Non-thermal plasma suppresses bacterial colonization on skin wound and promotes wound healing in mice

The present study evaluated the effect of non-thermal plasma on skin wound healing in BalB/c mice.Two 6-mm wounds along the both sides of the spine were created on the back of each mouse(n=80) by using a punch biopsy.The mice were assigned randomly into two groups,with 40 animals in each group:a non-thermal plasma group in which the mice were treated with the non-thermal plasma;a control group in which the mice were left to heal naturally.Wound healing was evaluated on postoperative days(POD) 4,7,10 and 14(n=5 per group in each POD) by percentage of wound closure.The mice was euthanized on POD 1,4,7,10,14,21,28 and 35(n=1 in each POD).The wounds were removed,routinely fixed,paraffin-embedded,sectioned and HE-stained.A modified scoring system was used to evaluate the wounds.The results showed that acute inflammation peaked on POD 4 in non-thermal plasma group,earlier than in control group in which acute inflammation reached a peak on POD 7,and the acute inflammation scores were much lower in non-thermal group than in control group on POD 7(P<0.05).The amount of granular tissue was greater on POD 4 and 7 in non-thermal group than in control group(P<0.05).The re-epithelialization score and the neovasularization score were increased significantly in non-thermal group when compared with control group on POD 7 and 10(P<0.05 for all).The count of bacterial colonies was 103 CFU/mL on POD 4 and <20 CFU/mL on POD 7,significantly lower than that in control group(109 CFU/mL on POD 4 and >1012 CFU/mL on the POD 7)(P<0.05).It was suggested that the non-thermal plasma facilitates the wound healing by suppressing bacterial colonization.     

高效液相色谱法测定益气明目丸中葛根素的含量

目的:建立益气明目丸中葛根素含量测定的高效液相色谱法.方法:色谱柱:Hypersil ODS C18(4.6 mll×100mm,5μm);流动相:甲醇-水(22:78),检测波长:250 nm;柱温:30℃;流速:1.0ml·min-.结果:葛根素在27.58～275.8 ng(r=0.999 7,n=6)范围内呈良好的线性关系;平均回收率为98.9%;RSD为1.4%.结论:本法检测快速,定量准确,可用于益气明目丸的定量分析.     

试谈痰饮病的“温”“和”法则

目的 研究和推广仲景治疗痰饮病的法则。方法 对献资料进行分析、归类、释疑等整理,寻找其共性和个性,研究其辨证思路。结果 治疗痰饮病的“温”“和”法则 ,是原则性与灵活性有机结合;“温”要不刚不燥,“和”有标本缓急、因人而宜等含义。结论 掌握了“温”“和”的治疗原则,就能有效地治疗痰饮病症。     

Paeonol attenuates heart failure induced by transverse aortic constriction via ERK1/2 signalling

ContextPaeonol (PAE) is the main phytochemical from Cortex Moutan. Its main pharmacological effects are anti-inflammatory and antioxidant, but its cardioprotective effect is unclear.ObjectiveThe study investigates the effects and underlying mechanisms of PAE on transverse aortic constriction (TAC)-induced heart failure (HF) in mice.Materials and methodsC57BL/6 mice were randomly divided into five groups: sham, TAC, PAE10 (TAC + PAE 10 mg/kg), PAE20 (TAC + PAE 20 mg/kg) and PAE 50 (TAC + PAE 50 mg/kg). Paeonol was intragastrically administered to mice for 4 weeks. Mice were anaesthetized with pentobarbital sodium and underwent cardiac echocardiography using echocardiography system. Serum levels of atrial natriuretic peptide (ANP), tumour necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were measured by enzyme-linked immunosorbent assay (ELISA). Myocardial apoptosis was detected with terminal deoxynucleotidyl transferase-mediated dUTP nick end-labelling (TUNEL) staining. Haematoxylin–eosin (H&E) and Masson’s staining were used for histopathological evaluation. Western and quantitative real-time PCR (qRT-PCR) were performed to detect levels of apoptosis and fibrosis-related proteins.ResultsEchocardiography showed PAE improved cardiac function (LVEF: TAC, 52.3±6.8%; PAE20, 65.8±3.6%; PAE50, 71.4±2.5%) and H&E staining showed PAE alleviated myocardial injury (TAC: 1170.3 ± 134.6 μm²; PAE50: 576.0 ± 53.5 μm²). Western and qRT-PCR results showed that PAE down-regulated the levels of ANP, BNP and α-MHC. In addition, TUNEL and western results showed PAE significantly inhibited apoptosis. Masson and western results showed PAE inhibited cardiac hypertrophy. Western results showed the ERK1/2/JNK pathway could be inhibited by PAE.Discussion and conclusionsPaeonol regulates ERK1/2/JNK to improve cardiac function, which provides theoretical support for the extensive clinical treatment of HF.     

军队战时饮用水标准检验法

本标准与＜＜军队战时饮用水卫生标准＞＞相配套，目的是统一军队战时饮水卫生的检测技术方法，有检测指标２６项，参考检测指标８项，共计３４项。检测方法的基本特点是准确可靠，操作简易，试剂稳定，便于携带，符合战时饮水卫生保障的要求。     

热处理对新型牙科陶瓷可切削性及强度的影响

目的 分析不同晶化温度和晶化时间对新研制的一种牙科云母玻璃陶瓷切削性能及强度的影响,探讨其延性切削行为的可能机理及其与强度间的关系.方法 用钻孔实验的方法,以VITA MARKⅡ为对照组,测定七组不同晶化工艺处理的新型牙科云母玻璃陶瓷单位时间的钻孔深度,进行车床切削实验定性分析,同时测量三点抗弯强度.结果 该新材料在680℃晶化60min,抗弯强度可达到155MPa,可切削性能优于对照组,一定热处理条件下可在车床高速车削时形成连续带状切屑.结论 该新材料在国际上首次实现了牙科陶瓷材料的延性切削,强度已能满足临床要求.