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21.
Guerrero M Harjai K Stone GW Brodie B Cox D Boura J Grines L O'Neill W Grines C 《The American journal of cardiology》2005,96(5):649-654
Patients with acute myocardial infarction (AMI) often have multiple co-morbidities that influence outcome. We sought to evaluate the impact of peripheral vascular disease (PVD) on the outcome of patients with AMI treated with primary angioplasty. We evaluated 3,716 patients with AMI who underwent emergency catheterization with planned primary angioplasty in the Primary Angioplasty in Myocardial Infarction trials. Patients with a history of PVD (claudication, stroke, or transient ischemic attack) were compared with patients without PVD. Of the 3,716 patients, 394 (10.6%) had PVD and were older, more often women, and more frequently had a history of diabetes mellitus, hypertension, smoking, congestive heart failure, angina, myocardial infarction, and coronary revascularization. They presented more often with a heart rate >100 beats/min, Killip class >1, lower ejection fraction, and multivessel disease. No difference was found in stent use, final percentage of stenosis, or Thrombolysis In Myocardial Infarction 3 flow. Patients with PVD had a twofold increased in-hospital mortality (5.3% vs 2.6%, p = 0.0021). The difference remained significant at 1 month, 6 months, and 1 year (12.6% vs 6%, p < 0.0001). In multivariate logistic regression analysis, a history of PVD was an independent predictor of in-hospital mortality and death at 1 year (odds ratio 1.64, 95% confidence interval 1.04 to 2.57, p = 0.032). In conclusion, patients with AMI with PVD have increased co-morbidities and higher mortality despite treatment with primary angioplasty. The presence of PVD is an independent predictor of in-hospital mortality and death at 1 year. 相似文献
22.
Sheiban I Albiero R Marsico F Dharmadhikari A Tzifos V Pagnotta P Montorfano M Leonardo F Saba P Di Mario C Colombo A 《The American journal of cardiology》2000,85(9):1141-4, A9
The technique of "T" stenting, which incorporates wiring both the branches of a bifurcation lesion, dilating a balloon in the main branch during stent deployment in the side branch followed by deployment of stent in the main branch, provides excellent immediate results in terms of technical and procedural success. However, further innovations are mandated to optimize the long-term outcome, which with a restenosis rate of 63%, can be described as not encouraging. 相似文献
23.
Tiago Castro e Couto Mayra Yara Martins Brancaglion António Alvim-Soares Lafaiete Moreira Frederico Duarte Garcia Rodrigo Nicolato Regina Amélia Lopes P Aguiar Henrique Vitor Leite Humberto Corrêa 《World Journal of Psychiatry》2015,5(1):103-111
Postpartum depression is one of the most prevalent psychopathologies. Its prevalence is estimated to be between 10% and 15%. Despite its multifactorial etiology, it is known that genetics play an important role in the genesis of this disorder. This paper reviews epidemiological evidence supporting the role of genetics in postpartum depression (PPD). The main objectives of this review are to determine which genes and polymorphisms are associated with PPD and discuss how this association may occur. In addition, this paper explores whether these genes are somehow related to or even the same as those linked to Major Depression (MD). To identify gaps in the current knowledge that require investigation, a systematic review was conducted in the electronic databases PubMed, LILACS and SciELO using the index terms “postpartum depression” and “genetics”. Literature searches for articles in peer-reviewed journals were made until April 2014. PPD was indexed 56 times with genetics. The inclusion criteria were articles in Portuguese, Spanish or English that were available by institutional means or sent by authors upon request; this search resulted in 20 papers. Genes and polymorphisms traditionally related to MD, which are those involved in the serotonin, catecholamine, brain-derived neurotrophic factor and tryptophan metabolism, have been the most studied, and some have been related to PPD. The results are conflicting and some depend on epigenetics, which makes the data incipient. Further studies are required to determine the genes that are involved in PPD and establish the nature of the relationship between these genes and PPD. 相似文献
24.
