rhGM-CSF作为成人乙肝疫苗无(弱)应答者免疫佐剂初探

Objective To investigate the effect of recombinant human granulocyte macrophage colony stimulating factor (rhGM-CSF) as adjuvant on immune response in adults of non-and hyporesponders to hepatitis B vaccine. Methods Those who were once immunized with recombined yeast gene hepatitis B vaccine more than one standard scheme in two years and negative for hepatitis B markers were randomly sorted as group A and group B. 33 adults of group A were given hepatitis B vaccine 10 μg each time. The immune procedure was O, 1 and 6month. 34 adults of group B were given rhGM-CSF 300 μg for the first day, then 10 μg each time for routine immune. The blood samples were collected before the first injection and in 1, 2 and 8 months (T1, T2, TS)following the first injection to test Anti-HBs. Results Anti-HBs positive conversion rates of group A and B at T8 was 39.39% and 64.71% respectively(P=0.038). Anti-HBs levels of group B at TI, T2, T8 were(113.85±198.56) mIU/ml, (312.40±349.44) mIU/ml, (427.74±411. 58) mIU/ml (P=0.001). There was significant difference between group A and B in T8 Anti-HBs levels(P=0.010). Conclusion Better immune response was found in the group of rhGM-CSF with hepatitis B vaccine. So rhGM-CSF can induce the immune respond to hepatitis B vaccine.     

重组人bFGF的原核表达及其高效价抗血清的制备

目的 以重组人碱性成纤维生长因子为免疫原，制备高效价抗hbFGF抗血清。方法通过PCR方法改造5’编码区的12个密码子，构建hbFGF’原核表达载体并在大肠杆菌(E．coli)中表达，以纯化的hbFGF、免疫新西兰兔，制备高效价抗血清，用于重组hbFGF、的免疫印迹分析。结果经过改造的hbFGF基因在E．coli中获得较高水平表达。从可溶性部分纯化得到纯度95％以上的重组hbFGF，以该重组蛋白免疫兔子，在二次加强后以间接ELISA检测抗血清效价可达1：512000。免疫印迹分析显示该抗血清与E．coli中表达的重组hbFGF、和标准hbFGF、均有特异性反应，但与某些细菌蛋白存在弱交叉反应，经E．coli菌体蛋白吸附的抗血清，与菌体蛋白的弱交叉反应消失。结论以纯化的重组hbFGF为免疫原制备了高效价的特异性抗血清，经菌体蛋白吸附可消除存在的交叉反应性。     

中晚期重型乙型肝炎血浆置换与内科治疗生存率的比较

观察血浆置换、内科治疗对中晚期重型乙型病毒性肝炎生存率的影响,探索中晚期重型肝炎的治疗方法。方法观察在内科治疗基础上给予血浆置换患者的肝功能、并发症及疾病的转归,与同期仅予内科综合治疗的患者相比较,对相应的临床资料进行统计学分析,从而了解两种治疗方法的疗效。结果血浆置换组36例,18例好转、18例死亡,内科治疗组32例,13例好转、19例死亡,两组之间患者生存情况无统计学差异;肝功能指标(ALT、AST、SB、ALB、TC、ChE和凝血酶原时间(PT)也无统计学差异。结论与内科治疗相比较,在其基础上给予血浆置换并不能提高中晚期重型病毒性肝炎生存率;中晚期重型肝炎患者的预后决定于其肝功能衰竭的程度。     

Initial evaluation of a continuous speech recognition program for radiology

The aims of this work were to measure the accuracy of one continuous speech recognition product and dependence on the speaker's gender and status as a native or nonnative English speaker, and evaluate the product's potential for routine use in transcribing radiology reports. IBM MedSpeak/Radiology software, version 1.1 was evaluated by 6 speakers. Two were nonnative English speakers, and 3 were men. Each speaker dictated a set of 12 reports. The reports included neurologic and body imaging examinations performed with 6 different modalities. The dictated and original report texts were compared, and error rates for overall, significant, and subtle significant errors were computed. Error rate dependence on modality, native English speaker status, and gender were evaluated by performing ttests. The overall error rate was 10.3 +/- 3.3%. No difference in accuracy between men and women was found; however, significant differences were seen for overall and significant errors when comparing native and nonnative English speakers (P = .009 and P = .008, respectively). The speech recognition software is approximately 90% accurate, and while practical implementation issues (rather than accuracy) currently limit routine use of this product throughout a radiology practice, application in niche areas such as the emergency room currently is being pursued. This methodology provides a convenient way to compare the initial accuracy of different speech recognition products, and changes in accuracy over time, in a detailed and sensitive manner.     

