自动平衡记录仪与HPLC匹配定量测定腺苷酸

本文以腺苷酸为测定物,探讨了以国产自动平衡记录仪为高效液相色谱记录并定量分析结果的方法。结果表明,从自动平衡记录上得到峰形、计算结果均与原机所带积分仪的一致。其稳定性、灵敏度和线性响应均能达到高效液相色谱检测器的要求,是一种可以代替积分仪分析结果的经济、可靠的方法。     

Pro-opiomelanocortin messenger ribonucleic acid and posttranslational processing of beta endorphin in spleen macrophages.

We have previously demonstrated low levels of immunoreactive (ir)-beta-endorphin (beta-EP) and ir-ACTH in a subpopulation of mouse spleen macrophages, which is consistent with an involvement of opioid peptides in modulation of immune responses. Gel chromatography studies suggested the presence of an approximately 3.5,000-molecular weight (mol wt) species, putatively beta-EP, an approximately 11.5,000-mol-wt species, putatively beta-lipotropin, and a higher molecular weight species (putative beta-EP precursor, pro-opiomelanocortin (POMC). In this study we have extended our original findings by demonstrating the presence of messenger RNA for POMC by the use of a complementary DNA probe and Northern blot analysis of extracts of mouse and rat spleen. In addition, using high performance liquid chromatography (HPLC), we have shown that the major endorphin species in mouse spleen macrophages is beta-EP1-31, and that there are smaller amounts of each of the acetylated forms, N-acetyl-beta-EP1-16 (alpha-endorphin), N-acetyl-beta-EP1-17 (gamma-endorphin), N-acetyl-beta-EP1-27, and N-acetyl-beta-EP1-31. We interpret these studies as showing that (a) the spleen is an organ of POMC synthesis and that (b) the predominant COOH-terminal product of macrophage POMC is the opiate-receptor active species beta-EP1-31.     

德国小蠊成虫血淋巴游离氨基酸的测定

采用离心法收集羽化后不同天数的雌雄虫血淋巴，用意大利ＣＡＢＬＯＥＲＢＡ３Ａ３ｏ型氨基酸自动分析仪测定，共检出１０种氨基酸和１个氨残基，其氨基酸总量及每种氨基酸的含量与成虫性别、不同发育期有密切的关系。     

Sry监测体外扩增造血细胞植入效果的实验研究

目的：探讨Y染色体性别决定基因(Sry)作为评价和追踪造血细胞移植后植入状态检测指标的可行性。方法：根据Sry DNA序列设计引物及制备探针，然后进行PCR检测、斑点杂交和原位杂交．动态追踪经不同条件下体外扩增的纯系雄性小鼠BMMNC回输至雌性小鼠后的植入状态。结果：PCR结果显示在各移植组小鼠的骨髓有核细胞、脾细胞及外周血白细胞中均有Y染色体特异性序列的存在；斑点杂交结果显示在各回输组间并无明显差别，但用脾脏组织作原位杂交的结果则发现基质细胞支持扩增回输组的阳性颗粒数目与输注新鲜细胞组相似，但略少于雄性小鼠；而输注细胞因子扩增细胞实验组小鼠则明显少于雄性动物的阳性对照标本和基质细胞支持扩增回输组。结论：(1)经重复检测表明上述方法的重复性好，结果可信．可作为检测性别不同时造血干细胞移植效果的一种检测手段；(2)证实基质细胞支持下的体外扩增的造血细胞具有较好的植入能力。     

Basement membranes in adenoid cystic carcinoma. An immunohistochemical study.

Tissue samples from 30 patients with adenoid cystic carcinoma and 20 with adenocarcinoma of salivary gland origin were studied by immunohistochemical staining with specific antibodies to the four macromolecules that are present in normal basement membranes: type IV collagen, laminin, heparan sulfate proteoglycan, and entactin. In the adenoid cystic carcinoma samples, the four proteins were localized in different types of extracellular matrices in the tumor, namely pseudocystic spaces, hyaline stroma, and around tumor cell nests. The staining intensity was enhanced by pretreatment with hyaluronidase. The tumor cells of adenoid cystic carcinoma showed a tendency to proliferate with individual cells in contact with the basement membrane and to infiltrate through basement membrane-rich tissues, such as peripheral nerves, blood vessels, and skeletal muscles. In contrast, only circumferential staining of tumor cell nests was obtained in adenocarcinoma samples. The results suggest that adenoid cystic carcinoma is a tumor with affinity for basement membranes, and this basic feature is reflected in its histology and presumably in its biologic behavior. Immunostaining with antibodies to basement membrane proteins appears to be useful for differential diagnosis of some types of these two carcinomas.     