Caroline COTES Mayra CARDOSO Renata Marques de MELO Luiz Felipe VALANDRO Marco Antonio BOTTINO 《Journal of applied oral science : revista FOB》2015,23(1):71-78
Objective
The purpose of this study was to assess the influence of conditioning methods and thermocycling on the bond strength between composite core and resin cement.Material and Methods
Eighty blocks (8×8×4 mm) were prepared with core build-up composite. The cementation surface was roughened with 120-grit carbide paper and the blocks were thermocycled (5,000 cycles, between 5°C and 55°C, with a 30 s dwell time in each bath). A layer of temporary luting agent was applied. After 24 h, the layer was removed, and the blocks were divided into five groups, according to surface treatment: (NT) No treatment (control); (SP) Grinding with 120-grit carbide paper; (AC) Etching with 37% phosphoric acid; (SC) Sandblasting with 30 mm SiO2 particles, silane application; (AO) Sandblasting with 50 mm Al2O3 particles, silane application. Two composite blocks were cemented to each other (n=8) and sectioned into sticks. Half of the specimens from each block were immediately tested for microtensile bond strength (µTBS), while the other half was subjected to storage for 6 months, thermocycling (12,000 cycles, between 5°C and 55°C, with a dwell time of 30 s in each bath) and µTBS test in a mechanical testing machine. Bond strength data were analyzed by repeated measures two-way ANOVA and Tukey test (α=0.05).Results
The µTBS was significantly affected by surface treatment (p=0.007) and thermocycling (p=0.000). Before aging, the SP group presented higher bond strength when compared to NT and AC groups, whereas all the other groups were statistically similar. After aging, all the groups were statistically similar. SP submitted to thermocycling showed lower bond strength than SP without thermocycling.Conclusion
Core composites should be roughened with a diamond bur before the luting process. Thermocycling tends to reduce the bond strength between composite and resin cement. 相似文献25.
Mayra S. Coutinho Eloah Latocheski Jannyely M. Neri Ana C. O. Neves Josiel B. Domingos Lívia N. Cavalcanti Luiz H. S. Gasparotto Edgar P. Moraes Fabrício G. Menezes 《RSC advances》2019,9(51):30007
The development of nanoprobes for selective detection of metal ions in solution has attracted great attention due to their impact on living organisms. As a contribution to this field, this paper reports the synthesis of silver nanoparticles modified with rutin in the presence of ascorbic acid and their successful use as a chromogenic probe for the selective detection of Fe3+ in aqueous solution. Limits of detection and quantification were found to be 17 nmol L−1 and 56 nmol L−1, respectively. The sensing ability is proposed to proceed via an iron-induced nanoparticle growth/aggregation mechanism. A practical approach using image analysis for quantification of Fe3+ is also described.The use of rutin-modified silver nanoparticles for selective detection and sensitive quantification of Fe3+ in aqueous solution is described. Metal ions are key species in nature due to their essential functions in living organisms.1,2 On the other hand, heavy metals as well as essential metals at abnormally high levels are toxic.2 Iron, for instance, in addition to its popular use in industry and construction, is essential to the human body and active in biological processes. Although the trivalent form of iron is particularly important for oxygen transport in blood and the mitochondrial respiratory chain, high levels of this cation are associated with important pathologies.3,4 The detection of metal ions in aqueous solution is traditionally performed by methods including atomic absorption spectrometry,5 electrochemical measurements,6,7 and inductively coupled plasma techniques,8 among others. However, these techniques have important drawbacks, notably the need for sophisticated instrumentation, in addition to being time-consuming and requiring laborious procedures. To overcome these issues, the development of chromogenic and fluorogenic chemosensors for the selective detection of metal-targets has attracted great