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Elisa Danese Marco Benati Fabian Sanchis-Gomar Cantor Tarperi Gian Luca Salvagno Elisa Paviati 《Scandinavian journal of clinical and laboratory investigation》2018,78(3):165-170
A specific subset of micro RNAs (miRs), including miR-133 and miR-206, is specifically expressed in muscle tissue, so that they are currently defined as muscular miRs (myomiRs). To further elucidate the role of myomiRs in muscle biology, we measured miR-133a and miR-206 in plasma of 28 middle-age recreational athletes. The study population consisted of 28 middle aged, recreation athletes (11 women and 17 men; mean age, 46?years) who completed a 21.1?km, half-marathon. The plasma concentration of miR-133a and miR-206, the serum concentration of creatine kinase (CK) and high-sensitivity (HS) cardiac troponin T (cTnT), as well as capillary lactate, were measured before and immediately after the run. The median serum concentration of total CK (257 versus 175?U/L; p?.001), cTnT (17.8 versus 5.6?ng/L; p?.001), and the plasma values of both miR-133a (4.22 versus 0.64?×?10?4; p?.001) and miR-206 (1.36 versus 0.63?×?10?4; p?=?.001) were considerably increased immediately after the half-marathon run. In multivariate analysis only post-exercise capillary lactate was found to be independently associated with running time. A significant and independent correlation was observed between plasma variations of the two miRs, but not with other physiological or laboratory parameters. The results of this study suggest that the biological significance of miR-133a and 206 variation after middle-distance running parallels but not overlaps the release of biomarkers of nonspecific tissue damage. Enhanced plasma values of these myomiRs may hence reflect a physiological response to high-intensity and/or prolonged exercise rather than tissue injury. 相似文献
74.
F.?BüngerEmail author D.?Feierabend P.?Storch R.?Kalff R.?Reichart 《Schmerz (Berlin, Germany)》2018,32(2):121-127
Background
Subcutaneous peripheral nerve field stimulation (sPNFS) is an established procedure for the treatment of chronic localized neuropathic pain of peripheral origin. The treatment of nummular headache primarily focuses on conservative methods with limited prospects of success. The role of sPNFS in the treatment of nummular headache has not been investigated as yet.Question
Is the sPNFS an option in the management of nummular headache?Materials and methods
In addition to a summary of established methods in the treatment of nummular headache, sPNFS as a possible form of therapy is discussed.Results
A positive effect of sPNFS in terms of the treatment of nummular headache is shown.Discussion
sPNFS stimulates free subcutaneous nerves and transmits a pleasant form of paraesthesia in the area of pain. If regular conservative therapy has already been exhausted, then sPNFS might be an effective new option in the treatment of nummular headache. sPNFS is a minimally invasive and low-risk procedure. However, the high treatment cost and restrictions regarding fitness to undergo MRI are points of criticism. Further studies are needed to define its potential and role in the treatment of nummular headache.75.
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Anna Lam MD Thomas Küffer MSc Lukas Hunziker MD Nikolas Nozica MD Babken Asatryan MD PhD Florian Franzeck MD Antonio Madaffari MD Andreas Haeberlin MD PhD Aline Mühl MSc Helge Servatius MD Jens Seiler MD Fabian Noti MD Samuel H. Baldinger MD Hildegard Tanner MD Stephan Windecker MD Tobias Reichlin MD Laurent Roten MD 《Journal of cardiovascular electrophysiology》2021,32(6):1610-1619
78.
The antiretroviral activity of APOBEC3 is inhibited by the foamy virus accessory Bet protein 总被引:13,自引:0,他引:13
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79.
The endothelium plays a pivotal role in the theological regulation of blood flow by the secretion of vasoactive factors.
The interaction between shear forces and the endothelium is determined by the mechanical properties of the endothelial cell
layer which are associated with intercellular junctions. Cell-cell contacts could therefore modulate the secretion of vasocative
factors in response to theological stimuli. We investigated the relationship between intercellular junctions and the secretion of the vasoconstrictor peptide endothelin and the coagulation co-factor von Willebrand factor (vWF). Human
umbilical vein endothelial cells (HUVECs) were used as in vitro endothelial model system. Intercellular junctions were reversibly
disrupted by calcium chelation or hypertonic stress; alternatively, the formation of intercellular junctions was inhibited
by culturing the cells in suspension or by plating them in the presence of an inhibitory anti-VE-cadherin antibody. The opening
of intercellular junctions was verified by assessing transmonolayer electrical resistance (TMR) and immunofluorescence morphology.
The concentration of endothelin and vWF was measured in the cell culture supernatants using specific ELISAs. The secretion
of endothelin was inhibited by EGTA (5 mM) and stimulated by incubation with tumor necrosis factor α (TNFα, 40 ng/ml). Treatment
with hypertonic medium (glycerol, 1200 mosmol/l) for 10 minutes opened intercellular junctions and markedly reduced the secretion
of endothelin. HUVECs in suspension culture did not secrete endothelin and failed to respond to TNFα, but readily resumed
these functions upon forming a new monolayer on plastic. The reconstitution of intercellular junctions after suspension culture
could be inhibited using a specific anti-VE-cadherin antibody. This antibody, but not a non-specific anti-humanIgG antibody
reduced endothelin secretion. The secretion of von Willebrand Factor was less dependent on intercellular junctions. The opening
of intercellular junctions did not induce cell death, since the cells continued to exclude trypan blue. The results of this
study suggest a novel and potentially pathophysiologically/clinically relevant correlation between intercellular junctions
and the secretion of endothelin in endothelial cells.
Received: 17 December 1999, Returned for revision: 20 January 2000, Revision received: 14 February 2000, Accepted: 2 March
2000 相似文献
80.
Activation of c-K-ras mutations in human gastrointestinal tumors 总被引:3,自引:0,他引:3
Arber N Shapira I Ratan J Stern B Hibshoosh H Moshkowitz M Gammon M Fabian I Halpern Z 《Gastroenterology》2000,118(6):1045-1050
BACKGROUND & AIMS: Ras genes are the most frequently detected oncogenes in human malignancies. Data regarding the frequency of c-K-ras mutations in esophageal, gastric, and small bowel tumors are limited and controversial. METHODS: DNA was extracted from 262 formalin-fixed, paraffin-embedded sections of gastrointestinal samples and tumors, including Barrett's esophagus, esophageal squamous cell carcinomas and adenocarcinomas, and small and large bowel adenomas and adenocarcinomas. The presence of c-K-ras codon 12 mutations was determined using a nonradioactive polymerase chain reaction-based restriction fragment length polymorphism assay. RESULTS: c-K-ras mutations were detected in 1 of 39 (2%) patients with Barrett's esophagus, 1 of 21 (5%) adenocarcinomas, 0 of 27 squamous cell carcinomas of the esophagus, and 1 of 32 (3%) gastric adenocarcinomas. It was also present in 8 of 20 (40%) and 10 of 28 (36%) small bowel adenomas and adenocarcinomas, respectively. Similar numbers were observed in 10 of 25 (40%) large bowel adenomas and 11 of 30 adenocarcinomas (37%). Mutations were not associated with age, gender, histology, grade, stage, location, or mortality. CONCLUSIONS: The frequency of codon 12 c-K-ras mutations in small and large bowel tumors is approximately 10-fold higher than that of tumors in the upper gastrointestinal tract. 相似文献