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101.
Yoshinori Matsuoka Yukishi Nakayama Tomoko Yamada Akira Nakagawachi Kouichi Matsumoto Kimihide Nakamura Kyousuke Sugiyama Yoshinori Tanigawa Yoshinobu Kakiuchi Yoshiro Sakaguchi 《The Brazilian journal of infectious diseases》2013,17(1):7-12
Background and aimsVibrio vulnificus causes an infectious disease that has extremely poor convalescence and leads to necrotic fasciitis. In this study, we sought to define the characteristic epidemiology of V. vulnificus infection and clarify its diagnosis at the global level.MethodsOver a period of 10 years, we investigated the appearance of symptoms, underlying conditions, treatment, and mortality in 12 patients (eight men, four women; >50 years old; average age, 66 years,) infected with V. vulnificus.ResultsThe development of symptoms occurred primarily between June and September, a period during which seawater temperature rises and the prevalence of V. vulnificus increases. All patients had underlying diseases, and seven patients reported a history of consuming fresh fish and uncooked shellfish. The patients developed sepsis and fever with sharp pain in the limbs. Limb abnormalities were observed on visual examination. All patients underwent debridement; however, in the survival group, the involved limb was amputated early in 80% patients. The mortality rate was 58.3%.ConclusionRecognition of the characteristic epidemiology and clinical features of this disease is important, and positive debridement should be performed on suspicion. When the illness reaches an advanced stage, however, amputation should be the immediate treatment of choice. 相似文献
102.
Hideki Nagashima Yasuo Morio Shunsuke Meshitsuka Koji Yamane Yoshiro Nanjo Ryota Teshima 《European spine journal》2010,19(8):1363-1368
There have been few reports describing substances related to oxidative and intermediary metabolism in the cerebrospinal fluid
(CSF) in patients with spinal degenerative disorders. This study investigated whether the concentrations of metabolites in
the CSF differed between patients with spinal degenerative disorders and controls, and whether the concentrations of these
metabolites correlated with the severity of symptoms. CSF samples were obtained from 30 patients with cervical myelopathy
(Group M), 30 patients with lumbar radiculopathy (Group R), and 10 volunteers (control). Metabolites in these CSF samples
were measured by nuclear magnetic resonance spectroscopy. There were no differences in the concentrations of lactate, alanine,
acetate, glutamate, pyruvate, or citrate between Groups M and R, between Group M and the control, or between Group R and the
control. In Group M, neither symptom duration nor the Japanese Orthopaedic Association score correlated with the concentration
of any metabolite. In Group R, the symptom duration positively correlated with the concentration of lactate, glutamate, and
citrate in CSF. The duration of nerve root block showed a negative correlation with the concentrations of acetate in CSF of
the patients in Group R. In patients with lumbar radiculopathy, there is a possibility of increased aerobic metabolic activity
or decreased gluconeogenic activity in patients with shorter symptom duration, and increased aerobic metabolic activity in
patients with severe inflammation around a nerve root. 相似文献
103.
Tatsuo Shirota Atsushi Nakamura Yoshiro Matsui Masahi Hatori Masanori Nakamura Satoru Shintani 《Clinical oral implants research》2009,20(12):1402-1408
Objective: We describe a patient who developed bisphosphonate (BP)-related osteonecrosis of the jaw (ONJ) around implants in the upper molar area.
Patients and methods: The patient was a 54-year-old woman with ulceration of the gum, bone exposure, and severe spontaneous pain around implants in the upper left molar area. She had received BPs intravenously for 2 years to treat bone metastases of breast cancer. She was diagnosed with BP-related ONJ. Sequestrum including implants was resected, and hyperbaric oxygen therapy was performed. Undecalcified ground sections were prepared from the resected bone around the implants and stained with toluidine blue. For the bone around the lesion, decalcified sections were prepared, and examined by histological and immunohistological analysis.
Results: The surgical wound became completely covered with mucosal epithelia, and postoperative pain disappeared. No recurrence of ONJ was noted during a 6-month postoperative follow-up period. However, the patient died from metastatic disease. Although histopathological examination of the resected jaw bone revealed sequestrum, osseointegration of the implant was maintained. In the area around the lesion, there was no progression of bone necrosis, and reactive bone formation, fibrosis, and invasion of lymphoid cells into the marrow cavity were observed.
Conclusion: There is no effective treatment for ONJ caused by BPs, and conservative therapy based on clinicians' experience is recommended. However, if chemotherapy is planned, or if bone necrosis around implants is thought to harbor infection, the option of jaw resection should be considered. 相似文献
Patients and methods: The patient was a 54-year-old woman with ulceration of the gum, bone exposure, and severe spontaneous pain around implants in the upper left molar area. She had received BPs intravenously for 2 years to treat bone metastases of breast cancer. She was diagnosed with BP-related ONJ. Sequestrum including implants was resected, and hyperbaric oxygen therapy was performed. Undecalcified ground sections were prepared from the resected bone around the implants and stained with toluidine blue. For the bone around the lesion, decalcified sections were prepared, and examined by histological and immunohistological analysis.
