吸液态氧对高原移居青年氧耗量的影响

目的 :探讨在高原吸入液态氧对移居青年氧耗量的影响。方法 :将进驻海拔 370 0m半年的 4 0名健康青年随机分为两组 ,每组 2 0人。对照组用EGM型踏车功量计做坐位踏车运动 ,初始负荷功率 2 5W ,每 3min递增 5 0W ,以 6 0r/min连续踏车直至力竭。实验组在运动前 10min开始用面罩吸液态氧 ,每分钟吸入量为 4L ,在踏车运动中全程吸氧 ,踏车方式同对照组。结果 :心率在 10 0～ 170 /min时 ,两组氧耗量随心率增加而呈线性增加 (P <0 .0 1)。结论 :在高原运动时吸液态氧能提高移居青年心脏作功效率和改善肺功能。     

多层螺旋CT无创性冠状动脉造影对冠状动脉疾病的诊断价值

目的:探讨16层螺旋CT冠状动脉造影对冠状动脉病变的临床诊断价值。方法:57例疑似冠状动脉病变的患者行16排螺旋CT检查和冠状动脉造影,不包括5例为冠状动脉支架置入术后(支架12个)。将冠状动脉分为13个节段,分析所有管腔大于2mm的节段,评价图像是否能满足管腔评价。管腔狭窄大于50%认为有意义,以常规冠状动脉造影作为金标准,比较16层螺旋CT在诊断有意义的冠状动脉狭窄方面的敏感性、特异性、阳性预测值、阴性预测值。结果:在所有638个节段中,588个节段(占92.16%)能够满足冠状动脉管腔评价。对于16层螺旋CT能够显示有意义冠状动脉狭窄,与ICA相对照,若以动脉节段计数,MSCT显示中度以上狭窄的敏感性、特异性、阳性预测值、阴性预测值和准确度分别为93.61%、91.11%、89.79%、98.12%和94.63%。以动脉主干计数,MSCT显示中度以上狭窄的敏感性、特异性、阳性预测值、阴性预测值和准确度分别为92.82、、91.31%、80.43%、97.37%和82.08%。以患者计数,MSCT显示中度以上狭窄的敏感性、特异性、阳性预测值、阴性预测值和准确度分别为94.73%、90.00%、93.01%、92.99%和82.75%。结论:16层螺旋CT结合回顾性心电门控技术冠状动脉成像无创、简单易行。既能显示管腔情况又能显示冠脉管壁,弥补了ICA的不足;经严格控制影响因素,具有较高的敏感性和特异性,较高的阴性预测值可避免不必要的有创性检查。对支架术后的随访也有较高的应用价值,对血管变异的显示直观确切。可作为一种有效的筛查和随访手段应用于临床。     

人IL-1 Ra基因真核表达载体的构建及体外转染大鼠骨髓间充质干细胞

目的:构建人IL-1Ra真核表达载体,体外转染大鼠骨髓间充质干细胞(BM-MSCs)。方法:从PHA活化的人外周血单个核细胞中提取总RNA,RT-PCR法获得hIL-1Ra cDNA,经HindⅢ、EcoRⅠ双酶切,定向克隆到真核表达载体pcD-NA3。经酶切分析及PCR鉴定后,脂质体介导下体外转染大鼠BM-MSCs,免疫荧光法检测hIL-1Ra在大鼠MSCs的表达。结果:获得570 bp大小的IL-1Ra cDNA片段;经酶切分析、PCR鉴定及DNA测序,证实IL-1Ra cDNA定向插入pcDNA3质粒,目的序列与GenBank源序列完全一致;脂质体介导转染48h后,免疫荧光检测到hIL-1Ra在大鼠MSCs的表达。结论:成功构建pcDNA3-hIL-1Ra真核表达载体并在大鼠BM-MSCs中表达,为进一步的hIL-1Ra基因修饰MSCs研究提供了实验基础。     

Do socioeconomic characteristics of neighbourhood of residence independently influence incidence of coronary heart disease and all-cause mortality in older British men?

