The harmonization of cardiac troponin I measurement is independent of sample time collection but is dependent on the source of calibrator

We compared the agreement of cardiac troponin I (cTnI) values between seven cTnI assay systems in clinical samples collected from 1 h up to 11 days post acute coronary syndrome (ACS), before and after correction for calibration differences between systems. Values were referenced to two sources of common calibrator, namely, a manufactured cardiac troponin material containing the ternary ICT complex, and a serum from an ACS-positive subject. In 38 samples, cTnI varied up to sevenfold between systems, the among-systems coefficient of variation ranging from 49% to 127%. After correction for method calibration differences by reference to the cTnICT material, the inter-method variation was 43% to 99% compared with 13% to 68% for the serum-based material. Differences in cTnI isoform composition between the materials, or matrix interactions for the cTnICT calibrator, might contribute to the variation in harmonization effect. Despite the improved comparability of test values by the use of the serum-based calibrator, there was a residual variation in test values between systems that was not attributable to the time of collection of the sample post ACS. We concluded that due to the heterogeneity of clinical samples, use of a pooled human serum material might better serve as a secondary reference reagent for cTnI measurement.     

异丙酚复合氯胺酮全麻诱导对血流动力学的影响

0　引言　我们观察氯胺酮对异丙酚静脉复合诱导时血流动力学的影响 ,并以芬太尼复合异丙酚全麻诱导做对照研究 .1　对象和方法1.1　对象　 30例 ASA ～ 级择期行全麻手术的患者 .男2 0例 ,女 10例 ,年龄 18～ 5 5岁 ,体质量 49～ 75 kg,随机分为两组 ,A组为异丙酚复合氯胺酮组 ,B组为异丙酚复合芬太尼组 ,每组 15例 .1.2　方法　术前 30 min im苯巴比妥钠 0 .1g,阿托品 0 .5mg. A组诱导为 iv异丙酚 2 mg· kg- 1 ,氯胺酮 1mg· kg- 1及维库溴胺 0 .1mg· kg- 1 . B组为 iv异丙酚 2 m g· kg- 1 ,芬太尼 2 μg· kg- 1 及维库溴胺 0 .1mg…     

Close mapping of the focal non-epidermolytic palmoplantar keratoderma (PPK) locus associated with oesophageal cancer (TOC)

Focal non-epidermolytic palmoplantar keratoderma (PPK or palmoplantar ectodermal dysplasia type III) is associated with oesophageal cancer in three families: two large pedigrees located in Liverpool, UK and in the midwestern American states and one smaller family from Germany. In these families, the PPK is inherited as autosomal dominant and has a late onset, usually manifesting between 7 and 8 years of age. The disease is characterised by thickening of the pressure areas of the soles, but is not restricted to the feet and also presents with oral leukokeratosis and follicular hyperkeratosis. The disease locus [previously termed the "tylosis oesophageal cancer gene' (TOC) locus] has been mapped to 17q23-qter by linkage analysis. This region is located telomeric to the keratin 16 gene, in which mutations have been identified in focal PPK families who show no increased cancer risk. We describe the close mapping of this locus to the interval between AFMb054zf9 and D17S1603 using haplotype analysis of additional Genethon markers in the region and show that although the American family is unlikely to be related to either of the other two, the UK and German pedigrees may share a common descent. This work provides a basis for positional cloning and candidate gene analysis in order to identify a gene that may be involved in familial oesophageal cancer.      

Liposome Click Membrane Permeability Assay for Identifying Permeable Peptides

Pharmaceutical Research - To develop a novel, target agnostic liposome click membrane permeability assay (LCMPA) using liposome encapsulating copper free click reagent dibenzo cyclooctyne biotin...     

