恶性肿瘤患者血清癌胚抗原放射免疫分析

本文采用放射免疫法测定了144例恶性肿瘤患者血清CEA含量,并以同样条件测定了30例正常人作为对照。结果表明恶性肿瘤患者血清CEA含量显著高于健康人,均值为10.3±4.84ng/ml。不同肿瘤患者血清CEA升高的程度不等,其中以肺癌含量最高为14.7±7.83ng/ml。转移性肿瘤CEA含量高于144例肿瘤的平均水平。术后复发者含量不见下降。癌性胸腹水中CEA含量明显升高。因此,血清CEA的测定对恶性肿瘤的辅助诊断,评价疗效和判断预后具有重要的临床意义。     

A quick, sensitive high-performance liquid chromatography assay for monoethylglycinexylidide and lignocaine in serum/plasma using solid-phase extraction.

Recent advances in the investigation of liver disease and transplantation have seen the introduction of lignocaine as a probe of liver function. For this purpose, an assay that is sensitive and rapid is required for the major metabolite of lignocaine, monoethylglycinexylidide (MEGX). We have developed an accurate, low-cost high-performance liquid chromatography (HPLC) method using Bond-Elut phenyl (1 cc) cartridges for sample preparation. The total preparation time for five samples is less than 10 min and the run time is approximately 10 min/sample. Each cartridge can be used at least four times. Simultaneous measurement of another metabolite of lignocaine, glycinexylidide (GX), can be achieved by adjustment of the mobile phase and flow rate. The chromatogram is monitored with an UV detector at 210 nm. The inter- and intra-assay coefficients of variation for MEGX (10-250 micrograms/L) and lignocaine (100-2,000 micrograms/L) are less than 9.5 and less than 2%, respectively, with recoveries for MEGX, trimethoprim (internal standard), and lignocaine all greater than 85%. This method offers a rapid, sensitive assay that is clinically useful in the new role for lignocaine/MEGX in dynamic liver function testing.     

大肠癌旁粘膜细胞的PCNA和AgNORs表达观察

用增殖细胞核抗原（ＰＣＮＡ）和核仁组成区嗜银蛋白（ＡｇＮＯＲｓ）对３５例大肠癌旁粘膜进行细胞增殖表达与观察。结果表明：癌旁粘膜的ＰＣＮＡ标记指数增多，标记增强，排列紊乱。ＡｇＮＯＲｓ在癌旁粘膜中，颗粒增多、增大，形态多样，０￣１ｃｍ和２￣３ｃｍ组的标记指数与４￣５ｃｍ组的标记指数相比较，有显著或非常显著差异（Ｐ〈０．０５或Ｐ〈０．０１）。在ＤｕｋｅＡ、Ｂ期中，细胞标记指数增高增强，与癌的发生和患者     

烧伤患者吸入性损伤和肺部感染的发生特点及其对死亡的影响

为了评价吸入性损伤和肺部感染的发生特点及其对死亡的影响，总结了我科近１４年住院治疗的热力烧伤患者９４０例，其中吸入性损伤７５例，轻度１５例，全部治愈，中度２５例，死亡１３例，死亡率为５２．０％，重度３５例，死亡３１例，死亡率为８８．６％。统计分析表明，合并吸入性损伤者６９．３％在密闭空间发生，同时伴有面部烧伤者达９６．０％。随着烧伤面积的增加，吸入性损伤发生率和肺部感染的发生率相应增加。有吸入性损伤肺部感染较无吸入性损伤肺部感染率为高（Ｐ＜０．０１），发生时间早。两组同等烧伤面积、深度、年龄患者，有吸入性损伤组发生死亡的危险比无吸入性损伤组大１７．２倍（Ｐ＜０．００１）。烧伤面积、深度和年龄相近，合并肺部感染者明显增加了死亡的机会（Ｐ＜０．００１）。     

对中国药典附子中乌头硷限量检查的商榷

用高效液相色谱法测定生附子。炮制附片中的乌头硷、中乌头硷和次乌头硷含量，以及３种生物硷的水解速度，发现炮制附片中主要含次乌头硷，而药典只限定乌头硷含量，显得不够完善。故在此基础上探讨了药典中增加次乌头硷含量限定内容的可能性。     

Electron microscopical demonstration of horseradish peroxidase by use of tetramethylbenzidine as chromogen and sodium tungstate as stabilizer (TMB-ST method): a tracing method with high sensitivity and well preserved ultrastructural tissue.

Until now methods using tetramethylbenzidine (TMB) for electron microscopy (TMB-EM methods) are all unable to provide a maximum demonstration of transported horseradish peroxidase (HRP) while maintaining good ultrastructural tissue preservation. In order to solve this problem, we have attempted to adapt a newly developed, highly sensitive TMB method using sodium tungstate (ST) as the stabilizer (TMB-ST method) for HRP electron microscopic retrograde and anterograde fiber tracing. The present study shows that the TMB-ST method combined with diaminobenzidine-cobalt (DAB-Co) is more sensitive than existing TMB-EM methods and that ultrastructural details are well preserved with this combined method. The resultant reaction product complex after osmication is stable and is observed as characteristic crystal-like structures which are extremely electron dense and often aggregated into clumps. In contrast, the TMB-ST method without the DAB-Co step frequently produces a moderate electron-dense reaction product. Therefore, we recommend the TMB-ST method combined with DAB-Co for HRP electron microscopy.     

用速率法测定尿NAG活性及其临床应用

本文报道用５５０型全自动生化分析仪，以速率法测定尿ＮＡＧ活性及本室尿ＮＡＧ活性的正常参考范围；并对可能影响尿ＮＡＧ活性的尿ＰＨ，尿素浓度等进行了干扰试验及分析。     

江西麦饭石对某些实验动物生理功能影响

用江西麦饭石水浸液喂养小鼠116只,自来水喂养39只,食物条件完全相同,一个月后,发现饮用江西麦饭石水浸液的小鼠体重增长和抗疲劳及耐缺氧能力均优于对照组。用麦饭石任氏液灌注离体蛙心后,心肌收缩力有明显增强。     

金黄色葡萄球菌细胞结合青霉素酶的研究

本文研究了26株P_c~r或O_x~r金黄色葡萄球菌的细胞结合青霉素酶。证实它们除有细胞外青霉素酶还具有细胞结合青霉素酶。金黄色葡萄球菌的总青霉素酶活力为22947～387743u/ml。细胞外青霉素酶活力为140～2130u/ml,占总酶含量的1%以下(菌株85051例外);大量青霉素酶是细胞结合的青霉素酶,活力为19937～382033u/ml。     

羰基二咪唑酰化剂用于头孢菌素的合成

头孢菌素合成常采用混合酸酐法、DCC法、活化酯法等肽类合成方法。羰基二咪唑(简称CDI,I)法也属肽类合成方法。最近有文献报道,将此法用于头孢菌素C_3·位乙酰氧基的改造以及氨苄青霉素α-氨基的修饰。但用于头孢菌素C_7位缩合未见报道。本文采用