Comparing the efficacy of different intra-articular injections for knee osteoarthritis: A network analysis

Background:The findings on the effectiveness of platelet-rich plasma, ozone, and hyaluronic acid in the treatment of osteoarthritis of the knee are controversial, and the existing original studies and meta-analyses are mostly comparisons of a single joint cavity injection method, lacking direct and indirect comparisons of different drugs in the joint cavity. The lack of direct and indirect comparisons of different drugs in the joint cavity makes it difficult to have a clearer and more comprehensive understanding of joint cavity injection methods. In this study, the efficacy of platelet-rich, ozone, sodium hyaluronate, and combined knee cavity injections were compared directly or indirectly using a reticulated meta-analysis in this field, and the efficacy of treatment measures was ranked to provide more comprehensive and reliable evidence-based clinical evidence for the selection of knee cavity injections in osteoarthritis of the knee.Objective:To compare the effects of platelet-rich plasma, ozone, and sodium glassate injection interventions on the efficacy of osteoarthritis of the knee through reticulated Meta-analysis, and to comprehensively compare the clinical effectiveness of platelet-rich plasma, ozone, and sodium glassate injection joint cavity injection for the treatment of osteoarthritis of the knee.Methods:The PubMed, CBM, CNKI, VIP, and Wan-Fang databases were searched for information on the effectiveness of platelet-rich plasma, ozone, and sodium vitrate injection for the comparative treatment of osteoarthritis of the knee, with a search time frame of each database from the date of creation to July 20, 2021. Two investigators independently screened the literature, extracted data according to inclusion and exclusion criteria, and evaluated the quality of the literature in parallel. Statistical analysis was performed using Stata 16.0 software to compare the differences in the efficacy of each treatment measure using the ratio and 95% confidence interval as effect indicators and to rank the efficacy.Results:Thirty-three RCTs with 7003 patients with osteoarthritis of the knee were included, involving 5 therapeutic measures. Meta-analysis showed that the efficacy of platelet-rich plasma injection was superior to both ozone and hyaluronic acid therapies. Hyaluronic acid+ozone and platelet-rich plasma+hyaluronic acid were both superior to ozone and hyaluronic acid monotherapy. The differences in efficacy between hyaluronic acid and ozone compared with platelet-rich plasma were statistically significant, and the differences in efficacy between the 2 combination therapies (platelet-rich plasma+hyaluronic acid, hyaluronic acid+ozone) and the 3 monotherapies (platelet-rich plasma, ozone, hyaluronic acid) were statistically significant. Platelet-rich plasma+hyaluronic acid, hyaluronic acid+ozone compared with 3 monotherapies (platelet-rich plasma, ozone, hyaluronic acid) were statistically significant, except for the difference in efficacy with platelet-rich plasma, which was not statistically significant, indicating that this platelet-rich plasma+hyaluronic acid and Hyaluronic acid+ozone combination therapy was superior to monotherapy. Also, the efficacy of platelet-rich plasma was better than hyaluronic acid and ozone and the difference was statistically significant, indicating that platelet-rich plasma was more effective than ozone and sodium glass in the treatment of osteoarthritis of the knee in monotherapy.Conclusion:It is believed that in the course of clinical practice, hyaluronic acid+ozone or platelet-rich plasma+hyaluronic acid combination therapy or platelet-rich plasma therapy can be preferred for patients with osteoarthritis of the knee.     

不同出生体重婴儿0～6月体重生长的前瞻性研究

目的：比较0～6月龄不同出生体重（Birth weight,BW）婴儿体重的生长情况,为不同BW婴儿早期的临床保健指导提供参考依据。方法：2010年5月至2011年9月,在重庆医科大学附属儿童医院儿保科建立前瞻性队列,分为巨大儿组、正常BW儿（Normal birth weight,NBW）组和早产低BW儿（Preterm low birth weight,PLBW）组。比较不同BW婴儿6月龄时年龄的体重Z值（Weight for age Z-score,WAZ）、超重发生率及0～6月龄体重的生长速度（ΔWAZ）。结果：（1）PLBW组86例,NBW组154例,巨大儿组107例。（2）6月龄时PLBW组、NBW组和巨大儿组WAZ均值分别是-0.60±1.12、0.46±0.83 和1.17±0.92,P＜0.000 1。（3）0～6月龄PLBW组、NBW组和巨大儿组ΔWAZ均值分别是2.41±1.01、0.48±0.83和-0.56±0.95,P＜0.000 1。（4）PLBW组、NBW组及巨大儿组3组超重的发生率分别是4.65%、6.49%和19.63%。（5）早产儿配方奶喂养者和足月儿配方奶喂养者ΔWAZ的均值明显大于母乳喂养者,P=0.008 5;NBW组和巨大儿组中,足月儿配方奶喂养ΔWAZ的均值高于母乳喂养者,但无明显统计学差异。结论：（1）生后6个月巨大儿体重持续高水平生长,超重发生率亦高,而生长速度较NBW及PLBW慢;（2）母乳喂养的巨大儿、NBW和PLBW生后6月内体重的生长速度较配方奶喂养者慢,超重的发生率较配方奶喂养者低。     