Acoustic neuromas: Gd-DTPA enhancement in MR imaging

Magnetic resonance (MR) imaging examinations were performed in ten patients with 12 acoustic neuromas before and after intravenous administration of 0.1 mmol/kg body weight gadolinium-diethylenetriamine pentaacetic acid (Gd-DTPA). The degree of enhancement was greatest with the inversion recovery sequence 1,500/500/44 (repetition time [TR]/inversion time/echo delay time [TE]), followed by spin-echo (SE) 544/44 (TR/TE) sequences, then by SE 1,500/44 and SE 1,500/80 sequences. After enhancement there was a 50% reduction for measured T1 values, 33% for T2, and no significant change for proton density. There were no toxic effects on patients. Enhanced CT scans failed to demonstrate lesions in six of 12 cases. Air-CT technique improved sensitivity in four of five cases. Enhanced MR imaging added significant clinical information in two small intracanalicular tumors and in one recurrent tumor.     

Digital radiographic imaging system with multiple-slit scanning x-ray beam: preliminary report

A digital imaging system that employs a novel scanning x-ray tube, a multiple-slit assembly (MSA), and an image intensifier (II)-TV digital system is described. Use of the MSA can increase x-ray utilization by a factor of 100 over that obtained with single-slit systems. Scatter and veiling glare can be reduced substantially by synthesizing the final image from a number of multiple-slit images, resulting in improvement in contrast sensitivity. An experimental prototype system consisted of a conventional x-ray tube and an II-TV digital system used in conjunction with mechanical scanning of the MSA. Attenuation curves measured with an aluminum stepwedge showed that scatter and veiling glare were eliminated by this approach. Reconstructed images of phantoms were superior to images obtained with conventional wide-beam exposure. Physical parameters related to design of the scanning x-ray tube and a digital system for acquiring images of the chest are discussed.     

Blood pressure independent effects of nitrendipine on cardiac structure in patients after renal transplantation

Left ventricular hypertrophy is well established as a blood pressure independent cardiovascular risk factor in patients on renal replacement therapy. The effects of antihypertensive treatment on myocardial structure and function in renal transplant recipients have been so far only rarely investigated. In a double-blind, placebo-controlled study patients were randomized to the calcium channel blocker nitrendipine or placebo if the transplanted kidney had developed a stable phase. Normotensive patients received nitrendipine 2 x 5 mg daily or placebo, hypertensive patients received 2 x 10 mg up to 2 x 20 mg nitrendipine daily or placebo. To achieve adequate blood pressure control, all patients with still elevated blood pressure on study medication received antihypertensive drugs other than calcium channels blockers. Ambulatory blood pressure recording and 2D-guided M-mode echocardiography were performed at baseline and upon completion of the study. In addition, laboratory workup (including serum creatinine and lipids) was done, and serum aldosterone, plasma renin activity, plasma angiotensin II and blood glucose levels were measured in all patients at baseline and after at least 12 months of therapy. Ambulatory blood pressure was almost identical between both groups at study baseline and follow-up. In renal transplant patients on nitrendipine, posterior wall thickness (-0.10 +/- 1.77 mm) and septal wall thickness (-0.83 +/- 2.23 mm) did not change significantly from baseline. In contrast, posterior wall thickness (0.71 +/- 0.92 mm, P < 0.01) and septal wall thickness (0.97 +/- 2.20 mm, P < 0.05) increased in patients on placebo, which differed from the observed changes on nitrendipine (ANOVA: P = 0.093 and P = 0.048, respectively). Relative wall thickness, a parameter for concentric left ventricular hypertrophy, became numerically smaller on nitrendipine therapy from 0.46 +/- 0.07 to 0.44 +/- 0.09 (-0.02 +/- 0.09, NS) but increased from 0.42 +/- 0.08 to 0.48 +/- 0.08 in the placebo arm (+0.04 +/- 0.08, P < 0.02), which was also significant between the two groups (ANOVA: P = 0.036). Endocrine parameters, lipids and blood glucose were not different between the two groups. We conclude from these data that the calcium channel blocker nitrendipine exerted beneficial effects on cardiac structure in patients after renal transplantation independent of blood pressure.      

旋光活性内酯化合物的研究:(—)MALYNGOLIDE的全合成

以己二酸为起始原料,成功地合成了有手性季碳中心的内酯化合物(—)-Mialyngolide(1),其中关键的步骤采用手性相转移催化剂进行立体选择烷基化反应,此合成路线短,总收率为10.5%。