小檗碱对大鼠阴茎海绵体磷酸二酯酶5mRNA水平的影响

目的 :检测大鼠阴茎海绵体中磷酸二酯酶 5 (PDE5 )mRNA的表达 ,进而探讨小檗碱 (berberine ,Ber)的分子作用机制。　方法 :通过逆转录酶链反应 (RT PCR)技术检测大鼠阴茎海绵体中PDE5mRNA的表达。　结果 :大鼠阴茎海绵体中有PDE5A1和PDE5A2mRNA的表达 ,以PDE5A2为主要的异构酶。与内参照 β 肌动蛋白相比 ,对照组、Ber孵育 1和 3h组的PDE5A1及PDE5A2基因mRNA的相对表达量分别为 :0 .2 2± 0 .0 2 ,0 .4 1± 0 .0 1 ;0 .1 5± 0 .0 1 ,0 .34± 0 .0 2 ;0 .1 0± 0 .0 1 ,0 .1 2± 0 .0 1。与对照组相比 ,PDE5A1、PDE5A2的mRNA表达 ,在Ber孵育 1h组降低了 32 %和 1 7%,3h组降低了 5 5 %和 71 %,其中尤以应用Ber 3h后PDE5A2的mRNA减少最为明显 (n =5 ,P <0 .0 1 )。　结论 :Ber对NO cGMP信号通路的下游关键酶 (PDE5 )具有一定的调控作用 ,尤其是抑制PDE5A2的mRNA表达 ,为Ber治疗勃起功能障碍的分子机制之一。     

RELATIONSHIP BETWEEN THE CLINICAL USE OF ANTIBIOTIC AND PRODUCTION OF ESBLS

Extended -spectrum β -lactamases(ESBLs)aremainlyproducedbymembersofthefamilyEnter obacteriaceaewhichcanhydrolyzeβ -lactamantibi oticsincludingthethird - generationcephalosporinandaztreonam ,theESBLs- producingbacillishowedmedian -highresistancetoceftazidimeandaztreonamparticularly[1] .NowadaystheprevalenceofESBLs -producingstrainshavebeenreportedinmanyareasaroundtheworld[2 ] .ButthereisfewinformationabouttherelationshipbetweentheuseofantibioticandtheproductionofESBLs .Weperformedastud…     

氩激光凝固法对糖尿病性视网膜病变的疗效观察

目的 观察糖尿病性视网膜病变(DR)不同分期者接受氩激光治疗的临床效果。方法 采用科以人2000型氩激光组合激光机对170例263只患眼分别做如下处理：(1)对非增殖期糖尿病视网膜病变(NPDR)30眼行局部氩激光凝固术(光凝)；(2)对增殖前期糖尿病视网膜病变(PPDR)156眼行次全视网膜光凝；(3)对增殖期糖尿病视网膜病变(PDR)77眼行全视网膜光凝，对合并黄斑局限或弥漫性水肿者给予局灶性光凝或格栅样光凝。于光凝后3个月检查。进行视力、眼底、视网膜荧光血管造影，并与治疗前各项结果对比。结果 视力提高92眼(35％)、无变化144眼(55％)、下降27眼(10％)。眼底检查、荧光血管造影复查示：NPDR、PPDR眼视网膜微血管瘤、水肿、渗出消退，荧光渗漏消退占89％。其余11％需补充光凝。PDR眼视网膜、视盘新生血管消退占67％，33％为部分消退需补充光凝。黄斑水肿完全或部分消退占91％，光凝后出现视网膜前灶性出血占8％，玻璃体积血为1％。结论 氩激光对不同分期DR均有效。NPDR、PPDR效果好于PDR眼，后者光凝后荧光血管造影复查以及补充光凝对提高疗效非常必要。     

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Sir, Scarpioni described a patient with acute hydrothorax complicatingCAPD who was treated with repeated autologous blood instillationinto the pleural cavity together with a switch in the mode ofdialysis     

