花生四烯酸氧化产物对肾毒性肾炎肾脏血液动力学的影响

应用血栓素合成抑制剂、５－脂氧化酶抑制剂及１２－脂氧化酶抑制剂对肾毒性肾炎大鼠进行预处理，测定其肾小球滤过率、肾血流量及肾小球合成的前列腺素Ｅ２、血栓素Ｂ２、白三烯Ｂ４及１２－羟二十碳四烯酸。发现这三种抑制剂分别抑制制肾小球合成血栓素Ｂ２、白三烯Ｂ４及１２－羟二十碳四烯酸，并缓解了肾小球滤过率及肾血流量的下降程度。由此证实以上三种物质参与肾毒性肾炎中的肾脏血液动力学紊乱。     

LOS诱导的特异性抗体分泌细胞的ELISPOT法检测

目的 :动态测定卡他性莫拉氏菌 (Moraxellacatarrhalis,M .cat)脱毒脂寡糖 (dLOS)蛋白质结合疫苗诱导的抗体分泌细胞的应答状态。方法 :以M .catdLOS蛋白质结合疫苗滴鼻免疫BALB/c小鼠。应用酶联免疫斑点试验 (ELISPOT)检测免疫小鼠不同免疫诱导部位和免疫效应部位 ,包括 :鼻相关淋巴组织 (NALT)、脾脏、颈部淋巴结、鼻内容物、肺脏和派伊尔氏结的特异性抗体分泌细胞 ,并同时测定血清、鼻冲洗液、肺泡灌洗液、唾液及粪便提取液中特异性IgA、IgG和IgM的水平。结果 :M .catdLOS蛋白质结合疫苗免疫小鼠的NALT、脾脏、颈部淋巴结、鼻内容物、肺脏和派伊尔氏结中 ,均测出分泌LOS特异抗体的抗体分泌细胞 ,以鼻内容物中IgA分泌细胞的数目最多 ,其次是在NALT和肺脏中 ,这与特异性抗体测定的结果相一致。结论 :M .catdLOS蛋白质结合疫苗经滴鼻免疫 ,能刺激产生LOS特异的黏膜和全身抗体分泌细胞的应答。ELISPOT试验具有快速、灵敏、特异的优点 ,为动态分析单个抗体分泌细胞应答规律提供了新方法。     

Primary non-Hodgkin''s lymphoma and pseudolymphoma of lung: A study of 161 patients

The authors studied 161 cases of primary non-Hodgkin's lymphomas and pseudolymphomas of lung. Small lymphocytic proliferations, which they believe to be lymphomas, constituted 31.6 per cent of cases; plasmacytoid lymphocytic and small cleaved follicular center cell lymphomas (Lukes-Collins system), 22.4 and 11.8 per cent of cases, respectively; and the remaining follicular center cell lymphomas and B-immunoblastic sarcomas, 5.6 per cent of cases. Pseudolymphomas constituted 14 per cent of cases. Most patients were elderly and asymptomatic; in most cases a solitary nodule or infiltrate was observed on a chest radiograph. Radiographic evidence of effusion was found in both lymphomas and pseudolymphomas, but hilar adenopathy was restricted to lymphomas. A few peribronchial reactive germinal centers and intralesional giant cells/granulomas were seen frequently in unequivocal lymphomas, so their presence cannot be used to exclude neoplasia. A generally monomorphic cell population and invasion of bronchial cartilage or visceral pleura are suggestive of malignancy, whereas primitive cytologic appearance and invasion of lymph nodes or parietal pleura are pathognomonic of malignancy. Diffusely admixed mature lymphocytes and plasma cells with numerous reactive follicles suggest pseudolymphomas. Immunologic determination of clonality may be diagnostically definitive. Most localized lesions in lung were treated by surgical resection, whereas in cases of extensive pulmonary disease, biopsies were performed and patients were treated by chemotherapy or irradiation. Both lymphomas and pseudolymphomas recurred, most often within three years. Pseudolymphoma recurred only in lung. When distant spread of lymphoma occurred, it commonly involved extranodal sites. Only 18 of 101 patients with lymphoma died with or of tumor, and no patient with pseudolymphoma died of disease. Neither histologic subtype among the "small cell" lymphoid lymphomas nor the presence of regional node involvement was prognostically significant, but pleural effusion on the initial chest radiograph was a significant predictor of both recurrence and mortality.     

近视药物治疗研究进展

本研究复习本世纪以来 (2 0 0 0年～ 2 0 0 4年 )有关近视药物治疗问题的文献。方法是主要通过PubMed ,对“myopia”进行检索 ,同时也参考一些重要会议最新资料。     

含Triclosan和Sodium Monofluorophosphate 新型牙膏对牙菌斑量和唾液中变异链球菌数的影响

目的评价一种含0．3％Triclosan和1．1％sodium monofluorophosphate(SMFP)新型牙膏抑制牙菌斑和唾液中变异链球菌数的效果。方法对239名12-13岁学生进行为期6个月的临床检查,在首次及以后的第3个月和第6个月进行。检查内容包括牙菌斑计数和唾液中变异链球菌数的检测。结果(1)与对照组相比较,实验组3个月和6个月后的牙菌斑计数有很明显的降低,两组间有显著性差异(P分别为0．016和0．047)。(2)两组唾液中变异链球菌数在6月后均有明显降低,但无显著性差异(P=0．41)。结论含0．3％triclosan和1．1％SMFP新型牙膏能显著抑制牙菌斑的形成。     

Stromal Progenitor Cells Present Within Liposuction and Reduction Abdominoplasty Fat for Autologous Transfer to Aged Skin

BACKGROUND: Autologous fat is used for direct transfer to locally replace fat, as well as for use intradermally in the treatment of rhytids in aged skin. OBJECTIVE: To determine if the components of the autologous fat could be separated to produce a better agent for the treatment of rhytids. METHODS: Liposuction material from four patients and fat from abdominoplasty from five patients was processed by homogenization and centrifugation to separate mature lipocytes from other stromal cell populations in its associated extracellular matrix, and then to separate this from the blood and cellular debris. The cellular layer was evaluated histologically and with the immunohistochemical antibodies for CD34, SM-actin, S-100 protein, MIB-1, Bcl-2, and factor XIIIa. RESULTS: The cellular layer showed spindle cells, some small vascular structures, a small amount of mature fat, and extracellular matrix. CD34 showed diffuse staining of most spindle and endothelial cells in all sections, factor XIIIa showed only focal staining of spindle and dendritic cells, and Bcl-2 showed light to moderate staining in scattered cells within the cellular component. S-100 protein, SM-actin, and MIB-1 were negative. CONCLUSION: Processed liposuction and abdominoplasty processed fat may be ideal for the treatment of wrinkles in aged skin because it contains progenitor stromal cells that are known to be present within the fat and which may be concentrated by processing methods.     

Rorschach Inkblot Port-Wine Stain

Abstract: We present an infant born with bilaterally symmetric, anterior and posterior port-wine stains. These leslons presented a striking resemblance to Rorschach inkblots, a phenomenon not previously reported. A discussion of the case as well as a discussion of syndromes associated with port-wine stains is provided.     

Inflammatory Tinea Capitis (Kerion) Mimicking Dissecting Cellulitis

Abstract: Tinea capitis is unusual in postpubertal individuals and is frequently misdiagnosed. In cases of inflammatory disease, prompt initiation of therapy is essential to prevent scarring and permanent hair loss. Two examples are presented to illustrate principles of evaluation and treatment.