青年心肌致密化不全合并心力衰竭1例并文献复习

目的 系统回顾心肌致密化不全(NVM)的疾病特点及诊治要点,并总结该病的治疗经验.方法 回顾性分析2020年4月2—18日及9月2—7日该院心内科入院收治的1例NVM合并心律失常、心力衰竭及血栓栓塞等多种并发症的青年患者的诊治经过,并系统随访11个月,观察患者预后.结果 积极控制并纠正心律失常后患者心脏结构及功能呈现可逆性改善.结论 NVM是临床较为罕见的先天性心肌病.其临床表现多样而不典型,可出现包括心律失常、心力衰竭、血栓栓塞等多种不良事件,甚至可出现心源性猝死等恶性后果.临床医师需加强对该病的认识,为及时确诊并合理治疗提供可能.     

童年期心理虐待和忽视、述情障碍对大学生无聊倾向的影响

目的探讨童年期心理虐待和忽视、述情障碍对大学生无聊倾向的影响及其关系。方法采用大学生无聊倾向问卷(boredom proneness scale for college students,BPS)、儿童心理虐待和忽视量表(child psychological abuse and neglect scale,CPANS)、多伦多述情障碍量表(the Toronto alexithymia scale,TAS-20)对1557名大学生进行调查,采用SPSS 22.0进行描述性统计、相关分析和回归分析。结果童年心理虐待[(26.540±8.985)分]、忽视[(32.665±10.651)分]、述情障碍的识别情感维度[(19.359±4.835)分]、描述情感维度[(14.173±3.001)分],外向思维维度[(20.383±3.429)分]与无聊倾向总分[(101.109±19.735)分]、外部刺激维度[(66.022±21.079)分]存在正相关(r=0.160~0.559,均P<0.01),而与内部刺激维度[(35.132±8.207)分]存在负相关(r=-0.302~-0.104,均P<0.01)。分层回归分析显示,在控制性别和年龄的影响后,心理虐待与忽视是无聊倾向、内部刺激和外部刺激的影响因素,分别可解释总变异的14.3%,5.1%和18.2%(△F=130.561、41.968、172.711,均P<0.01)。述情障碍的识别情绪、描述情绪和外向思维是无聊倾向、内部刺激和外部刺激的影响因素,分别可解释总变异的17.5%,9.9%和25.5%(△F=134.274、60.696、234.383,P<0.01)。结论童年心理虐待和忽视、述情障碍对大学生无聊倾向存在显著影响,主要体现在对无聊倾向的外部刺激的影响上,其中述情障碍影响最大、解释力最高。     

Investigation of IL4 gene polymorphism in individuals with different levels of chronic periodontitis in a Brazilian population

BACKGROUND: Cytokines are key factors that mediate the inflammatory process during periodontal disease. Recent works have shown that the levels of cytokine expression are regulated by genetic polymorphisms, and that these variations can interfere with the progression of disease. The-590 (C-->T) polymorphism of the IL4 gene is associated with high levels of IgE in asthmatic families, and the frequency of the T allele was increased in asthmatic children. The concentration of IgE in gingival tissue was found to be elevated in patients with periodontitis. OBJECTIVE: In this study the relationship between the-590 (C-->T) polymorphism in the IL4 gene and different levels of chronic periodontal disease was investigated. MATERIAL AND METHODS: DNA was extracted from buccal epithelial cells of 113 unrelated adult individuals with different levels of periodontitis. The PCR-RFLP technique was used to investigate the polymorphism in the promoter of IL4 gene. RESULTS: No significant differences in the allele and genotype frequencies of the polymorphism were found between control and groups with periodontal disease. CONCLUSION: : We conclude that the-590 (C-->T) polymorphism in the IL4 gene is not associated with the susceptibility to chronic periodontal disease.     

Inverted U-Shaped Associations between Glycemic Indices and Serum Uric Acid Levels in the General Chinese Population: Findings from the China Cardiometabolic Disease and Cancer Cohort (4C) Study

Objective The relationship between serum uric acid(SUA)levels and glycemic indices,including plasma glucose(FPG),2-hour postload glucose(2 h-PG),and glycated hemoglobin(HbA1 c),remains inconclusive.We aimed to explore the associations between glycemic indices and SUA levels in the general Chinese population.Methods The current study was a cross-sectional analysis using the first follow-up survey data from The China Cardiometabolic Disease and Cancer Cohort Study.A total of 105,922 community-dwelling adults aged≥40 years underwent the oral glucose tolerance test and uric acid assessment.The nonlinear relationships between glycemic indices and SUA levels were explored using generalized additive models.Results A total of 30,941 men and 62,361 women were eligible for the current analysis.Generalized additive models verified the inverted U-shaped association between glycemic indices and SUA levels,but with different inflection points in men and women.The thresholds for FPG,2 h-PG,and HbA1 c for men and women were 6.5/8.0 mmol/L,11.0/14.0 mmol/L,and 6.1/6.5,respectively(SUA levels increased with increasing glycemic indices before the inflection points and then eventually decreased with further increases in the glycemic indices).Conclusion An inverted U-shaped association was observed between major glycemic indices and uric acid levels in both sexes,while the inflection points were reached earlier in men than in women.     

