32.7%) of oozing hemorrhage, and 34 cases (22.2%) of nonbleeding visible vessels. Seventy-seven patients (50.3%) were in shock before therapy. After therapy we obtained initial success in 147 cases (96.1%). Rebleeding episodes occurred in 23 patients (15.6%) within 1 month after therapy. Nineteen patients received a second therapy, and treatment in 15 of these cases (78.9%) was ultimately successful. Finally, treatment in 142 cases (92.8%) was ultimately successful. The duration of hospitalization was 6.3 +/- 0.4 days (mean +/- SEM). After discharge all patients were followed at the outpatient department for at least 1 month. Sixty-seven patients were followed endoscopically for at least 2 to 3 months after therapy. Fifty-six patients (83.6%) had a healed scar at the previous bleeding site 2 months after therapy, and 62 patients (92.5%) had a healed scar 3 months after therapy. We conclude that HP thermocoagulation is an ideal and reliable modality of therapeutic endoscopy in arrest of massive peptic ulcer hemorrhage. HP thermocoagulation may become the first choice of therapy for massive peptic ulcer bleeding in the near future.     

大肠黏膜癌变过程中PTEN和p27的表达及相关性研究

目的 :探讨PTEN及p2 7在大肠黏膜癌变过程中的表达及两者的相关性。方法 :采用免疫组织化学S -P法检测了 5 8例大肠癌 ,15例腺瘤性息肉及 11例配对的癌旁正常组织中抑癌基因PTEN和 p2 7的蛋白表达。分析PTEN和 p2 7的表达情况及与各种临床病理特征的关系及两者的相关性。结果 :在癌旁正常组织、大肠腺瘤性息肉、大肠癌组织中PTEN表达率分别为 (97.3± 4 .3) % ,(85 .2± 16 .8) % ,(13.8± 17.6 ) % ,呈递减趋势。大肠癌DukesA/B期组PTEN蛋白的高表达率为 88.9% ,明显高于DukesC/D期组中的 5 1.6 %。PTEN的高表达率与性别、年龄、肿瘤的大小、部位、分化程度、有无淋巴结转移无关。p2 7主要表达在细胞核 ,也有少量表达在细胞浆。在癌旁正常组织、大肠腺瘤性息肉、大肠癌组织中p2 7高表达率分别为 (98.8± 1.0 8) % ,(86 .0± 13.6 ) % ,(5 6 .8± 2 6 .0 ) % ,呈递减趋势。在大肠癌高、中分化组中 p2 7蛋白的表达率为 71.4 % ,明显高于低分化组中的 2 3.1%。p2 7的高表达率与患者的Dukes分期、性别、年龄、肿瘤的大小、部位及有无淋巴结转移无关。在大肠癌组织中 ,PTEN与 p2 7蛋白的表达呈正相关 (r =0 .6 4 2 )。结论 :PTEN及p2 7表达在癌旁正常组织、大肠腺瘤性息肉、大肠癌组织中均呈递减趋势 ,提示P