Complete remission after autografting for chronic myeloid leukaemia

A previously untreated 31-yr old female with Ph-positive chronic myeloid leukaemia (CML) received busulphan and melphalan at high dosage followed by an autograft of peripheral blood stem cells collected 4 weeks earlier. Though recovering haemopoiesis was at first mainly Ph-positive, Ph-negative haemopoiesis predominated at 12 months and has persisted until the most recent study (24 months post-autograft). Her haemopoietic cells now show no rearrangement of the bcr gene, unlike the cells collected at diagnosis. Autografting carried out soon after diagnosis could be valuable for obtaining cytogenetic remissions in other patients with CML.     

人卵巢癌裸鼠移植腹水瘤模型的建立

建立人卵巢癌裸鼠移植腹水瘤模型,为研究人卵巢癌发病机制,生物学特性以及转移方式提供工具。方法将１例人卵巢癌的腹水直接接种于裸鼠腹腔,观察裸鼠腹水形以及肿瘤生长和转移情况,做腹水细胞学计数、涂片以及染色体和肿瘤标志物的检查,肿瘤和器官作病理学检查。结果成功建成１个人卵巢癌裸鼠移植腹水瘤模型,并已传至第１８代,传代移植成功率１００．０％,组织学和超微结构结构形态证实,保持了原人卵巢癌特征,具有人类肿瘤     

替吉奥或卡培他滨联合奥沙利铂治疗晚期胃癌效果比较

目的：对比替吉奥联合奥沙利铂（L-OHP）与卡培他滨联合L-OHP治疗晚期胃癌的疗效和不良反应。方法对2011年3月至2014年12月郑州人民医院收治的93例晚期胃癌患者资料进行回顾性分析。其中替吉奥联合L-OHP治疗组（SL组）48例,卡培他滨联合L-OHP治疗组（XL组）45例。SL组：给予替吉奥每天80 mg／m2,分两次口服,第1天至第14天;L-OHP 130 mg／m2,静脉滴注2 h,第1天。XL组：卡培他滨每天2000 mg／m2,分两次口服,第1天至第14天;L-OHP 130 mg／m2,静脉滴注2 h,第1天。化疗周期为21 d。完成2个周期后评估疗效和不良反应。结果SL组和XL组总有效率分别为52.08%（25／48）和53.33%（24／45）,差异无统计学意义（P＞0.05）。SL组消化道不良反应发生率高于XL组[52.08%（25／48）和24.44%（11／45）,P＜0.05]。SL组口腔黏膜炎发生率低于XL组[25.00%（12／48）和51.11%（23／45）,P <0.05]。结论替吉奥联合L-OHP与卡培他滨联合L-OHP治疗晚期胃癌均安全、有效。     

伊达比星加中剂量阿糖胞苷强化疗对急性髓系白血病预后的影响

目的：观察伊达比星加中剂量阿糖胞苷强化疗治疗急性髓系白血病（AML）的疗效和安全性。方法以北京军区总医院血液科2010年1月至2015年1月应用中剂量阿糖胞苷（2 g／m2,1次／12 h,第1天至第3天）联合伊达比星（12 mg／m2第1天）强化巩固治疗的50例诱导化疗缓解的 AML 患者为试验组,选择同期单药阿糖胞苷（2 g／m2,1次／12 h,第1天至第3天）治疗的50例患者为对照组。两组患者均序贯治疗6个疗程,试验组男性28例,女性22例,平均年龄27.6岁（18～52岁）,原发病包括M1型3例,M2型27例,M4型8例,M5型12例;对照组男性30例,女性20例,平均年龄26.8岁（16～50岁）,原发病包括 M1型2例,M2型30例,M4型8例,M5型10例。观察两组患者化疗并发症和无病生存等情况。结果随访至2015年6月,试验组1例死于肺部感染,2例死于感染性休克;对照组3例死于肺部感染,1例死于感染性休克。试验组和对照组患者治疗相关死亡率分别为6%（3／50）、8%（4／50）（P＞0.05）,复发率分别为32%（16／50）、52%（26／50）（P＜0.05）,无病生存率分别为62%（31／50）、40%（20／50）（P＜0.05）。结论伊达比星加中剂量阿糖胞苷强化疗治疗 AML 与单药阿糖胞苷相比复发率降低,无病生存率显著提高,且治疗相关死亡未增加,值得临床进一步应用。     

HSV1—TK转染人肺腺癌细胞A549的实验研究

目的体内外观察HSV     

微波固化保肢术后成骨肉瘤患者红细胞免疫功能的变化

研究微波固化保肢术提高患者生存率的免疫学机制。方法应用色散比浊法检测患者血清Ｃ３、Ｃ４含量,应用红细胞Ｃ３ｂ受体花环、免疫复合物花环及直接肿瘤花环检测红细胞免疫功能。     