National Nutrition and Health Systematic Survey for Children 0-17 Years of Age in China

The main purpose of the National Nutrition and Health Systematic Survey for children 0-17 years of age in China (CNHSC) was to collect basic data on the nutrition, development, and health status for children in different regions across China using evidence-based, reliable, and cost-effective approaches. Children and their parents or guardians from seven regions (south, southwest, north, northwest, eastern, central, and northeast China) in China were recruited. A multi-stage stratified randomized sampling method was used. Two provinces were randomly sampled from each of the seven regions, from which one urban district and one rural country were also randomly sampled, resulting in a total of 28 survey counties/districts. Dietary surveys, health examinations, laboratory testing, and questionnaires were used to collect dietary intake, nutritional status, child development, and health status information. Nutrition, health, and lifestyle assessment of children and their parents was determined using the Knowledge Attitude Practice (KAP) survey. Greater than 100,000 children (38,000 children < 6 years of age and 66,000 children 6-17 years of age) completed the survey. The survey provided comprehensive data on child nutrition and health status for future studies and will serve as the basis for an integrated nutrition and health improvement strategies proposal for children in China.     

Proteomics Study on the Differentially Expressed Proteins in 16HBE Cells Exposed to Beryllium Sulfate

Beryllium (Be) is a non-radioactive element with carcinogenic properties, and presents serious occupational and environmental hazards from its resulting toxicants, which could significantly impact the health of the occupational population[1-3]. Beryllium and its compounds can cause diseases such as acute chemical pneumonia, lung cancer, and chronic beryllium disease, which predominantly occurs alongside either lung granuloma or pulmonary fibrosis[4,5]. Furthermore, the mechanism by which beryllium is toxic has not been fully elucidated; therefore, studies on its health hazards as well of its compounds are important. An in-depth study of the mechanism of beryllium toxicity is especially essential to prevent and control its associated health hazards. Tandem mass tag (TMT) technology is a reliable technology in quantitative proteomics, which can be implemented to perform relative quantification and identification analysis of proteins, peptides, nucleic acids, and other biological macromolecules.     

Comparative Study of the Genetic Diversity,Antimicrobial Resistance,and Pathogenicity of Aeromonas Isolates from Clinical Patients and Healthy Individuals

Objective This study was performed to compare the genetic diversity, virulence, and antimicrobial resistance of Aeromonas strains isolated from patients and healthy individuals. Methods A total of 38 clinical strains and 19 strains from healthy individuals were isolated from the samples collected in Ma'anshan City, Anhui Province. Their taxonomy was investigated using concatenated gyrB-cpn60 sequences, and their resistance to 12 antibiotics was evaluated. The pathogenicity of these strains was examined through beta-hemolysis, protease activity, and virulence gene assays. Results The 57 Aeromonas strains were divided into 55 sequence types. Of these types, 21 were novel, suggesting that their genetic diversity was high. These Aeromonas isolates could be divided into 7 species, and the positive rates of beta-hemolysis and protease activity were 49.1％ and 73.7％, respectively. The detection rate of clinical patients in terms of beta-hemolysis and protease activity was higher than that of healthy individuals. Among the four most common Aeromonas strains, A. dhakensis had the highest detection rate of virulence genes. The multidrug resistance rate of the clinical isolates was much higher than that of the strains isolated from healthy individuals. Conclusions The taxonomy, virulence properties, and antibiotic resistance of Aeromonas isolates from patients differ from those of the isolates from healthy individuals.     

补肺化积汤治疗晚期非小细胞肺癌

目的 观察中药支持和补肺化积汤治疗晚期非小细胞肺癌的效果.方法 将60例非小细胞肺癌患者随机分为治疗组与对照组,各30例.对照组采用艾迪注射液做支持疗法,治疗组在此基础上配合补肺化积汤治疗.治疗3个疗程后观察2组临床疗效.结果 治疗组瘤体的缓解程度、Karnofsky评分等均优于对照组.结论 补肺化积汤对正虚毒结型非小细胞肺癌具有良好的治疗效果.     

Mash1 efficiently reprograms rat astrocytes into neurons简

To date, it remains poorly understood whether astrocytes can be easily reprogrammed into neurons. Mashl and Brn2 have been previously shown to cooperate to reprogram fibroblasts into neurons. In this study, we examined astrocytes from 2-month-old Sprague-Dawley rats, and found that Brn2 was expressed, but Mashl was not detectable. Thus, we hypothesized that Mashl alone could be used to reprogram astrocytes into neurons. We transfected a recombinant MSCV-MASH1 plasmid into astrocytes for 72 hours, and saw that all cells expressed Mashl. One week later, we observed the changes in morphology of astrocytes, which showed typical neuro- nal characteristics. Moreover, β-tubulin expression levels were significantly higher in astrocytes expressing Mashl than in control cells. These results indicate that Mashl alone can reprogram astrocytes into neurons.