Subclinical Anterior Horn Cell Involvement in Juvenile Myoclonic Epilepsy

Summary: Although clinical signs of muscle wasting and weakness were not present, electromyographic (EMG) evidence of subclinical anterior horn cell involvement of spinal cord was noted in 5 patients with juvenile myoclonic epilepsy (JME). Quantitative interference pattern analysis of EMG recorded from the anterior tibial muscle showed that the ratio (amplitude:turn/turn:second, A:T/T:S) was significantly increased in 10 patients with JME and 12 patients with lower motor neuron disorders (LMND) as compared with those of 22 normal subjects and 15 patients with frequent generalized tonic-clonic seizures (GTC). Subclinical anterior horn cell involvement detected by EMG techniques can be related to a genetically determined component of JME.     

Effects of gonadotrophin treatment in vivo on testicular function in immature rhesus monkeys (Macaca mulatta)

Changes in testicular histology and concentrations of testosterone and oestradiol 17β in testicular tissue and plasma have been studied following administration of gonadotrophins (oFSH, oLH, hCG and PMSG) to immature male monkeys. Treatment with FSH (1 mg/day) or PMSG (100 IU/day) for five days, induced a marked enlargement of the seminiferous tubules and increase in the Sertoli cell cytoplasm. Injections of LH (1 mg/daily) or hCG (100 IU/daily) administered similarly, failed to produce hypertrophy of the Sertoli cell. In LH, hCG and PMSG stimulated testes morphologically differentiated interstitial cells could be recognized. FSH did not produce any detectable effect on the intertubular tissue. A significant increase in testicular and plasma testosterone levels was observed with LH, hCG and PMSG. FSH was shown to be much less effective in stimulating androgenesis. An increase in testicular oestradiol production over that of controls, was observed in FSH and PMSG treated monkeys but not in animals treated with LH or hCG.     

Comparison of thickness measurements in cutaneous and subcutaneous lesions preoperatively (by ultrasonography) and postoperatively (by ruler)

Vertical thickness of cutaneous and subcutaneous lesions cannot be assessed accurately by simple examination. To achieve this ultrasonography was performed preoperatively and ruler assessment postoperatively. These measurements were then compared and evaluated using the Wilcoxon test. The results were not statistically significant (p=0.6592, z=0.4410). Cutaneous and subcutaneous ultrasonography is a simple, quick, accurate method of assessing lesion thickness. Received: 29 April 1997 / Accepted: 7 July 1997     

Effect of temperature and pregnant mare's serum gonadotropin on testicular function in the spiny-tailed lizard,Uromastix hardwicki

The effect of temperature and pregnant mare's serum gonadotropin (PMSG) was observed on the testes of Uromastix hardwicki at a time of the year (November) when the seminiferous epithelium contained abundant proliferating spermatogonia and some primary spermatocytes. This recrudescent state of the testes was maintained by keeping the animals at 20° for 10 days. But, in lizards kept at 35°, there was a marked decrease in the testis weight. The seminiferous tubules were lined mostly with Sertoli cells and a few resting types of spermatogonia. The interstitial cells were only slightly affected and there was no reduction in androgen concentrations in the testicular tissue or plasma, as measured by testosterone radioimmunoassay. At 20°, injections of 20 IU of PMSG daily for 10 days induced an increase in the number of primary spermatocytes and production of spermatids in certain regions of the tubules. In the PMSG-treated lizards kept at 35° the testes contained a single layer of resting spermatogonia placed along the basement membrane. Under these conditions PMSG failed to maintain the active state of the seminiferous epithelium. Unlike the germinal cells, the interstitial cells were markedly stimulated by PMSG at 20 or 35°. Testicular and plasma androgen levels were increased severalfold in gonadotropin-treated lizards. The elevation of androgen levels as a result of PMSG stimulation was higher in lizards kept at 35°. It is suggested that in Uromastix hardwicki, the germinal epithelium responds differentially to PMSG treatment at the two different temperatures and that the activity of the interstitial cells is not inhibited by hyperthermia.     

Synthesis of 1,2,5(6)-Trisubstituted Benzimidazoles and Evaluation of Their Antimicrobial Activities

A series of 22 benzimidazoles, having several substituents on the azole and benzene nuclei, were prepared and evaluated in vitro for antimicrobial activity. At first 2-chloro or 2-chloromethyl-5(6)-substituted-1H-benzimidazoles were synthesized, which were then substituted at C-2 with several piperazine or piperidine derivatives. The antibacterial activity of these compounds against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Pseudomonas aeruginosa, and the antifungal activity against Candida albicans, Candida stellatoidea, Candida parapsilosis, and Candida pseudotropicalis were determined as the MIC values. Since compound 12 exhibits good activity, in order to clarify the effect of substituents at C-1 on the activity, benzimidazole derivatives having ethyl, allyl, benzyl, and p-fluorobenzyl substituents at C-1 were prepared, and slightly increased activity was seen.     

Extra-skeletal Ewing's sarcoma of the submandibular gland.

Extra-skeletal Ewing's sarcoma (EES) is an uncommon malignancy, especially in the head and neck region that may arise in various extra-osseous tissues. We report a 22-year-old male with an EES of the submandibular gland, which to the best of our knowledge, has not been described previously. The patient who underwent combined treatment with surgical resection and chemo-irradiation was disease free for 22 months but succumbed to multi-organ metastases 14 months later. This case highlights the combined diagnostic role of immunohistochemical, cytogenetic and radiological evaluation of EES. EES is an aggressive cancer that requires multidisciplinary management with wide surgical excision and adjunctive chemo-irradiation for the best outcome.     

Assessment of bone density differences between conventional and bone-condensing techniques using dual energy x-ray absorptiometry and radiography.

OBJECTIVES: We used dual energy x-ray absorptiometry (DEXA) and periapical radiography to assess bone density differences after conventional and bone-condensing dental implant techniques. STUDY DESIGN: Single-tooth dental implants were placed by both conventional and bone-condensing techniques in 14 patients with bilateral missing teeth. Dual energy x-ray absorptiometry was used to calculate bone mineral density (BMD) and bone mineral content (BMC) before and 6 and 12 months after implant placement. Furthermore, photodensitometry of periapical radiographs was also assessed. RESULTS: The success rate was 92.9% for the conventional technique and 71.5% for the bone-condensing technique. The BMD was observed to be significantly higher 6 and 12 months after implant placement. The BMC and photodensitometry were significantly increased 6 months after implant placement but showed no further after 12 months. CONCLUSIONS: There were no significant differences in BMD, BMC, and photodensitometry between the two implant placement techniques. However, success rate of the conventional technique was greater than the bone-condensing technique, which may be the result of trabecular fracture associated with the bone-condensing.