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61.
Ketanserin potentiates the prejunctional inhibitory effect of 5-hydroxytryptamine on rat vas deferens 总被引:1,自引:0,他引:1
5-Hydroxytryptamine (5-HT) slightly inhibited the twitch contractions of rat vas deferens caused by single pulse field stimulation at 0.1 Hz. The inhibitory effect of 5-HT was much less in the epididymal portion than in the prostatic portion of the vas deferens. Ketanserin potentiated the prejunctional inhibitory effect of 5-HT and attenuated its stimulatory effect. This potentiation was observable only in the epididymal portion, of the vas deferens. Cyproheptadine and mianserin, but not methysergide, had essentially similar potentiating effects to those of ketanserin. These results suggest that the 5-HT receptor that mediates prejunctional inhibition is not of the 5-HT2 type, and that ketanserin acts by suppressing the 5-HT-induced stimulatory effect, which is possibly mediated by a postjunctional 5-HT2 receptor, thus unmasking the inhibitory effect of 5-HT. 相似文献
62.
M Aida H Kanaya Y Murata T Hayakawa K Horie 《Nihon University journal of oral science》1990,16(4):454-460
We investigated the effectiveness of commercially available silane coupling agents on the adhesion between the composite resin and the porcelain. As silane coupling agents, we used Cosmotech primer. Clearfil porcelain bond. Porcelain liner M, and Scotchprime. As porcelain, we used Cosmotech porcelain DA2 which was etched either with hydrofluoric acid or with phosphoric acid gel. The tensile bond strengths between composite resin and the porcelain treated with silane coupling agents were measured after one day immersion in 37 degrees C water. Among the silane coupling agents, Clearfil porcelain bond gave the highest bond strength of about 175 kgf/cm2. When any of these silane coupling agents were used, the bond strengths were increased more by hydrofluoric acid etching than by phosphoric acid etching. Especially in the case of Scotchprime treatment, hydrofluoric acid etching was remarkably effective on the increase in the bond strengths. The value in hydrofluoric acid etching was 3 times higher than that in phosphoric acid etching. From SEM observation, hydrofluoric acid etching gave greater roughness to the porcelain surface, which resulted in greater mechanical interlocking of the resin. In the case of Cosmotech primer treatment, the combination with GC bonding agent did not increase the bond strengths, while combination with Clearfil new bond did. The phosphoric acid ester monomer in Clearfil new bond, which is known as strong acid, was supposed to catalyze the activation of silane coupling agent. 相似文献
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Ikuro Takakura Kohtaro Miyazawa Takashi Kanaya Wataru Itani Kouichi Watanabe Shyuichi Ohwada Hitoshi Watanabe Tetsuya Hondo Michael T. Rose Tsuyoshi Mori Suehiro Sakaguchi Noriyuki Nishida Shigeru Katamine Takahiro Yamaguchi Hisashi Aso 《The American journal of pathology》2011,179(3):1301-1309
Transmissible spongiform encephalopathies are fatal neurodegenerative diseases. Infection by the oral route is assumed to be important, although its pathogenesis is not understood. Using prion protein (PrP) knockout mice, we investigated the sequence of events during the invasion of orally administered PrPs through the intestinal mucosa and the spread into lymphoid tissues and the peripheral nervous system. Orally administered PrPs were incorporated by intestinal epitheliocytes in the follicle-associated epithelium and villi within 1 hour. PrP-positive cells accumulated in the subfollicle region of Peyer''s patches a few hours thereafter. PrP-positive cells spread toward the mesenteric lymph nodes and spleen after the accumulation of PrPs in the Peyer''s patches. The number of PrP molecules in the mesenteric lymph nodes and spleen peaked at 2 days and 6 days after inoculation, respectively. The epitheliocytes in the follicle-associated epithelium incorporating PrPs were annexin V-positive microfold cells and PrP-positive cells in Peyer''s patches and spleen were CD11b-positive and CD14-positive macrophages. Additionally, PrP-positive cells in Peyer''s patches and spleen were detected in the vicinity of peripheral nerve fibers in the early stages of infection. These results indicate that orally delivered PrPs were incorporated by microfold cells promptly after challenge and that macrophages might act as a transporter of incorporated PrPs from the Peyer''s patches to other lymphoid tissues and the peripheral nervous system.Transmissible spongiform encephalopathies (TSEs), or prion diseases, are fatal neurodegenerative diseases that infect humans and both wild and domestic animals. They include Creutzfeldt-Jakob disease (CJD) in humans, scrapie in sheep, and bovine spongiform encephalopathy (BSE) in cattle.1 The common neuropathological features within the central nervous system (CNS) of TSEs are seen as a spongiform pathology, neuronal loss,2 glial activation,3 and the accumulation of an abnormal and protease-resistant conformer of the scrapie-associated prion proteins (PrP-res or PrPSc),4 which are closely associated with the infection.5It has been reported that variant CJD in humans is most likely to have occurred because of the transmission of BSE after the consumption of beef contaminated with the BSE agent.6 Therefore, the oral route of TSE infection is widely assumed to be important under natural conditions. Many of the infectious agents accumulate in the gut-associated lymphoid tissues (GALT) after oral infection, such as the Peyer''s patches and mesenteric lymph nodes (MLN) before spreading to the CNS.7 It is necessary for