全文获取类型
收费全文 | 5547篇 |
免费 | 290篇 |
国内免费 | 10篇 |
专业分类
耳鼻咽喉 | 48篇 |
儿科学 | 184篇 |
妇产科学 | 181篇 |
基础医学 | 791篇 |
口腔科学 | 161篇 |
临床医学 | 453篇 |
内科学 | 1168篇 |
皮肤病学 | 89篇 |
神经病学 | 416篇 |
特种医学 | 166篇 |
外科学 | 716篇 |
综合类 | 20篇 |
一般理论 | 2篇 |
预防医学 | 348篇 |
眼科学 | 37篇 |
药学 | 373篇 |
中国医学 | 10篇 |
肿瘤学 | 684篇 |
出版年
2023年 | 32篇 |
2022年 | 91篇 |
2021年 | 137篇 |
2020年 | 65篇 |
2019年 | 133篇 |
2018年 | 157篇 |
2017年 | 88篇 |
2016年 | 98篇 |
2015年 | 135篇 |
2014年 | 169篇 |
2013年 | 256篇 |
2012年 | 364篇 |
2011年 | 380篇 |
2010年 | 202篇 |
2009年 | 202篇 |
2008年 | 317篇 |
2007年 | 320篇 |
2006年 | 285篇 |
2005年 | 306篇 |
2004年 | 286篇 |
2003年 | 268篇 |
2002年 | 173篇 |
2001年 | 108篇 |
2000年 | 140篇 |
1999年 | 139篇 |
1998年 | 62篇 |
1997年 | 39篇 |
1996年 | 28篇 |
1995年 | 27篇 |
1994年 | 30篇 |
1993年 | 24篇 |
1992年 | 68篇 |
1991年 | 70篇 |
1990年 | 66篇 |
1989年 | 61篇 |
1988年 | 61篇 |
1987年 | 63篇 |
1986年 | 62篇 |
1985年 | 56篇 |
1984年 | 43篇 |
1983年 | 39篇 |
1982年 | 16篇 |
1981年 | 22篇 |
1980年 | 14篇 |
1979年 | 30篇 |
1978年 | 20篇 |
1976年 | 10篇 |
1975年 | 15篇 |
1973年 | 13篇 |
1972年 | 8篇 |
排序方式: 共有5847条查询结果,搜索用时 15 毫秒
81.
Xerophilic fungal species of the genus Aspergillus are economically highly relevant due to their ability to grow on low water activity substrates causing spoilage of stored goods and animal feeds. These fungi can synthesize a variety of secondary metabolites, many of which show animal toxicity, creating a health risk for food production animals and to humans as final consumers, respectively. Animal feeds used for rabbit, chinchilla and rainbow trout production in Argentina were analysed for the presence of xerophilic Aspergillus section Aspergillus species. High isolation frequencies (>60%) were detected in all the studied rabbit and chinchilla feeds, while the rainbow trout feeds showed lower fungal charge (25%). These section Aspergillus contaminations comprised predominantly five taxa. Twenty isolates were subjected to taxonomic characterization using both ascospore SEM micromorphology and two independent DNA loci sequencing. The secondary metabolite profiles of the isolates were determined qualitatively by HPLC-MS. All the isolates produced neoechinulin A, 17 isolates were positive for cladosporin and echinulin, and 18 were positive for neoechinulin B. Physcion and preechinulin were detected in a minor proportion of the isolates. This is the first report describing the detailed species composition and the secondary metabolite profiles of Aspergillus section Aspergillus contaminating animal feeds. 相似文献
82.
Fernanda Neves de Carvalho Carolina Toniolo Zenatti Danila Cassia Reis Santana Juliana Maria da Silva Juliane Cristina Oliveira dos As Katia Kisielow dos Anjos Jessica Sigari Cassia de Lima Santos Marcelle Guerra Olivia Pereira Barros Tomaz Cochemore Roberto Camargo Narciso Mario Lucio Baptista Filho 《The Brazilian journal of infectious diseases》2018
83.