九十年代中国人群的膳食与营养状况

九十年代中国人群的膳食与营养状况葛可佑，翟风英，闽怀成，程列，王青，贾凤梅（中国预防医学科学院营养与食品卫生研究所，北京１０００５０）人群的膳食营养状况在一定程度上可以反映一个国家的经济发展和社会文明程度。世界上大多数发达国家及若干发展中国家都在有计...     

检测各型乙肝患者血小板功能的临床意义

检测198例各型乙肝患者血小板功能的五个项目：血小板总数、粘附试验、聚集试验、血块退缩、血小板第3因子有效性，发现各期乙肝患者血小板功能的异常有显著性差异（P＜0．01）．并提示乙肝患者除有血小板数量的改变外，还有质量的改变，因此，全面的血小板功能检测可作为估计乙肝患者肝损害程度的辅助指标．     

Fluorescence behaviour of human arterial tissue

Total fluorescence from arterial tissue is influenced by three factors: the absorption coefficient of tissue at a specific excitation wavelength, the laser excitation power and the fluorescence coefficient which is related to chemical species in tissue. These various influences were demonstrated by the following experimental results in vitro: (1) the effect of increasing power on fluorescence intensity, (2) the total fluorescence intensity in normal aorta and plaque and (3) the effect of a chromophore such as β-carotene on total fluorescence intensity. The fluorescence intensity of normal artery is an incremental function of laser excitation power, and the fluorescence emission from normal artery compared to fluorescence emission from plaque is significantly different at the same excitation power. The total fluorescence of normal artery was measured to be twice as great as that of atheromatous plaque (relative mean ratio of 2.58±0.46 compared to unity,p<0.0002 at 488 nm; relative mean ratio of 2.57±0.51 compared to unity,p<0.0009 at 514 nm). The total fluorescence emission decreases with the increase of β-carotene content in arterial tissue (R=0.97). These emission differences, when intensified by an exogenous chromophore of β-carotene, may provide an improved guidance signal for diagnosis of plaque from normal artery during laser angioplasty procedures.     

Comparative Study on the Immunogenicity between Recombinant MS-Sj26GST Vaccine and Recombinant BCG-Sj26GST Vaccine in Schistosoma japonicum

TuberculosisandSchistosomiasisarethemajorcontagiousdiseaseswhicharethemostdangeroustothepeople’shealth Inordertogetridofthem ,wemustlookforamoreusefulvaccine Bythetech niquesofmolecularbiology ,2 6 0 0 0DaGlutathionStransferase (GST) genewasclonedintotheE coli MycobacteriumtransferringandexpressionvectorpBCG 2 0 0 0totransformittoMycobacteriumsmeg matismc2 15 5 (MS)andBCGseparatelyinordertoconstructrMS Sj2 6GSTvaccineandrBCG Sj2 6GSTvaccine Inthisstudy ,theBALB/cmicewereimmu niz…     

电针对大鼠海马兴奋性突触后电位长时程增强的作用

目的 观察电针对麻醉状态下正常和东莨菪碱引起的学习记忆减退模型大鼠海马突触EPSP长时程增强(LTP)的作用。方法 引导大鼠海马齿状回颗粒细胞层突触后兴奋性电位群(EPSPs)，强直刺激(HFS)大脑皮层前穿质区引起海马突触LTP反应；用东莨菪碱制备学习记忆障碍模型；观察电针大椎和肾俞穴对正常和模型大鼠海马LTP的影响。结果 电针对HFS诱发的海马突触LTP效应，其作用强于未电针组，部分参数和时段有统计学意义(P＜0．05)，且维持时间长于后者；东莨菪碱i．p可显著抑制HFS诱发的海马突触LTP(P＜0．01)，电针能显著对抗这一抑制作用(P＜0．01；P＜0．05)。结论 电针对HFS引起的海马突触LTP有一定的易化作用，并对东莨菪碱引起的学习记忆障碍有显著的对抗作用。