attention, especially due to the possibility of fast, sensitive and non-expensive analysis.9,10 In the last decade, nanoscaled materials have been reported as selective probes for metal ions, including Fe3+.11–24Silver nanoparticles (AgNPs) are of particular interest because of the affordable price of starting materials, ease of controlling size and morphology, possibility to functionalize their surface with organic molecules, and optical properties that enable detection of a variety of analytes via simple UV-vis spectroscopy and digital image analysis. Furthermore, applications of AgNPs are also biotechnologically relevant due to the possibility of green synthetic protocols, including the use of plant extracts,25 natural sources,26 glycerol,27 among others.Flavonoids are secondary metabolites naturally found in fruits and other vegetables with relevant roles due to their nutritional, pharmaceutical and medicinal properties.28 Because of their adequate structural features, flavonoids are candidates to be employed in the synthesis of AgNPs.26 Rutin (RU), a sugar-based flavonoid, may be employed as reducing agent in the synthesis of AgNPs along with a stabilizer such as polyvinylpyrrolidone (PVP)26 or used as crude plant extract component.29,30This paper reports the use of RU-modified AgNPs (RU-AgNPs) as a chromogenic probe for Fe3+ in aqueous medium in the presence of ascorbic acid (AA). Sensing ability of RU-AgNPs for the selective detection of Fe3+ toward other metal cations was investigated with UV-vis spectroscopy analysis. These data and transmission electronic microscopy (TEM) results allowed a mechanistic proposal involved in the selective detection of Fe3+ by RU-AgNPs. Furthermore, a practical approach based on correlation of images of solutions obtained with a conventional smartphone and chemometrics was employed for a simpler quantification of Fe3+ in aqueous medium.Initially, the order of reagent combination was investigated in the synthesis of RU-AgNPs. Concentration of RU ranged from 0.10 to 0.50 mmol L−1, while concentrations of other components were fixed at 0.20 mmol L−1 AgNO3, 0.10 mmol L−1 AA and 0.10 M NaOH. Water was used as solvent in all cases. Narrower surface plasmon resonance (SPR) bands were obtained from adding a solution of AA and NaOH to a solution containing RU and AgNO3 (Fig. 1a) against the addition of RU, AA, and NaOH to AgNO3 solution (Fig. S1a – ESI†), or the addition of RU and NaOH to a solution of AA and AgNO3 (Fig. S1b†). RU-AgNPs obtained from the condition presented in Fig. 1a are small (4.1 nm average diameter) and considerably polydisperse (standard deviation of 4.7 nm), however, presenting only one population (Fig. 1b and S2†). A study of the influence of RU concentration (0.10 to 0.50 mmol L−1) on the stability of RU-AgNPs over time indicated that 0.10 mmol L−1 RU generates more stable RU-AgNPs (Fig. S3†). Next, a study on the influence of pH indicated that RU-AgNPs are only stable under strong alkaline conditions (pH 12.5 or higher) (Fig. S4†).Open in a separate windowFig. 1(a) UV-vis analysis of RU-AgNPs under strong alkaline condition (pH > 12.5) based on the order of adding reagents (RU, 0.10 to 0.50 mmol L−1; AgNO3, 0.20 mmol L−1; AA, 0.10 mmol L−1; NaOH, 0.10 mmol L−1); (b) TEM image of RU-AgNPs under the selected condition.The ability of RU-AgNPs to sense metal cations was investigated by both naked-eye and UV-vis spectroscopy analysis (Fig. 2). The separate addition of 10 μmol L−1 of several metal cations (Fe3+, Co2+, Zn2+, Sr2+, Cu2+. Al3+, Ba2+, Cd2+, Pb2+, Ni2+, Mg2+, Hg2+, Cu+ and Cr3+) to solutions of RU-AgNPs (prepared according to Fig. 1a) indicated that only Fe3+ induces a significant colorimetric change in the final aspect of solution after 50 minutes (Fig. 2a).Open in a separate windowFig. 