青藏高原鼠疫耶尔森菌基因型分布

目的研究青藏高原鼠疫耶尔森菌（鼠疫菌）基因组型分布特征.方法对分离到的青藏高原鼠疫菌297株,根据已经证实的22个差异区段设计引物,每株鼠疫菌的每个基因差异区段都采用PCR技术进行验证.结果在喜马拉雅旱獭鼠疫自然疫源地中,鼠疫菌基因组型有9种,分别为1、5、6、7、8、10、11、新基因组型和Ype-ancestor型,其中以5、8和10型为主,3种基因组型合计所占比例为80.6%（204/253）,而且不同地区鼠疫菌基因组型的分布也不一致.青藏高原青海田鼠鼠疫疫源地鼠疫菌基因组型全部为14型.结论青藏高原鼠疫菌基因组型分布具有明显的地理特征.根据基因组型的分布状况推测出了鼠疫菌在青藏高原的传播路径.     

硫酸镁对普鲁帕酮致心律失常的干预作用

目的探讨静脉滴注硫酸镁对普鲁帕酮致心律失常作用的干预效果。方法将92例心律失常患者随机分为对照组和干预组,每组46例。两组均口服普鲁帕酮,干预组同时静脉滴注硫酸镁,于治疗1周后常规心电图观察心律失常变化及致心律失常作用。结果心律失常消失率对照组为43.4%(20/46)、干预组为82.6%(39/46),致心律失常率对照组为19.6%、干预组为4.4%,两组比较有统计学意义(P<0.01)。结论普鲁帕酮抗心律失常同时有致心律失常作用,使用硫酸镁干预可降低致心律失常发生率。     

乌龙丹联合柳氮磺吡啶治疗强直性脊柱炎36例

目的：观察乌龙丹联合柳氮磺吡啶治疗强直性脊柱炎的疗效与安全性。方法：66例门诊患者随机分为治疗组36例和对照组30例,治疗组给予乌龙丹联合柳氮磺吡啶治疗,对照组给予柳氮磺吡啶治疗,两组均在必要时给予NSAIDs治疗并记录剂量及用药天数。观察治疗前后两组患者临床疗效及实验室指标改变情况。结果：治疗12周后,两组患者在晨僵时间、ESR、CRP、BASFI、BASDAI均比治疗前有显著改善（P＜0．05）,且治疗组优于对照组（P＜0．05）。治疗组有效率高于对照组（P＜0．05）,且不良反应较对照组少（ P＜0．05）。结论：乌龙丹联合柳氮磺吡啶治疗强直性脊柱炎有良好疗效。     

Correlations between mean LH levels and LH pulse characteristics: differences between normal and anovulatory women

OBJECTIVE: Since LH secretion occurs as a series of pulses, relationships between mean LH levels and LH pulse characteristics are to be expected. The aim of this study was to determine whether such relationships are similar in normal women and anovulatory patients. DESIGN: We studied the correlations between mean LH levels and the products amplitude x frequency and area x frequency of LH pulses in normal women and in patients with disorders of ovulation. Blood samples were taken from each subject every 10 minutes during 6 hours on the 8th day after the last menses. PATIENTS: The patients were divided into three groups: patients with polycystic ovary syndrome (n = 11), patients with idiopathic anovulation (n = 14) and patients with short luteal phase (n = 13). Their results were compared to those of 12 normal women. MEASUREMENTS: LH was evaluated with an immunoradiometric assay. LH data were analysed with a 3 standard deviation threshold criterion for significant peaks, and with cluster analysis algorithm using 1, 2.5 and 5% false positive error rates and 'optimal parameters' (which give less than 5% false positive and false negative error rates in LH male data). RESULTS: Highly significant correlations between amplitude x frequency, area x frequency and mean LH were found in normals and patients with short luteal phase; no significant correlation was found in patients with polycystic ovary syndrome, while significant correlations were found in patients with idiopathic anovulation only with some of our criteria for peak detection. CONCLUSION: The differences that we found between the groups suggest that when commonly used methods are employed to determine LH pulse characteristics, most of the significant LH pulses are taken into account in normals and patients with short luteal phase, but not in anovulatory patients, especially in patients with polycystic ovary syndrome. This method using two correlations appears to be a simple and useful way to show the differences in the mechanisms by which mean LH levels are achieved in normal subjects and patients.     

