Impact of irrigation systems on stone migration

PURPOSE: To evaluate forces exerted on a stone with different ureteroscopic irrigation systems that could impact stone migration during ureteroscopy. MATERIALS AND METHODS: A 3-mm steel simulated stone was welded to a 3F stone basket. The basket wire was then backloaded into the working channel of a 4.5F Wolf semirigid ureteroscope. The basket shaft was attached to a 50 g load cell. The ureteroscope was placed in a ureteral model (14F silicon tubing). Simulated blood was dripped adjacent to the stone at 12 drops/min. Endo-irrigation devices were attached to the ureteroscope, and irrigation was applied at a rate sufficient to maintain visualization of the stone. Force on the stone was measured with the following endo-irrigation systems: Gravity (183 cm H2O); pressurized irrigation (150 mm Hg, 300 mm Hg); Kosin Technology Universal Piggyback Irrigation System (UPIS) at gravity (183 cm H2O), 150 mm Hg and 300 mm Hg; EMS Medical Peditrol Foot Pump; Cook Ureteroscopy Irrigation System; ACMI Irri-Flo Irrigation Delivery System; and Boston Scientific Single-Action-Pump System (SAPS). RESULTS: SAP required the least number of pumps (0.35/sec) to maintain a clear endoscopic field while the Peditrol device required the most (1.88/sec). Pulse duration was longest for the ACMI (1.6 sec) and shortest for pressurized gravity and UPIS (<0.3 sec). The average total maximum impulse during a pump was significantly greater with the Cook (0.017 Ns) and ACMI (0.027 Ns) systems. On average, gravity-based systems applied the least amount of force, < or =0.0006 Ns, maximum impulse at any given time. CONCLUSIONS: Gravity-based systems exert less force than hand-held and foot-pump devices. Of the hand-held devices, the SAP exerted the least average maximum impulse on the stone.     

Anorectal stimulation causes increased colonic motor activity in subjects with spinal cord injury

BACKGROUND: Difficulty with evacuation (DWE) is a major problem after spinal cord injury (SCI). Stimulation of the anal canal and lower rectum, accomplished using a gloved finger (so-called digital rectal stimulation or DRS) is often used as an adjunct to laxatives and enemas to facilitate bowel evacuation. However, the basis for the efficacy of DRS is not known. This study assessed the effect of DRS on colonic motility. METHODS: Six subjects with SCI were studied several hours after a bowel care session. Colonic motility was assessed using a manometric catheter (affixed endoscopically to the splenic flexure) at baseline, during DRS, and after DRS. In addition, evacuation of barium oatmeal paste (with the consistency of stool and introduced into the rectum and descending colon) was assessed simultaneously using fluoroscopic techniques. RESULTS: The mean number (+/- SEM) of peristaltic waves per minute increased from 0 at baseline to 1.9 (+/- 0.5/min) during DRS and 1.5 (+/- 0.3/min) during the period immediately after cessation of DRS (P < 0.05). The mean amplitude (+/- SEM) of the peristaltic contractions was 43.4 (+/- 2.2) mmHg. The frequency of contractions, as well as amplitude of contractions, during or immediately after DRS was not significantly different. These manometric changes in response to DRS were accompanied by expulsion of barium oatmeal paste in every subject by the fifth DRS. CONCLUSIONS: DRS causes left-sided colonic activity in subjects with SCI. At least in part, an anorectal colonic reflex that results in enhanced contractions of the descending colon and rectum may contribute to bowel evacuation in individuals with SCI.     

MSH2 118T>C and MSH6 159C>T promoter polymorphisms and the risk of colorectal cancer

The most important indicator of colorectal cancer (CRC) risk is the presence of family history of the disease. Inherited genetic changes, such as single nucleotide polymorphisms, in key candidate genes may contribute to CRC risk. We investigated whether promoter polymorphisms in DNA mismatch repair (MMR) genes MSH2 and MSH6 are associated with the risk of CRC. We genotyped 929 CRC patients and 1098 control subjects from Ontario, and 467 patients and 344 controls from Newfoundland and Labrador, for two promoter polymorphisms in the MMR genes MSH2 and MSH6 using the fluorogenic 5' nuclease assay. We used unconditional logistic regression to evaluate the association between each polymorphism and CRC after adjusting for age and sex. The associations between polymorphisms and tumor clinicopathological features were evaluated with a Pearson's chi-squared test or Fisher's exact test. All statistical tests were two sided. We observed strong associations between the MSH2 -118T>C polymorphism and family history of CRC based on the Amsterdam criteria I (P = 0.005) and Amsterdam criteria I and II (P = 0.036) among cases from Ontario. This association was especially evident among female CRC patients in Ontario (for Amsterdam criteria I, and I and II combined, P = 0.003 and P = 0.0001, respectively). The MSH2 -118T>C polymorphism was associated with strong family history of CRC in Ontario patients.