全文获取类型
收费全文 | 1367篇 |
免费 | 73篇 |
国内免费 | 2篇 |
专业分类
耳鼻咽喉 | 2篇 |
儿科学 | 127篇 |
妇产科学 | 8篇 |
基础医学 | 187篇 |
口腔科学 | 84篇 |
临床医学 | 65篇 |
内科学 | 419篇 |
皮肤病学 | 12篇 |
神经病学 | 69篇 |
特种医学 | 32篇 |
外科学 | 232篇 |
综合类 | 11篇 |
预防医学 | 30篇 |
眼科学 | 11篇 |
药学 | 57篇 |
肿瘤学 | 96篇 |
出版年
2023年 | 9篇 |
2022年 | 17篇 |
2021年 | 28篇 |
2020年 | 15篇 |
2019年 | 19篇 |
2018年 | 37篇 |
2017年 | 17篇 |
2016年 | 21篇 |
2015年 | 29篇 |
2014年 | 36篇 |
2013年 | 42篇 |
2012年 | 78篇 |
2011年 | 71篇 |
2010年 | 39篇 |
2009年 | 51篇 |
2008年 | 70篇 |
2007年 | 64篇 |
2006年 | 58篇 |
2005年 | 81篇 |
2004年 | 71篇 |
2003年 | 73篇 |
2002年 | 66篇 |
2001年 | 61篇 |
2000年 | 43篇 |
1999年 | 42篇 |
1998年 | 16篇 |
1997年 | 14篇 |
1996年 | 21篇 |
1995年 | 10篇 |
1994年 | 13篇 |
1993年 | 12篇 |
1992年 | 14篇 |
1991年 | 27篇 |
1990年 | 19篇 |
1989年 | 13篇 |
1988年 | 19篇 |
1987年 | 14篇 |
1986年 | 13篇 |
1985年 | 19篇 |
1984年 | 9篇 |
1983年 | 6篇 |
1981年 | 4篇 |
1979年 | 10篇 |
1978年 | 7篇 |
1975年 | 4篇 |
1974年 | 4篇 |
1971年 | 3篇 |
1969年 | 4篇 |
1968年 | 4篇 |
1965年 | 4篇 |
排序方式: 共有1442条查询结果,搜索用时 0 毫秒
31.
32.
Shouichi Ohga Eriko Higashi Akihiko Nomura Akinobu Matsuzaki Akira Hirono Shiro Miwa Hisaichi Fujii† Kohji Ueda 《British journal of haematology》1995,89(2):421-423
Summary. We report the case of a 2-year-old Japanese boy with acute favism who was treated with human haptoglobin products. He had been exhibiting chronic nonspherocytic haemolytic anaemia until the diagnosis of glucose-6-phosphate dehydrogenase (G6PD) deficiency when 14 months old. He suffered a favic crisis at 24 months of age, when the administration of haptoglobin was effective for relieving bilirubinaemia and haemoglobinuria. Serum-free Hb rapidly decreased to normal levels despite the sustained level of serum lactate dehydrogenase. His G6PD gene was G6, Guadalajara. This is the first application of haptoglobin therapy for acute favism and the first reported case of Japanese G6PD deficiency with typical favic crisis. Haptoglobin treatment might be helpful for managing the haemolytic crisis in the disease. 相似文献
33.
34.
