Emerging trends in sacubitril/valsartan research: A bibliometric analysis of the years 1995–2021

Background:Sacubitril/valsartan has been approved for the treatment of heart failure (HF) patients with reduced ejection fraction; since then, it gradually became a new star drug in the therapy of HF. Nevertheless, the effectiveness of sacubitril/valsartan remains under investigation. Thus far, only a few bibliometric studies have systematically analyzed the application of sacubitril/valsartan.Methods:Publications on sacubitril/valsartan were retrieved from the Web of Science Core Collection on April 29, 2021. Data were analyzed using Microsoft Excel 2019 (Redmond, WA), VOS viewer (Redmond, WA), and Cite Space V (Drexel University, Philadelphia, PA).Results:A total of 1309 publications on sacubitril/valsartan published from 1995 to 2021 were retrieved. The number of publications regarding sacubitril/valsartan increased sharply in the last 6 years (2015–2021), and American scholars authored >40% of those publications. Most were published in the European Journal of Heart Failure, the United States was the bellwether with a solid academic reputation in this area. Solomon published the highest number of related articles and was the most frequently cited author. “Heart failure” was the leading research hotspot. The keywords, “inflammation,” “fibrosis,” and “oxidative stress” appeared most recently as research fronts.Conclusions:Research attention should be focused on clinical trial outcomes. Considering its effectiveness in HF, the mechanisms and further applications of sacubitril/valsartan may become research hotspots in the future and should be closely examined.     

2种海藻溴酚化合物的抗肿瘤作用及其机制研究

目的:探讨2种海藻溴酚化合物对3株肿瘤细胞Hela,MGC,BGC-823的体外抗肿瘤作用及其作用机制.方法:采用MTT比色法检测不同浓度溴酚化合物对肿瘤细胞的抑制作用;用流式细胞术分析2种化合物对3种肿瘤细胞的细胞周期和非整倍体的诱导作用.结果:2种海藻溴酚化合物对肿瘤细胞均有较强的抑制作用,其中对Hela细胞的抑制活性最强.2种海藻溴酚化合物均不能引起细胞凋亡,但能使肿瘤细胞的倍性改变,产生非整倍体,并使细胞周期受到影响.对Hela细胞和MGC细胞,低浓度能引起亚倍性的产生,较高浓度则引起G1期抑制.对BCC-823细胞,不同浓度处理均能引起G1期抑制,其抑制程度具有剂量效应.结论:海藻溴酚化合物对肿瘤细胞具有不同的生长抑制活性,其作用机制与诱导产生非整倍体和细胞周期抑制有关.     

Optimization of Supercritical Fluid Extraction and Rapid Resolution LC-MS/ESI Identification of Chromones from Saposhnikoviae Radix through Orthogonal Array Design

Objective To establish a rapid and effective supercritical fluid extraction(SFE) and rapid resolution liquid chromatography method coupled with diode-array detector(RRLC-DAD) to quantify the chromones in a species. Methods The effects of four parameters including ethanol concentration(50%-90%), pressure(25-45 MPa), temperature(40-60 ℃), and time(30-90 min) on the chromones yields, namely prim-O-glucosylcimifugin, cimifugin, 5-O-methylvisammioside, and sec-O-glucosylhamaudol, were investigated using SFE system with orthogonal array design(OAD). Furthermore, the extracts were analyzed using rapid resolution liquid chromatography coupled with diode-array detector(RRLC-DAD) system to confirm the results. Results Under the optimized conditions, i.e., 35 MPa of pressure, 60 ℃ of temperature, 70% ethanol, and 60 min of time, the yields of prim-O-glucosylcimifugin, cimifugin, 5-O-methylvisammioside, sec-O-glucosylhamaudol, and total chromones were 3.514, 0.132, 6.242, 0.342, and 10.231 mg/g, respectively. In comparison with ultrasonic assisted extraction(UAE), SFE was able to yield a 20.7% increase in the total chromones from Saposhnikoviae Radix. Conclusion SFE is an alternative and promising method to extract chromones from this species, and the established RRLC-DAD method could serve as a rapid and effective method for the identification of chromones from Saposhnikoviae Radix.     