Structural basis for alpha fetoprotein‐mediated inhibition of caspase‐3 activity in hepatocellular carcinoma cells

Alpha‐fetoprotein (AFP) is an early serum growth factor in the foetal liver development and hepatic carcinogenesis; However, the precise biological role of cytoplasmic AFP remains elusive. Although we recently demonstrated that cytoplasmic AFP might interact with caspase‐3 and inhibit the signal transduction of apoptosis in human hepatocellular carcinoma (HCC) cells, the details of this interaction are not clear. To reveal the molecular relationship between AFP and caspase‐3, we performed molecular docking, co‐immunoprecipitation (Co‐IP), laser confocal microscopy, site‐directed mutagenesis and functional experiments to analyse the key amino acid residues in the binding site of caspase‐3. The results of Co‐IP, laser confocal microscopy and functional analyses were consistent with the computational model. We also used the model to explain why AFP cannot bind to caspase‐8. These results provide the molecular basis for the AFP‐mediated inhibition of caspase‐3 activity in HCC cells. Altogether, we found that AFP interacts with caspase‐3 through precise amino acids, namely loop‐4 residues Glu‐248, Asp‐253 and His‐257. The results further demonstrated that AFP plays a critical role in the inhibition of the apoptotic signal transduction that mediated by caspase‐3. Thus, AFP might represent a novel biotarget for the therapy of HCC patients.     

血清白蛋白水平与食管癌再程放疗预后关系的研究

目的:评估血清白蛋白水平在预测食管癌再程放疗预后中的价值.方法:收集病理学确诊的需要接受再程放疗的局部复发食管癌患者,放疗前行血清白蛋白检测,采用Kaplan-Meier法和Log-rank检验进行生存率计算,Cox回归模型分析预后影响因素.结果:血清白蛋白水平与分期有关(P=0.002),差异具有统计学意义(P ＜0.05);62例患者血清白蛋白高值组(≥35 g/L)的1、3、5年总生存率均高于低值组(＜35 g/L)(P =0.001),高值组的6个月和12个月的复发后生存率均高于低值组(P =0.026),以上差异均具有统计学意义(P＜0.05);血清白蛋白水平和复发时间是影响总生存时间的独立预后因素(P =0.015和P=0.000),差异具有统计学意义(P＜0.05).结论:食管癌再程放疗前行血清白蛋白检测对预测患者预后具有重要意义.     

抑癌基因PTEN依赖其磷酸活性诱导人膀胱癌细胞BIU-87失巢凋亡机制

Objective： To investigate the effects and mechanisms of tumor suppressor gene PTEN on the induction of anoikis of human bladder transitional carcinoma cells BIU-87. Methods： BIU-87 cells were transfected with GFP plasmids containing wild-type PTEN or phosphatase inactivating mutant PTEN （C124A-PTEN） in vitro. The PTEN expression and the phosphorylation levels of focal adhesion kinase （FAK） and protein kinase B （PKB/Akt） were detected by Western blotting. Flow cytometry assay and laser scanning confocal microscopy were used to analyze apoptosis in adherent and non-adherent cells. Results： Compared with the control group; PTEN expression in the cells transfected with wild-type PTEN increased to 210%-260%, while the phosphorylation level of FAK and Akt decreased 59% （ P 〈 0.01） and 89% （ P 〈 0.01）, respectively. And the anoikis percentage increased from 8,32 ± 0.57% to 37.62 ± 2.12%, In the cells transfected with C124A-PTEN, neither the phosphorylation of FAK and Akt nor the anoikis percentage had obviously changed, although the PTEN expression enhanced remarkably in comparison with the control. Conclusion： Through its phosphatase activity, tumor suppressor gene PTEN can suppress the phosphorylation of FAK and Akt, and induce anoikis in human bladder transitional carcinoma cells BIU-87.     

X线全身照射与低剂量环磷酰胺合用对实验性肺转移的抑制作用

７５ｍＧｙ一次全身Ｘ线照射后，小鼠实验性肺肿瘤转移结节明显减少；与低剂量环磷酰胺（ＣＴＸ）合用，抗肿瘤转移作用亦较强。荷瘤小鼠７５ｍＧｙ照射后，脾脏ＮＫ细胞活性、ＬＡＫ细胞活性以及对白细胞介素Ⅱ（ＩＬ－２）的反应性明显增强或升高；与ＣＴＸ合用时上述抗肿瘤免疫功能也增强。结果表明：７５ｍＧｙＸ线全身照射可通过激活体内抗肿瘤免疫而具有有效的抗转移效果，它为探讨小剂量照射临床应用可能性提供了可靠的实验依据。