全文获取类型
收费全文 | 415篇 |
免费 | 31篇 |
国内免费 | 105篇 |
专业分类
儿科学 | 27篇 |
妇产科学 | 5篇 |
基础医学 | 43篇 |
口腔科学 | 34篇 |
临床医学 | 102篇 |
内科学 | 99篇 |
皮肤病学 | 6篇 |
神经病学 | 8篇 |
特种医学 | 69篇 |
外科学 | 23篇 |
综合类 | 37篇 |
预防医学 | 10篇 |
眼科学 | 5篇 |
药学 | 51篇 |
中国医学 | 1篇 |
肿瘤学 | 31篇 |
出版年
2021年 | 4篇 |
2020年 | 2篇 |
2019年 | 3篇 |
2018年 | 5篇 |
2017年 | 6篇 |
2016年 | 7篇 |
2015年 | 11篇 |
2014年 | 6篇 |
2013年 | 16篇 |
2012年 | 6篇 |
2011年 | 12篇 |
2010年 | 17篇 |
2009年 | 17篇 |
2008年 | 4篇 |
2007年 | 58篇 |
2006年 | 11篇 |
2005年 | 24篇 |
2004年 | 12篇 |
2003年 | 9篇 |
2002年 | 13篇 |
2001年 | 5篇 |
2000年 | 11篇 |
1999年 | 6篇 |
1998年 | 45篇 |
1997年 | 35篇 |
1996年 | 38篇 |
1995年 | 17篇 |
1994年 | 22篇 |
1993年 | 14篇 |
1992年 | 10篇 |
1991年 | 3篇 |
1990年 | 11篇 |
1989年 | 13篇 |
1988年 | 14篇 |
1987年 | 12篇 |
1986年 | 12篇 |
1985年 | 5篇 |
1984年 | 3篇 |
1982年 | 3篇 |
1981年 | 4篇 |
1980年 | 3篇 |
1977年 | 4篇 |
1976年 | 6篇 |
1975年 | 3篇 |
1974年 | 1篇 |
1973年 | 1篇 |
1972年 | 1篇 |
1971年 | 1篇 |
1965年 | 1篇 |
1960年 | 1篇 |
排序方式: 共有551条查询结果,搜索用时 15 毫秒
131.
目的:提出了一种可用于CT仿真结肠镜和其他空腔性结构可视化的薄壁快速体绘制方法。
方法:基于图像分割和三维形态学运算,能够去除空腔中积水的影响,并加快体绘制的速度。利用VGLR体绘制工具包,实现高效的内窥体绘制计算。
结果:在一组真实的CT结肠数据中,实现了图像分割、形态学运算及快速实时体绘制,体绘制速度达到原来的3倍。
结论:这一技术能加快空腔结构内窥体绘制的速度,去除肠道内积水的影响,提高可视化效果。 相似文献
132.
Murine JAK3 is preferentially expressed in hematopoietic tissues and lymphocyte precursor cells 总被引:2,自引:2,他引:2
To elucidate the role of cytokine receptor signal transduction in T- cell development, we have investigated the expression pattern and biochemical characteristics of the murine Janus family tyrosine kinase, JAK3. Previous studies have shown that JAK3 is expressed in lymphoid and myeloid tumor cell lines and in a small number of lymphoid tissues. To further characterize JAK3 expression, we used a quantitative polymerase chain reaction approach to compare JAK3 mRNA levels at multiple stages of T-cell differentiation and in a broad range of mouse tissues. These studies, in conjunction with analyses of JAK3 protein expression, show that the highest levels of JAK3 are in adult, 2-week- old, and fetal thymus, followed by somewhat lower levels in bone marrow, spleen, fetal liver, and adult CD4-CD8- thymocytes. We also show that different forms of JAK3 mRNA arise by alternative splicing. Finally, our biochemical studies show that the JAK3 kinase domain, but not the pseudo-kinase domain, has tyrosine kinase activity and, furthermore, that JAK3 kinase activity is abolished by an amino acid substitution of the conserved lysine in the kinase domain (K851R). These studies show that JAK3 expression is profoundly skewed to hematopoietic and lymphoid precursor cells, strongly suggesting a role for JAK3 in hematopoiesis and T- and B-cell development. 相似文献
133.