Honda K Kanegane H Eguchi M Kimura H Morishima T Masaki K Tosato G Miyawaki T Ishii E 《American journal of hematology》2000,64(2):128-132
The X-linked lymphoproliferative disease (XLP) is an inherited immunodeficiency characterized by an abnormal responses to infection with Epstein-Barr virus (EBV), resulting in fatal infectious mononucleosis, hypogammaglobulinemia, virus-associated hemophagocytic syndrome, and malignant lymphoma. Mutations in the gene coding for a T cell-specific SLAM-associated protein (SAP) have been recently identified in XLP patients. We report on a 1-year-old boy representing fulminant hemophagocytic syndrome. He developed high fever, lymphadenopathy, hepatosplenomegaly with liver dysfunction, and pancytopenia with marrow hemophagocytosis. EBV DNA was abnormally increased in the blood. Polymerase chain reaction failed to amplify SAP mRNA and genomic DNA products from the patient' As peripheral blood. A large deletion of the SAP gene was confirmed by fluorescence in situ hybridization (FISH). FISH analysis also disclosed that the patient's mother was a carrier. We conclude that FISH can be useful in the diagnosis of XLP with large deletions of the SAP gene and its carrier state. 相似文献
35.
Ohga S Kubo E Nomura A Takada H Suga N Ishii E Suminoe A Inamitsu T Matsuzaki A Kasuga N Hara T 《International journal of hematology》2001,73(3):323-326
Epstein-Barr virus (EBV)-DNA was quantitatively measured to assess posttransplantation virus reactivation by real-time polymerase chain reaction (PCR). In the first retrospective analysis of a 7-year-old boy with lymphoproliferative disease (LPD) after an unrelated cord blood transplantation, serum EBV-DNA progressively increased to 4 x 10(5) copies/mL. EBV load was then prospectively monitored in peripheral blood from posttransplantation patients. The second case was an 8 year-old boy with aplastic anemia who received a CD34+ cell transplantation. This patient died of LPD with the progression of pulmonary nodules. EBV-DNA increased to 4 x 10(4) copies/mL after the control of cytomegalovirus reactivation. On the other hand, EBV-DNA was undetectable (<200 copies/mL) in the series of all 58 samples from 10 patients who did not develop LPD after hematopoietic stem cell transplantation. Sequential monitoring of circulating EBV-DNA by quantitative PCR may be a useful indicator for predicting the development of posttransplantation LPD. 相似文献
36.
Takahashi-Yasuno A Masuzaki H Miyawaki T Matsuoka N Ogawa Y Hayashi T Hosoda K Yoshimasa Y Inoue G Nakao K 《Metabolism: clinical and experimental》2004,53(5):650-654
Leptin and its receptors are known to play a role in glucose metabolism. We succeeded in cloning human Ob-R cDNA and revealed 7 single nucleotide polymorphisms (SNPs) (Lys109Arg, Arg223Gln, Ser343Ser, Ser492Thr, Lys656Asn, Ala976Asp, and Pro1019Pro) in the coding region of Ob-Rb. Although these 7 SNPs were not associated with an obese phenotype, several studies have reported that some of them were associated with impaired glucose metabolism. To clarify whether the Arg223Gln and A3057G (Pro1019Pro) polymorphisms influence glucose metabolism in Japanese, 696 Japanese men were genotyped. Individually, the Arg223Gln and the A3057G polymorphisms were not associated with the glucose metabolic parameters. No associations were found between haplotype and clinical parameters. However, in 327 subjects with normal glucose tolerance (NGT), the subjects with Arg/Gln or Gln/Gln + A/A haplotype showed significantly higher serum insulin levels and homeostasis model assessment (HOMA) index than those with Arg/Arg + A/A haplotype and Arg/Gln or Gln/Gln + A/G or G/G haplotype. The subjects with Arg/Gln or Gln/Gln + A/A haplotype showed a significantly lower fasting glucose to insulin (GI) ratio than those with Arg/Arg + A/A haplotype. These results suggest that the Ob-R gene may serve as a modifier gene for insulin resistance in Japanese men. 相似文献
37.
38.
39.
