全文获取类型
收费全文 | 61158篇 |
免费 | 4009篇 |
国内免费 | 1090篇 |
专业分类
耳鼻咽喉 | 524篇 |
儿科学 | 1577篇 |
妇产科学 | 1565篇 |
基础医学 | 6706篇 |
口腔科学 | 1286篇 |
临床医学 | 5238篇 |
内科学 | 14797篇 |
皮肤病学 | 1006篇 |
神经病学 | 5625篇 |
特种医学 | 2473篇 |
外国民族医学 | 2篇 |
外科学 | 8873篇 |
综合类 | 2154篇 |
现状与发展 | 1篇 |
一般理论 | 5篇 |
预防医学 | 2975篇 |
眼科学 | 773篇 |
药学 | 4760篇 |
8篇 | |
中国医学 | 976篇 |
肿瘤学 | 4933篇 |
出版年
2023年 | 590篇 |
2022年 | 1408篇 |
2021年 | 2270篇 |
2020年 | 1424篇 |
2019年 | 1588篇 |
2018年 | 1822篇 |
2017年 | 1485篇 |
2016年 | 1541篇 |
2015年 | 1900篇 |
2014年 | 2404篇 |
2013年 | 2977篇 |
2012年 | 4057篇 |
2011年 | 4068篇 |
2010年 | 2392篇 |
2009年 | 2224篇 |
2008年 | 3434篇 |
2007年 | 3382篇 |
2006年 | 3097篇 |
2005年 | 2952篇 |
2004年 | 2641篇 |
2003年 | 2287篇 |
2002年 | 2068篇 |
2001年 | 1592篇 |
2000年 | 1523篇 |
1999年 | 1387篇 |
1998年 | 563篇 |
1997年 | 422篇 |
1996年 | 438篇 |
1995年 | 348篇 |
1994年 | 319篇 |
1993年 | 265篇 |
1992年 | 787篇 |
1991年 | 657篇 |
1990年 | 623篇 |
1989年 | 621篇 |
1988年 | 514篇 |
1987年 | 477篇 |
1986年 | 457篇 |
1985年 | 373篇 |
1984年 | 306篇 |
1983年 | 244篇 |
1982年 | 134篇 |
1979年 | 252篇 |
1978年 | 152篇 |
1977年 | 136篇 |
1974年 | 151篇 |
1973年 | 142篇 |
1972年 | 134篇 |
1971年 | 125篇 |
1969年 | 118篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
21.
Jae Eun Choi Tyler Werbel Zhenping Wang Chia Chi Wu Tony L. Yaksh Anna Di Nardo 《Journal of dermatological science》2019,93(1):58-64
Background
Rosacea is a chronic inflammatory skin condition whose etiology has been linked to mast cells and the antimicrobial peptide cathelicidin LL-37. Individuals with refractory disease have demonstrated clinical benefit with periodic injections of onabotulinum toxin, but the mechanism of action is unknown.Objectives
To investigate the molecular mechanism by which botulinum toxin improves rosacea lesions.Methods
Primary human and murine mast cells were pretreated with onabotulinum toxin A or B or control. Mast cell degranulation was evaluated by β-hexosaminidase activity. Expression of botulinum toxin receptor Sv2 was measured by qPCR. The presence of SNAP-25 and VAMP2 was established by immunofluorescence. In vivo rosacea model was established by intradermally injecting LL-37 with or without onabotulinum toxin A pretreatment. Mast cell degranulation was assessed in vivo by histologic counts. Rosacea biomarkers were analyzed by qPCR of mouse skin sections.Results
Onabotulinum toxin A and B inhibited compound 48/80-induced degranulation of both human and murine mast cells. Expression of Sv2 was established in mouse mast cells. Onabotulinum toxin A and B increased cleaved SNAP-25 and decreased VAMP2 staining in mast cells respectively. In mice, injection of onabotulinum toxin A significantly reduced LL-37-induced skin erythema, mast cell degranulation, and mRNA expression of rosacea biomarkers.Conclusions
These findings suggest that onabotulinum toxin reduces rosacea-associated skin inflammation by directly inhibiting mast cell degranulation. Periodic applications of onabotulinum toxin may be an effective therapy for refractory rosacea and deserves further study. 相似文献22.
Devagi Kanakaraju Nurfatyha Rusydah bt Mohamad Shahdad Ying-Chin Lim Andrea Pace 《Sustainable Chemistry and Pharmacy》2019
The use of multifunctional materials for water remediation is a modern approach where adsorption phenomena and heterogeneous photocatalysis can be applied for the removal of pollutants. Since the ideal remediation system should be able to remove both organic and inorganic pollutants, a crucial aspect to consider is the knowledge of operational parameters affecting the removal process, especially when heavy metal ions are present in concoction as in real systems. Given the proven efficiency of multifunctional TiO2/Alg/FeNPs magnetic beads for the removal of model organic pollutants, this study investigated the possibility to exploit such system also for the removal of mixed heavy metals (MHM), specifically Cr(III), Cu(II), and Pb(II) ions, under ultraviolet irradiation at a wavelength of 254 nm. After a preliminary screening on the optimal catalyst loading, operating parameters such as the initial concentration of metal ions, contact and irradiation time, and pH were investigated to optimize the removal of metal ions using response surface methodology (RSM) via Box–Behnken design. Starting from a MHM solution containing 44 ppm of each metal ion, the removal of Pb(II), Cr(III), and Cu(II) ions in the aqueous solution was nearly completed (>98.4%) for all three ions within 72 min of irradiation at almost neutral pH (pH = 6.8). The stability of TiO2/Alg/FeNPs was confirmed by retrieving and reusing the beads in three consecutive cycles of heavy metals removal without observing significant changes in catalyst efficiency. 相似文献
23.
