Omega-3 fatty acid treatment fails to alter alpha 1-adrenergic receptor mediated phosphoinositide turnover in streptozotocin-induced diabetic rats

1. The effect of omega-3 fatty acid supplementation on alpha 1-adrenergic receptor-mediated phosphoinositide turnover in the hearts of 6-week streptozotocin diabetic rats was studied. 2. The diabetic state was characterized by decreased body weight gain, hypoinsulinemia and hyperglycemia. Omega-3 fatty acid treatment did not have any significant effects on the above parameters. 3. Plasma triglyceride and cholesterol levels were significantly higher in diabetic rats compared with controls. 4. Omega-3 fatty acid treatment reduced the plasma triglyceride levels in the diabetic rats, whereas plasma cholesterol levels remained unaffected. 5. Norepinephrine (10 and 100 microM) stimulated inositol monophosphate (IP1) formation was significantly decreased in the diabetic rat heart compared with controls. Omega-3 fatty acid treatment had no significant effect on the norepinephrine-mediated IP1 formation in these rats. 6. Inositol bisphosphate (IP2) formation in response to 100 microM of norepinephrine was significantly lower in the diabetic rat heart; which was also not affected by omega-3 fatty acid treatment.     

大鼠头颅瞬间侧向旋转后脑干水肿的形成及其超微形态学基础

目的：探讨头颅瞬间旋转后脑干水肿的形成及超微形态学基础。     

抗击"非典"为改善医患关系提供了新契机

文章通过分析在抗击“非典”特殊时期医务人员为科学、为病人的献身精神，以及广大人民对医疗高风险、高费用的全面了解，论证抗击“非典”为病人重新认识医务人员，促进医患沟通，改善日趋紧张的医患关系提供了新契机。     

新药甲磺酸帕珠沙星的手性拆分

目的：建立新药甲磺酸帕珠沙星手性拆分的方法。方法：运用AD手性柱法和流动相添加剂法，考察了不同条件下对分离的影响。结果：运用这两种方法，甲磺酸帕珠沙星都可以达到基线分离，相同条件下，柱温越低，拆分因子越大。结论：在分离甲磺酸帕珠沙星时，流动相添加剂法要优于手性柱法。     

肝移植手术中的配合

自从 1 96 3年Starzl成功实施世界上第一例活体肝移植后 ,肝移植手术已经成为现代肝脏外科学领域里的一个比较实用、也比较时髦的手术及研究项目。结合我院已完成的 2例肝移植手术 ,简要介绍肝移植手术术前、术中及术后的相关问题 ,并介绍肝移植手术的主要步骤 ,叙述手术中的相关配合问题     

毛喉萜对人肝癌细胞增殖影响的实验研究

目的　探讨毛喉萜 (Forskolin)的抗肝癌作用。方法　采用克隆形成、MTT比色法观察毛喉萜对SMMC 772 1细胞的作用 ,并利用免疫细胞化学方法检测其对rasp2 1、p5 3蛋白表达的影响。结果　毛喉萜可明显抑制SMMC 772 1细胞的增殖 ,其抑制率与剂量呈正相关 (P <0 .0 1 ) ,经毛喉萜处理后 ,rasp2 1、p5 3蛋白表达均有明显下降。结论　毛喉萜能明显抑制肝癌SMMC 772 1细胞增殖 ,其作用可能与降低rasp2 1、p5 3蛋白表达有关。     

医学影像存储的色彩模式和文件格式的比较

本文介绍了不同的影像色彩模式和影像文件常见存储格式的特点及其在应用上的优缺点，分析了影像色彩模式、文件存储的格式与影像质量、用途的关系。     

雷帕霉素对人肝癌裸鼠原位移植瘤生长的抑制作用

目的探讨雷帕霉素对人肝癌裸鼠原位移植瘤生长的影响及其机制。方法建立人肝癌棵鼠肝原位移植瘤模型32只,随机分为空白对照组、FK506组、雷帕霉素常规剂量组和高剂量组。用药两周后观察肿瘤体积的变化,免疫组织化学法检测肝癌组织中增殖细胞核抗原(PCNA)、血管内皮细胞生长因子(VEGF)的表达。结果对照组、FK506组、雷帕霉素常规剂量组、高剂量组平均肿瘤体积分别为:(310．15±40．16)、(605．59±116．23)、(99．19±15．27)、(151．61±27．81) mm3。与对照组相比,雷帕常规剂量组及高剂量组肿瘤体积明显缩小,PCNA、VEGF的表达均显著下调(P<0．01);FKS06组肿瘤体积与对照组相比明显增大,差异有统计学意义(P<0．01),PC- NA、VEGF的表达与对照组相比差异无统计学意义(P>0．01)。结论雷帕霉素具有显著抑制肝癌生长的作用,其机制可能是抑制了肝癌细胞的恶性增殖及VEGF的产生。     

明党参根中维生素及其水煎膏中无机元素的分析

目的:考察明党参根中营养元素的种类及含量,探讨其补益功效的物质基础.方法:采用高效液相色谱法、荧光分光光度法及扫描电子微量分析法,测定明党参根中维生素的含量和水煎膏中无机元素的含量.结果:明党参根含脂溶性维生素E、K分别为259.2、64.1μg/kg,水溶性维生素C、B1、B2分别为78.5、0.6、0.7 mg/kg.明党参水煎膏中含丰富的钾和钙离子.结论:明党参富含人体必需的有益元素,作为食用中药是有其物质基础的.     

Studies on the chemical constituents of the roots of Rhododendron molle G. don

Summary The compounds from the root ofRhododendron molle G. Don were isolated, purified by various chromatographic techniques, and their structures were identified according to the physical and chemical features and spectral data. Three compounds were separated from the root ofRhododendron molle G. Don and identified as Rhodojaponin-III,taraxerol, β-sitosterol for the first time. XIANG Yanni, female, born in 1976, Postgraduate student