藏药石榴日轮丸的质量标准研究

目的：建立常用藏药石榴日轮丸的质量标准。方法：薄层色谱鉴别和薄层扫描含量测定。结果：鉴别出石榴日轮丸肉桂、红花和西藏棱子芹等药材，阴性样品对鉴别无干扰；测得石榴日轮丸中胡椒碱含量为0．4－0.5mg。结论：该研究提高了本品质量标准的专属性和规范性。     

藏药二十八味槟榔丸的鉴别及没食子酸的含量测定

目的　建立更为规范的藏药二十八味槟榔丸的质量标准。方法　采用TLC和HPLC。结果　TLC鉴别了二十八味槟榔丸中的槟榔、荜茇、诃子、姜黄、圆柏、小檗皮等 6味药材 ,并通过HPLC测定了 3批制剂中诃子的有效成份没食子酸的含量 ,分别为 0 .2 1 1 %、0 .2 0 9%、0 .2 1 6 % ,在 9.3～ 32 .5μg·ml- 1 的浓度范围内 ,线性关系良好 (r=0 9997)。平均回收率为 98.4% ,RSD =1 .2 7%。结论　鉴别的专属性明显提高 ,含量测定方法简便、准确。     

软骨脱细胞基质仿生支架的制备及其对骨髓基质干细胞成软骨分化的影响

目的探讨软骨脱细胞基质(ACM)仿生支架的制备及其对骨髓基质干细胞(BMSCs)成软骨分化的影响。方法将软骨组织粉碎后脱细胞处理,并以不同的比例与明胶(GT)溶液混合并交联,冷冻干燥制成多孔仿生支架。用扫描电镜(SEM)观察不同交联比例制成的ACM/GT支架,并对其孔径、孔隙率、生物力学、降解速率进行评估,从而选取最优组用于体外软骨构建。分离并培养山羊BMSCs,接种于单纯GT和ACM/GT支架上,SEM观察培养1、7、14 d后细胞在支架上的黏附、分布与基质分泌情况,并通过实时定量聚合酶链反应(qRT-PCR)评估成软骨分化能力。结果制备的ACM无细胞残留。随着ACM占比增加,孔径逐渐增大,降解速率逐渐增快,力学强度逐渐降低。其中G2A2组在孔径、孔隙率、生物力学和降解速率方面均适宜体外构建软骨组织。SEM显示细胞在单纯GT和G2A2支架上均匀分布,增殖显著,基质分泌明显。qRT-PCR显示G2A2显著促进了BMSCs的成软骨分化。结论ACM可以制备成适宜软骨再生的支架材料,并可促进BMSCs的成软骨分化。     

军队基层官兵睡眠疾病调查

  目的 调查研究基层官兵睡眠疾病的发生情况。 方法 采用问卷法、直接咨询法、针对性检查相结合的调查方法。选择30 种较常见的睡眠疾病作为调查内容,被调查者在调查者的指导下填写问卷。 结果 基层官兵普遍存在睡眠问题,按发病率高低依次为睡眠不足(41.36%)、失眠(38.42%)、鼾症(36.62%)、睡眠梦魇症(30.07%)、睡眠遗精症(29.30%)、睡眠梦语症(25.84%)、睡眠不安腿综合征(25.11%)、睡眠肌痉挛综合征(23.45%)、睡眠时相延迟综合征(23.11%)、半醒综合征(20.28%)、睡眠期多汗症(15.14%)、倒班综合征(14.96%)、短睡眠者(14.66%)、夜间下肢痛性痉挛症(13.86%)、磨牙症(13.63%)、失眠症(12.50%)、时差变化综合征(11.67%)、睡眠相关性痛性阴茎勃起(10.79%)、睡眠多尿症(9.96%)、睡眠时相提前综合征(8.79%)、睡眠窒息综合征(8.40%)、睡眠性头痛(8.27%)、睡眠呼吸暂停综合征(7.52%)、长睡眠者(6.79%)、睡眠惊叫(6.76%)、睡眠瘫痪症(6.09%)、睡眠性呼吸急促症(6.03%)、睡眠性胃食管反流症(6.02%)、睡眠异常吞咽综合征(4.50%)和睡眠性喉痉挛症(3.07%)。在30个项目中,有15项战士与干部比较差异有统计学意义(P＜0.05),发生率战士高于干部的有8项,低于干部的有7项。 结论 基层官兵睡眠疾病发生率较高,可影响官兵日常工作、训练和战斗力,应高度重视,并积极采取干预措施减少失眠和睡眠疾病的发生。     

Concomitant sickle cell disease and skeletal fluorosis

Skeletal fluorosis typically manifests as a diffuse increase in bone density, whereas avascular necrosis of the epiphyses and diaphyseal marrow are the main skeletal manifestations of sickle cell disease. The diagnostic and therapeutic challenges raised when both disorders are present are illustrated by two cases in Senegalese patients from an area characterized by high fluoride contents in the water and soil. Both had SS sickle cell disease. Skeletal fluorosis was diagnosed during evaluation for avascular necrosis in one patient and in the wake of septic arthritis in the other. Femoral head necrosis is difficult to identify in a patient with skeletal fluorosis. The bone lesions due to sickle cell disease and those due to fluorosis can mimic other bone diseases, most notably metastases. The combination of sickle cell disease and fluorosis results in significant medullary canal narrowing due to cortical thickening and to accumulation of necrotic bone. When performing hip replacement surgery, careful reaming of the medullary canal may reduce the risk of iatrogenic femoral fracture and inappropriate stem placement.