Antonio Greco Mariaenrica Frigione Alfonso Maffezzoli Alessandro Marseglia Alessandra Passaro 《Materials》2014,7(7):5385-5402
This work is aimed to present an innovative technology for the reinforcement of beams for urban furniture, produced by in-mold extrusion of plastics from solid urban waste. This material, which is usually referred to as “recycled plastic lumber”, is characterized by very poor mechanical properties, which results in high deflections under flexural loads, particularly under creep conditions. The Prowaste project, founded by the EACI (European Agency for Competitiveness and Innovation) in the framework of the Eco-Innovation measure, was finalized to develop an innovative technology for selective reinforcement of recycled plastic lumber. Selective reinforcement was carried out by the addition of pultruded glass rods in specific positions with respect to the cross section of the beam, which allowed optimizing the reinforcing efficiency. The reinforcement of the plastic lumber beams with pultruded rods was tested at industrial scale plant, at Solteco SL (Alfaro, Spain). The beams obtained, characterized by low cost and weight, were commercialized by the Spanish company. The present paper presents the most relevant results of the Prowaste project. Initially, an evaluation of the different materials candidates for the reinforcement of recycled plastic lumber is presented. Plastic lumber beams produced in the industrial plant were characterized in terms of flexural properties. The results obtained are interpreted by means of beam theory, which allows for extrapolation of the characteristic features of beams produced by different reinforcing elements. Finally, a theoretical comparison with other approaches which can be used for the reinforcement of plastic lumber is presented, highlighting that, among others, the Prowaste concept maximizes the stiffening efficiency, allowing to significantly reduce the weight of the components. 相似文献
84.
C. Angelini C. Semplicini S. Ravaglia B. Bembi S. Servidei M. Moggio M. Filosto E. Sette E. Pegoraro G. Crescimanno P. Tonin R. Parini L. Morandi G. Marrosu G. Greco O. Musumeci G. Di Iorio G. Siciliano M.A. Donati T. Mongini A. Toscano L. Vercelli R. Di Giacopo V. Lucchini V. Tugnoli M. Rigoldi R. Piras F. Giannini S. Gasperini L. Volpi D. Diodato A. Ariatti 《Acta myologica》2011,30(2):152
85.
Katia Herz Alexandra Becker Chenyue Shi Masatsugo Ema Satoru Takahashi Michael Potente Michael Hesse Bernd K. Fleischmann Daniela Wenzel 《Angiogenesis》2018,21(2):349-361
Endothelial cell proliferation is a key process during vascular growth but its kinetics could only be assessed in vitro or ex vivo so far. To enable the monitoring and quantification of cell cycle kinetics in vivo, we have generated transgenic mice expressing an eGFP-anillin construct under control of the endothelial-specific Flt-1 promoter. This construct labels the nuclei of endothelial cells in late G1, S and G2 phase and changes its localization during the different stages of M phase, thereby enabling the monitoring of EC proliferation and cytokinesis. In Flt-1/eGFP-anillin mice, we found eGFP+ signals specifically in Ki67+/PECAM+ endothelial cells during vascular development. Quantification using this cell cycle reporter in embryos revealed a decline in endothelial cell proliferation between E9.5 to E12.5. By time-lapse microscopy, we determined the length of different cell cycle phases in embryonic endothelial cells in vivo and found a M phase duration of about 80 min with 2/3 covering karyokinesis and 1/3 cytokinesis. Thus, we have generated a versatile transgenic system for the accurate assessment of endothelial cell cycle dynamics in vitro and in vivo. 相似文献
86.
