一种FⅨ基因多态性在广西人群中的检出

目的研究广西人群ＦⅨ（凝血因子Ⅸ）基因多态性。方法利用聚合酶链反应（ＰＣＲ）和限制性内切酶Ｔｒｕ９Ｉ（同功酶即ＭｓｅＩ）酶解技术，对１２０名志愿者进行研究。结果首次在广西人群中发现ＦⅨ基因５′侧翼６９８位核苷酸存在Ｔｒｕ９Ｉ多态性，该多态性由８３ｂｐ／５８ｂｐ＋２５ｂｐ组成，二者的基因频率在受检的１８０个ＦⅨ基因中，分别为０．６２２和０．３７８，女性杂合子频率为０．４７３。结论该发现有助于血友病乙携带者的检测及产前基因诊断。     

脾脏肿块的超声诊断

目的 :探讨脾脏肿块的超声表现及鉴别要点。材料和方法 :回顾性分析 76例脾脏肿块患者的超声检查资料 ,分析脾脏肿块的大小、数目、边界、内部回声等多种超声表现。结果 :76例脾脏肿块中有 3例错构瘤 ,10例血管瘤 ,16例淋巴瘤 ,2例淋巴管瘤 ,4例血管肉瘤 ,11例脾转移瘤 ,16例脾囊肿 ,4例脾梗死 ,3例脾血肿 ,5例脾脓肿 ,2例付脾 ,其超声表现各具特征。结论 :超声检查可早期发现并区别脾脏肿块的囊、实性 ,借助彩色血流显像可进一步鉴别良、恶性病灶。     

股骨头缺血坏死的低场MRI评价

目的: 分析股骨头缺血坏死(ANFH)低场MRI征象,评价ANFH的MRI分期及其关节积液的分级.材料和方法:在分析50例正常髋MRI基础上回顾性对照分析51例(90个股骨头)ANFH的低场MRI和X线平片资料.结果:90个ANFH中,20个(22 %) X线平片为阴性;64个(71%)MRI示"脂肪强度征"即坏死区内或近侧可见类似皮下脂肪样信号.按照 Hungerford分期:0期9个,Ⅰ期13个,Ⅱ期31个,Ⅲ期26个,Ⅳ期11个.ANFH引起的关节积液多为2～3级(60个髋,占67%);Ⅲ、Ⅳ期ANFH合并关节积液量比0～Ⅱ期明显多(P＜0.005).结论:低场MRI能够准确显示ANFH的范围、程度、信号特征及其关节积液的情况,并能够对早期ANFH作出可靠诊断;"脂肪强度征"为低场MRI的特征性表现,对诊断ANFH有非常重要的价值.     

ASSAY OF S100 LANGERHANS' CELLS IN CERVICAL CARCINOMA

Langerhans cells (LCs) in the normal cervix (control, 19 cases), cervical carcinoma in situ( CIS, 19 cases) and invasive cervical carcinoma (30 cases), interdigitatlng cells (IDCs) and follicular dendritic cells (FDCs) in obturator lymph node draining Invasive cervical carcinoma were quantitatively assessed by ABC immunohistochemical staining using anti-S100 protein antibody. The results indicated that S100 LCs in sltu, S100 IDCs and S100 FDCs In obturator lymph node showed dendritic features with a specific distribution. Number of LCs in situ in Invasive carcinoma increased significantly when compared with CIS and control groups. There was no change in LCs number between grade Ⅱ and Ⅲ of squemous cell carcinoma. Number of IDCs was significantly less in stage Ⅱ than in stage Ⅰ . FDCs number In different Invasive depthes and clinical stages showed no obvious change. The results suggest that progression of cervical carcinoma is closely related to decrease of LCs hi situ and IDCs in regional lymph n     

Inhibitory effect of glycyrrhiza uralensis fish and chelidonium majus L on gastrocarcinogenesis induced by N-methyl-N′-nitron-N-nitrosoguanidine

In order to investigate the antagonistic effect of Glycyrrhiza Uralensis Fish (GUF) and Chelidonium maJus L (CML) on gastrccarcinogenesis induced by MNNG in Wastar rats, we treated the rats with MNNG alone (group 1) and with MNNG plus GUF and CML (group 2 and 3) respectively. The incidence of infiltrating adenocarcinoma of the glandular stomach and duodenum in group 2 was significantly lower than that in group 1 (26.7% vs. 67.8%). The differentiation and aggressivenees of carcinomas occured in group 2 were much better and mild than those in group 1. Present study also demonstrated that the inhibitory effect of CML on proliferation of human stomach carcinoma cell line MGC-803 was very remarkable; in addition, GUF and CML were able to antagonise the mutagenic activation of MNNG. These results suggest that GUF and CML may be empoyed in prevention of gastric carcinoma.     

应用气相色谱法鉴别海南省大劣按蚊

大劣按蚊是海南省疟疾的主要传播媒介。本文应用气相色谱法,分析海南省不同地区、不同季节大劣按蚊的皮质烃类。经统计学分析,它们的成分无显著性差异。应用聚类分析法,它们的距离系数很接近。提示海南省的大劣按蚊很可能是同一种。     

Nd∶YAG激光后发性白内障切开技术的探讨

为探讨Ｎｄ∶ＹＡＧ激光治疗后发性白内障的技术问题，回顾了３４例３５只眼Ｎｄ∶ＹＡＧ激光治疗后发性白内障的类型、击发能量与脉冲、切开方法和术后效果。从Ｎｄ∶ＹＡＧ激光的聚焦、能量和切开方法的选择、病人视轴区的确定与术后并发症的观察处理方面，探讨了Ｎｄ∶ＹＡＧ激光后发性白内障的切开技术问题。结果表明，Ｎｄ∶ＹＡＧ激光治疗后发性白内障安全有效。为了避免损伤焦点周围的光学界面组织，必须有足够的功率密度和准确的聚焦，从最小能量开始，逐渐增加，直到出现切割效果。由于后发性白内障厚薄、机化程度以及有无人工晶体、虹膜粘连、后囊破裂、玻璃体脱出和偏位激光孔等不同情况，手术时选用适当的切开方法也十分重要。     

脑肿瘤误诊原因分析

本文23例脑肿瘤误诊为其他神经内科疾病12种,其中误诊为脑血管病15例,占65%.作者结合文献对其误诊原因作丁分析:1.发病年龄、形式、临床过程及局灶体征均貌似脑血管病。2.忽略某些肿瘤慢性进展的特点。3.颅内高压不明显.4.局灶性癫痫反复发作对肿瘤诊断上的重要意义。5.临床表现形式特殊:如偏头痛,痴呆等.     

下丘脑弓状核注射神经降压素的镇痛效应及机制探讨

采用微量注射法,对37只大鼠进行了实验观察.结果如下:①下丘脑弓状核(ARC)注射神经降压素(NT)可使甩尾反应潜伏期(TFL)或痛阈显著升高.对照组注射生理盐水,TFL无明显变化.②ARC先注射纳洛酮,再注射NT,TFL变化值与单纯注射NT组相比明显下降.③ARC注射β-内啡肽(β-End)抗血清,再注射NT,TFL显著下降.④ARC注射生理盐水不影响注射NT的镇痛效应.上述结果表明,大鼠ARC注射NT可产生明显的镇痛效应,该效应可被ARC预先注射纳洛酮或β-内啡肽抗血清翻转,提示NT在ARC的镇痛作用,部分是由β-End介导的.