颧骨缝牵张成骨过程中组织超微结构变化的研究

目的:观察天然骨缝牵张成骨过程中组织和细胞超微结构的变化。方法:对牵张成骨的缝区组织经过系列处理,进行超薄切片、透射电镜观察。结果:间充质细胞在牵引早期大量增生,并不断分化为成纤维细胞和成骨细胞,后2种细胞的超微结构显示出具有活跃的合成和分泌功能。1周标本成纤维细胞沿牵引力的长轴方向排列,胞核增大,在其周围包绕着发达并扩张的内质网系统;3周标本大量增生活跃的成骨细胞和成纤维细胞,成骨细胞核仁增大、粗面内质网扩张、核糖体丰富、线粒体增多、富含紧密排列的嵴;5、8周标本中骨细胞形成并发育成熟,骨基质逐渐矿化,清晰可见新形成的胶原纤维、哈氏管,以及骨基质矿化的过程。结论:三维牵张过程中,成纤维细胞、成骨细胞活跃,缝区形成新骨。     

海洛因依赖者记忆力与氧化应激的关系

目的：探讨男性海洛因依赖者（MPHD）记忆力改变及其与氧化应激的关系。方法：对140例MPHD及75名健康者对照进行韦氏记忆量表（WMS）评估和氧化应激指标血清一氧化氮（NO）、丙二醛（MDA）、超氧化物歧化酶（SOD）、维生素C（VC）及总抗氧化能力（T-AOC）的检测。结果：MPHD组WMS的测验成绩显著差于对照组（P〈0．05）;MPHD组血清MDA水平高于对照组（P〈0．05）,T-AOC、VC、SOD水平低于对照组（P〈0．05或P〈0．01）;随戒断时间延长,MPHD组的记忆商数（MQ）有升高的趋势（P〉0．05）,血清MDA含量有下降的趋势（P〈0．01）,T-AOC、SOD含量有升高的趋势（P〈0．05）。MPHD组血清NO及MDA水平与WMS多个因子呈负相关（P〈0．05或P〈0．01）,T-AOC、VC及SOD水平与WMS多个因子呈正相关（P〈0．05或P〈0．01）。结论：海洛因依赖者存在明显的记忆力损害与氧化抗氧化反应失衡;其记忆力损害可能与氧化应激有关,血清NO、MDA、SOD、VC、T-AOC可能是与记忆力密切相关的氧化应激指标。     

奎硫平治疗精神分裂症患者的疗效及生活质量影响

目的:探讨奎硫平与氯丙嗪对精神分裂症患者的疗效及生活质量的影响。方法:对62例精神分裂症患者,随机使用奎硫平和氯丙嗪治疗,疗程3个月。采用阳性与阴性症状量表(PANSS)评定疗效,采用治疗中出现的症状量表(TESS)评定不良反应,以生活质量综合评定问卷(GQOLI-74)评定生活质量。结果:两组均有显著疗效,奎硫平可显著提高生活质量。两组间无显著差异(P>0.05)。氯丙嗪组不良反应比奎硫平组多。结论:奎硫平和氯丙嗪对精神分裂症疗效相当,奎硫平不良反应较少,对提高患者生活质量的效果明显优于氯丙嗪。     

亚甲基四氢叶酸还原酶C677T基因多态性与2型糖尿病合并冠心病的关系

目的 研究同型半胱氨酸代谢关键酶亚甲基四氢叶酸还原酶（methylenetetrahydrofolate reductase,MTHFR）基因C677T多态性与糖尿病合并冠心病发病的关系,探讨MTHFR是否为糖尿病合并冠心病的易感基因。方法 研究对象包括105名糖尿病合并冠心病的患者（合并组）、88名单纯糖尿病患者（糖尿病组）和91名健康人。应用聚合酶链反应-限制性内切酶长度多态性方法（PCR—RFLP）检测MTHFR C677T基因多态性,同时检测血浆同型半胱氨酸（homocysteine,Hcy）、叶酸、维生素B12、各种血脂。结果 合并组与糖尿病组比,等位基因频率差异有统计学意义（Х^2=6．8,P〈0．05）,合并组T等位基因的OR值为1．638（95％ CI,1．082～2．479）,基因型频率差异亦有统计学意义（Х^2=5．481,P〈0．05）。Logistic回归分析显示MTHFR 677携带T基因（CT＋TT）的OR值为2．68（95％ CI,1．233—5．824）。结论 MTHFR 677携带T基因与2型糖尿病合并冠心病发生独立相关。检测MTHFR 677位点基因特点可能为糖尿病合并冠心病的预防以及个体化治疗提供新思路、新方法。     