2Naked-eye (a) and UV-vis (b) analysis of RU-AgNPs in absence (control, C) and presence of 10 μmol L−1 of selected metal cations (Fe3+, Co2+, Zn2+, Sr2+, Cu2+. Al3+, Ba2+, Cd2+, Pb2+, Ni2+, Mg2+, Hg2+, Cu+ and Cr3+) after 50 min; (c) calibration curve obtained by the addition of different amounts (0 to 10 μmol L−1) of Fe3+ to solutions of RU-AgNPs; (d) TEM image of RU-AgNPs after addition of Fe3+ (10 μmol L−1). In all experiments, RU-AgNPs were prepared in the presence of ascorbic acid.The results presented in Fig. 2a are consistent with the UV-vis spectroscopy analysis (Fig. 2b). Co2+ ions also induce some change in the system, however at a considerably smaller extension than Fe3+. In this study, AA played a crucial role in the selective detection of Fe3+ by the referred nanoprobe. In the absence of AA, Co2+ (mainly) as well as other cations induce stronger colorimetric and spectral changes (Fig. S5a and b,† respectively) in the analysis of solutions of RU-AgNPs when compared to the system containing AA. This selectivity may arise from two possible reasons: (i) preservation of RU by avoiding its oxidation in the reduction of Ag+ ions; (ii) coordination of ascorbate anion to cations other than Fe3+.Interaction of RU-AgNPs and Fe3+ (10 μmol L−1) stabilizes after approximately 40 minutes (Fig. S6†). Next, a calibration curve was built from the direct relationship between the absorbance at 396 nm and the concentration of Fe3+ (Fig. 2c), presenting a good correlation (R2 = 0.9929). The limits of detection and quantification were found to be 17 nmol L−1 and 56 nmol L−1, respectively, which is very satisfactory.13,14 The influence of other cations in the detection of Fe3+ was investigated by UV-vis spectroscopy. Fig. S7† clearly demonstrates that there are only small changes when a second cation (30 μmol L−1) is added together with Fe3+ to the RU-AgNPs solution.Mechanistically, the detection of Fe3+ by RU-AgNPs in aqueous medium proceeds via a growth/aggregation-combined process. This proposal is first evidenced by UV-vis analysis due the suppression of SPR band (Fig. 2b), a behavior consistent with the literature.31,32 Interestingly, TEM analysis clearly shows a growth in AgNPs size after addition of Fe3+ to the solution (Fig. 2d), resulting in a final single AgNPs population with average diameter of 14.7 nm ± 8.9 nm. Due to the strong alkaline medium, the main specie responsible for the behavior of NPs is likely to be Fe(OH)3. A schematic illustration of the mechanism involving aggregation of RU-AgNPs induced by the addition of Fe3+ is presented in Fig. 3. AgNPs are initially formed by adsorption of anionic RU to the silver surface via deprotonated 5-hydroxychromen-4-one moiety. This is supported by the literature33 and confirmed by alteration in the 1800–1500 cm−1 region of the RU infrared spectra before and after coordination with silver (Fig. S9†). Afterwards, the addition of Fe3+ induces the formation of a coordination complex through an anionic catechol group, in which at least 2 : 1 ligand–Fe3+ stoichiometry is required for an aggregated effect.Open in a separate windowFig. 3Mechanistic proposal for growth/aggregation of RU-AgNPs in the presence of Fe3+. Insert: binding model for RU-AgNPs.Due to increasing interest in image processing as an analytical tool for many purposes,34,35 Multiple Linear Regression was employed to verify the capacity of the RU-AgNPs to probe Fe3+ standards at distinct concentrations, as presented in Fig. 4. The curve was obtained by plotting the color absorbances RGB-based values versus the concentrations of Fe3+ standards after RU-AgNPs interaction. Predicted iron is a vector based on RGB values that were then extracted from the filtered images and inserted in the equation described by Beer–Lambert law in order to generate the absorbances for the construction of the analytical curve. A linear behavior between the predicted response and the measured concentrations was observed (R2 = 0.9806).Open in a separate windowFig. 