D4S1647、D6S2414基因座在中国汉族、蒙古族、藏族的遗传多态性

目的 调查D4S1647、D6S2414基因座在中国汉族、蒙古族、藏族群体中的遗传分布规律。方法 采集308份血及唾液标本应用PCR技术，扩增产物用非变性聚丙酰胺凝胶电泳分离，银染显色分析。结果 两位点各群体基因型频率分布均符合Hardy-Weinberg平衡，每一位点等位基因频率分布在各群体间无显著差异；通过对10个汉族家系的遗传模式分析，证实了两位点等位基因传递遵循孟德尔遗传规律。结论 D4S1647、D6S2414基因座在中国汉族，蒙古族，藏族群体均具有高度遗传多态性。     

131例小儿心律失常的临床分析

分析1977～1986年因各种疾病住院伴有心律失常病例131份表明:小儿心律失常的发生、病因、预后等方面与成人相比均有其特点。本组原发疾病共计22种,首位是感染中毒性心肌炎,占50.4％。发作类型以单纯性心律失常为最多见,占42.5％。全部病例采用综合治疗,大多预后良好。     

茶水中锌含量分析方法研究

目的:建立测定茶水中锌含量的方法。方法:采用锌试剂吸光光度法测定茶水中锌含量。结果:在一定条件下,锌含量在0~80μg/25 ml范围内遵守朗伯比耳定律,方法的精密度(RSD)在1.38%~3.27%之间,回收率在94%~107%之内,检测限为5.2μg/L,表观摩尔吸光系数为2.2×104L/mol.cm。结论:本方法经用于茶水样品分析,结果符合要求。     

登革病毒感染蚊虫后几个器官敏感性的比较

以浓度为4×10~8TCID_(50)/ml和4×10～5TCID_(50)/ml的病毒液经口感染,4×10~8TCID_(50)/ml和4×10~2TCID_(50)/ml病毒液经胸接种白纹伊蚊(Aedes albopictus)。结果表明1、脑和神经节感染率相同,2、神经系统对病毒敏感性较涎腺高。     

Sensitisation to Swine Leukocyte Antigens in Patients with Broadly Reactive HLA Specific Antibodies

We have previously shown that IgG HLA specific antibodies in the sera of highly sensitised patients awaiting renal transplantation can cross-react with swine leukocyte antigens (SLA). In this study we determined the frequency of patient serum IgG HLA specific antibody binding to a porcine lymphocyte panel and the likelihood of locating a cross-match negative pig donor for sensitised patients. Serum samples (n = 82) were obtained from 35 sensitised [current IgG panel reactive antibodies (PRA) > 10%] and seven nonsensitised patients awaiting renal transplantation at Addenbrooke's Hospital, Cambridge, UK. Fifty sera had IgG HLA specific PRA of 11-84%, 20 had IgG PRA of >84% and 12 had 0% PRA (negative controls). Sera were absorbed with porcine erythrocytes to remove xenoreactive natural antibodies and tested for cross-reactive IgG HLA specific antibody binding by flow cytometry against a panel of porcine lymphocytes obtained from 23 human decay accelerating factor (hDAF) transgenic pigs. A total of 1,884 cross-match combinations were tested and 369 (20%) gave a positive porcine lymphocyte cross-match. For sera from sensitised patients with IgG PRA (11-64%), only 6 of 805 (0.75%) cross-match tests were positive. In contrast, for sera from patients with high IgG PRA (>64%), 363 of 805 (45%) cross-match tests were positive (p < 0.0001). There was no difference in the frequency of positive cross-matches between patient sera with IgG PRA 65-84% and highly sensitised patient sera with IgG PRA 85-100% [156/345 (45%) vs. 207/460 (45%)]. This study demonstrates that only patient sera with broadly reactive IgG HLA specific PRA (>64%) cross-react with porcine lymphocytes. If future clinical trials of xenotransplantation are undertaken, it may be of value to select a cross-match-negative pig organ donor for such patients.