Results: The surgical wound became completely covered with mucosal epithelia, and postoperative pain disappeared. No recurrence of ONJ was noted during a 6-month postoperative follow-up period. However, the patient died from metastatic disease. Although histopathological examination of the resected jaw bone revealed sequestrum, osseointegration of the implant was maintained. In the area around the lesion, there was no progression of bone necrosis, and reactive bone formation, fibrosis, and invasion of lymphoid cells into the marrow cavity were observed.
Conclusion: There is no effective treatment for ONJ caused by BPs, and conservative therapy based on clinicians' experience is recommended. However, if chemotherapy is planned, or if bone necrosis around implants is thought to harbor infection, the option of jaw resection should be considered. 相似文献
104.
Takehiro Kukitsu Tetsuji Takayama Koji Miyanishi Atsushi Nobuoka Shinichi Katsuki Yasushi Sato Rishu Takimoto Takuya Matsunaga Junji Kato Tomoko Sonoda Sumio Sakamaki Yoshiro Niitsu 《Clinical cancer research》2008,14(1):48-54
PURPOSE: Long-standing ulcerative colitis (UC) predisposes patients to the development of colorectal cancer, but surveillance of colitis-associated cancer by detecting the precancerous lesion dysplasia is often difficult because of its rare occurrence and normal-looking appearance. In sporadic colorectal cancer, aberrant crypt foci (ACF) have been reported by many investigators to be precursor lesions of the adenoma-carcinoma sequence. In the present study, we analyzed the genetic background of ACF to determine whether they could be precursors for dysplasia, and we examined the usefulness of endoscopic examination of ACF as a surrogate marker for surveillance of colitis-associated cancer. EXPERIMENTAL DESIGN: ACF were examined in 28 UC patients (19 patients with UC alone and 9 patients with UC and dysplasia; 2 of those patients with dysplasia also had cancer) using magnifying endoscopy. K-ras, APC, and p53 mutations were analyzed by two-step PCR RFLP, in vitro--synthesized protein assay, and single-strand conformation polymorphism, respectively. Methylation of p16 was analyzed by methylation-specific PCR. RESULTS: ACF that appeared distinct endoscopically and histologically were identified in 27 out of 28 UC patients. They were negative for K-ras, APC, and p53 mutations but were frequently positive for p16 methylation (8 of 11; 73%). In dysplasia, K-ras and APC mutations were negative but p53 mutation (3 of 5; 60%) and p16 methylation (3 of 5; 60%) were positive. There was a significant stepwise increase in the number of ACF from patients with UC alone to patients with dysplasia and to patients with cancer. Univariate and multivariate analyses showed significant correlations between ACF and dysplasia. CONCLUSIONS: We have disclosed an ACF-dysplasia-cancer sequence in colitis-associated carcinogenesis similar to the ACF-adenoma-carcinoma sequence in sporadic colon carcinogenesis. This study suggests the use of ACF instead of dysplasia for the surveillance of colitis cancer and warrants further evaluation of ACF as a surveillance marker in large-scale studies. 相似文献
105.
Diagnosis of Atlantoaxial Subluxation in Morquio's Syndrome and Spondyloepiphyseal Dysplasia Congenita 总被引:1,自引:0,他引:1
Eiji Takeda M.D. Toshiaki Hashimoto M.D. Masanobu Tayama M.D. Masahito Miyazaki M.D. Etsuhisa Shirakawa M.D. Yoshiro Shiino M.D. Takahiko Saijo M.D. Michinori Ito M.D. Etsuo Naito M.D. A.H.M. Mahbubul Huq M.D. Yasuhiro Kuroda M.D. 《Pediatrics international》1991,33(5):633-638
Compression of the spinal cord due to atlantoaxial subluxation was diagnosed in a patient with Morquio's syndrome and in another with spondyloepiphyseal dysplasia (SED) congenita by cervical radiography and magnetic resonance imaging (MRI). The patient with Morquio's syndrome, a 15 year old boy, had no neurologic symptoms and his somatosensory evoked potential (SSEP) was normal. However, MRI demonstrated spinal cord compression at C1-C2. In contrast, the patient with SED congenita, an 11 year old girl, had neck pain, hyperreflexia and loss of vibration sense in both legs. These findings were explained by the absence of P3 and later waves in SSEP and by compression of the spinal cord observed on MRI. Both SSEP and MRI should be used for evaluating disorders in which atlantoaxial subluxation might be present. 相似文献
106.