项目管理在提高门诊肠镜患者肠道准备合格率中的应用

目的 探讨项目管理在提高门诊肠镜患者肠道准备合格率中的应用效果.方法 成立项目管理团队,对门诊肠镜患者肠道准备合格率进行调查,找出影响合格率亟待改进的问题,实施项目管理,项目管理运用后再次进行调查,将调查结果与实施前进行比较.结果 项目管理实施后门诊肠镜患者肠道准备合格率显著高于实施前(P<0.01).结论 实施项目管...     

保湿眼膜对眼周皮肤滋润作用的评价

目的：评价保湿眼膜产品对眼周皮肤的滋润护理作用。方法：采用皮肤水分仪Corneometer检测保湿眼膜产品对眼周皮肤水分含量的影响。结果：双眼外眦外侧使用含保湿眼膜的组合产品2．4、8h后皮肤水分含量比空白对照区升高．差异有统计学意义（P〈0．05）;使用1、2、3周后的皮肤水分含量仍比使用前高,差异有统计学意义（P〈0．05）;而未使用保湿眼膜的对照产品在3周内对皮肤水分含量的作用不明显（P〉0．05）。结论：眼周皮肤的护理不仅需要使用普通的护肤产品,更需要使用针对眼周皮肤生理特性的保湿眼膜产品,才能有效地滋润眼周皮肤,起到保养护理的效果。     

成人皮肌炎伴发恶性肿瘤的相关因素分析

目的:探讨成人皮肌炎(DM)患者伴发肿瘤的相关因素。方法:回顾性分析2016年1月至2018年12月在我院皮肤科确诊为成人DM的患者,对其临床特征、实验室检查共16项指标进行统计分析。结果:共分析114例成人DM患者,其中伴发肿瘤21例(18.4%),以肺癌(6例,28.57%)最常见。单因素分析结果显示,单纯DM组和DM伴发肿瘤组年龄、性别、吞咽困难、白蛋白水平和红细胞沉降率(ESR)比较差异有统计学意义(P<0.05)。将上述单因素分析差异有统计学意义的指标纳入多因素Logistic分析结果显示,性别和ESR是影响成人DM伴发肿瘤的危险因素。结论:应考虑将男性DM患者,或者ESR≥35 mm/h纳入成人DM伴发恶性肿瘤的危险因素,加强DM早期的肿瘤筛查工作。     

Vitamin D Enhances Neutrophil Generation and Function in Zebrafish (Danio rerio)

Vitamin D (VD) is a major regulator of calcium metabolism in many living organisms. In addition, VD plays a key role in regulating innate and adaptive immunity in vertebrates. Neutrophils constitute an important part of the first line of defense against invading microbes; however, the potential effect of VD on neutrophils remains elusive. Thus, in this study zebrafish in different developmental stages were utilized to identify the potential role of VD in the basal homeostasis and functions of neutrophils. Our results showed that addition of exogenous VD₃ promoted granulopoiesis in zebrafish larvae. Reciprocally, neutrophil abundance in the intestine of adult zebrafish with a cyp2r1 mutant, lacking the capacity to 25-hydroxylate VD, was reduced. Moreover, VD-mediated granulopoiesis was still observed in gnotobiotic zebrafish larvae, indicating that VD regulates neutrophil generation independent of the microbiota during early development. In contrast, VD was incapable to influence granulopoiesis in adult zebrafish when the commensal bacteria were depleted by antibiotic treatment, suggesting that VD might modulate neutrophil activity via different mechanisms depending on the developmental stage. In addition, we found that VD₃ augmented the expression of il-8 and neutrophil recruitment to the site of caudal fin amputation. Finally, VD₃ treatment significantly decreased bacterial counts and mortality in zebrafish infected with Edwardsiella tarda (E. tarda) in a neutrophil-dependent manner. Combined, these findings demonstrate that VD regulates granulopoiesis and neutrophil function in zebrafish immunity.