用于颅颌面种植外科的三维立体可视化系统

目的论文对在个人PC机上对颅颌面医学图像的可视化进行研究,开发出用于颅颌面种植外科的CT断层图像三维可视化系统。方法应用3D纹理映射的方法对颅颌面CT断层图像进行三维重建,并得到其XYZ方向上的切面图像。然后利用边缘检测的方法得到眼眶的位置,并在眼眶位置进行扇形切割,得到18幅扇形切割图像。结果该系统可以帮助医生进行手术前的骨质、骨量分析,设计种植手术的过程及模拟、预测手术的种植效果。结论建立的颅颌面种植外科三维可视化系统以其硬件配置合理、软件设计新颖、多维视角、便捷快速精确等为特色。具有较强的应用价值。     

内皮素-1及其受体在子宫内膜异位症中的表达及意义

目的 研究内皮素-1(ET-1)及其受体(ETAR、ETBR)在子宫内膜异位症(EMs)患者在位子宫内膜、异位子宫内膜以及对照组妇女子宫内膜中的表达,并探讨其在EMs发病机制中的作用.方法 采用免疫组织化学(SP)法检测48例EMs患者(EMs组)在位内膜和异位内膜以及30例宫颈上皮内瘤变患者(对照组)在位内膜中ET-1、ETAR、ETBR的表达.结果 ET-1、ETAR在EMs组在位内膜和异位内膜中的表达均高于对照组(P均＜0.01);ET-1、ETAR在EMs组在位内膜中的表达均高于异位内膜(P均＜0.01);ET-1和ETAR在EMs组在位内膜和异位内膜中的表达均呈显著正相关(相关系数分别为0.970、0.968,P均＜0.05).ETBR在EMs组在位内膜、异位内膜以及对照组内膜三者间的表达差异不具统计学意义.在子宫内膜的不同的组织学周期,ET-1、ETAR、ETBR表达差异均不具统计学意义.在EMs组不同临床期别(r-AFS分期)内膜,ET-1、ETAR、ETBR表达差异均不具统计学意义.结论 ET-1和ETAR在EMs发生发展中具有一定作用.     

中医辨证分期治疗视网膜静脉阻塞86例

目的:观察应用中医辨证分期治疗视网膜静脉阻塞的临床疗效.方法:对86例视网膜静脉阻塞患者初期应用生蒲黄汤加减、中期应用通窍活血汤加减、后期应用驻景丸加减治疗.结果:显效32例,有效40例,无效14例.结论:中医辨证分期治疗可以有效的治疗视网膜静脉阻塞.     

心房颤动患者心房肌组织AT1-R、AT2-RmRNA的表达及研究

目的:分析血管紧张素Ⅱ受体1型(AT1-R)和受体2型(AT2-R)mRNA在心房颤动心房肌组织中的表达情况,并探讨其与心房结构重构的关系.方法:38例因心脏疾患住院接受开胸手术的患者,男18例,女20例,平均年龄(51.97±13.87)岁(18～77岁),分为3组:窦性心律组(SR组,11例),阵发性心房颤动组(pAF组,心房颤动持续时间小于6个月,13例),慢性心房颤动组(cAF组,心房颤动持续时间大于6个月,14例).半定量聚合酶链式反应技术测定AT1-RmRNA、AT2-RmRNA和免疫组织化学技术测定AT1-R受体蛋白在心房肌组织中的表达情况.结果:与SR组和pAF组相比,cAF组患者的平均左心房直径明显增大(P＜0.01),左心室射血分数降低(P＜0.05);心房肌组织中AT1-RmRNA表达明显下调(P＜0.01～0.05),AT2-R mRNA表达明显上调(P＜0.05).AT1-R mRNA表达与左心房直径负相关(P＜0.005);AT2-R mRNA表达与左心房直径正相关,与左心室射血分数负相关.AT1-R蛋白阳性染色在SR组主要见于细胞膜,在cAF组患者主要见于成纤维细胞和血管平滑肌细胞.结论:cAF时,AT1-RmRNA表达下调,AT2-R mR-NA表达上调,说明存在组织肾素-血管紧张素系统(RAS)激活,RAS可能是通过促进心房结构重构形成,从而影响心房颤动的发生.     

成人脊髓圆锥水平的脊髓和硬膜囊径线MRI研究

目的 :评价正常成人脊髓圆锥水平脊髓和硬膜囊矢径、横径的正常值范围及在临床实践中的应用价值。方法 :对 1 5 6例正常成人行脊髓圆锥最大矢径横截面水平T1 WI横断面扫描 ,测量该处脊髓与硬膜囊的矢径、横径 ,计算其平均值、标准差及矢径与横径比、近似面积。结果 :脊髓圆锥最大矢径横截面多位于T1 2 椎体 (82例 ,5 2 5 7% ) ,脊髓矢径 (6 86± 0 75 )mm ,横径 (8 4 1± 0 88)mm ,矢横比 0 75～ 0 85 ,硬膜囊矢径 (1 3 2 6± 1 6 1 )mm ,横径 (1 7 5 8± 2 6 8)mm ,矢横比 0 6 5～ 0 80 ,各径线大小无性别、年龄和身高差异 ,脊髓与硬膜囊矢径成正相关。结论 :熟悉脊髓和硬膜囊径线的正常值范围对临床诊断髓内肿瘤与硬膜囊等病变有较大帮助