癫癎患者脑血流速度改变与癫癎灶定侧的关系

目的 :探讨癫发作间期脑血流变化对癫灶定侧的价值。方法 :4 7例癫病人发作间期行经颅多普勒超声脑血流速度测定 ,并与临床、脑电图定位和MRI/CT病灶对比分析。结果 :根据临床症状确定病灶侧的 19例病人中 ,病灶侧脑血流速度改变 (升高和降低 )的比率显著高于双侧脑血流速度对称者 ;在脑电图确定病灶侧的 2 4例病人中 ,病灶侧脑血流速度升高比率显著高于脑血流速度降低和双侧脑血流速度对称者 ;在MRI确定病灶侧的 13例病人中 ,病灶侧脑血流速度增快 7例 ,减慢 2例 ,双侧血流速度对称 4例。结论 :癫病人一侧脑血流速度增高 ,高度提示癫病灶侧。     

PCOS患者AMH水平与性激素及胰岛素抵抗的相关性研究

目的 探讨多囊卵巢综合征(PCOS)患者血清抗苗勒管激素(AMH)水平与性激素及胰岛素抵抗(IR)的相关性.方法 选取2019年1月至12月在西安交通大学第一附属医院妇科内分泌门诊及西北工业大学医院妇科确诊的PCOS患者109例,测定患者血清AM H、性激素水平,应用稳态模型评估胰岛素抵抗指数(HOMA-IR),分析PCOS患者不同AM H水平的性激素及IR值的差异,以及性激素、IR对AM H的影响.结果 对PCOS患者,根据血清AM H检测值按百分位(<25％,25％ ～75％,>75％)进行分层,随着AMH百分位逐渐升高,体质量指数(BMI)、HOMA-IR值均降低(F值分别为3.354、3.712,P<0.05),同时黄体生成素(LH)、睾酮(T)值均升高(F值分别为3.355、3.317,P<0.05).相关性分析显示,血清AMH水平与BMI、HOMA-IR均呈显著负相关(r分别为-0.228、-0.214,P<0.05),与L H、T均呈显著正相关(r分别为0.344、0.327,P<0.01).进一步多元回归分析显示,在控制BMI后,LH及T对AMH存在显著正向影响(β=0.258,P<0.05;β=0.291,P<0.01).结论 PCOS患者血清AMH水平与LH、T均呈正相关,其与HOMA-IR的负相关关系可以由BMI解释.     

肝移植病人的ICU期观察及护理

目的：通过对8例肝移植、1例肝肾联合移植术后护理经验的总结,介绍肝移植病人的护理重点及方法;方法：隔离室准备、神经系统及生命体征监测、排斥反应监测、预防感染及心理护理;结果：肝移植术后早期（ICU）的护理至关重要,直接关系到近远期疗效和生存率;结论：新技术的开展要求高素质的护理人员和高质量的护理水平。     

周围型肺癌的螺旋CT增强扫描

目的 :评价螺旋CT增强薄层扫描对周围型肺癌的诊断价值。方法 :2 7例周围型肺癌 ,对结节进行薄层扫描 ,然后以 3 5ml/s肘静脉注射碘对比剂 10 0ml后 40s、2min、5min、8min于相同几个中心层面进行增强扫描。分析结节的强化类型 ,描绘结节的时间 -密度曲线 ,计算结节的最大增强CT值。结果 :根据病灶的强化特点 ,我们将周围型肺癌强化类型分为二型 ,即均匀强化型和不均匀强化型。本组 2 7例肺癌 ,其中 17例 (6 3 % )在注射对比剂后 2min达到高峰 ,另外 10例 (37% )在注射对比剂后 5min达到峰值。本组病例中肺癌最大强化CT值 2 2～ 5 5HU(2 5 / 2 7) ,平均为 (4 1 8± 14 5 )HU。结论 :螺旋CT增强薄层扫描能准确判断病灶增强程度及方式 ,对提高周围型肺癌的诊断准确率有较大帮助。     

糖尿病患者膳食营养分析

目的 :探讨糖尿病患者膳食营养状况 ,提出改善糖尿病患者膳食结构的策略。方法 :对 2 2 5例糖尿病患者连续调查 3天膳食供给情况 ,建立数据库输入计算机 ,用SPSS软件进行分析。结果 :热能摄入量不符合要求者占77 3% ;蛋白质供给量不足者占患者总数的 75 6% ;47 6%的糖尿病患者脂肪摄入量超过标准 ;5 5 1%的糖尿病患者碳水化合物的摄入量不符合要求 ;膳食纤维摄入不足者占患者总数的 94 2 % ;维生素和无机盐中 ,除铁、核黄素和抗坏血酸供给量尚可 ,其余均不足。结论 :糖尿病患者膳食是不合理的 ,必须按照膳食治疗方案调节膳食     

软组织肉瘤免疫组织化学研究的意义

本文旨在研究免疫组织化学在软组织肉瘤诊断、鉴别诊断的意义。判断肿瘤增生程度及预防 ,进展及治疗前景。