Absence of the RET+3:T allele in the MTC patients

The mutations of the RET proto-oncogene contributes to the development of MTC by increasing the activity of the receptor encoded by this gene. Variant T of polymorphism rs2435357 located in the enhancer of the RET gene reduces the enhancer’s activity. The opposite effects of rs2435357 and the mutations causing medullary thyroid carcinoma resulted in the investigation of the status of this polymorphism in patients with MTC. In our study, we compared the frequency of polymorphism rs2435357 in the group of 48 MTC patients with its frequency in Polish population. The frequency of heterozygotes C/T at rs2435357 reached almost 12% (18/152) for the Polish population, in contrast to the group of MTC patients where not even a single T allele was found. The frequency difference is statistically significant. This observation might indicate that the presence of the heterozygous T allele at rs2435357 may be associated with the inhibition of medullary thyroid carcinoma development.     

Orbital lymphoma mimicking Graves’ ophthalmopathy: a case report

Orbital tumor is a rare presentation of lymphoma. Unspecific symptoms, local infiltration, chronic progression may mimic other more common orbital diseases and often make the diagnosis difficult. We report a case of orbital non-Hodgkin lymphoma initially diagnosed as Graves’ disease. A 65-year-old woman was admitted to the Department of Endocrinology with a diagnosis of a left eye tumor. On admission, apart from the tumor, exophthalmos and the upper eyelid proptosis were present. The lesion had been observed for two years before hospitalization. Due to the muscle infiltration, as detected on computed tomography scanning and magnetic resonance imaging, Graves’ disease was suggested. The thyroid function was normal. Further diagnosis performed during hospitalization revealed lymphoplasmacytic lymphoma. Lymphoma may manifest as a localized orbital tumor without extraorbital or constitutive symptoms. Rare orbital diseases, among others lymphoproliferations, should be taken into account in the differential diagnosis of exophthalmos.     

Composite osseomusculocutaneous sternum,ribs, thymus,pectoralis muscles,and skin allotransplantation model of bone marrow transplantation

Cellular and vascularized bone marrow cells have been used to induce donor‐specific chimerism in various models of composite tissue allotransplantation. Although thymus transplantation has been reported in the literature, the effect of thymus transplantation on chimerism levels in vascularized bone containing composite tissue allotransplantation has not been reported. In this study, a new method for composite vascularized sternal bone marrow transplant model is descried that can be applied to augment chimerism after transplantation. A total of seven composite osseomusculocutaneous sternum, ribs, thymus, pectoralis muscles, and skin transplantations were performed in two groups. The first group (n = 5) was designed as an allotransplantation group and the second group (n = 2) was designed as an isotransplantation group. Composite osseomusculocutaneous sternum, ribs, thymus, and pectoralis muscles allografts were harvested on thecommon carotid artery and external jugular vein and a heterotopic transplantation was performed to the inguinal region of the recipient rat. Cyclosporine A monotherapy was administered in order to prevent acute and chronic allograft rejection. Animals sacrificed when any sign of rejection occurred. The longest survival was 156 day post‐transplant. Assessment of bone marrow cells within sternum bone component and flow cytometry analysis of donor‐specific chimerism in the peripheral blood of recipients were evaluated. Our results showed that thiscomposite allograft carried 7.5 × 10⁶ of viable hematopoietic cells within the sternum component. At day 7 post‐transplant chimerism was developed in T‐cell population and mean level was assessed at 2.65% for RT1ⁿ/CD4 and at 1.0% for RT1ⁿ/CD8. In this study, a newosseomusculocutaneous sternum, ribs, thymus, pectoralis muscle, and skin allotransplantation model is reported which can be usedto augment hematopoietic activity for chimerism induction after transplantation. © 2012 Wiley Periodicals, Inc. Microsurgery, 2013.     

Laparoscopic Hernia Repair—When Is a Hernia Not a Hernia?

A wide range of diagnoses can present as inguinal hernia. Laparoscopic techniques are being increasingly used in the repair of inguinal hernias and offer the potential benefit of identifying additional pathology. The authors present the first reported case of a hydrocele of the canal of Nuck diagnosed laparoscopically. We review the incidence of identifying additional pathology through laparoscopy for inguinal hernia repair. We suggest that in patients with atypical presenting features of a hernia, the transabdominal preperitoneal, rather than a totally extraperitoneal, approach to groin hernia repair should be considered because of its greater diagnostic potential.     

Oncogenic effects of miR-10b in glioblastoma stem cells

MicroRNAs and cancer stem cells have emerged as critical players in glioblastoma, one of the deadliest human cancers. In this study, we investigated the expression and function of microRNA-10b in glioblastoma cells and stem cells. An analysis of The Cancer Genome Atlas data revealed a correlation between high miR-10b levels and poor prognosis in glioblastoma patients. We measured the levels of miR-10b and found that it is upregulated in human glioblastoma tissues, glioblastoma cell and stem cell lines as compared to normal human tissues or astrocytes. Inhibition of miR-10b with a specific antagomir inhibited the proliferation of glioblastoma established and stem cell lines. Inhibition of miR-10b strongly reduced cell invasion and migration in glioblastoma cell and stem cell lines while overexpression of miR-10b induced cell migration and invasion. We also investigated several predicted targets of miR-10b but could not verify any of them experimentally. Additionally, miR-10b inhibition significantly decreased the in vivo growth of stem cell-derived orthotopic GBM xenografts. Altogether, our findings confirm the oncogenic effects of miR-10b in GBM cells and show for the first time a role of this microRNA in GBM stem cells. Targeting miR-10b might therefore inhibit glioblastoma stem cells, which are thought to be at the origin of glioblastoma and to contribute its recurrence and resistance to therapy.     

Diagnostic and treatment of overactive bladder

The overactive bladder (OAB) is characterized by symptoms of frequency, urgency, nocturia, and urge incontinence, substantially affecting the quality of life of millions of people throughout the world. Diagnosis of OAB made on patient history, physical examination, and clinical tests can be used as the basis for treatment in most cases. In cases where there is uncertainty regarding the diagnosis, urodynamic assessment should be carried out. Treatment of OAB includes behavioral therapy, pharmacology and, in some cases, surgery. This article reviews current findings regarding diagnostics and treatment of the overactive bladder.