Noboru Igarashi M.D. Hiroyuki Hashimoto M.D. Michio Hiratani M.D. Masashi Taniguchi M.D. Yoshiro Kasahara M.D. Tamotsu Sato M.D. Noboru Taniguchi M.D. Takashi Funabashi M.D. Norihiko Okuda M.D. Yuichiro Yamashiro M.D. 《Pediatrics international》1989,31(2):205-210
We describe the fatty acid (FA) and prostaglandin (PG) metabolism in two Japanese cases of cystic fibrosis (CF) with or without pancreatic insufficiency (PI). The diagnosis of CF was based on the elevated sweat chloride concentration by pilocarpine iontophoresis. A 1-month-old boy (case 1) showed poor weight gain, steatorrhea and scaly dermatitis, but no respiratory symptoms were noted. He had decreased levels of serum linoleate and arachido-nate, and increased palmitoleate and oleate levels, indicating essential fatty acid (EFA) deficiency. Supplementation of fat-emulsion improved his skin lesions and the altered FA pattern within a few months, associated with the definite reduction of the urinary PC Fza levels. Until two years of age, he has been free from respiratory symptoms. A 12-year-old girl (case 2) had had recurrent respiratory tract infections due to Pseudomonas ueruginoso and Staphylococcus aureus for several years, and her pancreatic functions were preserved. The FA patterns of her serum lipid were almost within the normal range. These results indicate that 1) the altered FA composition appeared to be a secondary consequence of PI commonly complicating CF and 2) the correction of the altered FA and PC metabolism might have a beneficial effect on the respiratory function of CF patients with EFA deficiency. 相似文献
107.
Hiroshi Ohtsuka Yoshiro Mafune Katsumasa Tsunashima Hideaki Takahashi Ryo Kominami 《Cancer science》1994,85(10):1015-1022
Allelic expression was examined by single-strand conformation polymorphism analysis in murinc fibrosarcomas from inter-subspecific F1 mice between C57BL/6 and MSM. Ten genes encoding p53, mdni2, E-cadherin, 72 kD metalloproteinase and its inhibitor (Timp2), thymidine kinasc and four glucose transporters (Gluts) were examined. These genes were chosen because of their probable association with tumor development and progression. In some of the tumors and cell lines, p53, E-cadherin and Glut3 genes showed remarkable differences in allelic expression, one allele being poorly expressed. The allele-specificity persisted in nine cell lines obtained by repeated transplantations from one tumor. These results suggest that expression of some genes is allele-specific in tumor cells and the pattern of specificity is stable. Such a decrease or a loss of expression in one of the alleles may be functionally equivalent to the loss of heterozygosity of the gene, and therefore this may confer malignant properties on tumor cells. It is also suggested that differential expression of two alleles is a common event in tumor cells. 相似文献
108.
Tsukio Masegi Arata Kato Kazuo Kitai Masami Fukuoka Hiroko Ogawa Yataro Ichikawa Satoshi Nakamura Naoki Watanabe Yoshiro Niitsu 《Cancer science》1995,86(1):72-80
Various novel recombinant human tumor necrosis factor-α (TNF) mutants were prepared using protein engineering techniques, and their cytotoxic activity was compared with that of the intact form of TNF (intact TNF). Mutant 471 (a TNF mutant molecule with the deletion of 7 amino acids at the amino-terminal and the substitution of Pro8 Ser9 Asp10 by ArgLysArg) had a 6-fold higher cytotoxic activity against murine L929 cells. The mutant TNF had an increased ability to bind to TNF receptor on murine L929 fibroblasts cells. A cross-linking study revealed that mutant 471 had an increased ability to form an active trimer. Mutant 471 also showed higher cytotoxic activity against human KYM myosarcoma cells and human MIA PaCa-2 pancreatic carcinoma cells. The possible cachectin activity of the mutant was almost the same as that of intact TNF. These results suggest that mutant 471 might be a more promising candidate as an anticancer agent than intact TNF. 相似文献
109.
Kazuaki Misugi Ichiro Aoki Shinzo Kikyo Yoshiro Sasaki Akio Tsunoda Takashi Nakajima 《Fetal and pediatric pathology》1985,3(2):217-226
Histological sections of 36 cases of neuroblastema and related tumors were studied with anti-S-100 protein antibody (PAP method). Schwann cells in the ganglioneuromas and ganglioneuroblastomas always strongly stained. In addition, varying numbers of spindle-shaped or elongated positively staining cells, which were probably Schwann cells and their precursor cells, were demonstrated in ganglioneuroblastoma and differentiating neuroblastoma. Undifferentiated round cell neuroblastoma showed no reaction. Immunohistochemical findings of these cases were classified into four groups (+ +, +, ±, -) according to the number of the positive cells and compared with prognosis, histological typing, location of the tumors, stage, and age at surgery. The cases with many positive cells, group (+ +) showed excellent prognosis, and group (-) showed very poor prognosis. The results of this study indicate that S-100 protein staining provides a reliable objective method for evaluation of differentiation of the neuroblastoma cells toward Schwann cells, which appears to be an important factor to predict prognosis. 相似文献
110.