A. Greco A. Gallo M. Fusconi G. Magliulo R. Turchetta C. Marinelli G.F. Macri A. De Virgilio M. de Vincentiis 《Autoimmunity reviews》2013,12(3):396-400
ObjectivesThe objective of our study was to review our current knowledge of the aetiopathogenesis of Cogan's syndrome, including viral infection and autoimmunity, and to discuss disease pathogenesis with relevance to pharmacotherapy.Systematic review methodologyRelevant publications on the aetiopathogenesis and pharmacotherapy of Cogan's syndrome from 1945 to 2012 were analysed.Results and conclusionsCogan's syndrome is a rare autoimmune vasculitis, and its pathogenesis is unknown. Infection, but primarily autoimmunity, may play contributing roles in the pathogenesis of this disease. It is characterised by ocular and audiovestibular symptoms similar to those of Meniere's syndrome. Approximately 70% of patients have systemic disease, of which vasculitis is considered the pathological mechanism. The immunologic theory is based on the release of auto-antibodies against corneal, inner ear and endothelial antigens, and of anti-nuclear cytoplasmic auto-antibodies (ANCA).Corticosteroids are the first line of treatment, and multiple immunosuppressive drugs have been tried with varying degrees of success. Tumour necrosis factor (TNF)-alpha blockers are a category of immunosuppressive agents representing a recent novel therapeutic option in Cogan's syndrome. 相似文献
87.
88.
89.
Gonzalo Jr Recondo Enrique Dìaz Canton Màximo de la Vega Martin Greco Gonzalo Sr Recondo Matias E Valsecchi 《World journal of clinical oncology》2014,5(3):440-454
During the last 15 years we have witnessed an unprecedented expansion in the drugs developed to target human epidermal growth factor receptor-2 (HER-2) positive breast cancer. Trastuzumab, pertuzumab, ado-trastuzumab emtansine and lapatinib are currently food and drug administration (FDA)-approved for the treatment of breast cancer patients with HER-2 over-expressed. However, given the amount of information gathered from years of uninterrupted clinical research, it is essential to have periodic updates that succinctly recapitulate what we have learnt over these last years and help us to apply that information in our daily practice. This review will pursue that objective. We will summarize the most relevant and updated information related to the state of the art management of HER-2 positive breast cancer in all the clinical scenarios including the adjuvant, neoadjuvant and metastatic settings. But we will also critically appraise that literature in order to highlight some key clinical concepts that should not be overlooked. Lastly, this review will also point out some of the most promising strategies that are currently being tested and may soon become available. 相似文献
90.
Priccila Zuchinali Gabriela C. Souza Graziella Aliti Mariana R. Botton Lívia Goldraich Katia G. Santos Mara H. Hutz Eliane Bandinelli Luis E. Rohde 《Journal of thrombosis and thrombolysis》2014,37(3):338-344
Significant inter-individual variability on the effect of vitamin K to reverse overanticoagulation has been identified. Genetic polymorphisms of the vitamin K epoxide reductase complex subunit 1 (VKORC1) gene might explain in part this variability. The objective of this study was to evaluate the influence of VKORC1 ?1639G>A and 3730G>A polymorphisms on the effect of oral vitamin K supplementation in overanticoagulated patients. We performed an interventional trial of oral vitamin K supplementation in over-anticoagulated outpatients (international normalized ratio [INR] ≥ 4). Subjects received vitamin K (2.5–5.0 mg) according to baseline INR and were genotyped by real time polymerase chain reaction (PCR). INR values were determined at 3, 6, 24 and 72 h after supplementation. We evaluated 33 outpatients, 61 % were males, with a mean age of 62 ± 12 years old. There was a significant decrease in INR values over time for both polymorphisms after oral vitamin K. At 3 h after supplementation, patients carrying the G allele for the ?1639G>A polymorphism had a greater decrease in INR values compared to AA patients (p < 0.05 for difference among groups; p < 0.001 for time variation; p = 0.001 for time × group interaction), with differences of ?1.01 for GG versus AA (p = 0.003) and ?0.84 for GA versus AA (p = 0.024). Mean INR value at 24 h was 1.9 ± 0.6 and at 72 h was 2.1 ± 0.7, with no differences among genotypes. No significant interaction was identified between the 3730G>A polymorphism and vitamin K supplementation. Our study indicated that the VKORC1 ?1639G>A polymorphism plays a role in the response to acute vitamin K supplementation in over-anticoagulated patients, with faster decrease of INR value in patients carrying the G allele. 相似文献