BACKGROUND: The relationship between coronary heart disease (CHD) incidence and death, and individual sociodemographic status is well established. Our aim was to examine whether neighbourhood deprivation scores predict CHD and death in older men, independently of individual sociodemographic status. METHODS: Prospective study of 5049 men, born between 1918 and 1939, recruited from 24 British towns encompassing 969 electoral wards, without documented evidence of previous major CHD when responding to a questionnaire in 1992, and followed up for incidence of major CHD and death. RESULTS: Four hundred and seventy-two new major CHD events (1.08% pa), and 1021 deaths (2.28% pa) occurred over an average of 9.75 years. When men were divided into fifths according to increasing neighbourhood deprivation score, CHD incidences (% pa) were 0.92, 0.89, 0.99, 1.33 and 1.29. When modelling continuous trends, the rate ratio for men in the top fifth compared with the bottom fifth was 1.55 (95% confidence interval 1.19-2.00) for CHD. This rate ratio was, however, no longer statistically significant [1.22 (95% confidence interval 0.92-1.61)] when effects of individual sociodemographic status measures (car ownership, housing, longest held occupation, marital status and social networks) were accounted for. CONCLUSION: Little evidence of an independent relationship of neighbourhood deprivation with CHD incidence was found once individual measures of sociodemographic status had been adjusted for.     

中药抗疲Ⅰ号对防治应激性急性肾衰作用的肾基底膜观察

目的用甘油致成应激性急性肾功能衰竭家兔实验模型,观察中药抗疲Ⅰ号的防治效果。方法阳性对照组和阴性对照组大鼠正常喂养,实验组在饲料中加入抗疲劳Ⅰ号喂养,1月后阳性对照组和实验组分别经腹腔注射甘油致成应激性急性肾功能衰竭家兔实验模型,三组继续以上喂养方法。均于造模第1、5和14日测定三组血尿钠、钾和肌酐,肾组织行电镜观察肾滤过膜和吸收膜的损伤与防治变化。结果实验组的肾滤过膜和吸收膜的早期损伤明显比阳性对照组轻,14d时滤过膜和吸收膜的结构已修复。结论抗疲Ⅰ号具有防护和修复肾组织超微结构的功能,各项肾功检测指标表明抗疲Ⅰ号具有防治应激性急性肾衰的作用。     

Hypnotherapy: an effective treatment modality for trichotillomania

This study describes a non-pharmacological treatment modality for children with trichotillomania. Three children with trichotillomania were treated using a hypnotherapy technique. All patients were observed in the outpatient clinic for 8 consecutive weeks and subsequently followed for 12-18 months. All children were cooperative in performing the hypnotherapy technique (relaxation/mental imagery). Two patients reported complete resolution of their complaints after 7-8 weeks and 1 patient after 16 weeks. The latter, reporting recurrence of the complaint after 4 weeks due to stressful school problems, was resolved after successful retreatment over 3 weeks. During a mean follow-up period of 16 months, there were no recurrences. In conclusion, hypnotherapy may be considered as a primary treatment modality for trichotillomania in children without associated emotional disorders.     

用模糊数学方法评价γ辐照装置的环境辐射水平

目的 评价γ辐照装置环境辐射水平以保证放射性工作人员和环境安全。方法 采用模糊数学方法对济宁辐照中心扩建γ辐照装置的环境辐射水平进行综合评价。结果 该装置选址合理，防护和环境保护措施应用得当，对周围环境无明显影响。结论 综合评价达到优级水平。     

不同药物对海拔3700m移居青年心功能指数的影响

目的　研究筛选改善高原移居青年心功能提高做功效率的药物。方法　对海拔 370 0m已习服的 4 0名士兵随机分为 4组 ,每组 1 0人。让受试者做坐位踏车运动。计算每位受试者的心功能指数。然后 4组分别口服酪氨酸、乙酰唑胺、依那普利加硝苯地平、安慰剂 (对照组 ) ,每天 2次 ,连服 1 5d。在服药的第 1 0天和第 1 5天 ,停药后的第 1 0天和第 2 0天时重复上述运动实验。结果　服药的第 1 0天 ,第 1 5天 ,停药后的第 1 0天 ,第 2 0天时 ,三组心功能指数均较服药前及对照组增高 (P <0 .0 5或P <0 .0 1 ) ,酪氨酸组优于其它两组。结论　改善高原移居青年心功能提高做功效率的药物应首选酪氨酸