佐剂诱导关节炎大鼠模型的建立及成纤维样滑膜细胞的分离

目的建立类风湿性关节炎(RA)动物模型；从炎性关节滑膜中分离成纤维样滑膜细胞(FLS)。方法应用热灭活结核杆菌H37Ra菌株与矿物油混合制备改良的佐剂,尾根部皮内注射Lewis大鼠诱导关节炎；剪取成功诱导关节炎大鼠的病变踝关节,从中剥离滑膜组织,充分剪碎后采用胶原酶消化法分离成纤维样滑膜细胞。结果成功诱导了大鼠关节炎,发病率为100%,发病时间有规律,组织学表现与RA相似；成功在体外培养了FLS并对其进行了鉴定,掌握了其生长形态和特征。结论成功制备RA动物模型并获得FLS,为今后RA发病机制探索和药物评价提供了良好的体内动物模型和体外细胞模型。     

From Atretic to Perforated: A Closer View of Pulmonary Atresia

A 7‐day‐old male infant with membranous pulmonary atresia and intact ventricular septum (PA/IVS) underwent hybrid pulmonary valvotomy. Access was via median sternotomy guided by intra‐operative epicardial ultrasonography. After puncturing the atretic pulmonary valve, a 6 mm Wanda balloon catheter was inflated to dilate the atretic valve. Adequate valve movement with antegrade flow was confirmed using real time epicardial echocardiography. Intra‐operative epicardial ultrasonography is an effective tool for guiding PA/IVS during hybrid procedures. Epicardial echo imaging provides clear images with excellent quality, helps guide the hybrid operation, and prevents the need for the ionizing radiation of fluoroscopy.     

Immunohistochemistry as a quick screening method for clinical detection of BRAF(V600E) mutation in melanoma patients

With the increased uses of targeted therapeutics, diagnostic detection of target mutations becomes essential for the effective clinical applications of targeted therapeutics. Currently, there are two types of methods detecting target mutations in clinics: one is based on DNA sequence and the other uses the newly developed mutation-specific antibodies recognizing mutated proteins. Each method has its own advantages and disadvantages. Here, we explored the sensitivity and specificity of a new commercially available BRAF(V600E) mutation-specific mouse monoclonal antibody. Using routine manual immunohistochemistry (IHC), we tested tumor tissues from 38 melanoma patients. For those melanoma tissues with abundant endogenous melanin, we pretreated the tumor tissues with 3 % hydrogen peroxide to remove melanin for reliable signal detection. We also performed DNA sequencing and ARMS-PCR analyses for these 38 tumor samples. Comparing to the results from DNA-based detection methods, the IHC method with this BRAF(V600E) mutation-specific antibody displayed 100 % sensitivity and 92.9 % specificity. Hence, this IHC detection is sensitive for clinic uses as a simple, fast, inexpensive, and reliable method to screen cancer patients for the BRAF(V600E) mutation and could be easily adapted for use in most hospital pathology laboratories.     

An efficient semi-synthesis of 1-hydroxyl oleanolic acid analogs

An efficient route for the semi-synthesis of either 1α- or 1β-OH epimers of 1-hydroxy-3-deoxyolean-12-en-28-oic acid (1), 6–8 steps from oleanolic acid is reported. The synthesis involves stereoselective formation of α,β-unsaturated epoxy ketone and subsequent Wharton reaction as key steps, offering a new access to the 1-O-substituted oleanolic acid-type pentacyclic triterpenoids.     

不同浓度布比卡因等比重椎管内麻醉应用于PPH术的临床观察

目的:观察不同浓度布比卡因等比重椎管内麻醉(腰麻)对吻合器痔状黏膜环切术(procedure for prolapse andhemorrhoids,PPH)的影响。方法:将90例ASA I～Ⅱ级择期行PPH术的男性混合痔患者随机分为布比卡因高、中、低浓度3组,每组30例,分别注入0.50%、0.25%和0.15%的布比卡因。观察3组麻醉前后10 min血压、心率、血氧饱和度的变化,感觉神经阻滞起效时间和阻滞最高平面,运动神经阻滞程度和阻滞持续时间,术中肛门松弛度和直肠牵拉反射情况。随访术后不良反应。结果:3组感觉神经阻滞起效时间、阻滞最高平面、直肠牵拉反射差异均无统计学意义(P>0.05),高浓度组运动神经阻滞程度、持续时间以及尿潴留发生率均高于另外2组(P<0.05和P<0.01);低浓度组术中肛门松弛度低于高浓度组(P<0.05)。结论:0.25%布比卡因等比重腰麻可满足PPH术的需求。     

外阴接触性皮炎的发生及正确评估

女性外阴瘙痒是皮肤科及妇产科门诊的常见症状,外阴接触性皮炎是最常引起外阴瘙痒症状的原因之一。文中就外阴皮肤对于接触性刺激及过敏的敏感性,刺激性接触性皮炎(irritant contact dermatitis,ICD)及过敏性接触性皮炎(allergic contact dermatitis,ACD)的发病情况,外阴药物接触性皮炎及如何正确认识和评估外阴接触性皮炎等方面进行综述。     

推拿对颈椎病患者外周血中TNF-α的影响

目的探讨推拿对颈椎病患者外周血中TNF-α的影响。方法将100例颈椎病患者随机分成推拿组40例、颈舒片组30例和塞来昔布组30例。推拿组采用推拿疗法,颈舒片组采用口服颈舒片,塞来昔布组采用口服塞来昔布。连续治疗14天为1疗程。观察TNF-α水平的变化及SF-MPQ评分变化。结果治疗前3组患者TNF-α水平比较,差异无显著性(P>0.05);治疗后3组TNF-α水平与治疗前比较均明显下降(P<0.05)。治疗后推拿组TNF-α水平与颈舒片组或塞来昔布组比较,差异有显著性(P<0.01)。结论推拿比口服颈舒片或塞来昔布更好地降低TNF-α的水平,对颈椎病患者疼痛有明显干预作用。     

Etanercept对糖尿病牙周炎大鼠糖尿病病情的影响

目的 观察TNF-α抑制剂Etanercept对实验性糖尿病牙周炎大鼠糖尿病病情的影响.方法 取12周龄wistar大鼠30只.腹腔内一次性注射STZ诱导糖尿病.结扎糖尿病大鼠牙颈部建立牙周炎模型.成模后的糖尿病牙周炎大鼠均分为对照组(给予安慰剂皮下注射)和实验组[给予Etanercept(25mg,2次/周)皮下注射].观察比较两组大鼠的牙周组织和血糖、糖化血清蛋白(GSP)的变化.结果 对照组大鼠可见深牙周袋,牙槽骨表面可见破骨细胞和骨吸收陷窝;实验组大鼠牙龈上皮固有层炎症浸润减轻,牙槽骨表面可见少量破骨细胞.对照组大鼠血糖和GSP值呈上升趋势,第8周血糖到达到(23.53±4.97)mmoI,L;GSP值达到(296.40±57.43)μ mol/L;实验组大鼠第2、4、8周时的血糖和GSP值均明显低于对照组(P<0.05),第8周达到最佳治疗效果,血糖值回落至(10.13±2.05)mmol/L,GSP值降为(143.89±24.86)μ mol/L.结论 Etanercept能明显抑制糖尿病牙周炎大鼠的糖尿病进程,尤以远期效果为好.