Stability and aging resistance of a zirconia oral implant using a carbon fiber-reinforced screw for implant-abutment connection

Objective

To investigate the long-term stability of a metal-free zirconia two-piece implant assembled with a carbon fiber-reinforced (CRF) screw by means of transformation propagation, potential changes in surface roughness, the gap size of the implant-abutment connection, and fracture load values.

Optimal esthetics in single-tooth replacement with the Re-Implant system: a case report.

PURPOSE: This report demonstrates the use of root-analogue titanium implants for single-tooth replacement. MATERIALS AND METHODS: A maxillary lateral incisor was removed and a custom-made one-stage, root-analogue titanium implant (Re-Implant) with an apical extension and a healing cap was fabricated and placed immediately after tooth extraction. Six months later the healing cap was removed, an impression was taken, and a porcelain-fused-to-metal crown was fabricated and cemented. RESULTS: No complications occurred during the healing period. A good esthetic result was achieved with the final ceramometal crown. Bony resorption and buccal soft tissue recession led to a slight discoloration of the marginal periimplant mucosa. CONCLUSION: Further research into the modalities of the immediate placement of root-analogue implants is needed to obtain predictable esthetic results concerning the soft tissue environment.     

Effect of different crown contours on periodontal health in dogs. Clinical results

OBJECTIVES: The aim of this study is to evaluate clinically the effect of different crown contours on the periodontium in beagle dogs. METHODS: One month after commencing oral hygiene procedures the second and third premolars in three quadrants of four adult beagle dogs were prepared for receiving crowns. The unprepared second and third premolars in one quadrant served as controls. Crowns with three different emergence profiles (normal (=tooth) contour, 30 degrees and 50 degrees over-contour) were cemented where one quadrant received the same treatment. Clinical parameters were recorded on all four sites (mesial, buccal, distal and lingual) of test and control teeth at baseline, after 3 and 5 months. During the entire study period, professional oral hygiene was performed seven times a week. RESULTS: The Plaque Index increased for the 30 degrees and 50 degrees over-contour groups, although no statistically significant differences could be observed. For the normal contour and control group minor changes occurred. Similar results could be depicted for the Gingival Index. The changes regarding the variables Plaque and Gingival Index were correlated to the Gingival Crevicular Fluid Flow. Here, significant differences for the Gingival Crevicular Flow were observed between the treatment groups and over the treatment period for the 30 and 50 degrees over-contour groups. Only slight differences for this parameter were observed in and between the control and normal contour groups. In the control group, the Pocket Probing depth remained stable during the entire study but increased in the crowned treatment groups over time. At 3 and 5 months the probing depth was significant greater in the 30 degrees and 50 degrees over-contour groups compared to the control group. There was a higher loss of clinical attachment levels for the test groups than for the control group. There were statistically significant differences at each timepoint between groups and also within groups except for the control group. CONCLUSIONS: Within the limits of this study it can be concluded that over-contoured subgingival crowns seem to affect clinical periodontal health only slightly in dogs over 5 months when an intensive oral hygiene regimen is executed.     

On-line analysis of CAL72 cells on two different titanium surfaces in a perfusion micro-bioreactor.

OBJECTIVES: The aim of the present experiment was to test a prototype microsensoric measuring system (micro-bioreactor) for the investigation of the biocompatibility of different titanium surfaces in a cell culture model. METHODS: Osteosarcoma cells of the cell line CAL72 were seeded onto titanium plates (10mm x 10mm x 1mm) and inserted into the culture chamber of the micro-bioreactor. Titanium plates with two different surface topographies (machined and titanium plasma-sprayed [TPS]) were used for this pilot investigation. Plastic plates served as controls. The online-sensoric device of the micro-bioreactor allowed the continuous monitoring of the metabolism of the cells and the control of the culture conditions. Over a period of 17 h changes in O(2)-consumption in the medium were measured by micro-electrodes and registered by the software of the system. The metabolic activity of the cells was calculated from the difference between the bypass and the chamber values. The cell proliferation and vitality were analyzed before and after the perfusion time in the micro-bioreactor. The cell morphology was studied using scanning electron microscopy. RESULTS: The cells on the machined surfaces showed the highest oxygen consumption after 15 h, after that it decreased. The cells on the TPS plates showed a lower oxygen consumption, which remained stable after 17 h. The highest oxygen consumption was seen with the cells on the control plastic plates. Concerning cell proliferation analysis, it could be shown that more vital CAL72 cells seeded onto TPS and plastic could be detected after the passage through the micro-bioreactor. Hence, the number of vital cells on the machined surface was reduced after the passage. SIGNIFICANCE: Within the limits of this experiment, the presented micro-bioreactor system could offer a valuable method to examine the dynamic interactions of cells and materials under defined in vitro experimental conditions. While the presented system is already successfully used in the ecological/ecotoxicological field, its routine use for investigating dental materials on a cellular level has to be evaluated.     

Stability of prototype two-piece zirconia and titanium implants after artificial aging: an in vitro pilot study

Background: Zirconia oral implants are a new topic in implant dentistry. So far, no data are available on the biomechanical behavior of two‐piece zirconia implants. Therefore, the purpose of this pilot investigation was to test in vitro the fracture strength of two‐piece cylindrical zirconia implants after aging in a chewing simulator. Materials and Methods: This laboratory in vitro investigation comprised three different treatment groups. Each group consisted of 16 specimens. In group 1, two‐piece zirconia implants were restored with zirconia crowns (zirconia copings veneered with Triceram®; Esprident, Ispringen, Germany), and in group 2 zirconia implants received Empress® 2 single crowns (Ivoclar Vivadent AG, Schaan, Liechtenstein). The implants, including the abutments, in the two zirconia groups were identical. In group 3, similar titanium implants were reconstructed with porcelain‐fused‐to‐metal crowns. Eight samples of each group were submitted to artificial aging with a long‐term load test in the artificial mouth (chewing simulator). Subsequently, all not artificially aged samples and all artificially aged samples that survived the long‐term loading of each group were submitted to a fracture strength test in a universal testing machine. For the pairwise comparisons in the different test groups with or without artificial loading and between the different groups at a given artificial loading condition, the Wilcoxon rank‐sum test for independent samples was used. The significance level was set at 5%. Results: One sample of group 1 (veneer fracture), none of group 2, and six samples of group 3 (implant abutment screw fractures) failed while exposed to the artificial mouth. The values for the fracture strength after artificial loading with 1.2 million cycles for group 1 were between 45 and 377 N (mean: 275.7 N), in group 2 between 240 and 314 N (mean: 280.7 N), and in the titanium group between 45 and 582 N (mean: 165.7 N). The fracture strength results without artificial load for group 1 amounted to between 270 and 393 N (mean: 325.1 N), for group 2 between 235 and 321 N (mean: 281.8 N), and between 474 and 765 N (mean: 595.2 N) for the titanium group. The failure mode during the fracture testing in the zirconia implant groups was a fracture of the implant head and a bending/fracture of the abutment screw in the titanium group. Conclusions: Within the limits of this pilot investigation, the biomechanical stability of all tested prototype implant groups seems to be – compared with the possibly exerted occlusal forces – borderline for clinical use. A high number of failures occurred already during the artificial loading in the titanium group at the abutment screw level. The zirconia implant groups showed irreparable implant head fractures at relatively low fracture loads. Therefore, the clinical use of the presented prototype implants has to be questioned.