吊顶灯辅助下25G微创玻璃体切割手术治疗眼弓蛔虫病的疗效

目的：探讨吊顶灯辅助下25G微创玻璃体切割手术治疗眼弓蛔虫病（OT）的疗效，并分析术后并发 症。方法：回顾性系列病例研究。选择2014年12月至2019年2月在徐州市立医院眼科确诊为OT的 患者27例（27眼）。所有患者经过1～2个月的全身或局部皮质类固醇治疗后接受了25G微创玻璃体 切割手术，避开周边病灶区放置灌注管和吊顶灯，切除玻璃体及牵拉条索，根据术中视网膜情况予 激光光凝、气液交换、玻璃体腔填充空气或C3F8或硅油，部分联合晶状体摘除、环扎术。分析患者 的临床特征、光学相干断层扫描（OCT）、眼底照相检查、治疗情况和术后并发症等，计算术后随访 期内葡萄膜炎复发率和一次性视网膜解剖复位率。对手术前与末次随访最佳矫正视力（BCVA）进行 t检验分析。结果：27例患者中周边部肉芽肿型11眼，后极部肉芽肿型11眼，眼内炎型5眼。伴视网 膜前膜（ERM）23眼；伴牵拉性视网膜脱离（TRD）13眼，其中TRD合并ERM 11眼，单纯TRD 2眼。 术后随访6～44（17.6±11.0）个月。术后早期低眼压4眼（15%），高眼压1眼（4%）。伴TRD的13眼中 11眼经一次手术即实现解剖复位，一次性视网膜复位率为85%；伴ERM的23眼中术后复发2眼（9%）； 术后视网膜脱离2眼（7%），术后并发白内障3眼（11%）。27眼中有4眼（15%）于术后1～5个月炎症复发， 其中1眼经再次手术后炎症消退，另外3眼予全身及局部皮质类固醇治疗2个月内炎症得到控制。术 前及术后末次随访BCVA（logMAR）分别为1.46±0.66、1.13±0.66，术后视力较术前明显提高，差 异有统计学意义（t=4.009，P<0.001）。结论：吊顶灯辅助下25G微创玻璃体切割手术治疗眼弓蛔虫病 可有效控制葡萄膜炎症，获得较满意的疗效。     

修复前关节囊在"肘关节恐怖三联征"手术治疗中的疗效观察

目的探讨修复前关节囊在"肘关节恐怖三联征"手术治疗中的疗效。 方法自2015年5月至2017年12月苏州大学附属瑞华医院手外科采用手术修复前关节囊治疗8例肘关节恐怖三联征患者，根据影像学评价观察骨折愈合情况。采用Mayo肘关节功能评分评估肘关节功能情况。 结果所有患者均获得6~36个月随访，平均18个月。切口均Ⅰ期愈合。骨折均愈合，时间为8~12周，平均10周。根据术后6个月随访，肘关节屈位0°~15°，伸位130°~145°，平均活动范围为115°，旋前60°~90°，旋后40°~70°，平均旋转范围120°。术后无骨折块移位、内固定失效、锁定接骨板螺钉松动或断裂、切口感染、异位骨化等并发症发生。肘关节功能恢复良好，采用Mayo肘关节功能评分：优6例，良2例。 结论在肘关节恐怖三联征时修复前关节囊，恢复肘关节的稳定性，并发症少，骨折愈合快，及早配合正规的康复锻炼，肘关节功能恢复好，疗效确切。     

Effect of C60 on the phase transition behavior of a lipid bilayer under high pressure

Interactions between fullerenes and cells and effects on the main transition of lipid bilayers have attracted much attention in biophysics in recent years. By employing coarse-grained molecular dynamics simulations, we obtained the temperature–pressure phase diagrams of a dipalmitoylphosphatidylcholine bilayer, which exhibits a gel phase and a fluid phase, with variation of the C₆₀versus lipid ratios. The simulation results show that the critical area per lipid at the fluid–gel main phase transition boundary increases with the increasing ratios of C₆₀. A critical area per lipid of 0.594 ± 0.002 nm² is obtained when the ratio of C₆₀ reaches 6.4% while that of the pure bilayer case is 0.572 ± 0.002 nm². The main transition temperature, T_m, remains almost unchanged with the addition of C₆₀ below a ratio of 4.7%, while a 2 K decrease of T_m is observed at a ratio of 6.4% under various pressures. Consequently, the presence of C₆₀ in the bilayer, with the ratio of C₆₀ less than 4.7%, will not influence the main transition behavior of the bilayer even under pressure as high as 1500 bar. The radial distribution function analyses suggest that the presence of C₆₀ produces no impact on the radial distribution of the lipids in the bilayers. The lateral density profiles show that the incorporation of C₆₀ with relatively high ratios stabilizes the thickness of the bilayer.

By employing coarse-grained molecular dynamics simulations, we obtained the temperature–pressure phase diagrams of a dipalmitoylphosphatidylcholine bilayer, which exhibits a gel phase and a fluid phase, with variation of the C₆₀versus lipid ratios.      