Human lymphotoxin has at least equal antitumor activity in comparison to human tumor necrosis factor but is less toxic in mice 总被引:5,自引:0,他引:5
Because of the severe toxicity of systemically applied tumor necrosis factor (TNF) in cancer patients, considerable efforts have been made to construct mutant TNF molecules, which retain antitumor activity, but display less toxicity. We compared tumor suppression in relation to the toxic effects of human TNF and human lymphotoxin (LT) in mice. The genes for these two cytokines were expressed in Chinese hamster ovary (CHO) cells. Intraperitoneal injection of parental and gene modified CHO cell lines producing similar amounts of biologically active TNF or LT, respectively, into nude mice showed that CHO-TNF cells killed the mice more rapidly than parental cells, but that CHO-LT tumor bearing mice lived significantly longer than mice injected with parental cells. Injection of the cells subcutaneously into severe combined immunodeficiency (SCID) mice allowed direct comparison of tumor suppression and toxic effects of the two cytokines. Both TNF and LT produced by the tumor effectively suppressed tumor growth by an indirect mechanism, LT being at least as effective as TNF. However, mice bearing CHO-TNF cells either died rapidly or developed cachexia, as shown by weight loss. In contrast, mice injected with CHO-LT cells never rapidly died and became cachectic much later than CHO-TNF cell injected animals, though serum levels of LT were higher than those of TNF. Analysis of soluble forms of TNF receptors (TNF-R1 and TNF-R2) in sera of tumor bearing mice showed that soluble TNF-R1 was downregulated in both CHO-TNF and CHO-LT, in comparison with CHO-neo cell injected mice and to normal SCID mice. The soluble form of TNF-R2 was induced by CHO cell lines. In CHO-TNF cell injected SCID mice, serum levels were significantly increased, whereas in mice injected with CHO-LT cells, serum levels of soluble TNF-R2 were decreased. Together, our results show a higher therapeutic index of LT compared with TNF. 相似文献
134.
Association between gelatinase release and increased plasma membrane expression of the Mo1 glycoprotein 总被引:7,自引:0,他引:7
Mo1, a glycoprotein heterodimer (gp 155,95) that functions as an adhesion promoting molecule and as the C3bi receptor of human myeloid cells, is expressed in increased amounts in the plasma membrane after exposure of polymorphonuclear leukocytes (PMNs) to various stimuli. Previous studies have suggested that secondary granules represent an intracellular pool of Mo1 that, upon degranulation, fuse with the plasma membrane resulting in a tenfold increase in surface expression of Mo1. To determine the intracellular location of Mo1, we monitored Mo1 expression by immunofluorescence and compared it to the release of myeloperoxidase (MPO, a marker for the primary granules), vitamin B12 binding protein (B12BP, secondary granules), and gelatinase (gelatinase- containing organelles) following exposure to various stimuli. Human neutrophils stimulated with 20 mmol/L fluoride for 16 minutes exhibited a twofold increase in Mo1 expression and gelatinase release but no enhanced release of primary or secondary granular contents. In a similar fashion, incubation of cells at 37 degrees C for five minutes with 7.5 X 10(-9) to 10(-6) mol/L N-formyl-methionyl-leucyl- phenylalanine (FMLP) resulted in significant increases in both surface Mo1 expression (three- to fivefold) and gelatinase release (five- to eightfold) without significant release of either MPO or B12BP. In addition, both the fluoride and FMLP experiments demonstrated that Mo1 up-modulation alone is not sufficient to activate superoxide (O2-) production. These data indicate that at least one intracellular storage pool of Mo1 is the gelatinase-containing organelles and that their fusion with the plasma membrane results in increased expression of Mo1 on the cell surface. 相似文献
135.
Carbonyl iron therapy for iron deficiency anemia 总被引:2,自引:0,他引:2
To determine if elemental carbonyl iron powder is safe and effective therapy for iron deficiency anemia, 20 nonanemic and 32 anemic volunteers were studied. Single doses of 1,000 to 10,000 mg of carbonyl iron (15 to 150 times the 65 mg of iron in the usual dose of ferrous sulfate) were tolerated by nonanemic volunteers with no evidence of toxicity and only minor gastrointestinal side effects. Anemic volunteers (menstruating women who had previously donated blood) were treated with several regimens providing 1,000 to 3,000 mg of carbonyl iron daily in one to three doses for 8 to 28 days. After 12 weeks anemia was corrected in 29 of 32 patients, and serum ferritin was greater than 12 micrograms/L in 14. Hemoglobin regeneration proceeded at a rate similar to that described for therapy with oral iron salts and parenteral iron dextran. There was no evidence of hematologic, hepatic, or renal toxicity, but mild gastrointestinal side effects occurred in a majority of anemic volunteers. Carbonyl iron is an effective, inexpensive treatment for iron deficiency anemia, is accompanied by tolerable side effects and may have an advantage over therapy with iron salts by substantially reducing or eliminating the risk of iron poisoning in children. 相似文献
136.
137.
138.
139.
140.