the infectious agents to cross the intestinal epithelium before they can accumulate in the GALT. In addition, there are microfold cells (M cells) within the follicle-associated epithelium (FAE) that are specialized for the transepithelial transport of macromolecules and particles.8 One in vitro study has demonstrated that M cells actively transcytose the scrapie agents into the basolateral side of the epithelium.9 However, it is still a matter of controversy as to whether M cells may be involved in the in vivo transport of the infectious agents across the intestinal epithelium. After alimentary uptake of the infectious agents, they accumulate in the GALT and the lymphoreticular systems (eg, the spleen and other peripheral lymph nodes) long before they are detected in the CNS.10 As the GALT and the lymphoreticular systems are highly innervated, they are believed to be important sites for the infectious agents to gain contact with the nervous system (ie, neuroinvasion).11 Once neuroinvasion occurs, the infectious agents reach their initial CNS target sites by spreading in a retrograde direction along efferent nerve fibers.12In the lymphoid tissues, it is believed that the macrophages, dendritic cells (DCs), and follicular dendritic cells (FDCs) are involved in the transportation and replication of the infectious agents. Macrophages are prevalent candidates for both spread13 and clearance14 of the infectious agents. DCs can capture and retain protein antigens in a nondegraded state.15,16 These characteristics suggest that the macrophages and DCs may act as a transporter of the infectious agents from the gut to lymphoid tissues. FDCs express high levels of cellular PrPs (PrPc), and therefore an early accumulation of PrPSc is seen in them.17,18 Many studies of the alimentary pathogenesis of TSEs have been conducted to elucidate how infectious agents spread from the GALT to the CNS, although this has not been clearly determined yet. Therefore, the aim of the present study was to reveal the cells involved in the early stages of the pathogenesis of oral TSE infection, such as the sites of entry, spread, and neuroinvasion. 相似文献
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67.
Kenji Ishihara Shinichi Hiromatsu Yusuke Shintani Kurando Kanaya Keita Mikasa Shigeaki Aoyagi 《Surgery today》2009,39(9):764-769
Purpose
To evaluate the recent clinical experience with nonpermanent inferior vena cava (IVC) filter placement preoperatively, especially with regard to patients who had deep venous thrombosis (DVT) due to compression by a tumor or an aneurysm. 相似文献68.
Toshihiko Shinohara Ichiro Uyama Seiichiro Kanaya Kazuki Inaba Jun Isogaki Akihiko Horiguchi Shuichi Miyakawa 《Langenbeck's archives of surgery / Deutsche Gesellschaft fur Chirurgie》2009,394(4):733-737
Background In patients having locally advanced cancer of the stomach with suspected tumor infiltration to the pancreatic head or the
duodenum, a concurrent pancreaticoduodenectomy with gastrectomy is occasionally prerequisite to achieve a microscopically
tumor-free surgical margin.
Materials and methods We present the first series of successful totally laparoscopic pancreaticoduodenectomy (TLPD) for advanced gastric cancer
with suspected infiltration to the pancreatic head.
Results TLPD was successfully performed without adverse events during surgery and resulted in favorable short-term outcomes of three
patients with locally advanced gastric cancer with suspected invasion to the pancreas.
Conclusions Although TLPD for locally advanced gastric cancer is a technically difficult challenging operation that requires careful dissection
along the major vessels, intracorporeal tie sutures, and the placement of an external drainage tube into a narrow pancreatic
duct, this procedure is technically feasible and safe in the hands of experienced surgeons. Long-term follow-up is mandatory
to validate oncological outcome. 相似文献
69.
Combination therapy with oral sildenafil and beraprost for pulmonary arterial hypertension associated with CREST syndrome 总被引:2,自引:0,他引:2
Miwa K Matsubara T Uno Y Yasuda T Sakata K Tsuda T Kanaya H 《International heart journal》2007,48(3):417-422
Pulmonary arterial hypertension (PAH) is commonly associated with CREST (Calcinosis, Raynaud phenomenon, Esophageal motility disorders, Sclerodactyly, and Telangiectasia) syndrome. Sildenafil, an oral phosphodiesterase type-5 inhibitor, may offer benefits in the pharmacological management of PAH. However, little is known about the long-term hemodynamic effects of sildenafil, and the potential role of sildenafil in long-term combination with beraprost, an oral prostacyclin analogue, remains unclear. We therefore examined the hemodynamic effect of oral sildenafil alone and when coadministered with beraprost in a patient with PAH associated with CREST syndrome. Traces of the acute hemodynamic effects of beraprost (20 microg) disappeared after 2 hours. In contrast, the acute hemodynamic effects of sildenafil (50 mg) produced a greater reduction in PAP (31%) and PVR (40%), and these effects also disappeared after 5 hours. After 1 month of combination therapy of sildenafil (25 mg) twice daily and beraprost (20 microg) 3 times daily, the fall in pulmonary artery pressure and pulmonary vascular resistance was sustained (31% in both). Furthermore, the patient had significantly improved her 3-minute walk test and NYHA function class without significant adverse effects at the reported doses. The findings indicate that oral sildenafil is a potent pulmonary vasodilator that appears to act synergistically with oral beraprost to cause sustained pulmonary vasodilatation in a patient with PAH associated with CREST syndrome. 相似文献
70.