In vivo detection of salicylic acid in sunflower seedlings under salt stress

Salicylic acid (SA) is an important phytohormone. It plays an essential role in regulating many physiological processes of plants. Most of the conventional methods for SA detection are based on in vitro processes. More attention should be paid to develop in vivo methods for SA detection. In this work, Pt nanoflowers and GO were simultaneously electrodeposited and reduced on a Pt wire microelectrode in one step. The Pt nanoflowers/ERGO modified Pt microsensor demonstrated high sensitivity and selectivity for SA. SA could be detected from 100 pM to 1 μM with a detection limit of 48.11 pM. Then this microsensor was used to detect SA in the stem of sunflower seedlings under different salt stresses in vivo. The result showed that with the increasing concentration of salt, SA levels decreased. Our result was also confirmed by UPLC-MS and gene expression analysis. To the best of our knowledge, this is the first report of in vivo detection of SA in plants using the Pt nanoflowers/ERGO modified Pt microelectrode. It is foreseeable that our strategy could pave the way for the in vivo detection of phytohormones in plants.

A Pt nanoflowers/ERGO modified Pt microelectrode was proposed to detect salicylic acid in plants under salt stress in vivo.     

前列腺癌CK5^+CK8^+细胞的分选及其分子生物学特性

目的在人前列腺癌细胞系中分选CK5^+CK8^+细胞,了解其分子生物学特性。方法流式细胞术在人前列腺癌细胞系PC3和LNCaP中分选CK5^+CK8^+细胞,用实时荧光定量聚合酶链反应(RT-PCR)和蛋白质印迹法方法检测LNCaP细胞分选的CK5^+CK8^+细胞CK5、CK8、雄激素受体(AR)和前列腺特异抗原(PSA)的表达,细胞迁移实验检测CK5^+CK8^+细胞迁移能力,琼脂糖凝胶克隆形成实验检测CK5^+CK8^+细胞成瘤能力。采用t检验进行统计学分析。结果采用流式细胞术可以在PC3中分选出(21.3±4.6)%的CK5^+CK8^+细胞,在LNCaP中分选出(1.2±0.4)%的CK5^+CK8^+细胞。与非CK5^+CK8^+细胞相比,LNCaP中分选的CK5^+CK8^+细胞CK5 mRNA表达差异有统计学意义(t=10.435,P<0.001),CK8 mRNA表达差异无统计学意义(t=1.974,P=0.121),AR和PSA mRNA表达差异有统计学意义(t=4.317,3.232;P=0.016,0.037)。蛋白质印迹法检测得到类似结果。细胞培养7 d后,CK5^+CK8^+细胞和非CK5^+CK8^+细胞均可以在Transwell小室迁移生长,迁移细胞数分别为(60±7)个和(32±5)个,2者比较差异有统计学意义(t=6.031,P=0.004)。细胞培养14 d后,CK5^+CK8^+细胞和非CK5^+CK8^+细胞均可以在软琼脂糖凝胶中克隆性生长,阳性克隆数分别为(71±5)个和(27±3)个,差异有统计学意义(t=13.009,P<0.001)。结论人前列腺癌细胞系LNCaP和PC3都可以分选出CK5^+CK8^+细胞,LNCaP中CK5^+CK8^+细胞AR和PSA均有一定程度表达,迁移和成瘤能力增强。     

Reversing multidrug resistance by intracellular delivery of Pluronic P85 unimers

Pluronics have been demonstrated as excellent multidrug resistance (MDR) reversal agent in the form of unimers rather than micelles. However, the effective intracellular delivery of Pluronic^® unimers to MDR cancer cells still remains a big challenge. To address this issue, a mixed micellar system based mainly on the pH-sensitive copolymer of poly (l-histidine)-poly (d,l-lactide)-polyethyleneglycol-poly (d,l-lactide)-poly (l-histidine) (PHis-PLA-PEG-PLA-PHis) and Pluronic^® F127, some of which was conjugated with folate, was constructed to intracellularly deliver the unimers of Pluronic^® P85 to MDR cells. The folate-mediated endosomal pH-sensitive mixed micelles (pH_endoSM-P85/f) were prepared by a thin-film hydration method, by which Pluronic^® P85 unimers and doxorubicin (DOX) were incoporated into the mixed micelles. The incorporation of Pluronic^® P85 unimers was investigated by the surface tension test. The results indicated that the Pluronic^® P85 unimers probably first inserted into the binary mixed micelles and then formed a triple-component mixed micelles with Pluronic^® F127 and PHis-PLA-PEG-PLA-PHis as the loading content increased. Further analyzed with flow cytometry, confocal laser scanning microscopy (CLSM) and MTT assay, the micelles with inserted Pluronic^® P85 unimers demonstrated much more cellular uptake and higher cytotoxicity against MDR cells than the triple-component mixed micelles and plain Pluronic^® micelles. The enhanced MDR reversal effect was attributed to the successful intracellular delivery of Pluronic^® P85 unimers to the MDR cells, which was confirmed by the subcellular colocalization of Pluronic^® P85 unimers with mitochondria, the decreased ATP energy and mitochondrial membrane potential (MP) in the MCF-7/ADR cells. The pH_endoSM-P85/f/DOX also demonstrated more dramatic antitumor efficiency and remarkable reduction of ATP energy in the MDR cells in tumors than the control formulations. The intracellular delivery of Pluronic^® P85 unimers to the MDR cells based on the targeted and endosomal pH triggerd release mixed micelles has been demonstrated as a promising approach to reverse MDR.