4Calibration curve for Fe3+ analysis showing the predicted iron (RGB) vs. iron(iii) concentration (1 to 8 μmol L−1). Adjusted R2 = 0.9806.Regression coefficients of the calibration model (using the R, G and B channels simultaneously) obtained by MLR method are shown in eqn (1):[Fe3+] = 44.5R − 4.9G − 16.4B + 5.11where Fe3+ concentration is the dependent variable (Predicted Iron), 5.1 is the intercept (β0), 44.5, −4.9 and −16.4 are the regression coefficients of the independent variables (R, G and B channels, respectively).It is possible to observe an interesting performance of the method using the three RGB color channels allied with MLR to quantify the Fe3+ content, which presented reasonable deviations in its responses considering that it is simple and low-cost. The good linearity is similar to other colorimetric methods such as sodium determination in seawater and coconut water (R2 > 0.91) by Moraes and coauthors,36 or iron(ii) in simulated seawater (R2 = 0.9993) by Gasparotto et al.37Second order regression was applied to the dataset to obtain a better adjustment, resulting in an adjusted R-squared of 0.9955. RGB values were then inserted in eqn (2) in order to generate the construction of the correlation:[Fe3+] = 14.4R + 30.7G − 23.4B − 398RG − 348RB − 0.8BG − 978R2 + 695G2 + 26.9B2 + 4.52This paper reports the use of AgNPs functionalized with RU as nanoprobes for selective detection and sensitive quantification of Fe3+ in aqueous solution. The synthesis of RU-AgNPs is reproducible, easily performed and requires no stabilizer agent other than RU. AA has a crucial role in the selectivity by either the avoidance of oxidation of RU by silver and/or coordination of ascorbate with other cations. The literature brings relevant examples of chromogenic and fluorogenic chemosensors for selective detection of Fe3+ in solution. Many of these artificial organic receptors present high selectivity and relevant limits of detection, requiring, however, very specific reagents and laborious synthetic procedures.38–41 Metal-based nanoparticles have emerged as potential probes for detection of Fe3+.11–16 Although effective in Fe3+ sensing, the synthesis of these nanoprobes require some toxic reagents, such as NaBH4 or PVP, use plant extracts, which may lead to some drawbacks, such as the understanding of the sensing mechanism. In contrast, our method is based on commercially available, nontoxic, low-cost reagents. Fe3+ sensing performed satisfactorily in the 1–10 μmol L−1 range, and the limit of detection obtained with this method (17 nmol L−1) is comparable to the most sensitive methods reported in literature. A mechanism for the detection of Fe3+ by RU-AgNPs involves a combined growth/aggregation of the NPs. There is a still limited number of nanoscaled systems reported as being selective and sensitive in the detection of Fe3+, which reinforces the relevance of the method reported herein. The linearity range obtained by both UV-vis spectroscopy and image analysis comprises the maximum of residual Fe3+ in drinking water according to the European and US legislations.15,42 相似文献
26.
Bassim El‐Sabawi Mayra E. Guerrero Mackram F. Eleid Charanjit S. Rihal 《Catheterization and cardiovascular interventions》2020,96(3):706-711
Transcatheter mitral valve replacement (TMVR) is emerging as an alternative treatment strategy to surgery for patients with severe mitral annular calcification (MAC) who are not candidates for traditional mitral valve surgery. Paravalvular leak (PVL) is common following TMVR for severe MAC and can lead to heart failure symptoms and/or intravascular hemolysis, the latter of which usually is clinically stable. We report the case of a 67‐year‐old woman with symptomatic severe aortic stenosis and mitral stenosis with MAC in the setting of prior chest irradiation who was treated initially with transcatheter aortic valve replacement followed by TMVR at a later date (Sapien S3 system; Edwards Lifesciences). Immediately following TMVR, she developed acute profound hemolysis which manifested with hemoglobinuria, transfusion‐dependent anemia, and acute renal failure requiring renal replacement therapy. She was treated with post‐dilation balloon valvuloplasty after failed transcatheter PVL closure 10 days following TMVR with resulting improvement in the PVL. The hemolytic anemia resolved and renal function recovered without the need for continued hemodialysis 2 months later and stabilization of glomerular filtration rate at 6 months. This case highlights a potential severe complication of TMVR in MAC and suggests that improvement in hemolysis and late recovery of renal function may occur following treatment of PVL. 相似文献
27.