24.
25.
26.
27.
F. Murina R. Felice S. Di Francesco L. Nelvastellio I. Cetin 《Gynecological endocrinology》2020,36(5):431-435
AbstractThis study is a single-center, retrospective analysis of postmenopausal women presenting with dyspareunia and vulvar pain, aiming to evaluate relative effectiveness of vestibular CO2 laser therapy as a treatment. Three monthly sessions of laser were performed to each patient and thereafter a three-months follow-up was stablished. A total number of 72 patients undergoing vestibular laser treatment were recruited from patient files in the period between 2016 and 2018. Among these, 39 women also received a concomitant treatment with ospemifene (60?mg/day) during the study period. There was a statistically significant reduction of all the symptoms in both groups up to the three month follow-up. Regarding dryness and dyspareunia, the relief tent to be more prominent in the ospemifene?+?laser group at all follow-ups and remained statistically significant at three-month follow-up. Specifically, vestibular dryness was significantly lower in the ospemifene?+?laser group compared with the laser treatment group (?87% vs???34%, respectively), and the vestibular health score started declining faster in the ospemifene?+?laser group. Although, additional research is needed to understand the mechanism of action, our data shows that a combination regimen of laser and ospemifene may improve clinical effectiveness for long-term treatment of symptoms associated with the under-recognized genitourinary syndrome of menopause. 相似文献
28.
目的 初步探讨应用艾尔巴韦/格拉瑞韦治疗慢性丙型肝炎(CHC)患者的疗效。方法 2017年3月~2018年3月仙桃市第一人民医院感染病科收治的CHC患者82例,被随机分为对照组41例和观察组41例,分别给予聚乙二醇干扰素-α联合利巴韦林治疗和艾尔巴韦/格拉瑞韦治疗,两组均连续治疗24周。采用RT- PCR法检测血清 HCV RNA,采用全基因序列测定法行病毒基因分型。比较两组早期病毒学应答(EVR)、治疗结束时病毒学应答(ETVR)和持续病毒学应答(SVR)。结果 在治疗结束时,观察组血清丙氨酸氨基转移酶(ALT)水平为(47.9±19.7)U/L,显著低于对照组【(63.5±21.2)U/L,P<0.05】,天冬氨酸氨基转移酶(AST)水平为(55.5±22.3)U/L,显著低于对照组【(81.3±25.8)U/L,P<0.05】;观察组EVR、ETVR和SVR分别为48.8%、63.4%和70.7%,与对照组的41.5%、53.7%和65.8%比,无统计学差异(P>0.05);18例观察组非HCV Ⅰ型感染者EVR、ETVR和SVR分别为88.9%、94.4%和88.9%,显著高于同组23例HCV Ⅰ型感染者(分别为52.2%、60.9%和52.2%, P<0.05),而与对照组15例非HCV Ⅰ型感染者比,无统计学差异(分别为86.7%、93.3%和73.3%, P>0.05);观察组SVR12为87.8%(36/41),显著高于对照组的73.2%(30/41,P<0.05)。结论 应用直接抗病毒(DAA)药物艾尔巴韦/格拉瑞韦治疗CHC患者近期疗效达到,但远期疗效似优于标准治疗方案, 值得临床进一步验证。 相似文献
29.
30.
Psychological characteristics and physiological reactivity to acute stress in mothers of children with autism spectrum disorder 总被引:1,自引:0,他引:1
Elena Pattini Luca Carnevali Alfonso Troisi Guido Matrella Dolores Rollo Mauro Fornari Andrea Sgoifo 《Stress and health》2019,35(4):421-431
Stress related to parenting a child with autism spectrum disorder can differently affect caregiver's physiological reactivity to acute stress. Here, parental stress levels, psychological characteristics, and coping strategies were assessed alongside measures of heart rate, heart rate variability, and cortisol during a psychosocial stress test in mothers of children with ASD (M‐ASD, n = 15) and mothers of typically developing children (n = 15). M‐ASD reported significantly higher levels of parental stress, anxiety, negative affectivity, social inhibition, and a larger preference for avoidance strategies. M‐ASD showed larger heart rate and cortisol responses to the psychosocial stress test. A positive relationship was found between parental stress levels and the magnitude of the cortisol stress response in both groups. The present findings indicate exaggerated physiological reactivity to acute psychosocial stress in M‐ASD and prompt further research to explore the role of individual differences in mediating the effects of parental stress on physiological stress responses. 相似文献