In-vitro fertilization and the ovarian hyperstimulation syndrome.

Eight patients who developed severe ovarian hyperstimulation syndrome (OHSS) were identified among 1302 patients undergoing in-vitro fertilization (IVF) over a 1 year period (prevalence of 0.6%); 63% had ultrasonically diagnosed polycystic ovaries (PCO) and 75% were undergoing their first attempt at IVF. Pretreatment with a superactive luteinizing hormone-releasing hormone (LHRH) analogue significantly increased the prevalence of severe OHSS (1.1% versus 0.2%, P less than 0.05) compared with ovarian stimulation with clomiphene citrate and human menopausal gonadotrophin (HMG). The mean serum oestradiol concentration on the day of human chorionic gonadotrophin (HCG) administration was 8200 +/- 2300 pmol/l. A mean of 19.6 +/- 6.8 follicles had been aspirated and 13.1 +/- 7.7 oocytes recovered at transvaginal ultrasound-directed oocyte recovery. All patients had an embryo transfer and luteal support in the form of HCG. The clinical pregnancy rate was 88%, multiple pregnancy rate 71% and implantation rate 63.5 +/- 41.3%. In a group of seven patients who were hospitalized for moderate OHSS during the same period, peak oestradiol levels were significantly lower than in those with severe OHSS (P less than 0.05). Of the group with moderate OHSS, 57% had PCO, the clinical pregnancy rate was 100% and multiple pregnancy rate 43%. Patients with ultrasound-diagnosed PCO have an increased risk of developing OHSS and the dose of HMG administered to them should be minimized. In patients at risk of developing OHSS, progesterone instead of HCG should be used for luteal support. Transfer of a maximum of two embryos or freezing all embryos for transfer in a subsequent cycle may reduce the likelihood of multiple pregnancy.     

The hepatitis B immunization programme in Singapore

A voluntary immunization programme to prevent perinatal transmission of hepatitis B virus (HBV) infection in Singapore was implemented on 1 October 1985 as an integral component of the national childhood immunization programme. Up to April 1988, a total of 68,845 mothers who attended government maternal and child health clinics were screened for the disease. Of these, 2432 (3.5%) were positive for hepatitis B surface antigen (HBsAg) and 904 (1.3%) for hepatitis B e antigen (HBeAg). Virtually all the babies born to carrier mothers completed the full immunization schedule; and in addition, those of HBeAg-positive mothers were given a dose of hepatitis B immunoglobulin at birth. The hepatitis B immunization programme was extended on 1 September 1987 to cover all newborns. About 90% of the 15,943 babies delivered in government institutions from September 1987 to April 1988 were immunized at birth, with the subsequent doses being administered at maternal and child health clinics at 4-6 weeks and 5 months later. More than 85% of the children given the full course of plasma-derived and yeast-derived hepatitis B vaccine from birth continued to have protective antibody to HBV two years after immunization. The programme is being closely monitored to assess the duration of immunity and the need for booster doses, while seronegative adults are also being encouraged to be vaccinated.     

围产儿与儿童胸腺的病理改变

The most outstanding pathological changes of perinatal babies and children, based on the review of the autopsy files in the 1st Affiliated Hospital of West China University of Medical Sciences, are lymphocytic depletion, and reticuloepithelial cell swelling and/or fusiform malformation in the cortex, while in the medulla apoptosis is more prominent. We suppose that these alterations are due to immaturity of cortical thymocytes, and in the diseased condition, they are easily affected by extraordinary factors, especially the influence of corticosterone inducing acute severe necrosis, so the number of lymphocytes are obviously diminished. But, in the medulla, as intact mature lymphocytes exist, physiological phenomenon such as apoptosis is rather prominent in it. While in the medulla, Hassall's corpuscles have various characteristic changes, such as cornification, calcification, fusion, cystic change and disintegration. Besides, we observed a new alteration in the thymus defined as vacuolization. All the above pathological changes reached the peak in the 28- day group; there after, they might become either worse or better according to the condition of the disease and growth of the body. However, these are still problems to be further studied separately.     

对中国药典诺氟沙星对照品在不同溶媒中的稳定性研究

目的：考察不同溶媒配制的诺氟沙星对照品溶液能否保存。方法：用紫外分光光度法分别对5℃放置的使用不同溶媒的对照品溶液在0d、10d、20d、30d、40d测定最大吸收波长、吸收值。结果；以0．4％NaOH溶液为溶媒的诺氟沙星对照液于5℃放置40d，最大吸收波长、吸收值均未发生变化（无显著性差异P＞0．05）。而以pH4.0醛酸缓冲液、pH7．4磷酸盐缓冲液为溶媒的两种对照液5℃放置40d，最大吸收波长未发生变化，但吸收值明显降低（有显著性差异P＜0．05）。结论：用0．4％NaOH溶液配制的诺氟沙星对照品溶液在适宜的条件下（5℃，避光密闭）保存，40d内是稳定的，可继续使用。而用pH4．0醋酸缓冲注和pH7．4磷酸盐缓冲液配制的诺氟沙星对照品溶液则不能保存使用，必须临用新配。     

An Analysis of Chromosome on Sterility Caused by Azoospermia or Oligospermia

ＡｎＡｎａｌｙｓｉｓｏｆＣｈｒｏｍｏｓｏｍｅｏｎＳｔｅｒｉｌｉｔｙＣａｕｓｅｄｂｙＡｚｏｏｓｐｅｒｍｉａｏｒＯｌｉｇｏｓｐｅｒｍｉａ￥ＷｕＭｅｉｈｅｎｇ；ＴａｎｇＷｉｎｇｎｕｏ．（ＡＣＴＡＡＣＡＤＥＭＩＡＥＭＥＤＩＣＩＮＡＥＮＡＮＪＩＮＧ，１９９５（...     

~（99m）Tc－新半乳糖白蛋白体外受体介导结合特性的研究

本文报道￣９９ｍＴｃ－新半乳糖白蛋白（￣９９ｍＴｃ－ＮＧＡ）与大鼠肝细胞膜进行体外结合试验。结果表明：￣９９ｍＴｃ－ＮＧＡ系与肝细胞膜上的高亲和力低容量（Ｋ＿１：８．４０８９×１０￣９Ｌ／ｍｏｌ；Ｒ＿１：１．７３５０×１０￣（－１２）ｍｏｌ／ｍｇ）和低亲和力高容量（Ｋ＿２：２．０８２７×１０￣８Ｌ／ｍｏｌ；Ｒ＿２：６．９５３０×１０￣（－１２）ｍｏｌ／ｍｇ）２个结合位点相结合，其结合表现出高特异性、可饱和性及对细胞膜蛋白浓度的线性依赖性，具备受体介导结合的基本特征。￣（９９ｍ）Ｔｃ－ＮＧＡ是一个很有希望的肝受体显像剂。     

硫酸镍对红细胞毒性研究

健康小鼠腹腔注射硫酸镍10.0,5.0,2.5mg/kg,导致外周血中红细胞增高,血红蛋白不变或降低,红细胞中点彩红细胞和碱粒红细胞占有比值明显增高,网织红细胞含量升高、提示镍对外周血红细胞有毒性损害。