PPARγ及其配体在妇科肿瘤中的研究进展

过氧化物酶体增殖物激活受体(peroxisome prolif erator-activated receptors,PPARs)属于Ⅱ型核受体超家族成员。作为核转录调控因子受特异性配体激活后, PPARs在人体发育与代谢中起着重要的调控作用。PPARs包括α,β和γ 3个亚型,各亚型在不同的组织中分布不同,并发挥不同的生理作用。本文就γ亚型及其配体在妇科肿瘤中的研究进展作一综述。     

Chronic granulomatous disease caused by mutations other than the common GT deletion in NCF1, the gene encoding the p47phox component of the phagocyte NADPH oxidase

Chronic granulomatous disease (CGD) is an inherited immunodeficiency caused by defects in any of four genes encoding components of the leukocyte nicotinamide dinucleotide phosphate, reduced (NADPH) oxidase. One of these is the autosomal neutrophil cytosolic factor 1 (NCF1) gene encoding the p47phox protein. Most (>97%) CGD patients without p47phox (A47 degrees CGD) are homozygotes for one particular mutation in NCF1, a GT deletion in exon 2. This is due to recombination events between NCF1 and its two pseudogenes (psiNCF1) that contain this GT deletion. We have previously set up a gene-scan method to establish the ratio of NCF1 genes and pseudogenes. With this method we now found, in three CGD families patients with the normal number of two intact NCF1 genes (and four psiNCF1 genes) and in six CGD families, patients with one intact NCF1 gene (and five psiNCF1 genes). All patients lacked p47phox protein expression. These results indicate that other mutations were present in their NCF1 gene than the GT deletion. To identify these mutations, we designed PCR primers to specifically amplify the cDNA or parts of the genomic DNA from NCF1 but not from the psiNCF1 genes. We found point mutations in NCF1 in eight families. In another family, we found a 2,860-bp deletion starting in intron 2 and ending in intron 5. In six families the patients were compound heterozygotes for the GT deletion and one of these other mutations; in two families the patients had a homozygous missense mutation; and in one family the patient was a compound heterozygote for a splice defect and a nonsense mutation. Family members with either the GT deletion or one of these other mutations were identified as carriers. This knowledge was used in one of the families for prenatal diagnosis.     

Penetration depth of warm vertical Gutta-Percha pluggers: impact of apical preparation

The warm vertical gutta-percha compaction technique requires canals to be sufficiently flared to allow the tip of the plugger to penetrate to the apical third. The aim of the present study was to determine whether apical preparation or finishing instruments could be used to improve the penetration depth of pluggers. Single-root canals (48) were prepared using different rotary Ni-Ti instruments: M File System (I), ProTaper (II), HEROShaper (III), and EndoK3 (IV). Finger and heat (Endotwinn) pluggers were introduced into the canals, one after the other, and their penetration depths were measured. The apical thirds of the canals were then flared with specific instrumentation and the penetration depths of the pluggers were once again measured. After the initial preparation, the smallest diameter pluggers (No. 1 and F) could be inserted 5 to 7 mm short of working length without difficulty. Apical flaring improved the maximum penetration depth of the finger plugger by 1.76 mm and the heat plugger by 1.52 mm The deepest penetration was obtained with the EndoK3, ProTaper, and HEROShaper instruments. By accentuating the taper, apical flaring facilitated the penetration of warm vertical gutta-percha compaction pluggers.     

A one-year review of craniofacial injuries in amateur soccer players

The purpose of this study was to investigate the rate of craniofacial injuries in amateur soccer and help us better understand the nature of these injuries. Retrospective study was carried out using records from the patients with craniofacial injuries associated with soccer activities. All data were collected on the basis of sex, age, type and anatomic site of the injury. In the one-year period of this study, 11/53 cases with craniofacial injuries associated with soccer activities have been treated in our clinic. The highest incidence was in the 18 to 24 year age group (mean age 20.7) with male propensity. The majority of the patients suffered from dento-alveolar fractures (36%), followed by temporomandibular joint disorders (27%), mandibular fractures (27%), and nasal fractures (9%). The most common cause of the fractures was impact against another player (63.6%), followed by impact against equipment (18.2%) and impact against the ground (18.2%). These results show that there is a high risk of potential oral and craniofacial injury during soccer activities.     

Nonsurgical rapid maxillary expansion effects on craniofacial structures in young adult females. A bone scintigraphy study

OBJECTIVE: To evaluate the skeletal effects of nonsurgical rapid maxillary expansion (RME) on craniofacial structures with bone scintigraphy in young adult female subjects. MATERIALS AND METHODS: The material of the present study consists of scintigraphic records taken from 17 early adult females treated with RME. All patients had a bilateral posterior crossbite, transverse maxillary deficiency, deep palatal vault, and dental crowding at the beginning of the treatment. The age range of the patients was 16.1 to 18.8 years, and the mean age was 17.3 +/- 0.86 years. Bone scintigraphy records were obtained before RME (T1), during the splitting of the midpalatal suture (T2), and after the end of active widening period (T3). Repeated measure analysis of variance was used to assess the differences between the periods. In addition, Bonferroni multiple comparison tests were applied to the measurements at which F values were found to be statistically significant. RESULTS: According to the statistical analysis, significant activity changes were found in all regions studied and in all slices. The metabolic activity in all regions showed significant increases up to the separation of the midpalatal suture (T1-T2), whereas the metabolic activity exhibited a remarkable decrease (T2-T3) after the opening of the midpalatal suture. CONCLUSIONS: Scintigraphic records revealed an increase in the regions of interest scores during RME in all regions and all slices. Therefore, it can be speculated that RME has had not only dental effects but also skeletal effects on young adult patients.     

The false-positive radioiodine I-131 uptake in the foreign body granuloma located in gluteal adipose tissue

Background

The purpose of using a whole-body scanning after the radioactive I-131 treatment is to screen functional residual or metastatic thyroid tissues. In whole-body scanning of some patients, false positive radioiodine I-131 uptakes may be seen in physiological uptake regions or atypical localizations.

Case report

A 54 year-old woman underwent total thyroidectomy for papillary thyroid carcinoma. A positive appearance seen in the upper postero-lateral part of the right gluteal region was determined by a post-therapy I-131 whole body scan. The colour Doppler ultrasonography, magnetic resonance imaging features and histopathological characteristics of the excised lesion were presented. The lesion was demonstrated to be a foreign body granuloma.

Conclusions

Unexpected positive findings in the post-therapy I-131 whole body scan should be confirmed with other imaging modalities in order to avoid unnecessary treatments. In uncertain situations, the diagnosis should be established histopathologically.     

A comparison of unilevel and multilevel surgery in obstructive sleep apnea syndrome

We conducted a retrospective study of 35 adults who had undergone tongue base suspension in addition to other surgery for the treatment of obstructive sleep apnea syndrome (OSAS). Our goal was to determine the most effective surgical approach by comparing these patients' preoperative apnea-hypopnea index (AHI) with their postoperative scores at 6 months. The 35 patients were divided into three groups based on a preoperative assessment of their level of airway obstruction; the assessment included a determination of AHI and endoscopic findings during the Müller maneuver. Patients in group 1 (n = 9) exhibited unilevel obstruction, and they were treated with lateral pharyngoplasty; patients in group 2 (n = 13) also had unilevel obstruction, and they underwent Z-palatoplasty; patients in group 3 (n = 13) had multilevel obstruction, and they underwent combined surgery. Postoperatively, all three groups exhibited statistically significant improvements in AHI, but we determined that of the three approaches, multilevel surgery was significantly more effective (p < 0.001).