Levels of interleukin-18 and its binding inhibitors in the blood circulation of patients with adult-onset Still's disease 总被引:14,自引:0,他引:14
Kawashima M Yamamura M Taniai M Yamauchi H Tanimoto T Kurimoto M Miyawaki S Amano T Takeuchi T Makino H 《Arthritis and rheumatism》2001,44(3):550-560
OBJECTIVE: Interleukin-18 (IL-18) is a proinflammatory cytokine that is involved in immunologically mediated tissue damage, but its bioactivity is regulated in vivo by its soluble decoy receptor, IL-18 binding protein (IL-18BP). This study was undertaken to determine levels of IL-18 and IL-18 binding inhibition in the blood of patients with adult-onset Still's disease (ASD). METHODS: Serum concentrations of IL-18 in ASD patients were compared by enzyme-linked immunosorbent assay (ELISA) with those in patients with other systemic rheumatic diseases and healthy controls. The biologically active mature protein of IL-18 was detected by Western blot analysis with anti-IL-18 antibody and its induction of interferon-gamma (IFNgamma) secretion from IL-18-responding human myelomonocytic KG-1 cells. The inhibitory activity on IL-18 binding to its receptor was determined by 125I-IL-18 binding inhibition assay using the Chinese hamster ovary cell line transfected with a murine IL-18 receptor (CHO-K1/mIL-18R). RESULTS: Concentrations of serum IL-18 were extremely elevated in patients with active ASD compared with those in patients with rheumatoid arthritis, systemic lupus erythematosus, systemic sclerosis, polymyositis/dermatomyositis, Sjogren's syndrome, or healthy individuals. Levels of IL-18 were found to correlate with serum ferritin values and disease severity in ASD. Western blot analysis revealed that serum samples from patients with active ASD contained an 18-kd polypeptide of IL-18, corresponding in size to the mature form. Accordingly, the samples were able to induce IFNgamma secretion from KG-1 cells, which was largely abolished by neutralizing anti-IL-18 antibody. However, the level of IL-18 bioactivity was more than 10-fold weaker than the concentration of IL-18 protein measured by ELISA. Serum samples from patients with active ASD showed an inhibitory effect on the binding of 125I-IL-18 to CHO-K1/mIL-18R cells, and this activity was associated with elevation of IL-18. CONCLUSION: These data indicate that systemic overproduction of IL-18 may be closely related to the pathogenesis of ASD, despite the restriction on its inflammatory activity by IL-18 binding inhibitors such as IL-18BP. The disease activity appears to be determined on the basis of the relative levels of IL-18 and its specific inhibitors. 相似文献
40.
Komatsu H Fujimoto S Hara S Sato Y Yamada K Eto T 《Internal medicine (Tokyo, Japan)》2004,43(11):1023-1028
OBJECTIVE: The serum IgA/C3 ratio might be considered to serve as a diagnostic marker for patients with IgA nephropathy (IgAN), but its value as a marker of the severity of histological lesions or prognosis is unknown. METHODS: We studied the serum IgA/C3 ratio, using standardized reference material, in 86 patients with IgAN and in 32 with non-IgAN. The patients with IgAN were divided according to the severity of histological lesions (mild IgAN, n=29 and severe IgAN, n=57) based on Japanese clinical guidelines. RESULTS: The serum IgA level was significantly higher, while its C3 level was lower in patients with severe IgAN compared to those with non-IgAN. However, these levels were not different between patients with mild IgAN and non-IgAN. In contrast, the serum IgA/C3 ratio obviously differed among the three groups (2.47+/-0.96 vs. 3.63+/-1.44 vs. 4.72+/-1.86; p<0.01, ANOVA). Kaplan-Meier analysis of the patients with IgAN classified according to the mean serum IgA/C3 ratio revealed that the group with high serum IgA/C3 (4.5 and above) had a significantly poorer renal outcome (p<0.05, log-rank test), since the cumulative renal survival rate at 5 years was 84.4% vs. 100%. The ratio (%) of patients with severe IgAN in whom hematuria disappeared, was significantly higher in the low, than in the high serum IgA/C3 group (41.9% vs. 15.4%; p<0.05, t-test). CONCLUSION: The serum IgA/C3 ratio appears to reflect the histological severity of IgAN and could serve as a marker of the progression of IgAN. 相似文献