964天冷冻保存的马来微丝蚴的生物学特性

参照Ham等的二步冷冻法,在含有5％DMSO和20％小牛血清的台氏液中,马来微丝蚴冷冻保存(-196℃)964天,复苏后仍具有活力,其平均存活率达94.2％,平均正常外貌达71.4％,平均活动等级达2.45。对中华按蚊的感染率为18.4％,感染度为2.36条/只。     

改制先改革,改革先改人宿迁医改的政府内应力

“人民,是创造历史的真正动力”——壮哉斯言；“真理,只掌握在少数人手中”——诚哉斯言。尽管发生在苏北宿迁的这场席卷城乡的改革,绝不可能只由少数精英分子独立完成,但最初的推动力,是全民意志的厚积薄发?是少数精英的灵光一闪?抑或是一场上级授权、政府定规、全民参与的社会实验?     

胸部类癌

本文报道了３１例胸部类癌，其中胸腺类癌１４例，支气管类癌１７例，伴有异位ＡＣＴＨ综合征者１７例。１２例患者发生转移（３９％），２４例行原发肿瘤全部或部分切除，术后放疗或化疗或两者均有者１７例，４例仅行探查并取活检，２８例经手术及病理证实。     

小儿肾积水Anderson－Hynes手术后并发症及其治疗

对５２例（５４侧）先天性肾盂输尿管连接部梗阻性肾积水患者行Ａｎｄｅｒｓｏｎ－Ｈｙｎｅｓ手术，对其手术的并发症进行分析，主要并发症为吻合口梗阻（３９％）和泌尿系感染（２８％）．经腹进行手术，肠梗阻发生率为１３％（３／２３）．患儿手术时年龄与手术后并发症发生率无显著性意义．     

膀胱癌组织形态定量分析和病理学研究：附20例报告

对２０例膀胱移行上皮癌大切片ＨＥ染色，观察肿瘤表面、基部，浸润深部的组织形态特征，运用ＡｇＮＯＲ银染技术和图像分析技术进行了ＡｇＮＯＲ颗粒计数和核形态计量研究。结果表明：膀胱移行上皮癌表面组织与基部组织的分级和恶性程度无显著性差异。肿瘤浸润深部的组织分级与恶性程度比肿瘤表面组织的高。     

反相高效液相色谱法测定洛氟沙星的血药浓度

采用反相ＨＰＬＣ法测定洛氟沙星的血药浓度，血浆样品用二氯甲烷在ｐＨ７．０条件下提取后进样，流动相为１０ｍ ｍｏｌ／Ｌ磷酸二氢钾－１０ｍ ｍｏｌ／Ｌ溴化四丁铵－乙腈－三乙胺（４５：４４：１０：１，磷酸调节ｐＨ２．８），紫外检测器λ＝２９５ｎｍ，最低检测浓度１０μｇ／Ｌ，线性范围：０．１￣６．０ｍｇ／Ｌ，ｒ＝０．９９９９，日内ＲＳＤ为１．３８％￣３．４２％，日间ＲＳＤ为１．１１％￣２．８９％。     

Cannabinoid receptor down-regulation without alteration of the inhibitory effect of CP 55,940 on adenylyl cyclase in the cerebellum of CP 55,940-tolerant mice

The objective of this study was to determine whether the development of tolerance to CP 55,940, a potent cannabinoid agonist, was due to changes in the receptor or second messenger system. ICR mice treated with CP 55,940 (2 mg/kg) twice a day for 6 and one-half days developed a high degree of tolerance to the pharmacological effects of CP 55,940. The ability of CP 55,940 to produce motor hypoactivity, hypothermia and immobility was reduced 163-, 97- and 19-fold, respectively. Evaluation of 3H-CP 55,940 binding to rat brain membranes indicated no difference in receptor affinity between the vehicle- and CP 55,940-treated animals. However, these binding studies revealed a 50% decrease in receptor number in the cerebellum of the CP 55,940-tolerant mice. Although cAMP is generally considered to be the second messenger for cannabinoid receptors, little difference was observed in the inhibitory effects of CP 55,940 on adenylyl cyclase activity in cerebellum between vehicle and drug-treated mice. However, there was an increase in receptor mRNA which suggests a compensation for receptor loss. There are several possible explanations for these results. There may be sufficient spare receptors such that CP 55,940-tolerant mice are capable of producing a maximal effect on the second messenger system. On the other hand, one could conclude that cannabinoid receptor down-regulation does not account for the development of tolerance to all of the effects of CP 55,940 in mice.     

10Gyγ线照射后小鼠两相邻肠段运动变化

本文用同时记录两相邻空肠段的收缩活动来观察经10Gyγ线照射后小肠运动的变化,以了解照射后是否出现逆蠕动,以便进一步分析肠套叠形成的原因.结果表明,照射后两相邻肠段出现强度不一的收缩活动,并且可以产生逆蠕动;但是这种逆蠕动发生的机率是不大的.本实验未观察到逆蠕动发生的规律性.因此,逆蠕动的产生可能是照射后发生肠套叠的部分原因.     

股骨颈骨折粗细双螺钉内固定生物力学研究

股骨颈骨折内固定方法繁多。本文采用一粗一细加压螺纹钉，采用先进电阻应变仪方法，对股骨颈的应力分布规律在中立、外展、内收不同位置上施加50kg荷载压力．通过电阻应变计测定一耗一细两钉所承受拉、压应力优于其它内固定形式。     

胼胝体发育不全的MRI表现

对６例胼胝体发育不全的ＭＲＩ表现进行了分析。矢位像可清楚显示胼胝体的形态学变化，冠位或矢位像较轴拉像更易确认三脑室的移位。ＭＲＩ的多方向成像和高组织分辩率较ＣＴ更有利于对胼胝体发育不全及所合并的其他中枢神经系统异常的诊断，故可做为首选的影像学检查方法。