Regional odontodysplasia is a rare and significant dental malformation. It is a dental alteration of unknown etiology, involving both mesodermal and ectodermal dental components, which present clinical, radiographic, and histologic features. This article reports a clinical case of a 10-month-old child who was diagnosed with regional odontodysplasia in the maxilla, confirmed by radiographic examination, with a follow-up of 5 years. The clinical, radiographic, and histologic features were reviewed. 相似文献
28.
29.
Rudi Emerson de Lima Procópio Ingrid Reis da Silva Mayra Kassawara Martins João Lúcio de Azevedo Janete Magali de Araújo 《The Brazilian journal of infectious diseases》2012,16(5):466-471
Streptomyces is a genus of Gram-positive bacteria that grows in various environments, and its shape resembles filamentous fungi. The morphological differentiation of Streptomyces involves the formation of a layer of hyphae that can differentiate into a chain of spores. The most interesting property of Streptomyces is the ability to produce bioactive secondary metabolites, such as antifungals, antivirals, antitumorals, anti-hypertensives, immunosuppressants, and especially antibiotics. The production of most antibiotics is species specific, and these secondary metabolites are important for Streptomyces species in order to compete with other microorganisms that come in contact, even within the same genre. Despite the success of the discovery of antibiotics, and advances in the techniques of their production, infectious diseases still remain the second leading cause of death worldwide, and bacterial infections cause approximately 17 million deaths annually, affecting mainly children and the elderly. Self-medication and overuse of antibiotics is another important factor that contributes to resistance, reducing the lifetime of the antibiotic, thus causing the constant need for research and development of new antibiotics. 相似文献
30.
Osvaldo Vilela‐Filho Fernando P. Ferraz Breno A. Barros Luciana O. Silva Suzana F. Anunciação Joaquim T. Souza Delson J. Silva Mauricio B. Leite Ingrid B. Atayde Paulo C. Ragazzo Paula G. Barreto Mariana A. Nobrega Maisa R. Araujo Mayra R. Santos Victor H. Ala Stefano T. Queiroz 《Journal of neuroscience research》2013,91(10):1328-1337
Although long known and the most prevalent movement disorder, pathophysiology of essential tremor (ET) remains controversial. The most accepted hypothesis is that it is caused by a dysfunction of the olivocerebellar system. Vilela Filho et al. [2001; Stereotact Funct Neurosurg 77:149–150], however, reported a patient with unilateral hand ET that was completely relieved after a stroke restricted to the contralateral posterior putamen and suggested that ET could be the clinical manifestation of posterior putamen hyperactivity. The present study was designed to evaluate this hypothesis in the most often used model of ET, harmaline‐induced tremor in rats. Fifty‐four male Wistar rats were randomly distributed into three groups: experimental (EG), surgical control (SCG), and pharmacological control (PCG) groups. EG animals underwent stereotactic unilateral posterior striatotomy. SCG rats underwent sham lesion at the same target. PCG served exclusively as controls for harmaline effects. All animals received, postoperatively, intraperitoneal harmaline, and the induced tremor was video‐recorded for later evaluation by a blind observer. Thirteen animals were excluded from the study. Limb tremor was reduced ipsilaterally to the operation in 20 of 21 rats of EG and in two of nine of SCG, being asymmetric in one of 10 of PCG rats. Comparisons between EG × SCG and EG × PCG were statistically significant, but not between SCG × PCG. Limb tremor reduction was greater in anterior than in posterior paws. Lateral lesions yielded better results than medial lesions. These results suggest that the posterior striatum is involved with harmaline‐induced tremor in rats and support the hypothesis presented. © 2013 Wiley Periodicals, Inc. 相似文献